Chitinase-producing Salinivibrio bacteria isolated from salt-fermented shrimp with antimicrobial and safety assessments

( Bao Le ),( Gyuhwa Chung ),( Seung Hwan Yang ) 한국응용생명화학회(구 한국농화학회) 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3

Chitinases are glycosyl hydrolases which cleave the β- 1,4 linkage of chitin into oligo or monomers of N-acetylglucosamine. These bacterial enzymes have been used for a wide range of applications in the food and pharmaceutical industries. In this study, we isolated two potential chitinolytic strains, BAO-01 and BAO-02, from salt-fermented shrimp, which were shown to belong to the genus Salinivibrio through genetic characterization using 16S rRNA. These isolates were gram-positive, rod-shaped, and non-spore forming. BAO-01 showed greater growth and chitinase activity than BAO-02 after the incubation at 37 oC for 4 days. Both strains grew on a wide range of carbon and nitrogen sources, pH values, temperatures, and salt levels. However, they showed minor biochemical differences. In addition, their antimicrobial activities against foodborne pathogens and antibiotic susceptibilities were evaluated. These Salinivibrio spp. did not show bioamine production, hemolytic activity, and mucin degradation. Therefore, the in vitro screening results suggested that these bacteria could be widely used as new candidates for chitin hydrolyzation and seafood fermentation.

Crystal facet engineering induced anisotropic transport of charge carriers in a perovskite

Yang, Hewei,Zhou, Yunzhan,Yang, Yijun,Yi, Ding,Ye, Tao,Lam, Tran Dai,Golberg, Dmitri,Bao, Bate,Yao, Jiannian,Wang, Xi The Royal Society of Chemistry 2018 Journal of Materials Chemistry C Vol.6 No.43

<P>Precise control of crystal orientations and macroscopic morphology of a perovskite crystal is crucial for various optoelectronic applications relying on charge carrier transport tuning along exposed crystal facets. Here, taking methylammonium lead bromide (CH3NH3PbBr3) as an example, and employing a novel crystal facet engineering method, we successfully construct two kinds of perovskite crystals with exposed {001} and {110} facets. We find that the free carriers’ photoluminescence lifetime on the {001} facets can be 3 times longer than that on {110} facets. The related mechanisms are investigated <I>via</I> fluorescence lifetime imaging microscopy and <I>in situ</I> transmission electron microscopy. These indicate that the different trap state density of exposed facets and crystal structure changing of CH3NH3PbBr3 under light and electron beam irradiation lead to the differences in carrier transport along different facets. By distinguishing the charge carrier transport on different CH3NH3PbBr3 exposed facets, micro-photodetectors have been constructed. A device fabricated with the {001} exposed facets exhibited two orders of magnitude higher photocurrent and half as much dark current as a {110} facet-based device. Thus, the crystal facet engineering of perovskites can be widely adopted for understanding physical/chemical properties of perovskite crystals and provides great potential for novel perovskite optoelectronic device applications.</P>

Dynamic Analysis of Time Series Data Based on State Space Model

Yang Zhizhong,Xi Bao 보안공학연구지원센터 2014 International Journal of u- and e- Service, Scienc Vol.7 No.5

The state space model is effective on analyzing non-stationary time series data, especially in adapting better to the dynamic variation analysis of the time series data and forecasting demand,by replacing fixed parameters with the variable ones.This article elaborates the constructing process of state space model by the measurement equation and state equation. This article also selects M0 money supply, M1 money supply, M2 money supply as the characterize variables of monetary policy, selects the national housing climate Index as characterize variables of real estate development,status regression model with stronger dynamic analysis capabilities as empirical analysis tool, with 2005 to 2012 monthly data of relevant variables as empirical analysis object, carry out the empirical study of relationship between the development of China's real estate industry and the amount of the three currencies. The empirical results show that the amount of three currencies elastic influence for real estate development are positive, M2 money supply impact of greater intensity. Among, M0 money supply influence gradually weakened, M1 and M2 money supply influence gradually increased.

