갑상선질환의 세침흡인도말검사에서 질환별 특징적인 소견에관한 연구: 유두상 암종과 다른 질환과의 비교를 중심으로

백운철 ( Oun Chul Back ),김성철 ( Sung Chul Kim ),김태근 ( Tae Geun Kim ),유숙 ( Suk Yu ),노정환 ( Joung Whan Ro ),김태전 ( Tai Jeon Kim ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

This study observed appearance existence and non-existence of nuclear groove, intranuclear pseudoinclusion, papi1lae, psammoma body, lymphocyte, multinucleated giant histiocyte and follicular structure that is a feature of cytologic change in FNAC of several thyroid nodules, and analyzed cytologic features of other thyroid diseases in comparison with papillary carcinoma of the thyroid. The FNAC total in 103cases(Benign lesion 40cases, Neoplasm 22cases and Malignant lesion 41cases) through a Hematoxylin-eosin and Papanicolaou stain from cytomorphologic change by nodular lesions was observed and compared. The results were as follows. A papillary structure(88%) and psammoma body(24%) were observed in papi1lary carcinoma, and intranuclear pseudoinclusion was observed in papi1lary carcinoma(94%) and medullary carcinoma(29%), and nuclear grooves were observed in all nodular lesions(table 5). Appearance of lymphocytes was observed in all nodular lesions except hurthle cell tumor but appearance frequency was highly remarkable in grave’s disease(l 00%), hashimoto’s thyroiditis(1 00%) and subacute thyroiditis( 100%). A multinucleated giant histiocyte was observed in subacute thyroiditis(loo%), papillary carcinoma(82%), adenomatous goiter(33%) and hashimoto’s thyroiditis(26% ) but expressed a high appearance frequency in subacute thyroiditis(Table 5). A follicular structure was observed in all nodular lesions except hurthle cell tumor and sub acute thyroiditis but expressed a high appearance frequency in follicular neoplasm(loo%) and grave’s disease(loo%) (Table 5). Conclusively, a papillary structure and psammoma body of cytologic features change showed papi1lary carcinoma in FNAC but it is considered when diagnosing papillary carcinoma because nuclear grooves and intranuclear pseudoinclusion can be observed in other nodular lesions.

골조직의 신속한 진단을 위한 탈회방법의 비교 연구

김성철 ( Sung Chul Kim ),백운철 ( Oun Chul Back ),김태전 ( Tai Jeon Kim ),배형준 ( Hyung Joon Bae ),강희규 ( Hee Gyoo Kang ) 대한임상검사과학회 2005 대한임상검사과학회지(KJCLS) Vol.37 No.1

These studies were done to know decalcification methods to reduce the time of decalcification for quick bone tissue diagnosis. When bone tissue was decalcified with 10 % formic acid at room temperature, decalcification and hematoxylin & eosin (H&E) stains were complete and satisfactory after 12 hours, but some of the tissue sections fell off during staining. In this way, decalcification, H&E stains were complete and satisfactory after 24 hours, 36 hours and 48 hours, tissue sections didn``t fall off during staining. When bone tissue was decalcified with 10 % formic acid in a 60 ℃ paraffin oven, decalcification and H&E stains were complete and satisfactory after 6 hours, but some tissue sections fell off during staining. In this way, decalcification and tissue sections were complete, with no falling off during staining after 8 hours, 10 hours, 12 hours, 14 hours, 24 hours, or H&E stains were satisfactory from 8 hours to 12 hours, but H&E stains appeared to reddish nucleus after 14 hours and 24 hours. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at DECAL machine frequencies of 15 Hz and 45 Hz, and for 6 hours, 12 hours and 24 hours at a DECAL machine frequency of 90 Hz. Decalcification and H&E stains were complete and satisfactory after 6 hours at the 15 Hz and 45 Hz DECAL settings. Some of the tissue sections fell off during staining at the 15 Hz DECAL machine setting. At the 90 Hz setting, decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 4 hours. In this way, decalcification, H&E stains, and tissue section were complete and satisfactory with no falling off during staining after 12 hours, 24 hours at all machine settings. Bone tissue was decalcified with 10 % formic acid for 6 hours, 12 hours and 24 hours at 37 ℃ 3 hours, 6 hours and 12 hours at 45 ℃ and 1 hours, 5 hours and 10 hours at 60 ℃ with the RHS-1 machine setting at 60Hz. At the temperatures of 37 ℃, 45 ℃, and 60 ℃ decalcification, H&E stains, and tissue sections were complete and satisfactory, with no falling off during staining except for after 6 hours at 37 ℃. 3 hours, 1 hours, or decalcification, H&E stains, and tissue sections were complete and satisfactory with no falling off during staining after 12 hours and 24 hours at 37 ℃, 6 hours and 12 hours at 45 ℃, and 5 hours at 60 ℃. But H&E stains appeared to reddish nucleus after 10 hours at 60 ℃. From the above reults, the authors were able to deduce that decalcification is accelerated by heat and frequency. We therefore think that it is necessary for machines which are similar to the RHS-1 machine to be maintained at the temperature evenly with agitation effect for quick decalcification.

