2시간 허혈 후 재관류 시간 경과에 따라 나타나는 혈청 사이토카인의 발현에 미치는 허혈양상화의 효과

진정한(Jeong han Jin),김성재(Sung Jae Kim),서윤경(Younkyoung Seo),김신영(Shinyoung Kim) 대한체질인류학회 2016 대한체질인류학회지 Vol.29 No.2

일시적인 허혈과 수술로 인한 허혈 후 재관류 시 대량의 활성산소가 발생하여 세포에 손상이 발생한다. 이러한 허혈-재관류 손상으로 인해 국소적 손상이 초래되고, 전신적 손상이 발생하기도 한다. 따라서 허혈-재관류 손상을 감소시키기 위한 필요성이 대두되었고 이와 관련해서 허혈 전에 짧은 허혈과 재관류를 반복하는 허혈양상화에 대한 연구가 진행되었다. 허혈양상화는 허혈시 염증반응을 감소시킴으로 허혈 손상에 대해 조직을 보호한다고 알려져 있다. 이에 본 연구에서는 세포조직이 허혈과 재관류 손상에 대해 내성의 기전을 갖게 하면서 조직을 보호한다고 알려진 허혈양상화를 기본으로 하였고, 허혈-재관류 손상에서 나타나는 염증반응에서 중요한 역할을 하는 물질이 사이토카인이기 때문에, 재관류 시간에 허혈양상화 방법론 고찰과 허혈양상화가 사이토카인 발현에 미치는 영향에 대한 상관관계를 분석하였다. 40~45 g 수컷 생쥐의 왼온엉덩동맥을 허혈처치 하였으며 2시간 허혈처치만 시행한 허혈군, 5분간의 짧은 허혈과 재관류를 3회 처치한 허혈양상화군, 허혈양상화 처치 후 2시간 허혈을 시행한 허혈양상화 및 허혈군으로 하는 실험군을 구성하였고 각 실험군에서의 처치 후 비교를 위한 재관류 시간은 30분, 1시간, 2시간, 4시간을 시행하였다. 연구결과는 다음과 같다. 첫째, 허혈군이 허혈양상화군과 허혈양상화 및 허혈군과 비교했을 때 친염증성 사이토카인 IL-1β의 수치가 가장 높게 발현되었다 (p<.001). 둘째, 항염증성 사이토카인인 IL-4는 허혈군, 허혈양상화 및 허혈군과 비교했을 때 허혈양상화군에서 가장 높게 발현되었다 (p<.001). 셋째, 항염증성 사이토카인인 IL-10은 허혈군, 허혈양상화 및 허혈군과 비교했을 때 허혈양상화군에서 가장 높게 발현되었다 (p<.001). 위의 결과들을 종합했을 때 정도에 따라 차이는 있지만, 허혈양상화 처치는 허혈군과 허혈양상화군보다 허혈양상화 및 허혈군의 친염증성 사이토카인 IL-1β를 감소시켰고, 항염증성 사이토카인 IL-4와 IL-10은 허혈양상화군에서 허혈군과 허혈양상화 및 허혈군보다 증가하였다. The large volume of reactive oxygen species are generated during reperfusion after transient or postprocedural ischemia, which leads to cell injury. This ischemia-reperfusion (IR) injury may cause local and even systemic injuries. Thus, the need to reduce the IR injury has been highlighted and in this regard studies have demonstrated the ischemic preconditioning (IP) in which short ischemia and reperfusion are repeated before ischemia. Such IP is known to protect the tissues from IR injury by reducing inflammation response during ischemia. Thus, this study was based on IP known to protect the tissue with developing the mechanism of resistance to ischemia and reperfusion injury in cellular tissue. As the substance that plays an important role in the inflammatory response during IR injury is cytokines, this study was intended to review and discuss the methodologies of IP as well as to analyze the correlation of its effects on the expression of cytokines. Left common iliac artery in male mice of which weight was from 40 g to 45 g, was treated for ischemia. The animal groups consisted of ischemia (IC) group receiving 2-hour ischemic treatment alone; IP group receiving short 5-minute ischemia and reperfusion treatments repeated three times; and, ischemic preconditioning-ischemia (IP-IC) group receiving IP treatment followed by 2-hour ischemic treatment. Following these treatments in each group, reperfusion for intergroup comparisons was carried out at 30 minutes, and 1, 2 and 4 hours. The results of this study were as follows: First, the expression level of pro-inflammatory cytokines, IL-1β was the highest in IC group receiving 2-hour ischemic treatment alone (p<.001). Second, the expression level of anti-inflammatory cytokine, IL-4 was the highest in the IP group (p<.001). Third, the expression level of anti-inflammatory cytokine, IL-10 was the highest in the IP group (p<.001). In conclusion, even though the results had the degree of difference, the expression level of pro-inflammation cytokine, IL-1β in IC group was significantly lower than that in IP group, and the expression levels of antiinflammatory cytokines, IL-4 and IL-10 in IP were significantly higher than those in IC group and IP-IC group.

