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최옥희,김진우,류충민,박창석 한국식물병리학회 2004 Plant Pathology Journal Vol.20 No.2
Paenibacillus polymyxa E681, with its plant growth promotion and root colonization ability, has been proven to be a promising biocontrol agent of cucumber and barley. This study investigated the attributes related to the movement of bacteria from the seed to the radicle and to the whole root system. It also illustrated the existing form and population changes of the bacteria on seed and root using the scanning electron microscope and confocal laser scanning microscopy. The bacteria invaded and colonized the inside of the seed coat while the seeds were soaked in bacterial suspension. Almost the same number of bacteria on seed surface invaded the inside of the seed coat right after seed soaking. The population densities of E681 increased greatly inside as well as on the surface of the seed before the radicle emerged. The bacteria attached on the emerging radicle directly affected the initial population of newly emerging root. The colonized cells on the root were arranged linearly toward the elongation of the root axis. In addition to colonizing the root surface, strain E681 was found inside the roots, where cells colonized the intercellular space between certain epidermal and cortical cells. When the cucumber seeds were soaked in bacterial suspension and sown in pot, the bacterial populations attached on both the surface and inside of the root were sustained up to harvesting time. This means that E681 successfully colonized the root of cucumber and sustained its population density up to harvesting time through seed treatment.
적응유연성 증진 집단상담 프로그램이 학습부진아의 학습된 무기력과 학교생활적응에 미치는 영향
최옥희,최병연 한국초등상담교육학회 2014 초등상담연구 Vol.13 No.1
본 연구의 목적은 적응유연성 증진 집단상담 프로그램이 학습부진아의 학습된 무기력과 학교생활적응에 미치는 영향을 검증하는 것이다. 연구의 대상은 J시에 소재하는 D초등학교 5~6학년 학습부진아 24명(실험집단: 12명, 통제집단: 12명)이었다. 실험집단에 총 12회기 동안 적응유연성 증진 집단상담 프로그램을 실시한 다음 그 효과를 검증하였다. 그 결과 적응유연성 증진 집단상담 프로그램에 참여한 실험집단과 통제집단 간에 학습된 무기력 전체 점수와 모든 하위 요인 점수에 유의한 차이가 있는 것으로 나타났다. 또한 학교생활적응 전체 점수와 하위 요인 점수를 분석한 결과 집단 간에 통계적으로 유의한 차이가 나타났다. 따라서 본 연구에서 사용한 적응유연성 증진 집단상담 프로그램은 학습부진아의 학습된 무기력 감소와 학교생활적응력 신장에 효과가 있는 것으로 밝혀졌다.
미술의 잠재적 공간 : 창의력, 감응과 관습에 대한 도전
최옥희,Nicholas Addison 한국국제미술교육학회 2012 미술과 교육 Vol.13 No.1
나는 창의적인 활동을 지원하고 유지하는 환경적인 조건의 고찰을 통해, 창의성이 학교 미술교육을 발전시킬 수 있게 만드는 조건들을 조사하였다. 이 과정에서 주로 생산 과정으로서 창의력이 갖는 순화된 특성을 비평할 뿐만 아니라 비고츠키식 학습 개념 안에서 사회적 이벤트로서 그림을 그리는, 창의적인 사람들을 비평하였다. 후자는 학생들이 그리기 "게임"을 통해 얻은 허가를 이용하여 교사를 "공격"하는 관습을 거스르는 사례를 통해 살펴보았다. 이는 클라인(Klein)의 이론과 그녀의 유아적 공상의 지속성에 대한 주장, 그리고 창의적인 행동에 있어 그것이 갖는 중요성과 연관되어 이론화 되었다. 나는 이러한 생각들을 합치기 위해 위니코트(Winnicott)의 "잠재적 공간" 개녕믕 안전한 모체 환경을 통해 아이들이 창의성에 필요한 위험을 감수할 수 있게 해주는 공간으로 이끌어내었다.
최옥희,김석현,김진우 경상대학교 농업생명과학연구원 2014 농업생명과학연구 Vol.48 No.5
Sesame is a major cooking oil crop in Korea. One of the primary problems in sesamecultivation is low healthy stand establishment due to the occurrence of seedling rot anddamping-off resulting from a complex of soil-borne pathogens in the field. To address theproblem, the bioformulation of Pseudomonas fluorescens M45 was prepared in powder formusing clay and vermiculite, and was evaluated for its effect on biological control of soil-bornepathogens in sesame cultivation. In the petri dish trial, the emergence rate was overall good (>92%) regardless of seeds being pelleted and/or M45-treated. In both pot and field trialscontaining disease-conducive soils, seed-pelleting substantially reduced emergence rate, whereasseed-pelleting with M45 significantly improved the emergence rate (> 26%). The emergence rateof sesame seeds treated with the strain M45 was greater than 30% regardless of seedpelletization. We also found that M45r colonized in the roots at the density of 1.6×105 cfu/g. With aid of the bioformulation, however, root colonization of the strain was significantlyincreased to 4.0×106 cfu/g. The powder formulation with strain M45 enhanced the rate ofhealthy stand establishment in disease-conducive soil. Therefore, bioformulation with strain M45is a promising method to overcome problems associated with the successive cultivation ofsesame.
최옥희,곽연식,김진우 경상대학교 농업생명과학연구원 2013 농업생명과학연구 Vol.47 No.3
Pseudomonas fluorescens B16 has been shown to be a promising biological control agent for the promotion of plant growth, root colonization, and suppression of bacterial wilt. This study investigated the dispersion of B16 from seed to radicle, and to the whole root system, and illustrated population changes within cucumber seeds and radicles using scanning electron microscopy (SEM), fluorescence microscopy (FM) and immunofluorescence microscopy (IFM). When cucumber seeds were soaked in a bacterial suspension of B16, bacterial cells entered the seeds through the pore at the base, colonized the inner plane, and proliferated germination advanced. Population densities of B16 were gradually increased, and increased initial populations within newly growing roots. The root surface was covered by cylindrical rod-shaped bacterial cells, and junctions between epidermal cells were covered preferentially. Previous studies exploring root colonization of rhizobacteria have not focused on colonized populations inside seeds. Our microscopic observations confirmed the importance of bacterial cells colonized inside seed coats. Population densities of B16 were then naturally sustained up to harvesting time. The results obtained in this study provide about a novel insight into the commercial application of biological control agents within seeds.
최옥희,류충민,김진우 경상대학교 농업생명과학연구원 2013 농업생명과학연구 Vol.47 No.1
We analyzed the viable population changes of Paenibacillus polymyxa E681, a plant growth-promoting rhizobacterium, on seeds and roots after bioformulation at varying time intervals during the storage. The viable population of E681 on tested crop seeds sustained log 4-5 cfu/seed after 300 days of seed treatment. The ability of root colonization and inhibition of fungal mycellial growth was not influenced even after 300 days of seed treatment. The seed-soaking treatment returned better results than powder formulation, in increasing the initial population of E681 on plant roots. Collectively, it was found that E681 is a durable and stable biological control agent for application to crop seeds.