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정성근 ( Sung Keun Jung ),이형주 ( Hyong Joo Lee ) 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.2
본 연구에서는 강원도에 자생하는 오가피(Acanthopanax sessilifloru) 열매(Ogaza)를 80% MeOH 추출하고, 기능성식품소재로써의 기능성을 규명하였다. 오가자 추출물의 페놀성 화합물은 오가자 1 g 당 56.1±5.2mg 의 gallic acid로 나타났다. 오가자 추출물 0.25, 0.5, 1, 2mg/mL은 각각 vitamin C 34.0, 73.0, 194.3, 339.7μg/mL에 해당하는 항산화 효과를 보였다. 오가자 추출물 1 과 2mg/mL의 농도에서 LPS로 유도한 TNF-α와 IL-6의 생성량을 각각 22±2, 19±6% 그리고 18±2, 24±3% 억제하였다. 또한, 자외선 조사에 의해 증가한 COX-2 luciferase 활성이 오가자 추출물(0.25, 0.5, 1, 2mg/mL)에 의해 각각 80±1, 83.±7, 96±4, 98±2% 억제됨을 확인하였다. 본 연구를 통해 잎 이나 뿌리에 비해 연구 및 활용도가 낮았던 오가피 열매의 항산화활성 및 항염증 효능을 규명함으로써, 오가자가 고부가가치기능성 식품소재로 활용 될 수 있는 가능성을 제시하였다. Multiple lines of study have shown that Acanthopanax species have anti-oxidant and chemopreventive effect. However, the suitability of Acanthopanax sessilifloru fruit (Ogaza) as a functional food source remains to be investigated. Therefore, we have investigated the effect of Ogaza as an anti-oxidant and anti-inflammatory substance. The phenolic content of Ogaza is 56.1±5.2mg gallic acid equivalents (GAE) per 1 g of Ogaza. The 2,2`-azino-bis(3-ethylbenzthiazoline- 6-sulphonic acid (ABTS) radical scavenging effects of Ogaza extract at 0.25, 0.5, 1, or 2mg/mL were 34.0, 73.0, 194.3, or 339.7μg/mL vitamin C equivalent antioxidnat capacity (VCEAC), respectively. Ogaza extract (1 or 2 mg/mL) inhibited LPS-induced TNF-a production (decrease of 22±2% or 19±6%, respectively). It also inhibited LPS-induced IL-6 production (decrease of 18±2% or 24±3%, respectively). In addition, Ogaza extract (0.25, 0.5, 1, or 2mg/mL) inhibited COX-2 luciferase activity (decrease of 80±1%, 83.±7%, 96±4%, or 98±2%, respectively). Overall, these results indicated that Ogaza is promising as a functional food source due to its antioxidant and anti-inflammatory effects.
제주 자생식물로부터 항산화 및 화장품 기능성 소재 탐색
현선희(Sun-Hee Hyun),정성근(Sung-Keun Jung),죄미경(Mi-Kyung Jwa),송창길(Chang-Khil Song),김지훈(Ji-Hun Kim),임상빈(Sangbin Lim) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.2
제주 자생식물 54종을 대상으로 70% 메탄올로 추출하여, 총페놀 함량, DPPH radical 소거능, xanthine oxidase(XOD), tyrosinase 저해활성을 측정한 후, 생리활성이 높은 5가지 식물을 선정하여 고압 유기용매(100% 메탄올, 13.6㎫, 40℃)로 추출하여 DPPH, tyrosinas, elastase 저해활성을 측정하였다. 70% 메탄올 추출물의 총페놀 함량은 새우나무, 이질풀, 아그배나무, 자금우, 짚신나물이 250㎎ GAE/g 이상으로 높은 함량을 나타내었다. DPPH radical 소거활성은 백량금이 94.1%로 가장 높았다. DPPH radical 소거활성과 총페놀 함량 사이에는 높은 상관관계(R²=0.87)를 나타내었다. Tyrosinase 저해활성은 이삭여뀌, 붉나무, 사방오리나무, 소귀나무가 85% 이상을 나타내었으며, XOD 저해활성은 이삭여뀌와 소귀나무가 90% 이상을 나타내었다. 고압 유기용매 추출물은 70% 메탄올 추출물과 마찬가지로 DPPH 소거능과 tyrosinase 저해활성이 높았다. Tyrosinase와 elastase의 저해활성에 대한 IC??은 자금우가 각각 802와 88 ppm이었고, 소귀나무가 각각 959와 66 ppm이었다. Fifty-four natural plants from Jeju Island, Korea were extracted by 70% methanol. The extracts containing the highest total phenolic contents (TPC) (>250 ㎎ gallic acid equivalents/g dry sample) were obtained from Ostrya japonica, Geranium thunbergii, Malus sieboldii, Ardisia japonica, and Agrimonia pilosa. DPPH inhibition activity was greatest in Ardisia crenata at 94.1%. A high correlation was observed between DPPII inhibition activity and TPC (R²=0.87). Tyrosinase inhibition activities of more than 85% were obtained from the extracts of Persicaria filiformis, Rhus javanica, Alnus firma, and Myrica rubra. On the other hand, the P. filiformis and M. rubra extracts each showed more than 90% XOD inhibition activity. The five natural plants with the highest biological activities were also extracted by pressurized liquid (PLE, 100% methanol, 13.6 ㎫, 40℃). The DPPH and tyrosinase inhibition activities were almost the same in both the 70% methanol and PLE extracts. The IC?? for tyrosinase and elastase inhibition activities in PLE were 802 and 88 ppm in A. japonica, and 959 and 66 ppm in M. rubra, respectively.