스마트 건설현장을 위한 Ex-BIM 추진 및 시공단계 매뉴얼 제작

진정한(Jin Jeong han),왕명훈(Wang Myung hoon),심창수(Shim Chang Su),강전용(Kang Jeon Yong) 한국BIM학회 2018 KIBIM Magazine Vol.8 No.4

차세대 첨단 고속도로 구축을 위한 Ex-BIM 적용 현황 및 향후 전망

진정한,왕명훈,심창수,Jin, Jeong-Han,Wang, Myeong-Hun,Sim, Chang-Su 한국건설관리학회 2018 한국건설관리학회 논문집 Vol.19 No.1

민자 고속도로 무정차 통행료 시스템 설치시 기존 영업소 철거 방법론 연구

진정한,고민기,백현수,유주성,Jin, Jeong-Han,Go, Min-Gi,Baek, Hyeon-Su,Yu, Ju-Seong 대한교통학회 2016 교통 기술과 정책 Vol.13 No.6

황색종 연초 황변기 cyclic건조가 건조엽의 물리성에 미치는 영향

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1998 한국연초학회지 Vol.20 No.1

In the flue curing process, the wet bulb temperature is usually controlled at a constant level. To improve the quality of flue cured leaves, we studied the effect of the cyclic change of wet bulb temperature at the yellowing stage of flue curing on physical properties of cured leaves. The wet bulb temperature was automatically controlled between preset high (38℃) and low point (35℃) every one hour cyclically. As a result, the acceleration of the increase in the physical properties of cured leaves were observed. The leaves cured by this method were more or less orange in color, better bodied, and less brittle compared with the leaves produced by conventional curing. However, the leaves cured by this method had a little sharpness and harshness. As to the physical properties, there was decreased in occurrence of flat leaves than that of conventional ones. On the other hand, in case of cyclic curing method, increase of price per kg reached to 2-3% compared with those of conventional ones.

연초 III형 Grey엽의 발생양상과 화학성분 특성

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1996 한국연초학회지 Vol.18 No.2

The occurrence and chemical properties of grey tobacco leaves (Type III) found in 1995 crop of flue-cured tobacco cultivated in paddy field were investigated to compare with those of normal leaves. Cured leaves of NC82 were separated by visual characters into 3 classes of slight, fair and severe symptoms of grey leaf by the percentage of grey parts to whole leaf area. Number of samples classified with discoloring portion was the order of greyish brown > reddish brown > greyish yellow, respectively. Grey leaves of this type were mostly found among the leaves harvested from upper stalk position, and it was estimated that growth rate of upper leaves also influenced on the occurrence of grey leaves. Grey leaves showed remarkably lower b* and L* values than those of normal leaves, while a* value was mostly higher in grey leaves. These tendencies in chromatic aberration showed more remarkable difference in the degree of grey symptoms. Chemical analyses of grey leaf samples indicated that they contained less total nitrogen and nicotine, and more total sugar and starch than those of normal leaves. In chemical traits, these tendencies were accordance with the degree of grey symptoms, and within the same leaf, grey parts were decreased in total nitrogen and ether extract content compared with those of normal parts, but there was no difference in nicotine and Cl contents. Key words : grey leaf tobacco (type III), grey symptom, color, chemical properties.

