HaCaT 세포주에서 Epigallocatechin-3-Gallate (EGCG)가 자외선 및 LPS에 의한 iNOS mRNA 발현 및 NO 생성에 미치는 영향

탁우정 ( Tag U Jeong ),이창균 ( Lee Chang Gyun ),서성준 ( Seo Seong Jun ),김명남 ( Kim Myeong Nam ),노병인 ( No Byeong In ),홍창권 ( Hong Chang Gwon ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.1

N/A Background: Nitric oxide (NO) plays an important role in inflammation and multiple stages of carcinogenesis. Green tea (Camellia sinensis polyphenols are potent antiinflammatory agents and have been shown to inhibit NO production in tumor cell lines. In the present study, we examined the effect of epigallocatechinp-3-gallate (EGCG), a generation of NO in HaCaT cells. Methods: HaCaT cells were treated with 10μM EGCG and 100μM NAC for 1 hour. 1 hour later, they were irradiated with 50mJ/cm^2 UVB and treated with 200 u㎍/ml LPS for 12 hours, respectively. The iNOS mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR) and NO production was assessed by spectrophotometric method based on Griess reaction. Nuclear factor κB (NF-κB) binding activity was determined by electophoretic mobility shift assay (EMSA). Results: The results were as follows 1. EGCG inhibited UVB and LPS induced expression of inducible nitric oxide synthase. 2. HaCaT cells cotreated with EGCG produced significantly less iNOS mRNA and NO compared with HaCaT cells stimulated with UVB irradiation of LPS. 3. The inhibition of iNOS mRNA and NO production correlated with the suppression of expression of NF-κB dependent gene iNOS. 4. EGCG inhibited the activation and translocation of NF-κB to the nucleus Conclusion: inhibited the activation and translocation of NF-κB to the nucleus in HaCaT cells by interfering with the activation of NF-κB through a novel mechanism. Our results further suggest that EGCG may be therapeutically effective in UVB and cytokine induced cutaneous inflammation. (Korean J Dermatol 2004;42(1):37~46)

X-band Linac for a 6MeV dual-head radiation therapy gantry

Lee, Seung Hyun,Shin, Seung-Wook,Lee, Jongchul,Kim, Hui-Su,Lee, Byeong-No,Lee, Byung-Chul,Park, Hyung-dal,Song, Ki-back,Song, Ho-seung,Mun, Sangchul,Ha, Donghyup,Chai, Jong-Seo Elsevier 2017 Nuclear Instruments & Methods in Physics Research. Vol.852 No.-

<P><B>Abstract</B></P> <P>We developed a design for a 6MeV X-band linear accelerator for radiation therapy in a dual-head gantry layout. The dual-head gantry has two linacs that can be operated independently. Each X-band linac accelerates electron bunches using high-power RF and generates X-rays for radiation therapy. It requires a versatile RF system and pulse sequence to accomplish various radiation therapy procedures.</P> <P>The RF system consists of 9.3GHz, 2MW X-band magnetron and associated RF transmission components. A test linac was assembled and operated to characterize its RF performance without beam. This paper presents these results along with a description of the gantry linacs and their operational requirements.</P>

Assessment of Magnetic Field Mitigation and Electrical Environmental Effects for Commercially Operating 154kV Transmission Lines with Passive Loop

Lee, Byeong-Yoon,Myung, Sung-Ho,Ju, Mun-No,Cho, Yeun-Gyu,Lee, Dong-Il,Lim, Yun-Seog,Kim, Sang-Beom The Korean Institute of Electrical Engineers 2014 Journal of Electrical Engineering & Technology Vol.9 No.3

