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폐암 진단에 적용 가능한 측면 유동 면역 형광 분석법 개발
뮬야수피안토,임정민,이혜진,Supianto, Mulya,Lim, Jungmin,Lee, Hye Jin 한국공업화학회 2022 공업화학 Vol.33 No.2
A lateral flow immunoassay (LFIA) method using carbon nanodot@silica as a signaling material was developed for analyzing the concentration of retinol-binding protein 4 (RBP4), one of the lung cancer biomarkers. Instead of antibodies mainly used as bioreceptors in nitrocellulose membranes in LFIA for protein detection, aptamers that are more economical, easy to store for a long time, and have strong affinities toward specific target proteins were used. A 5' terminal of biotin-modified aptamer specific to RBP4 was first reacted with neutravidin followed by spraying the mixture on the membrane in order to immobilize the aptamer in a porous membrane by the strong binding affinity between biotin and neutravidin. Carbon nanodot@silica nanoparticles with blue fluorescent signal covalently conjugated to the RBP4 antibody, and RBP4 were injected in a lateral flow manner on to the surface bound aptamer to form a sandwich complex. Surfactant concentrations, ionic strength, and additional blocking reagents were added to the running buffer solution to optimize the fluorescent signal off from the sandwich complex which was correlated to the concentration of RBP4. A 10 mM Tris (pH 7.4) running buffer containing 150 mM NaCl and 0.05% Tween-20 with 0.6 M ethanolamine as a blocking agent showed the optimum assay condition for carbon nanodot@silica-based LFIA. The results indicate that an aptamer, more economical and easier to store for a long time can be used as an alternative immobilizing probe for antibody in a LFIA device which can be used as a point-of-care diagnosis kit for lung cancer diseases.
멀티페이즈 컨버터 기반 3㎾급 연료전지용 계통연계 PCS 개발
한동화(Donghwa Han),최규하(Gyuha Choe),채영민(Youngmin Chae),조준석(Junseok Cho),임정민(Jungmin Lim) 전력전자학회 2010 전력전자학술대회 논문집 Vol.2010 No.7
본 논문은 연료전지를 이용한 가정용 발전시스템의 계통 연계를 위한 3㎾급 전력변환 시스템의 개발에 관한 것이다. 저전압 대전류 출력특성을 갖는 연료전지의 전류리플 최소화를 위해 멀티페이즈 컨버터를 적용한 DC/DC 승압 컨버터를 제안하고자 한다. 특히 고밀도 전력변환 시스템의 스위칭 손실 극소화를 위해 고효율 ZVS Soft-Switching 동작을 구현하였다. 또한 본 연구에서는 높은 승압비로 변환된 전압을 통해 계통운전 동작을 수행하기 위한 DC/AC 단상 인버터와 고효율 무결점 기능을 갖는 디지털 방식의 DSP를 적용한 제어기 개발도 병행하여 수행되었다. 제안하는 3㎾급 멀티페이즈 컨버터 및 계통연계형 인버터의 Proto-Type을 제작하고 실험을 통해 본 방식의 타당성을 검증하였다.
REMOTE MONITORING OF WIND-PHOTOVOLTAIC HYBRID GENERATION SYSTEM USING MOBILE PHONE AND INTERNET
허정초(Xu Zhenchao),문채주(Moon Chaejoo),장영학(Chang Younghak),임정민(Lim Jungmin),김태곤(Kim Taegon) 대한전기학회 2007 대한전기학회 학술대회 논문집 Vol.2007 No.11
In this paper, a remote monitoring system of wind-photovoltaic hybrid generation system using mobile phone and internet has been developed. Many kinds of data can be acquired, analyzed and saved automatically by this system. The hybrid system is composed of l[㎾] PV with DC/DC converter, battery banks and 5[㎾] wind power system with power inductor and AC/DC converter. In addition, wind monitoring sensors, voltage and current meters, current transformers and potential transformers are used as accessory instruments. All of these signals are fed into DAQ <Data Acquisition> board after converting the data which have been processed by many types of converters, dividing circuits and signal conditioning circuits. These data can not only be displayed on a computer, transmitted using the server program to remote computer and saved on a computer as a file day by day but also be sent as a CDMA message. The monitored-data can be downloaded, analyzed and saved from server program in real-time via mobile phone or internet at a remote place. All of the programs were designed with Lab VIEW software.