꼬리줄나비고기, Chaetodon wiebeli의 초기발생

이치훈,김병호,노섬,이영돈 제주대학교 해양과환경연구소 2004 해양과환경연구소 연구논문집 Vol.28 No.2

The development of eggs and larvae of the butterflyfish. Chaetodon wiebili were observed. Fertilized eggs were spherical. colorless. and driven into gelatinous masses. They measured 0.75±0.18 in diameter and not contain an oil globule. Hatching taken place 61.0 hours after two celled stage at water temperature 25.6-27.7℃. Newly hatched larvae measured 0.91 nm in total length and had an ovoid yolk of 0.65 nm in longest diameter. In 72 hours after hatching. the larvae grew 1.72 nm in total length. The mouth and anus were open and the melanophore pigments appeared above the eyes. The pectoral fins are formed and the larvae began to swim actively.

SGML을 이용한 제안서, 보고서 관리시스템 기술 개발

전병현,한치근,이상훈 경희대학교 산학협력기술연구원 1997 산학협력기술연구논문집 Vol.3 No.-

We designed an SGML-Based Proposal and Report Management System in this paper. In this system, SGML files are stored in a database and can be searched using keywords. Hence, the documents can be handled efficiently. The relational database structure is recommended for the system and also a hybrid approach is discussed for multimedia information in the text. Functions for each module of the system are defined and conceptual and practical design issues are provided.

주요 우울장애에서 Cytotoxic T Lynphocyte Antigen(CTLA-4) 유전자의 다형성

전태연,배치운,김병균,채정호,박원명,김광수,유태열,한훈 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 세포의 면역 기능 조절에 관여하는 CTLA-4의 유전자 다형성을 분석하여 주요 우울장애와의 면역 유전학적 관련성을 알아보고자 하였다. 방 법 : DSM-Ⅳ진단 기준에 따라 주요 우울장애로 진단된 환자 77명을 선정하였으며 가톨릭대학교 의과대학 조혈모세포은행에서 보유하고 있는 149명의 정상 한국인 자료를 대조군으로 사용하였다. 전혈에서 DNA를 추출하였으며 중합효소반응으로 CTLA-4유전자 exon 1 부위를 증폭한 후 SSCP방법으로 유전?? 형별 분석하였다. 결 과 : 대조군과 주요 우울장애 환자군 사이의 CTLA-4 유전자형과 대립유전자의 발현 빈도를 비교시 유의한 차이가 없었다. 결 론 : 본 연구 결과 exon 1 CTLA-4 유전자형 및 대립유전자의 발현빈도는 주요 우울장애 환자군과 정상 대조군 사이에 차이가 없어, exon 1 영역은 후보 유전자로서의 가능성이 없었다. 향후 연구에서는 다양한 변인들에 따라 대상군을 임상적으로 동질성을 지닌 집단으로 세분하여 조사대상 유전자와의 관련성에 대한 연구가 이루어져야 하겠다. Objectives : This study was carried out to explore the relationship between major depressive disorder and CTLA-4 which is related to the immunologic function such as T cell regulation. Methods : Among the korean patients diagnosed as major depressive disorder according to DSM-Ⅳ, 77 patients without neurological illness, hormonal disorder, or comorbid mental illness were selected. The stored data of 149 normal Koreans from the Catholic Hemopoietic Stem Cell Bank of Korea, were used as a normal control group. The data of Korean control group were compared with those of the studies of different ethnics. DNA was extracted from whole blood and the exon I region of CTLA-4 gene was amplified by polymerase chain reaction. Gene typing was performed by using SSCP and then, the results were assessed. Results : There were no significant differences in genotype frquencies of CTLA-4*G/G, CTLA-4*G/A, and CTLA-4*A/A between the patients with major depressive disorder and the control group in Korean population(48.1% vs 46.3%, 41.6% vs 39.6%, 10.3% vs 14.1%, respectively).There were no significant differences in allelic frequencies of CTLA-4*G and CTLA-4*A between the patients with major depressive disorder and the control group in Korean population(68.8% vs 66.1%, 31.2% vs 33.9%, respectively). Conclusion : Considering negative result for the association of the exon I polymorphism of CTLA-4 gene with major depressive disorder in this study, the exon I polymorphism does not appear to be possible candidate gene for major depressive disorder. Moreover, further systematic researches including diverse clinical variables would required.

