Antioxidant Effect of Citri Reticulatae Pericarpium Extract on Oxidative Stress-Mediated Cytotoxicity In Cultures

하대호,Ha, Dae-Ho 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.1

In order to examine oxidative stress of reactive oxygen species and the antioxidant effect of Citri Reticulatae Pericarpium (CRP) extract, human skin melanoma cells were treated with various concentrations of hydrogen peroxide ($H_2O_2$). Antioxidant effect of CRP extract on $H_2O_2$-induced cytotoxicity, cell viability, DPPH-radical scavenging activity and superoxide dismutase (SOD)-like activity. In this study, $H_2O_2$ decreased cell viability of cultured human skin melanoma cells in dose- and time-dependent manners, and then, midcytotoxicity value (MCV) was determined at $60\;{\mu}M$ after human skin melanoma cells were cultured for 5 hours in the media containing $20{\sim}60\;{\mu}M$ of $H_2O_2$, respectively. The $H_2O_2$ was on cultured human skin melanoma cells because MCV of $H_2O_2$ was lower than $100\;{\mu}M$. In the antioxidant effect of CRP extract, CRP extract increased cell viability DPPH-radical scavenging activity and SOD-like activity. From these results, it is suggested that $H_2O_2$ was very toxic on cultured human skin melanoma cells. And also, CRP extract has the antioxidant effect on $H_2O_2$-induced cytotoxicity.

족저 내측부의 동정맥 기형: 증례 보고

하대호,권정남,김유미,이준형,Ha, Dae-Ho,Kwon, Jung-Nam,Kim, Yu-Mi,Lee, Jun-Hyung 대한족부족관절학회 2016 대한족부족관절학회지 Vol.20 No.4

Arteriovenous malformation (A-V malformation) is defined as an abnormal connection between arteries and veins that lead to A-V shunting with an intervening network of vessels. A-V malformation is a rare condition, and spontaneous regression is also rare. A-V malformation becomes symptomatic when the surrounding tissue and osseous structures are negatively affected. A-V malformation has a high recurrence rate and is relatively hard to treat. In this case, a huge mass with pulsatile and bruit on the medial plantar area were observed. With the diagnosis of A-V malformation in accordance with the results from ultrasonography, magnetic resonance imaging and computed tomography angiography, and mass excision with feeding vessel ligation through plantar midfoot approach was completed successfully.

하퇴부와 족관절 및 족부의 피부 결손에 대한 역행성 표재 비복동맥 피판을 이용한 재건의 장점

하대호,김상수,전철홍,김동철,최유선,김경진,Ha, Dae-Ho,Kim, Sang-Soo,Chun, Churl-Hong,Kim, Dong-Churl,Choi, Yu-Sun,Kim, Kyeong-Jin 대한미세수술학회 2001 Archives of reconstructive microsurgery Vol.10 No.2

Introduction : We report advantages of distally based superficial sural artery flap in the soft tissue defect in lower leg, ankle and foot. They are easier and less complicated technique than others. Material & Method : Total 26 patients were operated and their soft tissue defect site were 10 cases of anterior tibial surface, 6 cases of Achilles tendon area, 3 cases of lateral and medial surface of tibia, 3 cases of foot dorsum surface, 3 cases of sole, 1 case of medial aspect of ankle. Average pedicle distance was 12.8 cm(range $8{\sim}21cm$) and follow up period was mean 18 months. Result : We obtained 24 cases of excellent and good results. The two cases were reported low complication rate, which were one case of skin necrosis covered full thickness skin graft, and the other case of infection. Conclusion : The advantage of distally based superficial sural artery flap in soft tissue defect are long distant pedicle, short operation time, easy elevation of pedicle, constant and reliable blood supply and good cosmetic result with thin-thickness flap.

초음파와 방사선 투시장치를 이용한 경추 신경근 차단술의 임상결과 비교

하대호(Dae Ho Ha),심대무(Dae Moo Shim),김태균(Tae Kyun Kim),오성균(Sung Kyun Oh),이현준(Hyun Jun Lee) 대한정형외과학회 2020 대한정형외과학회지 Vol.55 No.1