H. pylori 검출을 위한 CLO 검사, Geimsa 염색 및 면역조직화학 염색의 비교 분석

유숙 ( Suk Yu ),백운철 ( Oun Chul Back ),김종열 ( Jong Yull Kim ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

In order to detect a rational method for reducing the ratios of the false positive and the false negative appearing from the CLO test and giemsa staining used for the examination of H. pylori, we have compared with the results of CLO test and giemsa staining of which the false positive and the false negative are shown through this study. We have divided the groups as following four groups: A, B, C, and D group. And as for the respective group we have executed the immunohistochemical staining reported as the highest in terms of sensitiveness and particularity and have got to the following results through the reciprocal comparisons of H. pylori detection results. 1. A Group; CLO test positive and giemsa staining positive As a result, immunohistochemical staining showed a higher degree rather than giemsa staining in terms of sensitiveness. 2. B Group; CLO test negative and giemsa staining positive As a result, false positive of giemsa staining against the results of the negative of immunohistochemical staining was elaborated as stained in the mucus and the similar bacteria. And the false negative of CLO test that does not coincide with the results of immunohistochemical staining was elaborated as inadequacy of the option of the organic extraction. In such a way, immunohistochemical staining showed a higher degree rather than CLO test in terms of the sensitiveness and particularity. 3. C Group; CLO test positive and Giemsa staining negative As a result, false negative was judged as stained bacteria as the number of H. pylori is not so many or colored in blue color in the stain background in the giemsa staining. And the false positive of CLO test was elaborated as polluted from bacteria in the inside of the oral cavity at the time of organic extraction. 4. D Group; CLO test negative and giemsa staining negative Immunohistochemical staining showed negative in all for cases, and coincided with the results of CLO test and giemsa staining. In conclusion, immunohistochemical staining was made in only H. pylori through antigen and antibody. CLO test and giemsa staining showed positive and negative in gastric mucosa. Therefore, as for the detection body of which the results of CLO test and giemsa staining does not coincide like immunohistochemical staining, to let reconfirm through the examination method of high sensitiveness and high characteristics would be of help for an exact detection of H. pylori and an earlier treatment.

유방암의 악성도에 따른 스테로이드호르몬 수용체와 종양단백질의 발현과의 상관성

김태전 ( Tai Jeon Kim ),문희주 ( Hi Joo Moon ),배형준 ( Hyung Joon Bae ),백운철 ( Oun Chul Back ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.1

This study was designed to compare and analyze the overall relationship between independent factors inducing breast cancer, the steroid hormone receptors(ER and PR), and oncoproteins(C-erbB-2, P-53 and Bcl-2). A total of 21 test samples underwent tissue studies with H&E staining to test histologic grading. ER, PR, P-53, C-erbB-2, Bcl-2, and Ki 67 were examined with immunohistochemistry. Although ER and PR showed no statistically significant difference in histologic grade, the induction of PR increased when induction of ER increased showing a significant relationship(P<0.05). C-erbB-2 oncoprotein showed no statistically significant relationship with histologic grade, nuclei mitosis index, lymphnode metastasis, menopause, and P-53 oncoprotein, whereas it showed a statistically significant relationship with PR, ER, and Bcl-2 oncoproteins(P<0.05). P-53 oncoprotein showed no statistically significant relationship with C-erbB-2 oncoprotein, histologic grade, lymphnode metastasis, and menopause, whereas it showed statistically significant relationships with ER, PR, Bcl-2, and nuclei mitosis index(P<0.05). Bcl-2 oncoprotein showed no statistically significant relationship with lymphnode metastasis, histologic grade, and menopause, whereas it showed statistically significant relationships with other pathological prognosis factors(ER, PR, P-53, C-erbB-2 and nuclei mitosis index)(P<0.05). In conclusion, ER, PR, and oncoproteins(C-erbB-2, P-53 and Bcl-2) showed no significant difference with histologic grade. However ER, PR, and oncoproteins showed significant relationships with each other. The induction rate of P-53 oncoprotein was low when that of Bcl-2 oncoprotein was high while Bcl-2 oncoprotein and P-53 oncoprotein showed a reciprocal relationship with each other.

갑상선질환의 세침흡인도말검사에서 질환별 특징적인 소견에 관한연구 : 유두상 암종과 다른 질환과의 비교를 중심으로

백운철,김성철,김태근,유숙,노정환,김태전 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

This study observed appearance existence and non-existence of nuclear groove, intranuclear pseudoinclusion, papillae, psammoma body, lymphocyte, multinucleated giant histiocyte and follicular structure that is a feature of cytologic change in FNAC of several thyroid nodules, and analyzed cytologic features of other thyroid diseases in comparison with papillary carcinoma of the thyroid The FNAC total in 103cases(Benign lesion 40cases, Neoplasm 22cases and Malignant lesion 41cases) through a Hematoxylin-eosin and Papanicolaou stain from cytomorphologic change by nodular lesions was observed and compared. The results were as follows. A papillary structure(88%) and psammoma body(24%) were observed in papillary carcinoma, and intranuclear pseudoinclusion was observed in papillary carcinoma(94%) and medullary carcinoma(29%), and nuclear grooves were observed in all nodular lesions(table 5). Appearance of lymphocytes was observed in all nodular lesions except hurthle cell tumor but appearance frequency was highly remarkable in grave's disease(l00%), hashimoto's thyroiditis(100%) and subacute thyroiditis(l00%). A multinucleated giant histiocyte was observed in subacute thyroiditis(l00%), papillary carcinoma(82%), adenomatous goiter(33%) and hashimoto's thyroiditis(26%) but expressed a high appearance frequency in subacute thyroiditis(Table5). A follicular structure was observed in all nodular lesions except hurthle cell tumor and sub acute thyroiditis but expressed a high appearance frequency in follicular neoplasm(l00%) and grave's disease(l00%) (Table 5). Conclusively, a papillary structure and psammoma body of cytologic features change showed papillary carcinoma in FNAC but it is considered when diagnosing papillary carcinoma because nuclear grooves and intranuclear pseudoinclusion can be observed in other nodular lesions.