Drosophila melanogaster 에 미치는 DDVP 의 생리변이적 영향에 관한 연구

최영현,박연규,이원호 ( Yung Hyun Choi,Yeon Kyu Park,Won Ho Lee ) 한국환경생물학회 1994 환경생물 : 환경생물학회지 Vol.12 No.2

DDVP(dichlorvos, 2, 2-dichlorovinyl dimethyl phosphate), the organophoso phorus pesticide, was fed to the Drosophila melanogaster complex in order to investigate its toxic capacity at development of D. melanogaster larvae and four species adults of the D. melanogaster complex(D. mauritiana, D. simulans, D. mauritiana, and D. sechellia). And the potency of DDVP for the induction of the X-linked lethal and somatic chromosomal mutations of D. melanogaster was studied. For these purposes, an attached-X method for germinal cell level and a wing hairs spot test system(mwh/flr system) for somatic cell level were appllied. DDVP was highly toxic on the development of D. melanogaster, resulting in of lowering the viability and in prolongation of the developmental time. The order of mortality causing adult stage feeding to DDVP in the D. melanogaster complex was like this; D. simulans, D. mauritiana, D. sechellia and D. melanogaster. The effect on the sex-linked lethal mutagenesis using an attached-X method was found to be negative. But the frequency of small single mwh spots due to terminal deletion or gene mutation on chromosome 3 in the transheterozygouse(mwh +/+ flr) larvae treated with DDVP were slightly higher than the control group.

Dexamethasone에 의하여 유발된 근육 위축 생쥐의 비복근 근섬유에서 apoptosis와 염증 반응에 미치는 오미자 추출물의 영향

최영현,Choi, Yung Hyun 대한한의학방제학회 2017 大韓韓醫學方劑學會誌 Vol.25 No.3

Dried fruits of Schizandra chinensis Baillon, Fructus Schisandrae, have been widely used for many years to prevent and treat various diseases in Asian countries including Korea and Russia. It has recently been reported that extracts of Fructus Schisandrae are effective for controlling muscle and skeletal diseases. In this study, we investigated the efficacy of ethanol extract of Fructus Schisandrae (EEFS) on apoptosis and inflammatory response in gastrocnemius muscle of dexamethasone-induced catabolic muscle atrophy mice as part of natural substance discovery and functional analysis for improving muscle function. According to the results of this study, EEFS supplementation attenuated body weight gains and suppressed calf thickness loss in dexamethasone-induced muscle atrophic mice. Gastrocnemius muscle immunohistochemistry showed that expression of caspase-3 and poly(ADP-ribose) polymerase, which are representative apoptotic markers, was markedly increased in dexamethasone control mice; however, their expression was effectively reduced in the EEFS-fed mice. EEFS supplementation also prevented dexamethasone-induced increases in immunoreactivity of muscle fibers for myostatin, an important negative regulator of skeletal muscle mass. In addition, EEFS significantly normalized the increased numbers of nitrotyrosine, 4-hydroxynonenal and inducible nitric oxide synthase-positive muscle fibers compared to that found in dexamethasone control mice. These results suggest that EEFS protects dexamethasone-induced muscular atrophy by decreasing apoptosis and inflammatory responses, and EEFS is more likely to be developed as a muscle strengthening agent.

Drosophila 수종의 성즐변이에 관한 형태적 비교 연구

최영현,권용원,유미애,이원호,Choi, Yung-Hyun,Kwon, Yong-Won,Yoo, Mi-Ae,Lee, Won-Ho 한국응용곤충학회 1994 한국응용곤충학회지 Vol.33 No.4