씀바퀴 성분 중 Cynaroside의 약동태학적 연구

신영희,민병훈,이치호 慶星大學校 1998 論文集 Vol.19 No.1

We isolated cynaroside(luteolin-7-O-glucoside) from the whole plant of Ixeris dentata, and characterized pharmacokinetic properties of cynaroside. It was administered by intravenous injection, intraperitoneal injection and oral routes to male rats, and plasma concentrations of cynaroside were analysed by HPLC method. After iv injection of cynaroside with 5mg, 10mg, and 20 mg per kg of body weight, the area under concentration curve and doses showed a good correlationships. On the other hand, cynaroside hardly absorbed by oral administration route.

Modified Free Wrap-around flap을 이용한 수무지 재건술

최영화,황치원,정병훈,한명호 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

The thumb is the most important part in the hand and its loss results in severely diminished function of the hand for pinch and grasp. There are several microsurgical and other methods to reconstruct and amputated or lost thumb such as toe-to-thumb transfer1)2)9) as well as several varieties of flaps. Among them, a free wrap around flap taken from the big toe, including the nail and an iliac bone graft as the cone of the reconstruction was a quite satisfactory procedure, but there were some problems. We performed a free wrap around flap on traumatically missing thumb with modification to include all part of the distal phalanx without iliac bone graft. The resorption and atrophy of a grafted iliac bone, a constant problem with Morrison's original procedure8), could be prevented by the modified method. The donor site defects were covered by remained medial flap for weight bearing area first, and full thickness skin graft for remained defected area. The results were good cosmetically and functionally without significant problems.

