인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

학교시설 무상공급 재정성과 및 제도개선 방안 연구

이상민,Lee, Sang-Min 한국교육녹색환경연구원 2015 교육·녹색환경연구 Vol.14 No.3

본 연구는 학교시설 무상공급제도에 따른 교육재정성과를 분석하기 위해 무상공급 현황 및 재정분석, 문제점 분석을 실시하였다. 이를 위해 수도권 학교시설 무상공급 관련 사업 현황을 조사하였으며, 제도 시행성과 및 문제점에 대한 설문조사를 실시하였다. 학교시설 무상공급은 교육재정에 대한 성과에도 불구하고 법제도 미비에 따른 협약 및 공사품질확보, 적정시기 개교 등에 있어서 많은 문제점을 드러내고 있으며 이를 개선하기 위한 법제도의 보완이 필요한 것으로 분석되었다. 2009 As the School District is amended as a metropolitan area public development operators obliged to free supply of school facilities contribute to the streamlining of the provincial education funding, and although institutions shortly became implemented in earnest, the effect has been, despite a number of problems occur. In this study, analysis of these school facilities free of charge supply system on the status and problems through surveys, and suggests directions for future drafting and improving legislation.

고온관 누설에 의한 가압열충격 사고시 원자로 용기의 건전성 평가를 위한 결정론적 파괴역학 해석

이상민,최재붕,김영진,박윤원,정명조,Lee, Sang-Min,Choi, Jae-Boong,Kim, Young-Jin,Park, Youn-Won,Jhung, Myung-Jo 대한기계학회 2002 大韓機械學會論文集A Vol.26 No.11

In a nuclear power plant, reactor pressure vessel (RPV) is the primary pressure boundary component that must be protected against failure. The neutron irradiation on RPV in the beltline region, however, tends to cause localized damage accumulation, leading to crack initiation and propagation which raises RPV integrity issues. The objective of this paper is to estimate the integrity of RPV under hot leg leaking accident by applying the finite element analysis. In this paper, a parametric study was performed for various crack configurations based on 3-dimensional finite element models. The crack configuration, the crack orientation, the crack aspect ratio and the clad thickness were considered in the parametric study. The effect of these parameters on the maximum allowable nil-ductility transition reference temperature ($(RT_{NDT})$) was investigated on the basis of finite element analyses.

경두개 자기 자극이 인지 기능에 미치는 영향

이상민,채정호,Lee, Sang Min,Chae, Jeong-Ho 대한생물정신의학회 2016 생물정신의학 Vol.23 No.3

Transcranial magnetic stimulation (TMS) is a safe, noninvasive and useful technique for exploring brain function. Especially, for the study of cognition, the technique can modulate a cognitive performance if the targeted area is engaged, because TMS has an effect on cortical network. The effect of TMS can vary depending on the frequency, intensity, and timing of stimulation. In this paper, we review the studies with TMS targeting various regions for evaluation of cognitive function. Cognitive functions, such as attention, working memory, semantic decision, discrimination and social cognition can be improved or deteriorated according to TMS stimulation protocols. Furthermore, potential therapeutic applications of TMS, including therapy in a variety of illness and research into cortical localization, are discussed.

정적 O/D자료와 실시간 동적 교통정보의 생성과 관리

이상민,신희철,유재광,Lee, Sang-Min,Sin, Hui-Cheol,Yu, Jae-Gwang 대한교통학회 2005 교통 기술과 정책 Vol.2 No.1

주성분 분석 기법을 이용한 심음 인식

이상민,홍승홍,Lee, Sang-Min,Hong, Seung-Hong 대한전자공학회 2001 電子工學會論文誌-SC (System and control) Vol.38 No.5

Recently many researches concerning heart sound analysis are being processed with development of digital signal processing and electronic components. But there are few researches about recognition of heart sound, especially full cardiac cycled heart sound, In this paper, a new recognition methods about. full cardiac cycled heart sound was proposed. For the first, the database was built by principal components analysis on training heart sound set. This database is used to recognize new input of heart sound, Ilear sounds were classified into seven classes such as normal(NO) class, pre-systolic murmurr(PS) class, early systolic murmur(ES) class, late systolic murmurr(LS) class, early diastolic murmur(EI) class, late diastolic murmur(LD) class and continuous murmuru(CM) class. As a result, we could verify that our new method has better efficiencies for the recognition the characteristics of heart sound than any precedent research. The maximum recognition rates of the new method are 71% for NO, 80% for PS and ES, 78% for LS, 87% for ED, 60% for LD and 20% for CM. Although the present results aren't practically sufficient to use our new method in recognizing heart sound, the importance of this paper is for recognition of heart sound within full cardiac cycle. We can get a better result by building a more efficient database. 최근에 디지털 선호처리와 전자부품의 발달로 심음 분식에 관한 많은 연구가 진행되고 있다. 그러나 심음 인식, 특히 심음 한주기 전체에 대한 인식연구는 거의 없다. 본 논문에서 심음 전체 한주기에 대한 새로운 인식 방법을 제안하였다. 먼저 주성분 분석을 이용하여 훈련 셋트로 데이터베이스를 구축한다. 데이터베이스는 새로운 심음입력을 인식하는데 이용된다. 심음은 정상심음, 수축전 심잡음, 수축초기 심잡음, 수축 말기 심잡음, 이완 초기 심잡음, 이완 말기 심잡음, 연속적 심잡음으로 분류된다. 실험결과 새로운 인식 방법은 심음의 특징을 인식하는데 효과적이었다. 최대 인식률은 NO의 경우 71%, PS와 ES의 경우 80%, LS의 경우 78%, ED의 경우 87%, LD의 경우 60%, CM의 경우 20% 이었다. 현재의 결과가 실제적으로 심음을 인식하기에는 충분하지 못하였지만 선음 전체 주기를 대상으로 한 연구라는데 의의가 있으며 더 효과적인 데이터베이스를 구축함으로써 인식률을 개선할 수 있다.