Drosophila 의 수컷 앞다리 부절에 존재하는 성즐의 유무는 Sophophora subgens와 Drsophila sub-genus의 분류기준이 되며, 전자의 경우는 종 특이적인 성즐을 가지나 후자에는 일반적으로 존재하지 않는다. 수종 Drosophila 의 성즐에 관하여 비교 조사한 결과 Sophophora subgens내의 D. melanoga-ster subgroup의 경우는 모두 제 1부절에만 존재하였다. 그중 D. melanogaster complex의 D. mauti-tiana 가 약 12.75개, D. simulans는 평균 8.35정도였으며, D. yakuba complex는 D. melanogaster complex에 비하여 다소 적었다. D. melanogaster complex의 종간 교배에 의한 분석으로는 성즐유전양시겡 대한 뚜렷한 방향성을 제시할 수 없었다. Sophophora subgemus의 D. melanogaster species group에 속하는 다른 3종의 경우(D. auralia, D. lutecens 및 D. suzuki)는 성즐의 분포 양식에서 D. immigrans 및 D. virilis)에서는 성즐이 존재하지 않았다. Sex combs which lack in females are characteristic of some members of the subgenus Sophophora When present, they can be van/ from a single blistle to very extensive structures, but the meianogaster subgroup of the subgenus Sophophora have only on the f~rst tarsal segments of the fore legs. D mauritiona among the melanogaster compler of the melar~ogaster subgroup have significantly more sex comb teeth (mean 12.75) than other specie& and the yakubo complex (range of the mean 6.84-758) have less than the melanogaster complex (range of the mean 835-12.75). In sex comb tooth number of interspecd~ch ybrids among the melonogoster complex, there appeared difference u;ith parental species Thus, analysis of male sex comb tooth in FI hybrid could not exhibit the direction of hereditan/ nature. On the other hand, D auraria (the montium subgroup), D suzuki (the suzuki subgroup) and D, lutecens (the takohashii subgroup) of the melanogaster species have two sex combs on the first and second tarsal segments, and the mean number were different according to the species.

상황버섯 균사체 발효주의 세포독성 비교

최영현,박철,정일홍,최병태,이용태,박동일,정영기,Choi Yung Hyun,Park Cheol,Jung Il Hong,Choi Byung Tae,Lee Yong Tae,Park Dong Il,Jeong Young-Kee 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

Phellinus linteus (Berk. & M.A. Curtis) Teng, commonly referred to as Sangwhang in Korea, is a well-known species of the genus Phellinus, which attracts great attention due to its phamarcological values. P. linteus has been reported to produce anti-tumor, anti-angiogenic, anti-mutagenic and immunomodulatory activities in vivo and in vitro. However, despite extensive biochemical studies on P. linteus, the wine produced by P. linteus fermentation (WPLF) has poorly investigated. In the present study, it was compared the in vitro cytotoxic effects of WPLF with ethanol as positive control. WPLF as well as ethanol induced the inhibition of cell proliferation and morphological changes in both HepG2 and A549 cells in a concentration-dependent manner, however, WPLG treatment has less cytotoxic effects than ethanol treatment. These cytotoxic effects were associated with the induction of apoptotic cell death, but, WPLG treatment has less apoptotisis inducing effects than ethanol treatment.

Drosophila melanogaster 에 미치는 제초제의 생리변이적 영향에 관한 연구

최영현,박연규,유미애,이원호 ( Yung Hyun Choi,Yeon Kyu Park,Mi Ae Yoo,Won Ho Lee ) 한국환경생물학회 1996 환경생물 : 환경생물학회지 Vol.14 No.2

Glyphosate (isopropylamine salt of N-(phosphono methyl)) and glufosinate ammonium (ammonium 4-[hydroxy(methyl) phosphinoyl]), the herbicides, were fed to Drosophila melanogaster in order to compare their physiological toxic and mutagenic effects. These herbicides were highly toxic on the development of D. melanogaster, resulting in lowering the viabilities from larvae to adults and prolongation of the developmental times, and glufosinate ammonium was slightly more toxic than glyphosate. As to the sex ratio of adult flies fed these herbicides during the development, there was no differences between the control and the treated groups. We also tested the somatic cell mutageneic potencies of these herbicies using a Drosophila wing hairs spot test system (mwh/flr system). The frequencies of small single mwh spots due to terminal deletion or gene mutation on chromosome 3 in the transheterozygouse (mwh +/ + flr) larvae treated with these herbicies were 3∼4 times higher than those of the control group. These results seem to suggest that glyphosate and glufosinate ammonium may exert their mutagenic properties.

Mouse의 정소이용 염색체 조사에 G- 및 C-banding법의 적용

최영현,권용원,윤희선,유미애,이원호,Choi, Yung-Hyun,Kwon, Yong-Won,Yun, Hee-Sun,Yoo, Mi-Ae,Lee, Won-Ho 한국생명과학회 1998 생명과학회지 Vol.8 No.1

