금강수계에서 하상재료에 따른 어류의 종다양성 및 서식지 평가

허준욱,인동수,장민호,강형식,강경호 한국환경영향평가학회 2011 환경영향평가 Vol.20 No.6

In order to establish fundamental data for stream restoration and environmental flow, we investigated inhabitation and species diversity of fish to substrate size in the typical streams of Geum River Basin. Field monitoring including fish sampling was conducted from October 2007 to October 2009. Substrate size was determined according to six different : silt (Si), sand (Sa), fine gravel (Fg), coarse gravel (Cg), cobbles (Co) and boulders (Bo). A total number of fish caught in the 18 sites was 7,649 representing 10 families 50 species, and Si, Sa, Fg, Cg, Co and Bo stations occupied 30, 29, 38, 30, 27 and 17 species, respectively. The most frequently found species in number was pale chum (Zacco platypus, 29.7%, n=2,275) followed by Z. koreanus (22.5%, n=1,720) in total stations. Biological diversity with increase of substrate size from the dominance of part species showed higher values as dominance index, lower and diversity, richness and evenness index. Index of biological integrity (IBI) and qualitative habitat evaluation index (QHEI) scores decreased with increase of substrate size. Therefore, it is necessary to make an effort on stream rehabilitation with evaluation of physical habitat condition by indicator species in order to maintain biodiversity and perform ecological restoration.

Stress response of olive flounder, Paralichthys olivaceus and Japanese croaker, Nibea japonica on changes of water temperature

허준욱 한국수산과학회 2022 Fisheries and Aquatic Sciences Vol.25 No.8

In this study, we investigate the physiological response of olive flounder, Paralichthys olivaceus and Japanese croaker, Nibea japonica, on a series of rising water temperatures (WT). At 1, 9, and 17 days, WT increased from 23℃ (control) to 26, 29 and 32℃ within 6 hours, respectively, and then maintained for 96 hours. At 5, 13, and 21 days, WT decreased from each WT to 23℃ within 6 hours and was maintained at 23℃ for 96 hours. Blood were collected at 0, 1, 5, 9, 13, 17, 21, and 60 days. In both species, hematocrit, red blood cell and hemoglobin of stressed groups were lower than non-stressed group at 13 days. The osmolality of stressed group was lower than non-stressed group at 21 and 60 days. In olive flounder, hemoglobin of stressed groups was lower than non-stressed group at 21 and 60 days, respectively, but osmolality of stressed group was higher than non-stressed group at 9 days. In Japanese croaker, hematocrit, red blood cell and hemoglobin of stressed groups were higher than non-stressed group at 21 and 60 days, respectively, and osmolality of stressed group was higher than that of non-stressed group at 1 day. At 1, 17, and 21 days, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) of stressed group were higher than non-stressed group in olive flounder. In Japanese croaker, AST and ALT of stressed group was lower than non-stressed group at 17, 21, and 60 days. The cortisol of olive flounder increased with increasing WT, but those of Japanese croaker increased with decreasing WT. In both species, the glucose increased with increasing WT. After changing WT, lactic acid of olive flounder and Japanese croaker were not significantly different. The physiological response of olive flounder increased directly to the increase in WT, and Japanese croaker contrasted with olive flounder.

Physiological Responses to Three Different Levels of Vibration Stress in Catfish, Silurus asotus

허준욱,김대희,이정열 응용생태공학회 2015 Ecology and resilient infrastructure Vol.2 No.4

The purpose of this study is to determine the effects of vibration on primary (e.g. plasma cortisol), secondary (e.g. plasma glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), Na+, K+ and Cl – ) and tertiary (e.g. mortality) stress responses in cultured catfish, Silurus asotus. For this purpose, three groups (one control group and two stress groups) were set up. The control group was exposed to vibration corresponding to 48 decibel (dB) volt (V) (produced using electric vibrators) for 15 minutes per hour every day, and the two stress groups were exposed to vibrations corresponding to 58 and 68 dB (V) (produced using the same electric vibrators), equally, for 15 minutes per hour every day. Blood was sampled at day 0 (before starting vibration stress tests), and at days 1, 3, 5, 7, 9 and 11 (after starting vibration stress tests). According to the results, the catfish physiologically showed ''typical'' stress responses when they were exposed to chronic vibration. This indicates that such chronic vibration caused substantial stress to catfish; especially, persistently elevated plasma AST and ALT levels observed caused adverse effects to them. In conclusion, chronic vibration could significantly affect hematological characteristics in catfish.