Power frequency magnetic field is still a critical problem for new construction of overhead power transmission lines in Korea because most people have been concerned about possibly carcinogenic effects of it. Although reference level of power frequency(60Hz) magnetic field has been set to 200uT in ICNIRP guidelines published in 2010, Korean government has no intention of adjusting 83.3uT specified by law in 2006 to this new reference level in consideration of people's concerns for the time being. Regardless of the current regulated magnetic field value, electric utility company has been trying to reduce magnetic field in the residential area in the vicinity of overhead power transmission lines to take into account of public concerns on the long-term effect of magnetic fields. In an effort to reduce magnetic field, engineering side has made considerable efforts to develop passive loop based, cost-effective mitigation technique of power frequency magnetic field more than ten years. In order to verify developed power frequency magnetic field mitigation technique based on passive loop, a horizontal type of passive loop was designed and installed for commercially operating 154kV overhead power transmission line for the first time in Korea. The measurement results before and after the installation of passive loop showed that magnetic field could be reduced to about 20%. The electrical environmental effects such as AN, RI and TVI were assessed before and after the installation of passive loop and these values were complied with the requirements specified by electric utility. It has been confirmed from the field test results that passive loop could be commercially and cost-effectively utilized to mitigate power frequency magnetic field.

Assessment of Magnetic Field Mitigation and Electrical Environmental Effects for Commercially Operating 154kV Transmission Lines with Passive Loop

Byeong-Yoon Lee,Sung-Ho Myung,Mun-No Ju,Yeun-Gyu Cho,Dong-Il Lee,Yun-Seog Lim,Sang-Beom Kim 대한전기학회 2014 Journal of Electrical Engineering & Technology Vol.9 No.3

Power frequency magnetic field is still a critical problem for new construction of overhead power transmission lines in Korea because most people have been concerned about possibly carcinogenic effects of it. Although reference level of power frequency(60Hz) magnetic field has been set to 200uT in ICNIRP guidelines published in 2010, Korean government has no intention of adjusting 83.3uT specified by law in 2006 to this new reference level in consideration of people’s concerns for the time being. Regardless of the current regulated magnetic field value, electric utility company has been trying to reduce magnetic field in the residential area in the vicinity of overhead power transmission lines to take into account of public concerns on the long-term effect of magnetic fields. In an effort to reduce magnetic field, engineering side has made considerable efforts to develop passive loop based, cost-effective mitigation technique of power frequency magnetic field more than ten years. In order to verify developed power frequency magnetic field mitigation technique based on passive loop, a horizontal type of passive loop was designed and installed for commercially operating 154kV overhead power transmission line for the first time in Korea. The measurement results before and after the installation of passive loop showed that magnetic field could be reduced to about 20%. The electrical environmental effects such as AN, RI and TVI were assessed before and after the installation of passive loop and these values were complied with the requirements specified by electric utility. It has been confirmed from the field test results that passive loop could be commercially and cost-effectively utilized to mitigate power frequency magnetic field.

실업계 고교와 전문대학간 연계 교육과정 평가

이병욱(Lee Byeong Ug),이상혁(Lee Sang Hyeog),노태천(No Tae Cheon) 대한공업교육학회 2003 대한공업교육학회지 Vol.28 No.2

We have several same lessons for vocational high school, junior college. So the students who graduate vocational high school are studying several same lessons in junior college. Therefore many vocational high school graduates lose their interest in major and can not cultivate the creative thinking and high level vocational ability. For that reason the purpose of this study is to grope for concrete plan to relate the curriculums between vocational high school and junior college through the evaluation of the articulation curriculum. This study is completed by analyzing several documents and materials which are connected with articulated education, and by checking up questionnaire for vocational high school teachers and junior college professors through the actual evaluation of articulated curriculum implementation. The measures obtained from this study were as follows: 1. The importance of articulated curriculums in vocational schools will be increase, and articulated curriculum, based on National skill standards(NSS), should be established and managed to meet the speed and content of industrial change. 2. The vocational schools curriculum should not be connected simply by relating textbook, class, and human material reassures but by articulating systematically and essentially. 3. The concrete articulated co-operation plan in vocational high school and junior college curriculum implementation is like followings. ① Make systematic articulation in subject contents of the same course. ② Make financial support for articulated education and establishing policy for raising accountability in vocational high school. ③ Make up for the rules to admit the major subjects in vocational high school as credits in junior college. ④ Make articulated plans for special activities such as club activity, starting an enterprise activity(BizCool; Business School), inventional education, etc. ⑤ Make a evaluation team to revise and correct the quality of articulated curriculum and assess it.