사료의 $\alpha-cellulose$ 함량이 조피볼락 Sebastes schlegeli의 성장, 사료 효율 및 체성분에 미치는 영향

이상민,이종윤,LEE Sang-Min,LEE Jong Yun 한국양식학회 1994 韓國養殖學會誌 Vol.7 No.2

사료의 $\alpha-cellulose$ 함량이 조피볼락의 성장, 사료 효율, 영양소 이용 효율 및 체조성에 미치는 영향을 조사하기 위해 209 전후의 조피볼락 치어를 대상으로 사료의 $\alpha-cellulose$ 함량이 $0\~20\%$가 되도록 총 5종의 실험 사료를 제조하여 10주간 사육 실험한 결과는 다음과 같다. $\alpha-cellulose$ 함량 $10\%$ 첨가구까지는 대조구와 유의차없이 성장 및 사료 효율이 양호하였고, $\alpha-cellulose$ $15\%$이상 첨가 실험구들은 유의하게 낮은 값을 보였다(P<0.05). 일간 먹이 섭취량은 $\alpha-cellulose$ $15\%$ 이상 첨가구에서 유의하게 높은 결과를 보였다(P<0.05). 영양소 축적효율 중 단백질 및 에너지 축적 효율은 $\alpha-cellulose$ $10\%$ 첨가구까지 대조구와 유의차 없이 좋은 결과를 보인 반면, 지질 축적 효율은 a-cellulose 함량이 증가함에 따라 계속 감소하였다 (P< 0.05). 전어체의 단백질 함량은 실험 구간별로 차이가 없었지만, 지질 함량은$\alpha-cellulose$ $10\%$ 첨가구에서 유의하게 높은 값을 보였다(P<0.05). Experimental diets containing 5 different levels of a-cellulose $(0\%,\;5\%,\;10\%,\;15\%\;or\;20\%)$ were fed to juvenile Korean rockfish (20.5g of initial mean weight) for 10 weeks to study their ability to utilize dietary $\alpha-cellulose$. Weight gain, feed efficiency, and protein and energy retention efficiency in the fish fed diets containing $0\%,\;5\%,\;or\;10\%$ of $\alpha-cellulose$ were significantly higher than those in the fish fed diets containing $15\%\;or\;20\%$ of $\alpha-cellulose$ (P<0.05). Whole body lipid content of the fish fed $10\%$ $\alpha-cellulose$ diet were significantly higher than that of fish fed other diets (P<0.05). The results suggest that the optimum dietary fiber levels for Korean rockfish would be less than $10\%$.

저항 용접을 이용한 금속 샌드위치 판재 접합에 관한 연구 : Part 2 - 공정해석

이상민,김진범,나석주,Lee Sang-Min,Kim Jin-Beom,Na Suck-Joo 대한용접접합학회 2005 대한용접·접합학회지 Vol.23 No.6

In part 1. optimal process parameters such as thickness of stopper and welding time are achieved to produce high strength ISB(Inner Structured and Bonded) panels. Developed process is different from the usual resistance welding process in the number of points welded at a time. In part 2, Numerical modeling for this new process is proposed and the variation of contact area with respect to the gap of electrodes is studied through FE analyses, Besides, it is tried to figure out the welding nugget formation and proper distance between welding points. FE analytic results show that inner structures are melted more than skin plate, and current distribution between points to be welded can be controlled by distance welding points. Comparison of some FE analytic results with corresponding experimental results could confirm the validity of the proposed numerical modeling.