황색종 cyclic 건조엽의 화학성분 특성

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1998 한국연초학회지 Vol.20 No.1

The bulk curing experiment to the improve the quality of flue-cured leaves were carried out to evaluate relationship between cyclic curing and conventional curing method. We studied the effect of the cyclic change of wet bulb temperature at the yellowing stage of flue curing on chemical properties of cured leaves. The wet bulb temperature was automatically controlled between preset high($38^{\circ}C$) and low point ($35^{\circ}C$) everyone hour cyclically during 12 hours after starting luring. As a result, the acceleration of the increase in the chemical properties of cured leaves were observed. As to the chemical properties, there was decreased in citric acid, increased in malic acid of the nonvolatile organic acids and all higher fatty acids content of leaves cured by this method compared with in that of conventional curing method, while a major chemical compounds in relation to aromatic essence of cigarette smoke in essential oil components of lured leaves was mostly higher in this method than those of conventional ones, and it was evaluated that there was decreased in CO, TPM, Tar, and $CO_2$ content of the cigarette smoke comparing to the conventional luring method.

연초 수확엽의 숙도가 색채 및 색소물질 함량에 미치는 영향

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1996 한국연초학회지 Vol.18 No.1

Field experiment was conducted to get the basic information about the difference in color and plastid pigments at the different stage of harvest of flue-cured tobacco cultivated in paddy field, and compared to upland ones. All tested crops were primed in lots of 3 to 4 at each priming, beginning at the bottom of the plant. Leaf pigments and chromatic values of tobacco leaves were determined in samples collected at weekly intervals, and according to analyses obtained from 4 stalk positions. By the degree of maturity, leaves harvested were separated with visual characters into four classes of immature, mature, ripe and mellow. regardless of stalk position, chlorophyll contents was higher in the order of immature > mature > ripe > mellow, respectively. In fresh leaves, chlorophyll levels was higher with ascending stalk position. chlorophyll a and b in cured leaves were less than 10% of the amount present at harvest, and it was estimated that maturity condition also influenced the rates of degradation of plastid pigments. Fully mature leaves of upland diverted from paddy field showed slightly higher in chlorophyll contents than in those of upland tobacco ones but somewhat lowered in $L^*$ and $b^*$ values, and there was no difference in $a^*$ value.

유선망 SMS 서버 및 망 구축 기술

배정일(Jeong-Il Bae),한동영(Dong-Young Han),이동수(Dong-su Lee),진정학(Jeong-Hak Jin),김정식(Jeong-Shik Kim) 한국정보통신설비학회 2002 한국정보통신설비학회 학술대회 Vol.2002 No.1

사료작물 윤작재배에 의한 연초포지의 제염효과

이철환,진정의,한철수,Lee, Chul-Hwan,Jin, Jeong-Eui,Han, Chul-Soo 한국연초학회 1997 한국연초학회지 Vol.19 No.1

The field experiment for field desalinization by precultivation of orchard crops were carried out to evaluate relationship between the varieation of chlorine contents of soil and crop uptake in the upland diverted from paddy field. After harvest of grass crops, soil samples were taken for analysis of chlorine contents of soil layers. Regardless of kinds of grass crops cutivated, contents of soil chlorine were decreased comparing to non-crop plot. Chlorine content in plant harvested at just before the flowering stage was much higher than that of after flowering. Chlorine uptake and dry matter were increased in order of Italian ryegrass, Perennial ryegrass, and Sudan grass. Positive correlations were showed between chlorine uptake and dry matter. The content of soil chlorine decreased by higher yield of dry matter.