각막신생혈관에 대한 버테포르핀을 이용한 광역학치료의 동물실험

나현주,윤경철,임욱빈,안규윤,서만성,Hyeon-Ju Nah,M,N,Kyung-Chul Yoon,Wook-Bin Im,Kyu-Youn Ahn,Man-Seong Seo 대한안과학회 2005 대한안과학회지 Vol.46 No.4

Purpose: To determine the efficacy of photodynamic therapy (PDT) with verteporfin (Visudyne?, Norvatis Ophthalmics AG, Hettingen, Switzerland), a benzoporphyrin derivative, in the treatment of corneal neo- vascularization (CN) in a rabbit eye model. Methods: CN was induced by placing instrastromal sutures in the cornea. Two weeks after suturing, verteporfin was administrated intravenously and 1 hour later, the right eye (treated group) was exposed to a laser with a 689 nm wavelength, and the left eye was used as the control. The changes in CN were analyzed using biomicroscopy and optical microscopy in twelve rabbits. Results: The mean percentages of the neovascular area in the control and treated groups were 96.4±1.9% and 90.3±3.5% (P=0.009) at three days after the PDT, 88.6±4.6% and 71.6±6.2% (P<0.001) at one week, and 76.8±4.4%와 43.6±15.1% (P<0.001) at two weeks, respectively. Optical microscopy showed significant differences between the control and treated group in terms of the area and number of CN (P<0.05). Conclusions: PDT with verteporfin is a safe and effective procedure for regressing CN. However, a further study will be necessary.

곡물류의 형질전환에 관한 연구.II. Electroporation에 의해 벼 원형질체로 도입된 유전자의 발현

황백,황성진,임욱빈,임형탁,강영희,Hwang, Baik,Hwang, Sung-Jin,Im, Wook-Bin,Im, Hyong-Tak,Kang, Young-Hee 한국생물공학회 1990 KSBB Journal Vol.5 No.4

Protoplasts isolated from embryogenic cell suspensions were electroporated in buffered solutions containing plasmid DNA of pBI121. Transient GUS (beta-glucuronidase) activity measurement and selection for kanamycin resistent showed that expression of foreign genes and stable loransformation were achieved. GUS transient gene expression was increased by increasing DNA concentration of pBI121 plasmid and affected by the level of the applied voltage. An optimal level of GUS activity was obtained after electroporation with a pulse of 200 voltage/1180 uF. Protoplast viability was up to the 60% at the optimal voltage. Cell colonies resistent to 200$\mu\textrm{g}$/ml kanamycin were selected in agar medium and identified by histochemical GUS assay.

빠른 교대근무가 요중 Na⁺, K⁺, Cl⁻의 배설에 미치는 영향

민순(Min, Soon),문대수(Moon, Dae Soo),임욱빈(Im, Wook-Bin) 한국간호과학회 1998 Journal of Korean Academy of Nursing Vol.28 No.4

곡물류의 형질전환에 관한 연구 Ⅱ. Electroporation에 의해 벼 원형질체로 도입된 유전자의 발현

황백(Baik Hwang),황성진(Sung Jin Hwang),임욱빈(Wook Bin Im),임형탁(Hyong Tak Im),강영희(Young Hee Kang) 한국생물공학회 1990 KSBB Journal Vol.5 No.4

교대근무 방향이 요중 Free cortisol, 17-Hydroxycorticosteroid의 Circadian Rhythm에 미치는 영향

민순(Min Soon),박영진(Park Young Jin),김미승(Kim Mi Seung),이현주(Lee Hyun Joo),임욱빈(Im Wook Bin) 한국성인간호학회 1999 성인간호학회지 Vol.11 No.2

치매간호중재 프로그램이 경증 치매노인의 Na, K, Ca, Cl, P, Cholesterol의 혈중농도 및 17-KS, 17-OHCS의 뇨배설량에 미치는 영향

나현주,조명숙,임욱빈 대한기초간호자연과학회 2002 Journal of korean biological nursing science Vol.4 No.2

