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      • KCI등재

        가락지나물의 생장과 생리활성물질 함량에 미치는 차광, 광질 및 화학적 엘리시테이션

        이종두,박정애,박병준,정철승,박소영,백기업 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.4

        Potentilla kleiniana is a perennial herb beloning to Rosaceae family. Herein we investigated the effect of light intensity, light quality and chemical elicitor on plant growth and the accumulation of bioactive compounds in P. kleiniana. After 60 days of cultivation under different shading level [0% (200 μmol·m -2 ·s -1 ), 35% (95 μmol·m -2 ·s -1 ), 55% (65 μmol·m -2 ·s -1 ), 75% (40 μmol·m -2 ·s -1 )] in the greenhouse, chlorophyll and carotenoid content were the highest under 35% treatment, however, plant height, leaf number and biomass were the highest under non-shading. As a result of cultivation among strong light condition as a control, florescence and three mixture light sources [red:white:blue (RWB) = 8:1:1, red:blue (RB) = 8:2, red:green:blue (RGB) = 8:1:1] as treatments in plant growth chamber (25 ± 2℃, 185 ± 3 μmol·m -2 ·s -1 ), growth, biomass, chlorophyll content low difference between total phenolic compouds and flavonoid content were higher under RWB treatment. DPPH radical elimination ability was the highest under all treatments especially florescence and RGB treatment except control. As a result of treating chemical elicitor [salicylic acid (SA), methyl jasmonate (MeJA)] concentration (0, 50, 100, 200 μM) respectively, plant height, petiole diameter and biomass were higher under non-treatment, MeJA 50 μM. It was investigated that fresh weight and dry weight under MeJA 50 μM treatment were especially a little high. Total phenolic compounds and flavonoid content of SA 50 μM treatment was the highest but DPPH radical elimination ability was significantly the highest under MeJA 200 μM (88.65%) and MeJA 50 μM (87.84%) treatment. Thus, this study suggested that we determined optimal shading and light quality in the greenhouse and plant growth chamber also confirmed bioactive compound content, antioxidant ratio increase according to different chemical elicitation concentration.

      • KCI등재

        추출조건이 노니 부정근에 함유된 rubiadin의 추출 효율에 미치는 영향

        김명기(Myong-Ki Kim),정철승(Cheol-Seung Jeong),신용국(Yong-Kook Shin),박경희(Kyong-Hee Park),이운장(Woon-Jang Lee),이은정(Eun-Jung Lee),백기엽(Kee-Yoeup Paek) 한국원예학회 2010 원예과학기술지 Vol.28 No.4

        노니(Morinda citrifolia) 부정근의 주요 성분인 rubiadin은 간 보호 효과가 있어 제약산업에서 높은 가치가 있다. 노니 부정근의 주요 유효성분인 rubiadin의 효율적인 추출조건을 규명하고자 용매 종류, 물과 메탄올의 비율(물, 20%, 40%, 60%, 80%, 100%), 추출시간 및 추출방법을 달리하여 추출하였다. 노니 부정근에서의 rubiadin의 함량분석은 HPLC이용 분석조건을 확립하였으며, C-18 컬럼을 사용하여 280nm에서 메탄올과 물로 농도구배를 주어 분석하였다. 용매별추출효율은 메탄올(0.08%)>에탄올(0.05%)>아세토니트릴(0.03%)>아세톤(0.02%) 및 메틸렌클로라이드(0.02%)순으로 증가하였다. 메탄올에 물의 혼합 비율을 달리하여 초음파 추출기로 1시간 동안 추출한 결과 60% 메탄올(0.21%)>80% 메탄올(0.13%)>100% 메탄올(0.07%) 및 40% 메탄올(0.07%) 순으로 효과적이었으며, 환류냉각 추출기로 2시간 동안 추출한 결과 60% 메탄올(0.21%)>40% 메탄올(0.17%)>80% 메탄올(0.14%) 용매처리구 순으로 효율이 좋았다. 추출방법 및 추출시간에 따른 rubiadin의 추출 효율을 비교하기 위해 환류냉각추출 및 초음파추출, 진탕추출법으로 추출하였다. 추출방법 및 추출시간에 따른 rubiadin의 추출효율은 초음파추출 및 진탕추출에서 각각 8 및 24시간동안 추출했을 때 효율적이었다. Rubiadin, a major compound of noni (Morinda citrifolia) adventitious root, is highly valued in pharmaceutical industry due to hepatoprotective activity. To dissect rubiadin’s effective extraction condition, extraction process of noni adventitious roots was performed with different solvent types, ratio of water to methanol (water, 20, 40, 60, 80, and 100% of methanol), extraction time, and extraction method. In contrast, we also developed a reverse- phase HPLC assay method to determine rubiadin from noni adventitious roots. The HPLC assay of rubiadin was performed by C-18 column using a gradient solvent system of methanol and water with UV detector at 280 ㎚. The extraction efficiency of different types of solvents were increased in order of methanol (0.08%)>ethanol (0.05%)>acetonitrile (0.03%)>acetone (0.02%) and methylene chloride (0.02%). The results of rubiadin extraction using different solvents showed that 1 hour of ultrasonic extraction was effective in order of 60% methanol (0.21%)>80% methanol (0.13%)>100% methanol (0.07%), 40% methanol (0.07%) and 2 hours of reflux extraction was effective in order of 60% methanol (0.21%)>40% methanol (0.17%)>80% methanol (0.14%). To compare the extraction efficiency of rubiadin according to the extraction methods and time for high rubiadin content, the extracts of rubiadin in noni adventitious roots were isolated with the methods of ultrasonic extraction, shaking extraction and reflux extraction. Rubiadin extracted from the methods of ultrasonic waves and shaking displayed the highest contents at 8 and 24 hours, respectively.