서해산 돌가자미 Kareius bicoloratus의 초기발생시 수온과 염분의 영향

전제천,김치홍,정의영,이창훈,김병균 한국어류학회 2002 韓國魚類學會誌 Vol.14 No.3

본 연구는 돌 가자미(Kareius bicoloratus)의 난발생에 미치는 수온과 염분의 영향을 알아보지 위하여 수행되었다. 5단계의 수온 조건 (7, 10, 13, 16 및 19℃) 및 5단계의 염분 조건(25, 30, 35, 40psu 및 현장해수 염분 : 33.6psu)에서 수정란을 발생시켜 발생 속도와 부화율 측정하였다. 수정란의 발생속도는 수온이 높을수록 빨랐으며, 수온(T: ℃)과 각 발생 단계별 소요시간(h:hour) 사이의 관계를 직선 함수에 회귀시켰을 때 각 발생단계별 관계식은 다음과 같다. 8세포기 : 1/h = 0.01441T - 0.02728(r^(2) = 0.998) 낭배기 : 1/h = 0.00269T - 0.00319(r^(2) = 0.997) 안포형성기 : 1/h = 0.00171T - 0.00053(r^(2) = 0.998) 심장형성기 : 1/h = 0.00121T - 0.00018(r^(2) = 0.995) 부화기 : 1/h = 0.00101T - 0.00266)(r^(2) = 0.998) 이상의 관계식으로부터 추정된 돌 가자미의 난 발생이 개시되는 생물학적 영도는 1.3℃이었다. 8세포기, 낭배기, 안포형성기. 심장형성기 및 부화기까지의 적산수온은 각각 72.9, 356.7, 525.8, 735.2 및 1156.7℃로 계산되었다. 수온은 부화율에 큰 영향을 미치는 것으로 나타났다(p<0.001). 부화율이 수온이 증가할수록 감소하였으며, 7℃일 때 부화율이 62.0%로 가장 높았다. 수온이 16℃ 이상일 때 기형어의 출현율이 증가하였다(p<0.05). 염분 또는 부화율에 큰 영향을 미치고 있었다(p<0.001). 염분에 따른 부화율은 자연해수의 염분인 33.6psu에서 54.1%로 가장 높았고, 이보다 낮거나 높은 경우에는 부화율이 감소하였다. 특히, 염분이 30psu 미만으로 감소할 경우 기형어의 출현율 증가하였다(p<0.04). 돌 가자미의 난 발생 및 정상적인 부화를 위한 조건은 산란시기의 수온과 염분에 가까울수로고 좋은 것으로 판단된다. This study was conducted to determine the influences of water temperature and salinity on the embryonic development of the stone flounder, Kareius bicoloratus as a part of research on the artificial seedling production of the fish. Embryos were incubated under five different temperatures (7, 10, 13, 16 and 19℃) and five different salinities (25, 30, 35, 40 psu and the ambient salinity: 33.6 psu) until they hatched. The required times for embryonic development decreased with increase of water temperature. The relationships between the water temperature (T: ℃) and required times (h: hour) for the embryo to attain each develop-mental stage were obtained by linear regressions as follows; 8-cell stage : l/h = 0.01441T-0.02728 (r² = 0.998) 50%-epiboly stage : Uh = 0.00269T-0.00319 (r² = 0.997) Optic vesicle formation stage : l/h = 0.00171T-0.00053 (r² = 0.998) Heart formation stage : l/h = 0.00121T-0.00018 (r² = 0.995) Hatching stage : l/h = 0.00101T-0.00266 (r²= 0.998) From these equations, the biological minimum temperature for embryonic development of K. bicoloratus was estimated to be 1.3℃. Cumulative water temperatures to reach 8-cell stage, 50%-epiboly stage, optic vesicle formation stage, hearth formation stage, and hatching stage were 72.9, 356.7, 525.8, 735.2, and 1,156.7℃, respectively. Water temperature affected significantly the hatching rate (p< 0.001). The hatching rate decreased with increase of water temperature. The highest hatching rate was 62.0% at 7℃. When the water temperature was higher than 16℃, the proportion of abnormal larvae increased (p < 0.05). Salinity also affected significantly the hatching rate (p< 0.001). The highest hatching rate was 54.1% at the salinity (33.6 psu) of ambient seawater. When the salinity was lower than 30 psu, the proportion of abnormal larvae increased ip < 0.05).

Daily Mean Temperature and Urolithiasis Presentation in Six Cities in Korea: Time-Series Analysis

Chi, Byung Hoon,Chang, In Ho,Choi, Se Young,Suh, Dong Churl,Chang, Chong won,Choi, Yun Jung,Lee, Seo Yeon The Korean Academy of Medical Sciences 2017 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.32 No.6

<P>Seasonal variation in urinary stone presentation is well described in the literature. However, previous studies have some limitations. To explore overall cumulative exposure-response and the heterogeneity in the relationships between daily meteorological factors and urolithiasis incidence in 6 major Korean cities, we analyzed data on 687,833 urolithiasis patients from 2009 to 2013 for 6 large cities in Korea: Seoul, Incheon, Daejeon, Gwangju, Daegu, and Busan. Using a time-series design and distributing lag nonlinear methods, we estimated the relative risk (RR) of mean daily urolithiasis incidence (MDUI) associated with mean daily meteorological factors, including the cumulative RR for a 20-day period. The estimated location-specific associations were then pooled using multivariate meta-regression models. A positive association was confirmed between MDUI and mean daily temperature (MDT), and a negative association was shown between MDUI and mean daily relative humidity (MDRH) in all cities. The lag effect was within 5 days. The multivariate Cochran Q test for heterogeneity at MDT was 12.35 (<I>P</I> = 0.136), and the related I<SUP>2</SUP> statistic accounted for 35.2% of the variability. Additionally, the Cochran Q test for heterogeneity and I<SUP>2</SUP> statistic at MDHR were 26.73 (<I>P</I> value = 0.148) and 24.7% of variability in the total group. Association was confirmed between daily temperature, relative humidity and urolithiasis incidence, and the differences in urolithiasis incidence might have been partially attributable to the different frequencies and the ranges in temperature and humidity between cities in Korea.</P>