This study was conducted for the evaluation of the effect of Dementia prevention nursing program on Urinary 17-KS, 17-OHCS, and Blood Na, Cl, K, Ca, P, cholesterol. The sample for the present study was composed of 16 demented elderly in D care center for the eldery in K city. The Dementia prevention nursing program consisted of concept memory training, music therapy, and art therapy. The program was performed twice a week and about ninety minutes was consumed for one session. The program had been administered for 8 weeks. We checked Urinary 17-KS, 17-OHCS, and Blood Na, Cl, K, Ca, P, cholesterol befor and after the program. Statistical analysis was performed by using descriptive statistics and Wlicoxon signed rank test of SAS system for window 6.12. The result were follows : 1. Urinary 17-KS 17-KS score increased significantly after intervention(p=0.005). 2. Urinary 17-OHCS 17-KS score increased significantly after intervention(p=0.00l). 3. Blood Na, Cl, K, Ca, P, cholesterol Na(p=0.0002), Cl(p=0.0001) K(p=0.0001), Ca(p=0.0028) decreased significantly after intervention. The results show that Dementia prevention nursing program increases Urinary 17-KS, the Dementia 17-OHCS, and decreases Blood Na, Cl, K, Ca, P, cholesterol. In conclusion, prevention nursing program can be used for the effective measure to decrease stress of the Demented elderly.

야간 근무시의 혈장 멜라토닌의 농도변화 : 시계 방향과 반시계 방향의 교대근무 비교 Comparison of the Clockwise and Counterclockwise Rotational Shift

민순,김미승,임욱빈 대한기초간호자연과학회 2000 Journal of korean biological nursing science Vol.2 No.2

To determine the optimal rotational shift system, the effect of the direction of the rotational work shifting on the nocturnal rhythm of plasma melatonin were investigated in nursing students. Two groups of nine volunteers participated as experimental subjects, and two nursing students participated as a control group. The directions of the rotational work shift were as follows : CW(clockwise)-shift were rotated in the direction of day shift(3 days), evening shift(3 days), off duty(1 day) and night shift(5 days), and CCW(counterclockwise)-shift were done in the reverse direction. Plasma melatonin concentrations was measured by radioimmunoassay. The results were as follows : The plasma melatonin levels were kept low at night and in the following morning in the CW night shift workers, whereas the level started to increase at 05 : 00 hr in two workers of four CCW shift workers. These result suggests that the shift rotation in the CW direction is more acceptable in terms of the adaptation of hormonal rhythms. These results indicate that the CCW rotation of shift work is somewhat better than CCW rotation for the adaptation to shift work on hormonal aspects in nurses.

2일 간격의 교대근무가 요중 17-OHCS와 Free Cortisol 농도에 미치는 효과

백윤웅,임욱빈,민순 성인간호학회 1997 성인간호학회지 Vol.9 No.3

In this study, 20 students in C university were selected by control group and 14 shift work nurses working in C university hospital by test group. It took part in case of day shift(8AM-4PM), evening shift(4PM-12MN), and night shift (12MN-8AM) to know a urinary 17-OHCS and free cortisol changes which works in a rapidly rotating shift work system. The working team were an rest period of a day and an observation of a week. It obtained an urine specimen before and after work shift in 2nd day. Test group and control group of shift work compared to change of levels of urinary 17-OHCS and free cortisol of urine collected from nurses of day shift, evening shift, and night shift. The data was analyzed by t-test, paired t-test. The results are as follows. 1. Compare 8AM with 4PM in day shift. Control group increased 4.1mg at 8AM, 4.2mg at 4PM in a case of 17-OHCS(p=.84) and also test group increased a little 3.5mg at start time of work, 3.6mg at stop time(p=.97). In a case of free cortisol control group decreased 3.8㎍ at 8AM, 2.4㎍ at 4PM(p=.12) and test group decresed 8.3㎍ at start time of work, 3.2㎍ at stop time(p=.22). 2. Compare 4PM with 12MN in evening shift. Control group decreased 4.2mg at 4PM, 2.9mg at 12MN in a case of 17-OHCS(p=.54), but test group increased 1.7mg at start time of work, 3.4mg at stop time(p=.07). In a case of free cortisol control group decreased 2.4㎍ at 4PM, 1.9㎍ at 12MN(p=.23) and also test group decresed a little 2.6㎍ at start time of work, 1㎍ to stop time(p=.43). 3. Compare 12MN with 8AM in night shift. Control group increased 3.9mg at 12MN, 4.1mg at 8AM in a case of 17-OHCS(p=.79) and also test group increased 6.3mg at start time of work, 8.4mg at stop time(p=.16). In a case of free cortisol control group increased 1.9㎍ at 12MN, 3.8㎍ at 8AM(p=.08) and test group increased 4.4㎍ at start time of work, 11.6㎍ at stop time(p=.04). As a result of this study reveals that health level of nurses is decreased in a rotating shift work(2days interval). As mentioned above, it was confirmed that the rotating shift work had influence on the health of the nurses. For it, I present the objective base data to mesure the level healthy in order to extent the understanding of the physical aspects of the nurses. Moreover, it is considered that one can make use of it as an objective base with a view to the rational management for the nursing administration.