전통 발효 식품에서 분리한 신규 젖산균의 프로바이오틱스 특성 및 생리활성 평가
김지혜(Ji-Hye Kim),정성근(Sung-Keun Jung),조영제(Young-Je Cho),김병오(Byung-Oh Kim) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.5
This study evaluated the characteristics of lactic acid bacteria (LAB) isolated from kkakdugi for its use as a probiotic. In addition, the possibility of using it as a material for promoting antioxidant activity and skin functionality was evaluated. To verify the feasibility of LAB as probiotics, their survival rates in artificial gastric juice and artificial bile were evaluated. In artificial gastric juice, the average number of probiotics was maintained at 5.3×109 colony-forming units (CFU)/mL, showing a survival rate of about 99%. In artificial bile, the average number of probiotics was maintained at 1.2×109 CFU/mL, showing a survival rate of about 95%. The survival rate indicated their ability to reach the target site to exert their effects. In addition, autoaggregation and cell surface hydrophobicity experiments were conducted to indirectly confirm their ability to adhere to the gastrointestinal tract surface. The autoaggregation rate of all LAB strains increased over time. Specifically, L. plantarum K1-9 and L. brevis K2-9 strains showed high hydrophobicity. LAB culture supernatants were used to evaluate antioxidant activity, antidiabetic activity, and skin functionality, such as the effects on skin wrinkles, whitening, and astringent effects. The results showed that, the LAB isolated from kkakdugi had high antioxidant activity. In addition, increasing the concentration of the LAB culture supernatant is expected to have positive effects on skin wrinkles and whitening. Therefore, it is believed that these characteristics of the LAB isolated from kkakdugi ensure that they have sufficient potential to be utilized as an intestinal probiotic and functional cosmetic materials.
신규 젖산균을 활용한 Anti-Helicobacter pylori 활성
김지혜(Ji-Hye Kim),정성근(Sung-Keun Jung),조영제(Young-Je Cho),김병오(Byung-Oh Kim) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.5
In this study, we aimed to isolate and identify strains of lactic acid bacteria from kkakdugi, to validate their potential use as alternative therapeutic agents against Helicobacter pylori. Eleven strains were identified, including Lactobacillus plantarum, Pediococcus pentosaceus, Lactobacillus brevis, Pediococcus acidilacti, and Leuconostoc mesenteroides. The paper disk method and urease inhibition activity were used to verify anti-H. pylori activity. Without pH adjustment, most strains exhibited an average inhibitory zone of 13.68 mm. However, when the pH was adjusted to 7.0, some strains showed inhibitory zones ranging from 11.65 to 13.15 mm. Nevertheless, upon comparison, it was observed that the antimicrobial activity was higher when the pH was not adjusted. On the other hand, antimicrobial activity against H. pylori G88026 strain was exhibited regardless of the pH. The results of urease inhibition confirmed a significant reduction of approximately 60∼90% in urease activity when the lactic acid bacterial culture supernatant was added. Except for the Lactobacillus sakei strain, the remaining strains exhibited potent urease inhibition activity. This suggests that the lactic acid bacteria isolated in this study could be promising candidates as alternative therapeutic agents against H. pylori.