목적: 초음파 유도하 경추 신경근 차단술을 시행한 환자들의 임상결과를 방사선 유도하 경추 신경근 차단술을 시행한 환자들과 비교하고 합병증 및 수술 비율 등에 대해 비교하고자 하였다. 대상 및 방법: 경추 추간판 탈출증으로 진단된 환자들에 대해 2015년 5월 1일부터 2018년 2월 8일까지 초음파 유도하 경추 신경근 차단술을 시행한 97예와 2010년 6월 1일부터 2017년 8월17일까지 방사선 유도하에 시행한 94예 환자들의 경추통과 방사통을 시술 전과, 3주, 6주 후 verbal numeric rating scale (VNRS)을 이용하여 조사하였고 시술과 연관된 합병증 유무도 의무기록을 통해 조사하였다. 결과: 초음파 유도하 경추 신경근 차단술을 시행한 97예 중 66예가 연구에 포함되었고 이들의 평균 연령은 57세였다. 남성이 41명 여성이 25명이었고 목표 신경근은 경추 5번이 8예, 6번이 37예, 7번이 21예였다. 방사선 유도하 경추 신경근 차단술을 시행한 94예 중 77예가 연구에 포함되었고 이들의 평균 연령은 55세였다. 남성이 40명, 여성이 37명이었고 목표 신경근은 5번이 6예, 6번이 42예, 7번이 26예, 8번이 3예였다. 시술 후 경추통의 numeric rating pain scale은 초음파 유도하의 경우 중간값 5.4점에서 3주, 6주에 2.7점, 1.4점으로 감소하였고(p=0.0023, p<0.001), 방사선 유도하의 경우 시술 전 중간값 6.2점에서 3주에 3.1점, 6주에 1.5점이었다(p<0.001, p<0.001). 방사통의 경우 초음파 유도하 차단술의 경우 시술 전 중간값 6.3점에서 3주에 2.8점, 6주에 1.5점으로 호전되었고(p<0.001,p<0.001), 방사선 유도하의 경우 시술 전 7.4점, 3주, 6주에 3.3점, 1.9점으로 감소하였다(p<0.001, p<0.001). 합병증의 경우 방사선 유도하 차단술의 경우 호너 증후군이 1예, 간대성 근경련이 1예였으며 초음파 유도하 차단술의 경우 일시적인 수부 부종을 호소하는 1예가 있었다. 결론: 초음파 유도하 경추 신경근 차단술을 시행한 환자들의 임상결과는 방사선 유도하 경추 신경근 차단술을 시행한 환자들과 통계적 차이를 보이지 않았다. Purpose: This paper compares the clinical outcomes of patients who were treated with a cervical nerve block by ultrasound and C-arm and reports the complication. Materials and Methods: A total of 97 patients were treated with an ultrasound-guided nerve root block from May 1, 2015 to February 8, 2018. On the other hand, 94 patients were treated with a C-arm guided nerve root block. The consequences of the cervical pain and the radiating pain before and after the procedures were reviewed using the verbal numeric rating scale (VNRS). In addition, the complications related to the procedures from the daily notes from the chart were inspected. Results: Sixty-six cases out of 97 cases of ultrasound-guided nerve root block were enrolled in the study. The average age of the patients was 57 years, including 41 males and 25 females. Seventy seven out of 94 cases by a C-arm guided root block were included in the study. The average age of the patients was 55 years, including 40 males and 37 females. Before the nerve root block, the mean numeric rating pain scale (NRS) of the cervical pain in ultrasound-guided block decreased from 5.4 points to 2.7 points at three weeks and 1.4 points at six weeks (p=0.0023, p<0.001), and 3.1 points in the C-arm (p<0.001, p<0.001) at three weeks and 1.5 points at six weeks (p<0.001, p<0.001). In the case of radiating pain, the mean NRS in the ultrasound-guided nerve root block group improved from 6.3 points after the procedure to 2.8 points at three weeks and 1.5 points at six weeks (p<0.001, p<0.001). In the C-arm guided nerve root block group, the NRS improved from 7.4 points after the procedure to 3.3 points at three weeks and 1.9 points at six weeks. In the case of complications, Horner"s syndrome and propriospinal myoclonus were observed in one case of C-arm guided block group. Conclusion: The clinical results of the patients who underwent ultrasound-guided cervical nerve root block were not significantly different from those who underwent a C-arm guided cervical nerve root block.

근육통증 유발 동물모델에서의 조직학적 소견

하대호 ( Dae Ho Ha ),조용우 ( Yong Woo Cho ),최유선 ( Yoo Sun Choi ),이강창 ( Kang Chang Lee ),이종화 ( Jong Hwa Lee ) 대한통증학회 2004 The Korean Journal of Pain Vol.17 No.1

시엽추출물이 활성산소로 손상된 멜라닌세포종의 멜라닌합성 및 세포생존율에 미치는 영향

하대호(Dae Ho Ha),이재규(Jae Kyoo Lee),최유선(Yu Sun Choi) 한의병리학회 2008 동의생리병리학회지 Vol.22 No.5

수근 주상골 단독 탈구

하대호(Dae Ho Ha),김상수(Sang Soo Kim),송정인(Jeong In Song) 대한정형외과학회 1999 대한정형외과학회지 Vol.34 No.5

뇌전증 발작 후 발생한 다발성 흉추 골절

하대호(Dae-Ho Ha),오성균(Sung-Kyun Oh),김승민(Seung-Min Kim) 대한정형외과학회 2020 대한정형외과학회지 Vol.55 No.3