초강천에서 어류의 최적 생태유량 산정

허준욱,김대희,강형식,Hur, Jun Wook,Kim, Dae Hee,Kang, Hyeongsik 응용생태공학회 2014 Ecology and resilient infrastructure Vol.1 No.1

하천복원과 환경유량을 위한 기초자료를 확립하고자 금강수계의 초강천에서 생물학적 건강성 및 최적 생태유량에 대하여 조사하였다. 출현한 어종은 총 9과 36종 4,669개체였으며, 잉어과가 66.7%(24종)로 가장 높았다. 참갈겨니가 1,588개체로 34.0%로 우점종이었으며, 아우점종은 피라미로 22.6%, 우세종은 쉬리 13.3%로 나타났다. IBI와 QHEI는 상류로부터 하류로 내려갈수록 값이 감소하는 것으로 나타났으며, IBI 값은 27.9~38.6으로 평균 32.2로 양호상태를 나타냈다. 참갈겨니, 쉬리 및 감돌고기에 대하여 서식도적합도지수(HSI) 및 최적 생태유량을 산정하였다. 참갈겨니의 경우, 유속과 수심의 HSI 값은 각각 0.1~0.4 m/s와 0.2~0.4 m 였다. 감돌고기는 유속이 0.2~0.5 m/s, 수심이 0.4~0.6 m 및 하상재료가 가는자갈~호박돌로 나타났다. 이를 기초로 하여 각 지점의 최적 생태유량은 하류로 내려갈수록 값이 증가하는 것으로 나타났으며, 이에 따라서 WUA 값도 증가하는 것으로 나타났다. In order to establish fundamental data for stream restoration and environmental flow, we investigated optimal ecological flowrate (OEF) and riverine health condition in the Chogang Stream, a tributary to Geum River, Korea. The number of fish individuals sampled in this period were 4,669 in 36 species of 9 families. The most abundant species was Korean chub (Zacco koreanus, 34.0%) followed by pale chub (Z. platypus, 22.6%) and Korean shinner (Coreoleuciscus splendidus, 13.3%). Index of biological integrity (IBI) and qualitative habitat evaluation index (QHEI) values decreased from upstream to downstream along the stream. The estimated IBI value ranged from 27.9 to 38.6 with average 32.2 out of 50, rendering the site ecologically fair to good health conditions. OEF was estimated by the physical habitat simulation system (PHABSIM) using the habitat suitability indexes (HSI) of three fish species Z. koreanus, C. splendidus and Pseudopungtungia nigra selected as indicator species. In Z. koreanus, HSI for flow velocity and water depth were estimated at 0.1 to 0.4 m/s and 0.2 to 0.4 m, respectively. In P. nigra, HSI for flow velocity, water depth and substrate size were estimated at 0.2 to 0.5 m/s and 0.4 to 0.6 m and fine gravel to cobbles, respectively. OEF values increasing from up to downstream was found to increase, weighted usable area (WUA) values increased accordingly.

생태유량 산정을 위한 어류 모니터링 가이드라인 및 적용사례 - 1. 사전조사 -

허준욱,김정곤,Heo, Jun-Uk,Kim, Jeong-Gon 한국수자원학회 2011 물과 미래(한국수자원학회지) Vol.44 No.1

고수온에 따른 잉어(Cyprinus carpio)의 혈장 코티졸 농도 변화

허준욱,길현우,장민호,김선영,정현기,Hur, Jun Wook,Gil, Hyun Woo,Jang, Min Ho,Kim, Sun Young,Joung, Hyun Ki 한국해양생명과학회 2019 한국해양생명과학회지 Vol.4 No.2

잉어, Cyprinus carpio를 대상으로 고수온 스트레스 발생 시 코티졸 호르몬 변화 및 누적 사망률을 조사하였다. 수온 변화에 따른 20℃, 24℃ 및 28℃ 실험군의 코티졸 변화는 수온 변화 후 6시간에 코티졸 함량이 증가하다가 24시간 이후부터 감소하여 20℃ 및 24℃ 실험군은 실험 전의 코티졸 함량에 가까운 수치로 감소하였다. 48시간의 28℃ 실험군 코티졸 함량은 실험 전의 코티졸 함량보다 높았으나 24시간의 코티졸 함량보다는 유의한 차이를 보이며 감소하였다. 반면, 32℃ 실험군은 수온 변화 후 6시간부터 코티졸 함량이 증가하여 24시간에 코티졸 함량이 최대치로 증가하였으며 48시간까지 코티졸 함량이 감소하지 않고 유지되는 경향이 나타났다. 20℃, 24℃ 및 28℃ 실험군은 수온 변화 후 12시간까지 누적 사망률이 증가하다가 그 이후부터 사망하는 개체가 발생하지 않았으나, 32℃ 실험군은 실험 종료 시(48시간)까지 지속적으로 누적 사망률이 증가하는 경향이 나타났다. 본 연구로 도출된 결과를 이용하여 호르몬 분석을 통해 특정지역의 폐사 위험 정도를 확인할 수 있는 방안으로 활용 가능할 것이다. The changes of plasma cortisol concentration to high water temperature on common carp, Cyprinus carpio were studied. 20℃, 24℃ and 28℃ groups showed significantly increased plasma cortisol levels until 6 hours, and decreased plasma cortisol levels after 24 hours. Cortisol levels of 20℃ and 24℃ groups decreased to values close to the cortisol content before the experiment. However, 32℃ group showed significantly increased plasma cortisol levels until 24 hours, and maintained plasma cortisol level until 48 hours. The Cumulative mortality rates of 20℃, 24℃ and 28℃ groups increased until 12 hours, and maintained until 48 hours. Cumulative mortality rate of 32℃ group increased continuously until the end of the experiment. Using the results of this study, it could be used as a method to confirm the risk of mass perish in a specific area through hormone analysis.

