상황버섯, 차가버섯, 느타리버섯 발효식초의 항산화와 항암활성 효과

정봉환,서훈석,김홍식,우선희,조용구 한국약용작물학회 2010 한국약용작물학회지 Vol.18 No.2

The antioxidant and anticancer activities of Phellinus linteus, Inonotus obliquus, and Pleurotus ostreatus according to extraction methods were measured. The contents of polyphenolic compounds were higher in fermented extracts than those in water extracts. The contents were 192.4mg/100 ml for Inonotus obliquus vinegar, 186.9 mg/100 ml for Phellinus linteus vinegar, and 156.5 mg/100 ml for Pleurotus ostreatus vinegar, respectively. EDA(electron donating ability) in Phellinus linteus that was highest among mushrooms was 83.9% in the water extract and 96.8% in the fermented extract. The growth of stomach cancer cells, SNU-719 was inhibited 62.4%, 65.5%, and 53.0% by Phellinus linteus vinegar, Inonotus obliquus vinegar, and Pleurotus ostreatus vinegar, respectively and also the growth of liver cancer cells, Hep3B was inhibited 67.1%, 68.3%, and 57.6% by Phellinus linteus vinegar, Inonotus obliquus vinegar, and Pleurotus ostreatus vinegar, while the growth of normal cell, DC2.4 was not affected. Even though Phellinus linteus and Inonotus obliquus showed higher inhibition in cancer cell growth, Pleurotus ostreatus can be efficiently used for antioxidant and anticancer activities due to their cheap price in the market.

수집 선발한 쑥으로 만든 쑥차와 시판차의 항산화력 비교

정봉환,조용구 韓國作物學會 2006 Korean journal of crop science Vol.51 No.1S

전국에서 수집한 쑥들에 대한 항산화력과 항산화성분 함량을 비교 분석하여 유효 약용성분을 다량 함유하는 우량계통을 선발한 후 대량 재배하였고, 한방건강식품으로 쑥차를 개발하였다. 개발된 쑥차의 우수성을 알아보기 위해 시판되고 있는 차들을 구입하여 항산화력과 항산화성분 함량을 비교 분석하는 실험들을 수행하였다. 수집 선발한 쑥 AC60, AC67, AC77을 원료로 하여 만든 차들과 시판 차들의 총 폴리페놀 함량들을 분석한 결과 AC67의 총 폴리페놀 함량이 109.6 mg/100 mL로 가장 높았다. 선발 쑥차들의 아질산염 소거능은 선발 쑥 중에서는 AC67가 PH 1.2에서 92.5%로 가장 높았고, 시판 차들 중에는 쟈스민이 98.1%, 감잎차가 96.4%, 녹차가 98.5%, 작설차가 98.7%로 매우 높은 값들을 보여 주었다. SOD 유사 활성을 비교해 본 결과 선발 쑥을 원료로 하는 차들에서는 AC67가 91.7%, AC77이 93.7%로 높았고, 시판 차들에서는 설록차가 92.1%, 루이보스티가 93.8%로 높게 나왔다. 이 결과는 선발 쑥 AC60, AC67, AC77 등은 시판 중인 설록차, 자스민, 작설차, 녹차, 루이보스티 및 페퍼민트차 등과 같은 수준의 고급차의 제조에 이용성이 높을 것으로 생각되었다. Artemisia spp. collections, AC60, AC67, and AC77, that showed the high levels of antioxidant activities and had good growth characters and productivity, were cultivated for mass production. Using selected excellent ones, AC60, AC67, and AC77, after mass production, mugwort teas were made through rubbing and drying processes. Total phenolic compound content, nitrite scavenging ability, and SOD-like activity in mugwort teas and commercial teas were compared. Total phenolic compound content was the highest in AC67 with 109.6 mg/100 ml. Nitrite scavenging ability of AC67 tea was relatively high with 92.5% at pH 1.2 and Jasmine (98.1%), Persimmon Leaf Tea (96.4%), Green tea (98.5%), and Jakseolcha (98.7%) were very high as well. In SOD-like activity, mugwort teas, AC67 (91.7%) and AC77 (93.7%), were high as well as those of Seolrokcha (92.1%) and Rooibos Tea (93.8%). This suggests that AC60, AC67, and AC77 could be used fur making high quality teas as well as Seolrokcha, Jasmine, Green tea, Jakseolcha, Rooibos Tea, Peppermint Herb Tea.