Seasonal Variation in the Na^+, K^+-ATPase Activity in Frog (Rana dybowskii) Brain

Kim, Mi-Seung,Im, Wook-Bin 한국동물학회 1995 동물학회지 Vol.38 No.4

Mechanisms of Tributyltin-induced Leyding Cell Apoptosis

Lee, Kyung Jin,Kim, Deok Song,Ra, Myung Suk,Wui, Seong Uk,Im, Wook Bin,Choi, Hueng Sik,Lee, Jong Bin 한국환경독성학회 2003 환경독성보건학회지 Vol.18 No.2

본 연구는 환경 호르몬으로 분류된 67종 중의 하나인, 선저 도료나 어망, 어구 및 방오페인트 재료로 사용되어지고 유기주석화합물(tributyltin)을 사용하여 설치류의 웅성생식세포에서 세포자연사를 일으키는 작용기작을 조사하였다. 먼저 흰쥐의 레이딕 세포주인 R2C에 유기주석화학물을 농도별 (1~500nM)로 처리한 후 DNA fragment현상을 전기영동법을 통하여 조사하였다. 그 결과 유기주석화합물을 처리한 군들에서 대조군에 비하여 세포자연사현상이 농도 의존적으로 증가하였다. 유기주석화합물이 세포 내 칼슘이온(Ca^(2+)) 및 유해 산소종(reactive oxygen species)에 미치는 영향을 조사해본 결과 유기주석화합물 처리시 세포 내 칼슘이온 및 유해 산소종이 시간에 의존적으로 크게 증가하였다. 또한 칼슘 킬레이터인 BAPTA를 전 처리한 경우 유기주석화합물에 의해 유도된 칼슘이온 및 유해 산소종이 대조군에 비해 유의성 있게 감소하였다. 이러한 세포자연사 과정이 미토콘드리아의 cytochrome c 방출에 의한 과정인지를 확인하기 위해 세포질 내 cytochrome c 양을 western blot법을 사용하여 확인해 본 결과 유기주석화합물 처리 시간 및 농도에 따라 증가하며, 이 또한 BAPTA를 전처리 한 경우 대조군에 비하여 유의성 있게 감소하였다. 또한 유기주석화합물이 세포자연사 유발 시 caspase-3 효소 활성과의 관계를 확인하기 위해 ELISA법을 사용하여 확인해 본 결과 유기주석화합물 처리 농도에 의존적으로 증가하였으며, caspase-3 효소 억제자로 잘 알려진 Z-DEVD FMK을 전 처리한 경우 유기주석화합물을 처리한 군에 비해 세포자연사율이 크게 감소하였다. 이러한 결과들을 종합해 볼 때 유기주석화합물은 세포 내 칼슘이온의 증가를 일으키고, 그로 인하여 세포질 내 유해산소종 및 cytochrome c의 양이 증가함으로써 세포자연사 다음 단계인 caspase 효소 활성의 증가를 통하여 흰쥐의 레이딕 세포주인 R2C의 세포자연사를 일으킬 것이라 추론할 수 있다. Tributyltin(TBT) used world-wide in antifouling paints for ships is a widespread environmental pollutant and cause reproductive organs atrophy in rodents. At low doses, antiproliferative modes of action have been shown to be involved, whereas at higher doses apoptosis seems to be the mechanism of toxicity in reproductive organs by TBT. In this study, we investigated that the mechanisms underlying DNA fragmentation induced by TBT in the rat leydig cell line, R2C cells by fluorescence detector. TBT significantly induced intracellular Ca^2+ level in a time-de-pendent manner. The rise in intracellular Ca^2+ level was followed by a time=dependent generation of reactive oxygen species (ROS) at the cytosol level. Simultaneously, TBT induced the release of cytochrome c from the mitochondrial membrane into the cytosol. Furthermore, ROS production and the release of cytochrome c were reduced by BAPTA, an intracellular Ca^2+ chelator, indicating the important role of Ca^2+ in R2C during these early intracellular events. In addition, Z-DEVD FMK, a caspase-3 inhibitor, decreased apoptosis by TBT. Taken together, the present results indicated that the apoptotic pathway by TBT might start with an increase in intracellular Ca^2+ level, continues with release of ROS and cytochrome c from mitochondria, activation of caspases, and finally results in DNA fragmentation.