마취 수송에 따른 양식 은어(Plecoglossus altivelis)의 혈액성상 변화

허준욱,박인석,장영진,고강희 한국해양과학기술원 2005 Ocean and Polar Research Vol.27 No.1

To assess the effect of anaesthetic on stress response in cultured sweetfish (Plecoglossus altivelis) during transportation, the levels of plasma cortisol, glucose, lactic acid, Na+, K+, Cl-, osmolality and survival were determined. The transportation was performed in square boxes where liquefied oxygen was saturated in polyethylene bags. Fish transportation was carried by car for 2 hours after anaesthesis with lidocaine-HCl/1,000 ppm NaHCO3 in experiment. Mean plasma cortisol concentration before transportation was 170.7 ng/ml. After transportation, the levels of plasma cortisol increased to 518.5 ng/ml (Control), 461.9 ng/ml (Sham control), 369.4 ng/ml (20 ppm anaesthetic), 304.0 ng/ml (40 ppm anaesthetic), 405.7 ng/ ml (80 ppm anaesthetic) and 499.1 ng/ml (160 ppm anaesthetic) in each experimental groups, respectively (P<0.05). However levels of glucose, lactic acid, Na+, Cl- and osmolality in 40 ppm anaesthetic group did not show significant differences in this before and after transportation (P>0.05). These result reveal an anaesthetic lidocaine HCl/1,000 ppm NaHCO3 is effective as sedative for transportation mixture in this species. This research provides baseline data on cortisol, glucose, lactic acid, Na+, K+, Cl-, osmolality and survival for anaesthetic transportation.

사육수의 저염분 변화에 따른 넙치(Paralichthys olivaceus)의 스트레스 반응

허준욱 부경대학교 수산과학연구소 2003 硏究業績集 Vol.27 No.-

용담댐 하류의 하천건강성 평가 및 어류 서식처를 고려한 최적 생태유량 산정

허준욱,김정곤 한국수자원학회 2009 한국수자원학회논문집 Vol.42 No.6

금강수계중 용담댐하류 앞섬 지점에서 생태유량을 산정하기 위한 생태 모니터링을 실시하여 어류종별 서식환경 특성조사를 포괄적으로 실시하였다. 이를 바탕으로 어류상, 상대풍부도 및 생물다양도 분석 등을 포함한 하천의 건강성 평가를 실시하고, 쉬리, 피라미 및 감돌고기 등에 대하여 작성한 서식처적합도 지수(HSI)와 물리적 서식처 모의시스템(PHABSIM)을 이용한 최적 생태유량을 산정하였다. 본 지점에서 채집된 종수는 20종이였으며, 우점종은 쉬리(22.4% In this study, a comprehensive field monitoring was conducted to understand habitat conditions of fish species in the upper Geum river. Based on the monitoring data, riverine health conditions such as composition ratio of fish species, richness and domina

태화강 내 연어(Oncorhynchus keta) 치어의 방류에 따른 성장 모니터링

허준욱,윤지우,임한규 (사)한국해양생명과학회 2021 한국해양생명과학회지 Vol.6 No.2

태화강에서 방류한 연어(Oncorhynchus keta) 치어의 성장, 생존 및 어류상 등의 모니터링를 통한 생태학적 기초 자료를 확보하고자, 방류 후 연어 치어의 지점별 포획개체수, 성장 및 어류상 특성을 조사하였다. 태화강 상류로부터 총 4개 지점[선바위교(St.1), 구영교(St.2), 삼호교(St.3), 명촌대교(St.4)]에서 2년간 조사를 실시하였다. 2017년 연어 치어 방류 후 전장은 평균 10 mm, 체중은 평균 0.8 g의 증가를 나타내었다. 2018년 연어 치어 방류 후 평균 전장 10 mm, 체중 0.5 g의 증가를 나타내었다. 연어 치어를 포식할 수 있는 어식성 어류는 St.1에서 6종(동사리, 숭어, 배스, 꺽지, 황어 및 끄리), St.2에서 5종(동사리, 황어, 배스, 꺽지 및 끄리), St.3에서 6종(큰가시고기, 가물치, 날망둑, 배스, 황어, 끄리) 및 St.4는 8종(큰가시고기, 베도라치, 문절망둑, 날망둑, 풀망둑, 강준치, 점농어 및 황어)이 출현하였다. 본 연구결과 연어 치어는 체중의 증가보다 전장 및 체장의 성장에 집중하는 것으로 나타났다. 또한, 적정 방류지역은 포식생물의 서식이 적고 수심, 유속, 염분 등의 변화가 적은 St.1과 St.2 지점이 적절할 것으로 판단되며 방류 시기는 끄리 및 배스 등의 포식어류의 먹이활동이 활발하지 않은 1월 초순 혹은 포식 활동이 적은 야간이나 새벽시간에 방류하는 것이 적절할 것으로 판단된다.