수집 선발한 쑥으로 만든 쑥차와 시판차의 항산화력 비교

정봉환,조용구,Chung, Bong-Hwan,Cho, Yong-Gu 한국작물학회 2006 한국작물학회지 Vol.51 No.suppl1

전국에서 수집한 쑥들에 대한 항산화력과 항산화성분 함량을 비교 분석하여 유효 약용성분을 다량 함유하는 우량계통을 선발한 후 대량 재배하였고, 한방건강식품으로 쑥차를 개발하였다. 개발된 쑥차의 우수성을 알아보기 위해 시판되고 있는 차들을 구입하여 항산화력과 항산화성분 함량을 비교 분석하는 실험들을 수행하였다. 수집 선발한 쑥 AC60, AC67, AC77을 원료로 하여 만든 차들과 시판 차들의 총 폴리페놀 함량들을 분석한 결과 AC67의 총 폴리페놀 함량이 109.6 mg/100 mL로 가장 높았다. 선발 쑥차들의 아질산염 소거능은 선발 쑥 중에서는 AC67가 PH 1.2에서 92.5%로 가장 높았고, 시판 차들 중에는 쟈스민이 98.1%, 감잎차가 96.4%, 녹차가 98.5%, 작설차가 98.7%로 매우 높은 값들을 보여 주었다. SOD 유사 활성을 비교해 본 결과 선발 쑥을 원료로 하는 차들에서는 AC67가 91.7%, AC77이 93.7%로 높았고, 시판 차들에서는 설록차가 92.1%, 루이보스티가 93.8%로 높게 나왔다. 이 결과는 선발 쑥 AC60, AC67, AC77 등은 시판 중인 설록차, 자스민, 작설차, 녹차, 루이보스티 및 페퍼민트차 등과 같은 수준의 고급차의 제조에 이용성이 높을 것으로 생각되었다. Artemisia spp. collections, AC60, AC67, and AC77, that showed the high levels of antioxidant activities and had good growth characters and productivity, were cultivated for mass production. Using selected excellent ones, AC60, AC67, and AC77, after mass production, mugwort teas were made through rubbing and drying processes. Total phenolic compound content, nitrite scavenging ability, and SOD-like activity in mugwort teas and commercial teas were compared. Total phenolic compound content was the highest in AC67 with 109.6 mg/100 ml. Nitrite scavenging ability of AC67 tea was relatively high with 92.5% at pH 1.2 and Jasmine (98.1%), Persimmon Leaf Tea (96.4%), Green tea (98.5%), and Jakseolcha (98.7%) were very high as well. In SOD-like activity, mugwort teas, AC67 (91.7%) and AC77 (93.7%), were high as well as those of Seolrokcha (92.1%) and Rooibos Tea (93.8%). This suggests that AC60, AC67, and AC77 could be used fur making high quality teas as well as Seolrokcha, Jasmine, Green tea, Jakseolcha, Rooibos Tea, Peppermint Herb Tea.

강조류 악시계 환경 탐사용 다관절 해저로봇 크랩스터 (CR200)

정봉환 제어로봇시스템학회 2013 제어로봇시스템학회지 Vol.19 No.3

조수간만의 차가 크고 지형이 복잡한 우리나라 서해안은 세계적으로도 보기 드문 강조류 환경이다. 잠수부의 안전을 위협하는 이러한 환경은 수중로봇의 접근도 쉽게 허락하지 않는다. 해저로봇 크랩스터는 이러한 특수한 환경을 조사하기 위해 고안된 수중보행로봇이다. 기존의 프로펠러 방식으로 달성하기 어려웠던 문제점을 크랩스터 로봇은 게나 가재와 같은 수중 생명체를 모방하여 극복하고자 했다. 크랩스터는 게나 가재의 기능을 모방함으로써 강조류 악시계 환경에서 유용한 두 가지 특징을 얻는다. 첫째는 해저에 밀착하여 자세를 제어함으로써 조류력을 이용하여 자세를 안정화시키면서 이동할 수 있다. 둘째는 조류 속에서 동요하지 않는 안정된 자세를 바탕으로 깨끗한 초음파 영상을 얻을 수 있다. 이는 강조류 환경에서 동반되는 부유물에 의한 악시계 환경을 극복할 수 있는 중요한 수단을 제공한다. 본 고에서는 이러한 개념에 따라 설계 개발된 크랩스터 CR200의 구성과 사양을 소개하고 여기에 사용된 핵심기술을 살펴본다. 또한 수행된 CR200의 시험 결과에 대해서도 요약 소개한다.