      • KCI등재

        유동층 공정을 이용한 열화학적 전환 공정의 최신 개발 동향

        박현준,오승석,Ogunsola Nafiu Olanrewaju,Jester Lih Jie Ling,정철승,박한샘,이시훈 한국화학공학회 2023 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.61 No.1

        Increasing of energy demand due to the rapid growth of global population and the development of world economy has inevitably resulted in the continuously increase of fossil fuel usage in the world. However, highly dependence on fossil fuels has necessarily brought about critical environmental issues and challenges such as severe air pollutions and rapid global warming. In order to settle these environmental and energy problems, clean energy generations in the conventional combustion processes have widely adapted in the world. In particular, novel thermochemical conversion processes such as pyrolysis and gasification have rapidly been applied for generating clean energy. Fluidized bed technologies having advantages such as various fuel use, easy continuous operation, high heat and material transfer, isothermal operation, and lower operation temperature are widely adopted and used because they are suitable for thermochemical energy conversion. The latest research trends and important findings in the thermo-chemical conversion process with fluidized bed technologies are summarized in this review. Also, the need for research such as layered materials and substances to reduce fine dust (biomass, natural resource waste, etc.) was suggested. Through this, it is intended to increase interest and understanding in fluidized bed technology and to present directions for solving future challenges in fluidized bed process technology development.

      • KCI등재

        접시꽃 캘러스 추출물의 천연화장품 원료로서의 효능 평가

        이기복 ( Gibok Lee ),염아름 ( Areum Yeom ),김동원 ( Kim Dong Won ),박창민 ( Chang-min Park ),정민석 ( Min-seok Joung ),이기용 ( Gi Yong Lee ),정철승 ( Cheol-seung Jeong ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.3

        본 연구에서는 천연 화장품 소재 개발을 위하여 접시꽃 캘러스 생리활성 효능에 대해서 평가하였다. 접시꽃캘러스 추출물의 항산화 효능은 DPPH, ABTS, FRAP assay를 통해서 확인하였다. 그 결과 접시꽃캘러스 추출물은 농도 의존적으로 강력한 항산화 능력을 확인되었다. 더불어, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 세포 내 ROS를 효과적으로 감소시키는 것으로 확인되었다. 접시꽃 캘러스 추출물의 미백효능을 평가하기 위해 tyrosinase 저해 효과를 확인한 결과, 접시꽃 캘러스 추출물은 10 mg/mL 의 농도에서 tyrosinase 활성을 51% 감소시키는 것으로 확인되었다. 이상의 결과를 바탕으로, 접시꽃 캘러스 추출물은 항산화 및 미백 효능을 지닌 화장품 기능성 소재로서 적용 가치가 높은 천연소재로 사료된다. In this study, we investigated the biological effects of Alcea rosea L. callus extract for the development of natural cosmetics ingredients. The antioxidant activities of A. rosea L. callus extract was measured through DPPH, ABTS and FRAP assay. As a result, A. rosea L. callus extract were found to have a strong antioxidant ability in a dose dependent manner. In addition, A. rosea L. callus effectively reduced the intracellular oxidative stress induced by AAPH at a concentration of 10 mg/mL. In a tyrosinase activity assay, we found that A. rosea L. callus extract reduced tyrosinase activity by 51% at 10 mg/mL. Based on these results, A. rosea L. callus extract is considered as a promising natural ingredients for cosmetics with antioxidant and whitening functions.

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