조피볼락 육석용 배합사료와 시판사료의 사육효과 비교

이상민,전임기,Lee Sang-Min,Jeon Im-Gi 한국양식학회 1996 韓國養殖學會誌 Vol.9 No.4

실용적인 사료의 효과를 검토하기 위해 단백질함량이 $41\~45\%$인 4종류의 실험사료를 dry pellet 형태로 제조하였다. 단백질원으로 어분을 $58\%$ 첨가한 사료를 대조구(사료 1)로 하고, 대조구의 어분을 육분, 콘글루텐 밀, 대두박 및 혈분으로 각각 다른 비율로 대체하여 어분 함량을 $40\%$ 전후로 감소시킨 실험사료들(사료 $2\~4$)을 설계하여 시판되고 있는 해산어용 배합사료 A, B, C (단백질 함량 : $51\~53\%$) 및 양어가들이 선호하는 RMP 사료(넙치용 분말사료와 냉동 메가리를 1 : 1의 비율로 혼합한 moist pellet, 단백질 함량 : $58\%$)와 그 효과를 사육 실험을 통해 비교하였다. 평균체중 125 g의 조피볼락을 선별하여 실험사료마다 2반복으로 15주간 사육실험한 결과, 최종 평균체중, 증체율, 사료효율, 일일사료섭취율, 일일단백질섭취율, 단백질효율 및 간 중량지수가 RMP 구를 제외한 실험사료와 상품사료를 급여한 실험구들은 대조구와 통계적인 차이는 없었다(P>0.05). RMP 섭취구의 증체율이 대조구와는 유의한 차이가 없었지만, 실험사료(사료 $2\~4$)와 상품사료(사료 $A\~C$)보다 유의적으로 높은 값을 보였다(P<0.05). 또한, 단백질효율은 RMP 구가 가장 높았고, 상품사료 B가 가장 낮은 값을 보였다. 대조사료에서 1 kg의 어체를 증체시키는데 소요되는 사료비용을 100으로 환산하였을 때, 사료 3 급여구는 92, 사료 4 급여구는 79로 나타나, 대조구보다 $10\~20\%$ 정도의 사료비가 절감되었고, RMP 사료 급여구는 대조구보다 $27\%$의 사료비가 더 소요되는 것으로 나타났다. 실험 종료시 간과 근육의 수분, 단백질, 지질 및 회분 함량은 모든 실험구간에 유의차는 없었다(P>0.05). A 15-week of growth trial was conducted to evaluate practical diets for growing Korean rockfish (Sebastes schlegeli). Two replicate groups of the fish average weighing 125 g were fed one of four experimental diets containing $41\~45\%$ protein and $6\~8\%$ lipid. A control diet with $58\%$ fish meal as protein source was included and practical form diets with $40\%$ fish meal and various practical ingredients such as meat meal, blood meal, soybean meal, and corn gluten meal were included. These experimental dry pellet diets were compared with commercial diets containing $51\~53\%$ protein and $5\~13\%$ lipid for marine fish or raw fish-based moist pellet (RMP) diet containing $58\%$ protein and $9\%$ lipid consisted of $50\%$ frozen horse mackerel and $50\%$ commercial binder meal. Weight gain, feed efficiency, daily feed intake and protein efficiency ratio in the fish fed the experimental diets and commercial diets were not significantly different to those in the fish fed the control diet. However weight gain, feed efficiency and protein efficiency ratio in the fish fed the RMP diet were significantly higher than those in the fish fed the experimental diets and commercial diets. These differences of growth performance between experimental diets and RMP diet may be reason for different dietary protein and energy levels. The experimental diet can save up to $20\%$ cost of fish production compare with control diet, whereas RMP diet is more expensive than other diets in the fish production.

지역특성을 고려한 학교규모 적정화 방향 설정에 관한 연구

이상민,조창희,Lee, Sang-Min,Cho, Chang-Hee 한국교육녹색환경연구원 2016 교육·녹색환경연구 Vol.15 No.1

본 연구는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석, 적정규모학교 가이드라인(안) 제안 등의 순서로 연구를 진행하였다. 적정규모학교 기준(안)은 시 도별 특성과 지역의 여건을 고려하며, 기존 선행연구들의 교육적 효과와 교육과정의 운영 효율성을 고려하여 이상적인 방향으로 제안하고자 한다. 본 연구에서는 적정규모학교의 효율적인 육성을 위하여, 적정규모학교에 대한 이론적 고찰, 국내 학생수 및 학교현황 분석, 적정규모학교 육성사업 현황분석을 통해 적정규모학교 가이드라인(안)을 제안하였다. School appropriate scale refers to the size of the school to minimize students' educational deficits and ensure an appropriate level of students, the number of classes, class size, etc., and maximize educational effects. Difficult set of absolute standards appropriate scale school, contemporary social situations and educational effects, and seek a comprehensive and reasonable direction, considering this is required. School appropriate scale it is preferable to consider the effect of conditions in areas such as education, curriculum, but it is common to set the range in terms of school operational effectiveness, and efficiency of education funding, school environment. So the concept of the appropriate scale including the school's status changes associated with school size, is seeking a new direction is needed so that the school environment, etc. are considered.