오탄당과 육탄당의 혼합용액에서 Pichia stipitis에 의한 에탄올 발효

정봉환(Bong-Hwan Chung),유연우(Yeon-Woo Ryu),서진호(Jin-Ho Seo) 한국생물공학회 1994 KSBB Journal Vol.9 No.4

장뇌삼과 재배삼의 ginsenoside 함량과 항산화활성 추정

정은미,황인국,이민경,조성구,정봉환,조숙자,이상화,이준수,정헌상 경상대학교 농업생명과학연구원 2010 농업생명과학연구 Vol.44 No.3

국산 재배삼 4년근의 뿌리, 장뇌삼 4년근과 8년근의 잎과 뿌리에 대한 물 및 80% 에탄올 추출물에 대한 구성사포닌 및 항산화 활성을 분석하였다. 재배삼 80% 에탄올 추출물에 대한 조사포닌 함량은 3.85% (d.b) 이었으며, 4년근 장뇌삼 뿌리와 잎의 조사포닌 함량은 각각 6.75 및 8.57% (d.b)이었으며, 8년근 장뇌삼은 각각 6.53 및 7.54% (d.b)이었다. 재배삼의 구성사포닌은 ginsenoside-Rh1의 함량이 6.07 mg/g으로 가장 높았고, 4년근 장뇌삼뿌리는 -Rb1이 11.63 mg/g, 잎은 -Re가 24.35 mg/g으로 가장 높았고 8년근 장뇌삼 뿌리는 -Rh1이 19.77 mg/g, 잎은 -Re가 20.43 mg/g으로 가장 높았다. 항 산화활성 (IC50값)은 삼의 종류 및 연근별로 1.84~21.88 mg/mL의 범위이었고 8년근 장뇌삼 잎 80% 에 탄올 추출물에서 가장 높았다. 총 항산화력은 5.56~20.67 mg AA eq/g의 범위를 나타내었으며, 8년근 장뇌삼 잎 증류수 추출물에서 가장 높은 값을 나타내었다. This study was conducted to investigate the antioxidant activities and ginsenoside compositions of 4-year-old cultured ginseng roots (4CGR), 4-year-old mountain ginseng roots (4MGR) and leaves (4MGL), and 8-year-old mountain ginseng roots (MGR) and leaves (8MGL). Ginseng root and leaves were extracted with water and 80% ethanol. Crude saponin content of 4CGR was 3.85% (d.b.) and the contents of 4MGR, 4MGL, 8MGR and 8MGL were 6.75, 8.57, 6.53 and 7.54% (d.b.), respectively. 4CGR showed the highest content of ginsenoside-Rh1 (6.07 mg/g), 4MGR showed the highest content of ginsenoside-Rb1 (11.63 mg/g), 4MGL showed the highest content of ginsenoside-Re (24.35 mg/g), 8MGR showed the highest content of ginsenoside-Rh1 (19.77 mg/g), and 8MGL showed the highest content of ginsenoside-Re (20.43 mg/g). Total antioxidant activity (AEAC) was ranged from 5.56 at 4MGR to 20.67 mg AA eq/g at 8MGL.

경기도지역(京畿道地域)의 유우유방염(乳牛乳房炎)에 관(關)한 조사(調査) I 유방염(乳房炎)의 역학적(疫學的) 조사(調査)

손봉환,김효민,정흥환,김수장,Son, Bong Whan,Kim, Hyo Min,Jung, Heung Whan,Kim, Soo Chang 대한수의학회 1974 大韓獸醫學會誌 Vol.14 No.1

