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사료 첨가용 생균제를 위한 Probiotics 유산간균의 분리 및 동정
이승배,최석호,Lee Seung-Bae,Choi Suk-Ho 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.1
한우와 홀스타인의 분변으로부터 MRS 배지와 LAPT 배지를 이용하여 무작위 선발법으로 54균주의 유산균을 1차로 분리하였다. 1차로 분리된 54균주에 대해 내담즙성이 우수한 10균주를 분리한 다음 내산성을 조사한 결과 인공위액 pH 2.5에서 LS1, LS15 및 LL6 균주가 각각 66.5%, 82.6% 및 80.7%의 생존율을 나타내었다. Sal. typhimurium, Sta. aureus 및 Cl. perfringens의 병원균에 대해 가장 큰 항균력을 보인 균주는 LL6와 LL7이었다. API CHL kit로 동정한 결과 LS1, LS2 및 LMI 균주는 모두 L. fermentum, LL6와 LL7 균주는 L. acidophilus, LS3 균주는 L. plantarum으로 각각 동정되었고, 나머지 4균주는 Lactobacillus spp. 로 동정되어 분리된 10균주 모두 안전성 있는 유산간균임을 확인하였다. 10종류의 항생제에 대한 내성을 조사한 결과 ampicillin, amoxicillin과 erythromycin에 대해서는 감수성이 있으나 colistin과 ciprofloxacin에 대해 모두 내성을 나타내었다. LB1, LL6 및 LL7 균주는 gentamicin과 neomycin에 대해 내성을 보여 주었다. 분리 동정된 균주 중에 내산성, 내담즙성 및 병원성 균에 대한 특성을 기준으로 판단할 경우 probiotic 유산균으로 사용 가능성이 높은 것은 LL6인 L. acidophilus로 나타났다. Fifty four acid-resistant and bile-resistant isolates of lactic acid bacteria were initially isolated from the faces of Korea native cattle and Holstein using MRS agar and LAPT agar, and ten strains with superior activity against bile salt were finally selected LS1, LS15, and LL6 isolates showed survival of 66.5%, 82.6% and 80.7% against the simulated stomach liquid(pH 2.5), respectively. LL6 and LL7 isolates had the highest inhibitory activities against the pathogenic bacteria such as Salmonella typhimurium, Staphylococcus aureus, and Clostridium perfringens. By using API 50 CHL kit LS1, LS2 and LM1 isolates were identified as a L. fermentum. LL6 and LL7 isolates as a L. acidophilus, and LS3 isolate as a L. plantarum. The other four isolates belong to genus Lactobacillus. All the isolates tested were sensitive to some antibiotics such as ampicillin, amoxicillin and erythromycin, but resistant to colistin and ciprofloxacin. LB1, LL6 and LL7 isolates were resistant to gentamicin and neomycin. Especially, the LL6 isolate showed the highest resistance to both of the simulated stomach liquid and bile salt, in addition to the highest inhibitory activities against Sal. typhimurium, Staph. aureus and Cl. perfringens.
Birnessite와 Hydroxyapatite에 의한 납과 카드뮴 오염퇴적토의 독성저감 평가
이승배,정재웅,김영진,남경필,Lee, Seung-Bae,Jung, Jae-Woong,Kim, Young-Jin,Nam, Kyoung-Phile 한국지하수토양환경학회 2012 지하수토양환경 Vol.17 No.4
The success of stabilization treatment in heavy metal contaminated sediment depends on the heavy metal bioavailability reduction through the sequestration of the heavy metals. This study was performed to assess the changes in the bioavailability of Pb or Cd in the Pb or Cd contaminated sediments by using birnessite and hydroxyapatite as stabilizing agents. The toxicity tests were carried out using a microorganism (Vibrio fischeri), an amphipod (Hyalella azteca) and an earthworm (Eisenia foetida). With Vibrio fischeri, the toxicities of both Pb and Cd were reduced by more than ten times in the presence of birnessite and hydroxyapatite compared to that of in the absence of birnessite and hydroxyapatite. The concentrations of Pb and Cd in the contaminated sediments were lethal to Hyalella azteca, however, in the presence of birnessite and hydroxyapatite more than 90%, on average, of Hyalella azteca survived. With Eisenia foetida, the bioaccumulated concentrations of both Pb and Cd were reduced by more than 75%, on average, lower with the addition of birnessite and hydroxyapatite to the contaminated sediments. These results show that the addition of birnessite and hydroxyapatite can reduce the bioavailability of Pb and Cd in contaminated sediments. In addition, the in situ and ex situ performance of birnessite and hydroxyapatite as stabilizing agents can be verified using the toxicity tests with Hyalella azteca and Eisenia foetida, respectively.
축대칭 물체의 경계층 유동소음에 대한 실험적 연구 ( I ) - 축대칭 물체 전두부 및 실린더 벽면 섭동압력 -
이승배,김휘중,권오섭,이상권,Lee, Seung-Bae,Kim, Hooi-Joong,Kwon, O-Sup,Lee, Sang-Kwon 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.7
The axisymmetric bodies considered in this study have hemispherical and ellipsoidal noses. The near-field pressure fluctuations over each nose model at $Re_D=2.43{\times}10^5$ were investigated in the laminar separation region and developing turbulent boundary layers using a 1/8' pin-holed microphone sensor. The wall pressure fluctuations were also measured in an axisymmetric boundary layer on a cylinder parallel to mean flow at a momentum thickness Reynolds number of 850 and a boundary layer thickness to cylinder radius ratio of 1.88.