The mototic and meiotic chromosomal characteristics of ICR mice were investigated with G-and C-banding techniques. For the puposes, the chromosomal preparations were made with the modified air-drying method of Imal et al. Chromosomal analysis using testis could be observed mitotic as well as meitotic chromosomal behaviors, and the centromeric regions of all chromosomes including X chromosome were strongly stained in C-banded preparations. Nineteen autosomal bivalents and a single uniequal terminally associated X-Y bivalent in normal cells were observed during the late prophase and the metaphase of the meiosis I. The mean frequencies of previously dissociated X-Y chromosomes in the primary apermatocytes of the control group were 7.45%, but the frequencies of X-Y dissociation in the alkylating agents-treated group were about 3-4 times higher than that in the control group. Application of C-banding in meiotic stages could be certainly distinguish between vibalent type and univalents type of sex chromosomes. G- 및 C-banding법에 의한 ICR 생쥐의 유사분열과 감수분열 시기의 염색체 특징에 관하여 조사하였다. 이를 위한 염색체 표본 작성은 Imai et al.의 공기건조법을 다소 변형한 방법에 의하였다. 정소를 이용한 염색체 분석은 유사분열뿐만 아니라 감수분열단계들을 모두 관찰할 수 있었으며, C-banding에 의하여 X 염색체를 포함한 모든 염색체의 돈원체 부위가 강하게 염색되었다. 정상의 제 1 감수분열 전기와 중기 사이의 세포들에서 2가 염색체로 된 19쌍의 상염색체과 1개의 말단결합으로 된X-Y 염색체를 관찰할 수 있었다. 대조군의 제 1 정모세포에서 조기분리된 성염색체를 가지는 세포의 빈도는 약 7.45%였으나,alkylating agents 처리군에서는 대조군에서보다 약 3-4배 이상 높에 나타났다. 그리고 감수분열 단계의 염색체 표본에 C-banding을 적용함으로서 X-Y 염색체 조기분리의 관찰이 매우 용이하였다.

Drosophila melanogaster 에 미치는 Kojic acid 의 독성에 관한 연구

최영현,박연규,이원호 ( Yung Hyun Choi,Yeon Kyu Park,Won Ho Lee ) 한국환경생물학회 1996 환경생물 : 환경생물학회지 Vol.14 No.1

The present experiment was carried out to detect the basic toxic effect of kojic acid (5-hydroxy-2-hydroxymethyl-γ-pyrone), a fungal metabolite produced by some species of Aspergillus and Penicillium, in Drosophila melanogaster. Kojic add was highly toxic on the developmental and adult stages, resulting in prolongation of the developmental time and lowering of the viability from larvae to adult, and high mortality of adults as dose increased. The LC_50 value at 72 hr i.e., the concentration at which 50% of treated flies died within 72hr, was about 4.8㎎/㎖. As to the sex ratio of flies fed kojic acid during the development, there was no differences between the control and the treated groups. And, kojic acid gave no a significant mutagenic effect on the induction of the sex-linked lethal mutagenesis by the attached-X method used in this study.

비소화합물에 의한 A549 폐암세포의 증식억제에 관한 연구

최영현,최우영,최병태,이용태,이원호,Choi, Yung-Hyun,Choi, Woo-Young,Choi, Byung-Tae,Lee, Yong-Tae,Lee, Won-Ho 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

Recently, arsenic compounds were considered as novel agents for treatment of acute promyelocytic leukemia and malignant tumors. However, it showed severe toxicity effect on normal tissue at the same time. In this study, to investigate the possible molecular mechanism (s) of arsenic compounds as candidate of anti-cancer drugs, we compared the abilities of two arsenic compounds, tetraarsenic oxide $(AS_4O_6)$ and arsenic trioxide (diarsenic oxide, $As_2O_3$), to induce cell growth inhibition as well as apoptosis induction in A549 human non-small cell lung cancer cells. Both $As_4O_6\;and\;As_2O_3$ treatment declined the cell growth and viability of A549 cells in a concentration-dependent manner, which was associated with induction of G1 arrest of the cell cycle and apoptotic cell death. However, $As_4O_6$ induced growth inhibition and apoptosis in A549 cells at much lower concentrations than $As_2O_3.\;As_4O_6$ down-regulated the levels of anti-apoptotic Bcl-2 protein, however, the levels of Bax, a pro-apoptotic protein, were up-regulated in a dose-dependent manner. In conclusion, $As_4O_6$ might be a new arsenic compound which may induce apoptosis in A549 cells by modulation the Bcl-2 family and deserves further evaluation.

Genistein Suppresses TPA-Induced Matrix Metalloproteinases Activity and Cell Invasion in Human Breast Adenocarcinoma Cells