A total of 2,053 quarter milk samples of 518 dairy cows from 46 herds were examined for mastitis and results obtained were as follows: 1. A total of 428 quarters(20.8%) of 271 dairy cows(52.3%) from 41 herds(89%) were found to be infected with mastitis. 2. It was found that 71 quarters(3.5%) of 41 cows(7.9%) from 21 herds(45.6%) were clinical mastitis and 357 quarters(17.3%) of 230 cows(44.4%) from 20 herds (43.4%) were subclinical mastitis. 3. The main causative organisms of the mastitis were Staphylococcus aureus(26%), Streptococcus uberis(16%), and Streptococcus agalactiae(10%). 4. The majority of causative organisms were very resistant to colistin(85.9%), penicillin(67%), and streptomycin(39%). Most of the other drugs were highly effective as inhibitor for the most causative organisms in vitro. However sulfisoxazole was moderatively effective against them.

유질개선(乳質改善)을 통한 락농가(酪農家) 소득증대(所得增大): SCC와 유방염(乳房炎)을 중심으로

손봉환 ( Bong Whan Sohn ),최진영 ( Gin Young Choi ),배도권 ( Do Kown Bae ),정충일 ( Chung Ill Chong ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.3

The study for a effect of monitoring on bovine mastitis was conduced for improvement of raw milk from Jan. to Dec. in 1996. Sampling the milk of 367 cows(1,406 quarters) from 5 herds in Inchon and were carried out California mastitis test(CMT), somatic cell count(SCC), isolation of pathogens and antibiotic sensitivity tests. The results were summarized as follows, 1. The number of bovine mastitis was 177 cows(48.2%) and 371 quarters(26.4%); clinical mastitis: 25 cows(6.8%), 32 quarters(2.3%) and subclinicsl mastitis: 152 cows(41.4%), 339 quarters(24.1%). Incidence rate of mastitis by season were Summer 52.0%, Fall and Winter 48% and Spring 41%. Incidence rate of mastitis by quarters were Summer 30%, Fall 28%, Winter 25% and Spring 21%, respectively. 2. In the distribution of CMT degree by quarter, CMT positive(CMT±) of 1,406 quarters milk were 50.1%(704 quarters). The ratio of CMT positivity by quarter were left front quarter 55.8%, right front quarter 48.9%, right hind quarter 48.6% and left hind quarter 47%. The ratio of CMT positivity by season were Summer 54.1%, Fall 49.7%, Spring 48.5% and Winter 48%. 3. The highest mean SCC by season among 5 herds was “A” herd. Mean SCC (cell/ml) ofA herd were Summer 2,032,000cells/ml, Fall 1,109,000cells/ml, Winter 782,000cells/ml and Spring 577,000cells/ml. The lowest mean SCC by season among 5 herds was “E” herds. Mean SCC of E herd were Summer 1,064,000cells/ml, Spring 795,000cells/ml, Fall 429,000cells/ml and Winter 400,000cells/ml. Mean SCC of the other herds by season were little difference. 4. The milk samples of “A” herd were collected from 10 cows. In 3 seasons, mean 5CC of No. 2 and 3 cows were than 1,000,000cells/ml. In 1 season, mean SCC of No. 6, 7 and 8 cows were than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Summer 4 cows, Winter 3 cows, Spring and Fall 1 cow respectively. The milk samples of “B” herd were collected from 14 cows. In 3 seasons, mean SCC of No. 1 cow was more than 1,000,000cells/ml. In 2 seasons, mean SCC of No. 5,9 and 14 cows were more than 1,000,000cells/ml. In 1 season, No. 3,6 and 7 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Fall and Winter 4 cows respectively, Summer 3 cows and Spring 1 cow. The milk samples of “C” herd were collected from 18 cows. In 2 seasons, mean SCC of No. 16 cow was more than 1,000,000cells/ml. In 1 season, mean SCC of No. 1, 2, 6, 7, 13, 15 and 18 cows were more than 1,000,000cells/ml respectively. The more than mean SCC 1,000,000cells/ml of cows by season were distributed Summer 5 cows, Fall 3 cows, Spring 2 cows and Winter 1 cow. The milk sampes of “D” herd were collected 24 cows. In 3 season, mean SCC of No. 14 cow was more than 1,000,000cells/ml. In 2 seasons, mean SCC of No. 14 and 18 cows were more than 1,000,000cells/ml. In 1 season, mean SCC of No. 1, 2, 3, 8, 12, 17, 19, 20 and 21 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows were distributed Fall 15 cows, Spring and Winter 4 cows respectively and Summer 3 cows. The milk samples of “E” herd were collected from 27 cows. In 2 seasons, mean SCC of No. 6, 7 and 21 cows were more than 1,000,000cells/ml. In 1 season, mean SCC of No. 2, 4, 7, 11, 14, 16 and 23 cows were more than 1,000,000cells/ml. The more than mean SCC 1,000,000cells/ml of cows were distributed Spring and Fall 5 cows respectively, Summer and Winter 2 cows, respectively. 5. The rate of isolated pathogenic microorganisms from bovine mastitis were summarized as follows: Staphylococcus sp 168 strains(45.8%), Streptococcus sp 82 strains(22.3%), Gram(-) sp 45 strains(12.3%), Gram(+) sp 51 strains and the other sp 21 strains(5.7%). 6. The highest of antibiotic sensitivity test of each microorganism was summarized as follows: Staphyolcoccus sp-cephalosporin 76%, gentamicin 55%, Streptococcus sp-ampicillin 61%, cephalosporin 63%, Gram(-) sp-gentamicin 58%, Gram(+) sp-cephalosporin 63%, The other sp-cephalosporin 90%. Microorganisms showed the highest sensitivity(68%) to cephalospsorin.