축대칭 물체의 경계층 유동소음에 대한 실험적 연구(II) - 전두부 천이제어 및 방사소음 -
이승배,김휘중,권오섭,이상권,Lee, Seung-Bae,Kim, Hooi-Joong,Kwon, O-Sup,Lee, Sang-Kwon 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.10
The oscillatory excitation with a Strouhal number of 2.65 ncar the stagnation zone of hemispherical nose model was employed to control the laminar separation bubble and the transition to turbulence. The effects of oscillatory excitation upon the separation bubble and the transition were addressed in terms of kurtosis/skewness and time-frequency analyses. The measured noise spectrum of radiated sound from the turbulent boundary layer on the axi-symmetric infinite cylinder is compared with that by Sevik's wave-number white approximations. The noise sources in TBL on axi-symmetric cylinder and the caling of their far-field sound are also discussed.
Duplex PCR을 이용한 유제품 안에 있는 산양유와 우유의 신속한 동정에 대한 연구
이승배,최석호,Lee, Seung-Bae,Choi, Suk-Ho 한국축산식품학회 2009 한국축산식품학회지 Vol.29 No.5
A duplex PCR technique was applied for specific identification of cow and goat milk in milk products by using primers targeting the mitochondrial 12S rRNA gene. Duplex PCR using primers specific for cow and goat generated specific fragments of 223bp and 326bp from cow and goat milk DNA, respectively. Duplex PCR was applied to 15 milk products purchased from the market to verify label statements. The labeling statements of four market milk products, three yoghurt products, and one whole milk powder product were confirmed in the duplex PCR. The labeling statements of five of seven infant milk powder products were also confirmed by duplex PCR but the other two products were shown to be contaminated with either cow or goat milk. The proposed duplex PCR provides a rapid and sensitive approach to detection of as little as 0.1% cow milk in goat milk and one-step detection of cow or goat milk in milk products.
이승배(Seung Bae Lee) 한국스포츠교육학회 2012 한국스포츠교육학회지 Vol.19 No.2
본 연구는 초등학교 축구형 게임수업의 이해중심적 접근을 위한 수정모형을 착안하고, 수업실천을 통해 착안된 모형의 효과를 검토함으로써 현장지향적인 수정모형을 개발하는데 목적이 있다. 이에 2011년 1월부터 10월까지 6학년을 대상으로 Kemmis & McTaggart(2000)의 ``나선형의 자기반성적 사이클``을 변형한 실행연구를 수행하였다. 자료의 수집 및 분석은 Spardley(1979, 1980)가 제안한 ``발전식 연구 단계``에 의해 영역분석, 분류분석, 성분분석을 순차적으로 진행하였고, 연구자 반성일지, 수업관찰 포토에세이, 심층 면담, 집단 면담 및 토론, 학생기록물, 전문가협의회 및 문헌 자료를 수집하였다. 연구 결과, ``반성적 순환모형``은 게임규칙의 이해 및 적용 능력 증진, 전술지식의 향상, 스포츠맨십의 내면화와 팀워크 형성 등 게임의 성격 및 구조를 이해하는데 매우 효과적인 모형으로 확인되었다. 그러나 본 수정모형은 기초기능이나 골결정력 향상에는 뚜렷한 가시적인 성과를 보이지는 않았으며, 성공적인 모형 적용을 위해서는 효율적인 수업 운영의 구조가 중요한 것으로 드러났다. 따라서 학교현장의 게임지도 시 게임의 성격 및 구조 이해를 위한 이해중심적인 접근이요구되며, 교사는 자신의 수업에 맞는 수정모형의 개발 및 적용을 위한 적극적인 시도와 노력이 요구된다. The purpose of this study was to develop the field-oriented revised model, which approaches to teaching games for understanding(TGfU) by examining the effects of learning through practicing physical education(PE) classes in elementary school. To achieve this purpose, researcher applied action research, modified Kemmis and McTaggart`s a spiral of self-reflective cycles. Participants were 6th grade elementary school students. Date were collected and analyzed by the developmental research sequence(D.R.S.), such as domain analysis, taxonomic analysis, and componential analysis, which was proposed by Spardley. The collected data were researcher`s reflective journal, photo essay, in-depth interview data, focus group interview and discussion data, student`s portfolio, auditing data, and documents. The major findings are as follows: ``The model of reflective circulation`` is identified as an effective model for understanding the properties and structure of games, such as enhancement of abilites to understand and applicate the rules of games, improvement of tactical knowledge, and internalization of sportsmanship and formation of teamwork. But it is not clearly show that this revised model enhances elementary school students` basic technique and scoring by shooting in PE classes. In addition, in order to apply this model, it`s important for teacher to build up the efficient structure of instructional management. In conclusion, as teachers conduct the PE classes for understanding the properties and structure of games, it is necessary for them to approach to teaching games for understanding and to try and make an effort for developing and applying their suitable instructional model.