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만성 HBsAg보유자에서 중합효소 연쇄반응법에 의한 혈청 HBV DNA의 검출
정일권(Il Kwun Jung),주재학(Jae Hak Joo),차상우(Sang Woo Cha),봉형근(Hyung Keun Bong),김진오(Jin Oh Kim),박찬욱(Chan Wook Park),이준성(Joon Seong Lee),이문성(Moon Sung Lee),조성원(Sung Won Cho),심찬섭(Chan Sup Shim) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.6
N/A Background/Aims: The purpose of this study was to investigate the serum HBV DNA status and clinical significance of polymerase chain reaction(PCR) in patients with chronic hepatitis B and asymptomatic HBsAg carriers. Methods: The subjects of this study were 80 patients with chronic hepatitis B(53 HBeAg posiiive patients, 27 HBeAg negative patients), 19 asymptomatic HBsAg carriers(8 HBeAg positive patients, l l HBeAg negative patients). Serum HBV DNA was measured by Dot blot hybrydization with a ' P labeled HBV DNA in all patients studied, and the PCR was used to detect serum HBV DNA in Dot blot negative patients. The PCR was performed with 2 primers in the C gene region of HBV. Amplified sequences were detected with ethidium bromide staining(PCR-EB) and southern blot hybridization(PCR-SBH). Dot blot allowed the detection of 10 ' pg of cloned HBV DNA, while 10 pg and 10' pg were detected by PCR-EB and PCR-SBH, respectively. Results: In the 80 patients with chronic hepatitis, 76patients(95%) were positive for HBV DNA by Dot blot or PCR. All 53 HBeAg positive chronic hepatitis patients had HBV DNA detectable by Dot blot(77.4%) or PCR(18.97c by PCR-EB, 3.7% by PCR-SBH). Out of 27 HBeAg negative patients, 23(85.2%) were positive for HBV DNA by Dot blot (40.8/o) or PCR (29.61o by PCR-EB, 14,8% by PCR-SBH). In the 19 asymptomatic HBsAg carriers, 13(68.4%) were positive for HBV DNA by Dot blot or PCR. All 8 HBeAg positive asymptomatic carriers had sufficient HBV DNA detectable by Dot blot. Out of 1 l HBeAg negative carriers, 5(45.5 7c) were positive for HBV DNA by Dot blot (9.1%) or PCR(36.4%). The positive rate for HBV DNA in patients with chronic hepatitis was significantly higher than that in asymptomatic HBsAg carriers(P=0.002 by Fisher exact test) and there is a significant difference in serum HBV DNA between HBeAg positive and negative asymtomatic HBsAg carriers(P=0.02 by Fisher exact test). Conclusions: These results suggest that minute amount of HBV DNA detected by PCR may have pathogenic relevance in chronic hepatitis B, and PCR may be used as a prognostic factor in asymptomatic HBsAg carriers. (Korean J Gastroenterol 1995;27: 651 - 658)
볼 베어링과 형상오차를 갖는 하우징의 끼워 맞춤에 따른 베어링 진동 및 피로 수명의 영향
이영근(Lee, Young-Keun),이석훈(Lee, Seok-Hoon),정일권(Jung, Il-Kwon),차철환(Cha, Cheol-Hwan),한효섭(Han, Hyo-Seup) 한국소음진동공학회 2006 한국소음진동공학회 논문집 Vol.16 No.5
It is known that ball bearings mounted in housing or on shaft are playing a key role to keep it running smoothly. The roundness of a housing bore on which bearing outer ring is mounted with interference has directly affected the running accuracy of bearing. The running accuracy of bearing, therefore, can extend the significant influence to the rotating machinery as well. In this paper, bearing's vibration and fatigue life considering raceway roundness of ball bearing before and after mounted in housing bore are theoretically estimated. To perform analysis, a simple three degrees of freedom model was proposed and then Newton-Raphson iterative method was introduced to be utilized in the analysis. The results show that the vibration magnitude of ball bearing fitted into housing unit is appeared considerably larger than the one of its pre-assembling. And theoretical $L_{10}$ life which ninety percent of the bearing population will endure decreased in about fifty percent.
내시경적 식도정맥류 결찰요법 ( EVL ) 이 식도운동에 미치는 영향 - 식도내압검사를 중심으로
김영석(Young Seok Kim),권강호(Kwang Ho Kwun),정일권(Il Kwun Jung),이은학(Eun Hag Lee),봉형근(Hyung Keun Bong),송동화(Dong Hwa Song),박찬욱(Chan Wook Park),이준성(Joon Seong Lee),이문성(Moon Sung Lee),조성원(Sung Won Cho),심찬섭(Chan 대한소화기학회 1995 대한소화기학회지 Vol.27 No.1
N/A Background/Aims: For treating and eradicating bleeding esophageal varices, endoscopic variceal ligation(EVL) is currently acceptable practiced method, however esophageal motility change associated with endoscopic variceal ligation is uncertain. Although Longterm follow-up for rebleeding rate, recurrence rate, complication and survival rate after endoscopic variceal ligation are reported, there is a few report of esophageal motility change after endoscopic variceal ligation. To assess the effect of endoscopic variceal ligation on esophageal function prospectively, parameters measured by pneumohydraulic capillary infusion system, included lower esophageal sphincter(LES) pressure, % LES relaxation, amplitude, duration, velocity and progression of peristaltic waves and dysmotility, were compaired hetween before and after endoscopic variceal ligation in 12 patients with csophngeal varix. Results: Endoscopic variceal ligation has no impact on lower Esophageal sphincter(LES) tone. However, a significant increase in the amplitude of peristaltic waves was observed in the patients at'ter endoscopic variceal ligation in the mid-esophagus(8cm above lower esophageal sphincter: 57.2 ' 19.4 versus 65.7 ' 20.3, l3cm above lower esophageal sphincter; 5S,7 ' 23.8 versus 62.l ' 2I.S). There was no modification of duration, velocity and progression of peristaltic waves or dysmotility. Conclusions: Endoscopic variceal ligation is associated with a increasing esophageal body pressure without the other esophageal motility change. (Korean J Gastroenterol 1995;27:9 -17)