주식시장 네트워크에서 클러스터링 기법을 이용한 포트폴리오 구성 방법

천봉환 ( Bong-hwan Chun ),김은경 ( Eun-kyung Kim ),정인준 ( In-jun Jung ),우균 ( Gyun Woo ) 한국정보처리학회 2012 한국정보처리학회 학술대회논문집 Vol.19 No.1

본 논문은 주식 투자 포트폴리오를 구성하기 위해 클러스터링 기법을 이용하는 방법을 제안한다. 클러스터링 기법은 패턴 공간 상의 특징 벡터로 표현된 패턴 데이터를 몇 개의 부분집합으로 나누는 작업을 의미한다. 본 연구에서는 주식시장 네트워크에 클러스터링 기법을 적용하여 안정성과 수익률이 높은 포트폴리오를 구성하는 방법을 제안한다. 그리고 추천 클러스터의 투자 적합여부를 데이터를 통해 확인한다. 2007년 주식 데이터를 대상으로 실험한 결과, 추천 클러스터의 수익률이 전체 수익률을 상회함을 확인할 수 있었다.

경기도지역(京畿道地域)의 유우유방염(乳牛乳房炎)에 관한 조사(調査) 4. 유우유방염(乳牛乳房炎)에서 분리(分離)한 Staphylococcus와 Streptococcus의 항균제(抗菌劑)에 대한 감수성시험(感受性試驗)

손봉환,김효민,한웅주,정흥환,김수장,Son, Bong Whan,Kim, Hyo Min,Han, Joo Woong,Jung, Heung Whan,Kim, Soo Chang 대한수의학회 1975 大韓獸醫學會誌 Vol.15 No.1

The susceptibility of 536 isolates of Staphylococcus and 313 isolates of Streptococcus to a number of chemotherapeutics were studied. These organisms were isolated from bovine mastitis during 1973 and 1974. In addition to this, the rate of multiple resistance of 425 isolates of Staphylococcus and 164 isolates of Streptococcus, isolated in 1974, to the chemotherapeutics was analysed. The results obtained in this work were summerized as follows: 1. Staphylococcus and Streptococcus isolated in 1974 showed a higher resistance, with 3 exceptions of chemotherapeutics, than the isolates of 1973. 2. Staphylococcus isolated in 1973 and 1974 showed a higher susceptibility than Streptococcus. 3. The strains of Staphylococcus resistant to colistin were 39 strains (9.2%), to colistin and sulfisoxazole 33 (7.8%), to streptomycin, kanamycin, colistin and sulfisoxazole 20 (4.7%), and to penicillin, colistin and sulfisoxazole 18 (4.2%). 4. The strains of Streptococcus resistant to colistin were 17 strains (10.4%), to streptomycin, kanamycin, colistin and sulfisoxazole 13 (7.9%), to colistin and sulfisoxazole 11 (6.7%) and to penicillin, colistin and sulfisoxazole 11 (6.7%).