Yung Hyun Choi(최영현),Sung Ok Kim(김성옥) 한국생명과학회 2012 생명과학회지 Vol.22 No.7

Genistein은 대두 및 그들의 부산물에 풍부하게 존재하는 isoflavone의 일종으로 정상세포에서는 독성을 나타내지 않는 범위에서 다양한 in vitro 및 in vivo 모델에서 암세포의 증식을 효과적으로 억제할 수 있는 천연물로 알려져 있다. 본 연구에서는 MCF-7 및MDA-MB-231 유방암세포에서 matrix metalloproteinases (MMPs)의 활성 및 발현과 침윤성에 미치는 genistein의 영향을 조사하였다. 본 연구의 결과에 의하면 genistein은 12-O-tetradecanoyl phorbol-13-acetate (TPA) 처리에 의하여 활성화된 MMP-2 및 -9의 활성을 유의적으로 차단하였으며, 이는 전사 및 번역 수준에서 MMP-2 및 -9의 발현 억제와 연관성이 있었다. 또한 matrigel invasion assay를 통하여 genistein은 두 유방암세포의 침윤성을 완벽하게 차단하였음을 관찰하였으며, 이러한 효과는 genistein의 세포독성 효과에 의한 것이 아니었음을 알 수 있었다. 비록 in vivo 동물 실험을 통한 부가적인 연구의 필요성이 있으나, 본 연구의 결과는 genistein이 암의 전이를 억제할 수 있는 효과적인 식이 소재임을 보여주는 것이다. Genistein, a predominant isoflavone, has been shown to inhibit the growth of various cancer cells in vitro and in vivo without toxicity to normal cells. In the present study, we investigated the effects of genistein on the activity and the expression of matrix metalloproteinases (MMPs) in MCF-7 and MDA-MB-231 human breast adenocarcinoma cells. Our findings showed that MMP-9 and -2 activation was significantly increased in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). However, the increased activities of MMP-9 and -2 in TPA-treated cells were concentration-dependently inhibited by treatment with genistein, and this was also correlated with a decrease in the expression of their mRNA and proteins. In addition, a matrigel invasion assay showed that genistein reduced TPA-induced invasion of MCF-7 and MDA-MB-231 cells. Although further in vivo studies are needed, these results suggest that genistein treatment may inhibit tumor cell invasion and, therefore, act as a dietary source to decrease the risk of cancer metastasis.

Inhibition of Cell Invasion by Indole-3-Carbinol in OVCAR-3 Human Ovarian Cancer Cells

Yung Hyun Choi(최영현),Sung Ok Kim(김성옥) 한국생명과학회 2011 생명과학회지 Vol.21 No.7

본 연구에서는 식물체에 널리 분포하는 indole-3-carbinol (I3C)에 의한 OVCAR-3 인체 난소암세포의 이동성 및 침윤성 억제 가능성과 이와 연관된 기전을 조사하였다. 본 연구의 결과에 의하면 I3C에 의한 OVCAR-3 세포의 증식억제는 세포의 이동성 억제와 연관이 있었으며, 이를 wound healing 및 matrigel invasion assay로 확인 하였다. 아울러 I3C 처리에 의하여 transepithelial electrical resistance가 증가되었으며, cellular paracellular permeability는 감소되었는데, 이는 I3C 처리에 의해 세포 내 치밀결합(tight junctions, TJs)의 tightness가 증가되었음을 의미한다. RT-PCR 및 immunoblotting 결과에 의하면, I3C는 TJs의 구성 성분이면서 paracellular transport 의 선택적 투과성을 조절하는 주요 인자인 claudin-3 및 -4의 발현을 유의적으로 억제하였다. 또한 matrix metalloproteinase (MMP)-2 및 -9의 활성이 I3C 처리에 의하여 매우 억제되었는데, 이는 그들의 mRNA 및 단백질 수준에서의 발현 감소와 연관성이 있었다. 따라서 I3C에 의한 OVCAR-3 난소암세포의 침윤성 억제는 TJs 기능의 강화와 MMP 활성의 저하가 주요 인자로 작용함을 알 수 있었다. In the present study, we investigated the effect of indole-3-carbinol (I3C), a natural compound present in vegetables, on the cell migration and invasion of OVCAR-3 ovarian cancer cells. Our results indicated that I3C inhibited the proliferation of OVCAR-3 cells, a process which was associated with inhibition of cell motility as determined by wound healing experiments and cell invasion studies. I3C treatment increased the tightness of the tight junctions (TJs), which was demonstrated by an increase in transepithelial electrical resistance and a decrease in paracellular permeability. The RT-PCR and immunoblotting results indicated that I3C repressed the levels of claudin-3 as well as claudin-4, proteins that comprise a major part of TJs and play a key role in the control and selectivity of paracellular transport. Furthermore, the activities of matrix metalloproteinase (MMP)-2 and MMP-9 were also decreased by treatment with I3C, which was connected with the down-regulation of their mRNAs and protein expression. The results suggest that I3C may be expected to inhibit cancer cell metastasis and invasion by restoring TJs and decreasing MMP activity in ovarian cancer cell line OVCAR-3.