뇌전증에 의한 전신 발작 시 발생한 강력한 근육 수축에 의한 근골격계 손상이 발생할 수 있다. 외상 및 기저질환 없이 전신 발작 후 발생한 다발성 흉추 골절에 대한 국외 증례는 많지만 국내의 연구나 증례보고는 없다. 이전에 골다공증이나 특이 질환이 없는 42세 남자 환자의 수면 중 전신 간대성 발작에 의한 다발성 흉추 골절을 진단하였기에 증례를 보고하는 바이다. The musculoskeletal system can be damaged by massive contractions of the muscles in the case of systemic attacks by epilepsy. Several studies or case reports of multiple thoracic vertebrae fractures following generalized seizures without underlying diseases or falls have been reported, but there are few domestic studies or case reports. This paper reports the case of a 42-year-old male patient without any specific underlying disease, who was diagnosed with multiple fractures of the thoracic vertebrae caused by generalized myoclonic epilepsy during sleep.

메틸수은으로 손상된 배양 심근세포에 대한 冬瓜子의 영향

하대호(Dae Ho Ha),양현웅(Hyun Woong Yang),이강창(Kang Chang Lee) 한의병리학회 2003 동의생리병리학회지 Vol.17 No.5

요추 극외측 추간판 탈출증과 후외측 추간판 탈출증의 수술에 대한 상대 위험도

하대호(Dae Ho Ha),심대무(Dae Moo Shim),김태균(Tae Kyun Kim),오성균(Sung Kyun Oh),김진(Jin Kim) 대한정형외과학회 2017 대한정형외과학회지 Vol.52 No.5

목적: 코호트 연구를 통해 요추 후외측 탈출증과 극외측 탈출증 환자의 수술에 대한 상대 위험도를 알아보고자 하였다. 대상 및 방법: 요추 추간판 탈출증으로 진단된 환자 중 일측 방사통에 대해 경추간공하 경막외 차단술을 시행 받은 총 295명의 환자에 대해 후향적 코호트 연구를 시행하였다. 극외측 탈출증으로 진단된 34명과 후방 혹은 후외측 탈출증으로 진단된 환자는 261명이었다. 이들의 중앙 추시 기간은 23.9개월이었고, 평균 연령은 55.7세, 남성은 131명, 여성은 164명이었다. 결과: 추시 중 총 51명이 수술을 시행 받았으며, 38명은 후방 혹은 후외측 추간판 탈출증이었고 13명은 극외측 추간판 탈출증이었다. 척추관내 추간판 탈출증에 비해 극외측 탈출증이었던 경우 수술에 대한 상대 위험도(relative risk)와 95% 신뢰구간(95% confidence interval)은 2.67 (1.59-4.48)이었으며 이러한 관련성은 통계적으로도 유의하였다(p=0.0002). 극외측 추간판 탈출증 환자들의 수술군과 비수술군의 최종 추시 방사통에 대한 평균 시각통증등급(visual analogue scale)은 0.69, 1.50으로 비수술군이 유의하게 높았고(p=0.035), Korean-Oswestry Disability Questionnaire는 각각 11.7점, 9.6점 유의한 차이를 보이지는 않았다(p=0.266). 결론: 극외 탈출증의 경우 척추관내 탈출증에 비해 신경차단술 후 수술에 대한 상대적 위험도가 2.67로 높아 보다 적극적으로 수술을 고려해야 한다. Purpose: We reported relative risk of operation between patients who were diagnosed with lumbar far lateral disc herniation and posterolateral disc herniation after transforaminal epidural block. Materials and Methods: A retrospective cohort study was performed on 295 patients (131 male and 164 female) who underwent transforaminal epidural block for unilateral radiculopathy caused by lumbar intervertebral disc herniation. Among these 295 patients, 34 patients were diagnosed with far lateral disc herniation and 261 patients with posterolateral disc herniation. The median follow-up period was 23.9 months, with an average age of 55.7 years. Results: Fifty-one patients underwent surgical treatment. Among them, 38 patients had posterolateral disc herniation and 13 patients had far lateral disc herniation. Patients with far lateral disc herniation, when compared with posterolateral disc herniation, had a relative risk of operation of 2.67 (1.59-4.48, 95% confidence interval) with statistical significance (p=0.0002). At the final follow-up, the average visual analogue scale (VAS) score for radicular pain in the surgical and non-surgical groups was 0.69 and 1.50, respectively; the average Korean-Oswestry Disability Questionnaire (K-ODI) score was 11.7 and 9.6, respectively. The difference of average VAS score showed a statistical significance (p=0.035), however, that of K-ODI showed no statistical significance (p=0.266). Conclusion: In the case of far lateral disc herniation, the relative risk of surgery after transforaminal epidural block was 2.67, which is higher than that of posterolateral disc herniation. Therefore, we should consider surgical treatment more aggressively.