Optically isotropic liquid crystal mixtures and their application to high-performance liquid crystal devices

Park, No Hyun,Noh, Seong Cheol,Nayek, Prasenjit,Lee, Myong-Hoon,Kim, Min Su,Chien, Liang-Chy,Lee, Joun Hoo,Kim, Byeong Koo,Lee, Seung Hee Taylor Francis 2015 Liquid crystals Vol.42 No.4

심부정맥혈전증의 병인 분석

이제환 ( Lee Je Hwan ),박선양 ( Park Seon Yang ),계경채 ( Gye Gyeong Chae ),정철원 ( Jeong Cheol Won ),신현춘 ( Sin Hyeon Chun ),이진학 ( Lee Jin Hag ),양성현 ( Yang Seong Hyeon ),김병국 ( Kim Byeong Gug ),김노경 ( Kim No Gyeon 대한내과학회 1993 대한내과학회지 Vol.44 No.3

연구배경 : 심부정맥혈전증은 체내 지혈혈전계의 불균형에 의해 발생하며 최근 혈전증 연구의 발달로 심부정맥혈전증의 여러 가지 선행 인자들이 알려져 있다. 국내에서도 상당수의 심부정맥혈전증이 발생하고 있으나 그임상상 및 발생요인 등의 병인론에 관해서는 잘 알려져 있지 않다. 이에 저자들은 서울대학교병원 내과에서 심부정맥혈전증(종양색전은 제외)으로 진단받은 85예의 환자를 대상으로 임상적 선행 인자를 조사하였으며 동시에 혈액응고학적 선행 인자에 대한 검사를 실시하였다. 방법 : 혈전증의 임상상 및 혈전증의 임상적 선행 인자를 알기 위하여 대상 환자들의 병록지와 심부정맥혈전증의 진단시 시행된 방사선학적 검사를 검토하였으며, 혈전증을 유발할 수 있는 혈액응고학적 선행인자의 분석을 위하여는 항 cardiolipin 항체, C 단백, S 단백, antithtombin Ⅲ, tissue-type plasminogen activator 및 plasminogen activator inhibitor type 1, 혈장 섬유소 용해능을 검사하였다. 결과 : 1) 대상 환자 85예중 남자 40예, 여자 45예로 남녀비는 1 : 1.13 이었고, 나이는 15세에서 77세로 중앙치는 47세였다. 대상 환자의 연령별 분포는 40대가 21예 (24.7%)로 가장 많았고, 50대가 19예(22.4%), 30대가 16예 (18.8%), 20대가 10예(11.7%), 60대가 9예 (10.6%), 70세 이상이 6예(7.1%)였고, 20세 미만도 4예 (4.7%) 있었다. 2) 심부정맥 혈전증의 발생 부위로는 장단지(calf)의 정맥에 국한된 경우가 7예(8.2%), 근위부쪽으로 슬와정맥(popliteal vein)까지 관여된 경우가 7예(8.2%), 장골 정맥(femoral vein)까지 관여된 경우가 12예 (14.1%), 대퇴 정맥(iliac vein)까지 관여된 경우가 37예(43.5%)로 하지가 전체 대상 환자의 74.1%인 63예였으며, 하대 정맥과 신 정맥이 관여된 경우가 8예(9.4%), 문 맥(portal vein)이 관여된 경우가 8예(9.4%)로 복강내 정맥 혈전증이 16예(18.8%) 있었다. 그외 상지의 정맥이 관여된 경우가 4예(4.7%), 상대 정맥 혈전증이 1예(1.2%)있었으며, 심부정맥혈전증의 증거가 없는 폐동맥 색전증이 1예(1.2%)있었다. 전체 대상 환자중 29예(34.1%)에서 폐동맥 색전증이 합병하였다. 3) 심부정맥혈전증의 임상적 선행 인자로는 고령(60세 이상)이 15예(17.0%), 악성종양 13예(15.3%), 혈전증의 과거력이 있는 경우가 11예(12.9%), 수술후 상태가 9예(10.6%), immobilization이 7예(8.2%), 고지질혈증 5예(5.9%), 비만증 4예(4.7%), 혈류의 정체 4예(4.7%), 신증후군 3예(3.5%), 당뇨병 3예(3.5%), Behcet씨 병 2예(2.4%), 여성호르몬제 복용 2예(2.4%) 등이었으며, 특별한 임상적 위험인자가 발견되지 않은 경우가 25예(29.4%)였다. 4) 심부정맥혈전증의 혈액응고학적 선행 인자로는 항 cardiolipin 항체가 양성인 경우, C 단백의 기능적 활성도나 유리형 S 단백의 항원량이 정상의 60% 미만인 경우, antithrombin Ⅲ의 기능적 활성도가 정상의 70%미만인 경우, t-PA값이 정상치보다 낮거나 PAI-1 값이 정상치보다 높은 경우, 그리고 섬유소 용해능이 정상치 보다 낮은 경우 등을 생각하였는데, 항 cardiolipin 항체양성이 7예(19.4%), C 단백의 기능적 활성도가 정상의 60% 미만인 경우가 3예(16.7%), S단백(free form)의 항원량이 정상의 60% 미만인 경우가 3예(10.7%), antithtombin Ⅲ의 기능적 활성도가 정상의 70% 미만인 경우가 2예(5.9%), t-PA가 정상치보다 낮은 경우가 5예(22.7%), PAI-1이 정상치보다 높은 경우가 10예(29.4%), 섬유소 용해능 검사가 정상치보다 낮게 나온 경우가 14예(42.4%) 있었다. 