Effect of Organizational Virtuousness on Pro-social Rule Breaking: Mediating Role of Psychological Empowerment and Moderating Role of Proactive Personality

Yang, Mao-Bao(양무보),Chung, Soo-Jin(정수진) 한국산업경제학회 2020 산업경제연구 Vol.33 No.3

본 연구의 목적은 조직 미덕이 친사회적 규칙위반행동에 미치는 영향, 심리적 임파워먼트의 매개효과 및 주도적 성격의 조절효과를 검증하는 것이다. 본 연구는 이론적 연구를 바탕으로 세 가지 주요한 연구 가설을 제시하고 연구 모델을 수립하였다. 데이터는 설문조사 방법에 의해 중국 내 직원으로부터 수집되었고, SPSS 24.0은 연구 모델의 가설을 검증하기 위해 사용되었다. 조직 미덕은 친사회적 규칙위반 행동에 긍정적인 영향을 미치고, 심리적 임파워먼트는 두 변수의 관계에 매개효과를 미치며, 주도적 성격은 두 변수의 관계에 조절효과를 미친다는 연구 결과가 나왔다. 본 연구는 기업의 미덕과 윤리의 관점에서 기업의 경제적 효율성을 어떻게 향상시킬 것인가에 대한 새로운 방식을 제공하며, 관리자는 직원들의 압박 수용력을 증대시키고 기업 효율성을 향상시키기 위해 자신의 기업 미덕 형성을 중요시하고 미덕적인 근무 환경을 조성하는 데 관심을 기울여야 한다. The aim of this study is to verify the impact of Organizational Virtuousness on Pro-social Rule Breaking, the mediating effect of Psychological Empowerment and the moderating effect of Proactive Personality. Based on theoretical research, this study was established a research model by proposes 3 key research hypotheses. Data were used to test the hypotheses of the research model by SPSS 24.0, which were collected from employees in China by the survey method. Organizational Virtuousness is positively related to Pro-social Rule Breaking, Psychological Empowerment as the mediating variable and Proactive Personality as the moderating variable effect Organizational Virtuousness and Pro-social Rule Breaking. This study provides a new way of thinking about how to improve the economic efficiency of the enterprise from the perspective of corporate virtuousness and ethics. Managers should pay attention to the construction of their own corporate virtuousness and create a virtuous working atmosphere in order to enhance staff"s strain capacity and improve corporate efficiency.

Biomechanical Evaluation of 2 Endoscopic Spine Surgery Methods for Treating Lumbar Disc Herniation: A Finite Element Study

Yang Zou,Shuo Ji,Hui Wen Yang,Tao Ma,Yue Kun Fang,Zhi Cheng Wang,Miao Miao Liu,Ping Hui Zhou,Zheng Qi Bao,Chang Chun Zhang,Yu Chen Ye 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods: Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results: In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion: In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.

Fabrication and characterization of porous poly(lactic-co-glycolic acid) (PLGA) microspheres for use as a drug delivery system

Bao, Trinh-Quang,Hiep, Nguyen-Thi,Kim, Yang-Hee,Yang, Hun-Mo,Lee, Byong-Taek Springer-Verlag 2011 JOURNAL OF MATERIALS SCIENCE - Vol.46 No.8

Voice Activity Detection Algorithm based on Improved Radial Basis Function Neural Network

Bao-yuan Chen,Ya-qiong Lan,Jing-yang Liu,Zi-he Li,Xiao-yang Yu 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

Voice activity detection (VAD) is the key of voice recognition, voice synthesis and speech-sound enhancement.For the sake of improve the accuracy and robustness of speech endpoint detection system. Combining the advantages of adaptive genetic algorithm (AGA) and improved radial basis function network (RBF) defects in existing learning methods. This paper presents a comprehensive detection method-- Adaptive genetic algorithm radial basis function network. This method uses adaptive genetic algorithm to simultaneously optimize the center, the width and the structure of RBF network. The method using wavelet analysis to extract the characteristics of the speech signal, use them as an input amount to the radial basis function networks. Establish voice detection system model, this method enhance the accuracy of the detection system and has better robustness.