P70S6K and Elf4E Dual Inhibition Is Essential to Control Bladder Tumor Growth and Progression in Orthotopic Mouse Non-muscle Invasive Bladder Tumor Model

Chi, Byung Hoon,Kim, Soon-Ja,Seo, Ho Kyung,Seo, Hye-Hyun,Lee, Sang-Jin,Kwon, Jong Kyou,Lee, Tae-Jin,Chang, In Ho The Korean Academy of Medical Sciences 2015 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.30 No.3

Low-Dose Unenhanced Computed Tomography with Iterative Reconstruction for Diagnosis of Ureter Stones

Chi, Byung Hoon,Chang, In Ho,Lee, Dong Hoon,Park, Sung Bin,Kim, Kyung Do,Moon, Young Tae,Hur, Taekyu Yonsei University, College of Medicine 2018 Yonsei medical journal Vol.59 No.3

<P><B>Purpose</B></P><P>To study the clinical application of low-dose unenhanced computed tomography with iterative reconstruction technique (LDCT-IR) on renal colic in the emergency department.</P><P><B>Materials and Methods</B></P><P>We conducted a prospective, single-blinded, randomized, and non-inferiority study. From March 2014 to August 2015, 112 patients with renal colic were included, and were randomized to either LDCT-IR (n=46) or standard-dose unenhanced CT (SDCT) (n=66) groups. The accuracy of urolithiasis diagnosis was the primary endpoint of this study. Radiation dose, size and location of the stone, hydronephrosis, other diseases except urolithiasis, and results of treatment were analyzed between the two groups.</P><P><B>Results</B></P><P>The average effective dose radiation of SDCT was approximately four times higher than that of LDCT-IR (6.52 mSv vs. 1.63 mSv, <I>p</I><0.001). There was no significant difference in the accuracy of ureteral stone diagnosis between the two groups (LDCT-IR group: 96.97% vs. SDCT group: 98.96%, <I>p</I>=0.392). No significant difference was observed regarding the size and location of a stone, hydronephrosis, and diagnosis of other diseases, except urolithiasis. False negative results were found in two LDCT-IR patients and in one SDCT patient. In these patients, stones were misread as vascular calcification, and were difficult to diagnose because evidence of hydronephrosis and ureteral dilatation was not found.</P><P><B>Conclusion</B></P><P>LDCT-IR, as a first-line imaging test, was non-inferior to SDCT with respect to diagnosis of ureter stones, and was clinically available for the evaluation of renal colic.</P>

Dammarane계 Triterpenoid의 질량분광분석

한병훈(Byung Hoon Han),김제훈(Jae Hoon Kim),지형준(hyung Joon Chi) 한국생약학회 1979 생약학회지 Vol.10 No.2

Mass spectra of the dammarane triterpenes having open side chain and C₂₀-C_(25)-epoxy side chain were measured. Principal fragment ions were assigned and plausible mechanisms for the formation of the fragment ions were proposed. In general, the triterpenoids of C₂₀-C_(25)-epoxy side chain produce h₁-species fragment ions by the deletion of side chain at C₂₀-C₂₂ bond and open side chain triterpenoids produce h_2 species fiagmentions whose mass numbers are higher by two mass unit than those of h₁ species. The mass number of h species fragment ions will serve as the diagnostic tool for the elucidation of side chain structure of tetracyclic triterpenoids.