결론 : 한국인에서의 심부정맥혈전증의 중요한 임상적 선행원인으로는 고령, 악성종양, 혈전증의 과거력, 수술후 상태, 기동장애였고, 혈전증과 관련된 혈액응고학적 검사의 이상소견으로는 PAI-1의 증가와 t-PA의 감소, 항 cardiolipin 항체 양성이 흔히 관찰되었다. Background : The formation of deep vein thrombosis reflects a balance between the effects of thrombogenic stimuli and a series of protective mechanisms. Substantial progress has been made in the last several decades in identifying hereditary and acquired risk factors predisposing to deep vein trombosis. Even so, a large number of patients still have no identifiable underlying cause for recurrent venous thrombosis. Elucidation of specific predisposing factor (s) is required for proper management of thrombosis. For the Korean patients, these factors have not been well characterized. Methods : We analyzed clinical profiles of the patients with venous thromboembolism and investigated the laboratory abnormalities known to be associated with increased risk of thrombosis. Results : 1) The male-female ratio was 1 : 1. 13 and age distribution showed 24.7% in fifth decade, 22.4% in sixth, 18.8% in fourth, 11.7% in third, 10.6% in seventh, 7.1% over 70 years old, and 4.7% under 20 years. 2) The thromboses were most commonly located in lower extremities (74.1%), and intraabdominal thromboses were 16 cases (18.8%), thromboses of upper extremities 4 cases (4.7%), superior vena cave thrombosis 1 case (1.2%) and pulmonary embolism without evidence of deep vein thrombosis 1 case (1.2%). Thirty-four percent of the cases were diagnosed as having pulmonary embolism. 3) The clinical risk factors for venous thromboembolism were old age(17.0%), malignancy (15.3%), prior history of venous thromboembolism (12.9%), postoperative state (10.6%), immobilization (8.2%), hyperlipidemia (5.9%), systemic lupus erythemato년 (4.7%), obesity (4.7%), stasis (4.7%), nephrotic syndrome (3.5%), diabetes mellitus (3.5%), Behcet`s disease (2.4%), estrogen (2.4%). Twenty-nine percent of the cases had no indentifiable clinical risk factors. 4) The laboratory abnormalities associated with venous thromboembolism were increase of anticardiolipin antibody (19.4%), decrese of protein C activity (16.7%), decrease of protein S (free form) antigenicity (10.7%), decrease of antithrombin Ⅲ activity (5.9%), decrease of tissue-type plasminogen activator (t-PA) (22.7%), increase of plasminogen activator inhibitor type 1 (PAI-1) (29.4%) and decrease of fibrinolytic activity (42.4%). Conclusion : Clinical and laboratory risk factors have been determined in 85 patients with deep vein thrombosis and/or pulmonary embolism in Korea. Major clinical risk factors for venous thromboembolism included old age, malignancy, prior history, postoperative state and immobilization. Among the laboratory abnormalities associated with venous thromboembolism, increase of PAI-1 and/of decrease of t-PA, and increase of anticardiolipin antibody were most frequently observed.