A circuit mechanism of time-to-space conversion for perception

Yang, Sunggu,Chung, Jaeyong,Jin, Sung Hun,Bao, Shaowen,Yang, Sungchil Elsevier 2018 Hearing research Vol.366 No.-

<P>Sensory information in a temporal sequence is processed as a collective unit by the nervous system. The cellular mechanisms underlying how sequential inputs are incorporated into the brain has emerged as an important subject in neuroscience. Here, we hypothesize that information-bearing (IB) signals can be entrained and amplified by a clock signal, allowing them to efficiently propagate along in a feedforward circuit. IB signals can remain latent on individual dendrites of the receiving neurons until they are read out by an oscillatory clock signal. In such a way, the IB signals pass through the next neurons along a linear chain. This hypothesis identifies a cellular process of time-to-space and sound-to-map conversion in primary auditory cortex, providing insight into a mechanistic principle underlying the representation and memory of temporal sequences of information. (C) 2018 Elsevier B.V. All rights reserved.</P>

Self-care Experiences of Adolescents with Spinal Muscular Atrophy

Bao-Huan Yang,Chia-Ying Chung,Wen-Chin Weng,Kao-Wen Lo,Yuh-Shiow Li 한국간호과학회 2021 Asian Nursing Research Vol.15 No.4

Purpose: We examined the self-care experiences of adolescents with spinal muscle atrophy (SMA) and their perceptions of the interactions between their body and the environment. Methods: We interviewed ten adolescents with SMA aged 13-18 years regarding personal care practices. Purposive sampling was conducted in two medical centers in northern Taiwan. Data were analyzed using the Giorgi analysis method. Results: Four constitutions were identified: (1) limited space for independent development, (2) multiple reconstructions of self-image to improve physical ability, (3) self-care of disease, and (4) developing activity styles to accommodate social culture. Conclusion: The self-care lived experiences of patients reflect dynamic changes in the body and environment. Self-existence was exhibited by adjustment, practice, and creativity of physical activity to integrate into society. Nursing staff should understand the self-care experiences and needs of adolescents with SMA to develop a database of self-care skills. This study recommended that nursing staff improve their ability to guide patients in taking care of themselves by developing body awareness self-care courses and individual care plans in response to various stages of disability to help patients delay deterioration, realize their physical potential, and promote independence and social development.

Partnership Between Families of Children with Muscular Dystrophy and Health-care Professionals: From Parents' Perspective

Bao-Huan Yang,Chia-Ying Chung,Yuh-Shiow Li 한국간호과학회 2018 Asian Nursing Research Vol.12 No.2

Purpose: At present, there is still controversy between parents of children with muscular dystrophy (MD) and health-care professionals on care issues. Partnerships can connect the affected children and their families to appropriate health-care services, to jointly face the care environment together and thereby improve the quality of life of children with MD. Therefore, the objective of this study is to explore partnerships between families and health-care professionals from the perspectives of parents of children with MD. Methods: Husserl's phenomenological research was applied to explore the basic structures of parents' descriptions of MD. Through purposive sampling, we conducted in-depth interviews with parents, and analyzed the data according to the theory of Giorgi. Nineteen parents (10 mothers, 9 fathers) participated in this study. The precision of the research results was tested by applying the four standards of Lincoln and Guba. Results: This study identified five constituents: feasible resources and detailed care information; the provision of an integrated medical care across systems; family and home as key elements in critical care; respect and care for family care demands; and finally, feedback and support from families. Conclusion: This study demonstrated that partnerships were established by health-care professionals, enhancing the care capacity of the families, developing the preventive medicine of MD, and enhancing children's potential for self-care within the families. Hospital policies should include the promotion of family partnership care. The findings can help health-care professionals recognize the life experiences of children with MD when providing medical care.