유제품의 안정적인 공급을 위한 젖소건강관리 시스템

이승빈(Seung-bin Lee),이해성(Hae-seong Lee),정노권(No-Kwon Jeong),전병규(Byeong-kyu Jeon),이상문(Sang-moon Lee) 한국컴퓨터정보학회 2015 한국컴퓨터정보학회 학술발표논문집 Vol.23 No.1

최근 국내 유제품 수요는 인구의 고령화, 학교급식 소비의 감소, 대체음료 시장의 성장 그리고 출산율 저하라는 여러 가지 영향에 기인하여 줄고 있다. 반대로 수입 유제품의 수요, 특히 치즈에 있어서는 눈에 띄는 성장을 보여 국내 낙농업 시장은 안타까운 실정이다. 뿐만 아니라 공급에 있어서는 계절적인 원유 수급불안 현상, 수입증가, 농가이탈에 의한 생산기반의 위축으로 인해 안정적으로 국내 낙농업 시장의 활성화가 필요한 시점이다. 이 논문에서는 낙농가에서 가장 중요한 젖소의 건강 관리(발정기, 맥박감지)를 통합해 유량을 늘리는 방안을 제시하여 낙농업계의 활성화를 목표로 한다.

현장실증기반 데이터를 활용한 유형별 마이크로그리드 상호통합형 비즈니스 모델에 관한 연구

이서형(Seo-hyeong Lee),노일래(Il-rae No),이일무(Il-moo Lee),이재윤(Jeayou Lee),김홍기(Honggi Kim),김병만(Byeong-man Kim) 대한전기학회 2022 대한전기학회 학술대회 논문집 Vol.2022 No.5

식도운동질환과 위식도역류질환과의 연관성

홍성노 ( Hong Seong No ),이풍렬 ( Lee Pung Lyeol ),이준행 ( Lee Jun Haeng ),김영호 ( Kim Yeong Ho ),김재준 ( Kim Jae Jun ),백승운 ( Baeg Seung Un ),유병철 ( Yu Byeong Cheol ),이종철 ( Lee Jong Cheol ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<목적> 식도 운동의 장애는 위식도역류의 발생과 연관이 있으며, 식도운동질환과 위식도역류질환과의 연관성을 시사하는 여러 연구가 보고되고 있다. 이 중 새로이 분류된 비효과적식도운동(ineffective esophageal motility)은 위식도역류질환의 주요 식도내압검사 소견으로, 일부 연구에서는 위식도역류질환의 표지(marker)라고 주장되기도 하지만 논란이 되고 있으며, 이를 제외한 다른 식도운동질환에서 위식도역류질환의 빈도와 연관성을 살펴본 연