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돼지 수정란이식에 관한 연구 2 . 수정란의 회수 및 이식
이광원(K . W . Lee),손동수(D . S . Son),김상철(S . C . Kim),김일화(I . H . Kim),전대규(D . K . Jeon),유일선(I . S . Ryu),이종관(J . K . Lee),지설하(S . H . Chee),박창식(C . S . Park),석호봉(H . B . Seok) 한국축산학회 1988 한국축산학회지 Vol.30 No.7
This study of embryo transfer was carried out to investigate the possibility of introducing new genetic material into closed herds for disease control and of transporting embryos between countries. The results obtained were summarized as follows: 1. On the basis of the number of ovulation points counted, 69.1% of embryos were recovered. 2. A very high proportion (86.6%) of 1-cell embryos were recovered from the oviducts of donor sows at 37 to 41 hours after first mating. 3. Sixty-seven embryos were transferred to 5 recipients. Four recipients farrowed a total of 18 pigs, giving a survival rate of 26.9%. 4. Average pig weight at birth was 1.46㎏.
민감도 해석을 이용한 자동차 외부후사경의 진동 저감 설계
이광원(K.W.Lee),황광현(K.H.Hwang),박경진(G.J.Park),송운정(W.J.Song),이용호(Y.H.Lee) 한국자동차공학회 1997 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1997 No.6_2
The outside rearview mirror system is analyzed and designed to cnhance the vibrational behavior. The sensitivity information is utilized for the selection of design variables which are important in the bhavior of mirror system. With selected design variables, designs are determined using an orthogonal array and structural optimization approach. Also, the comparison test is carried out between the solutions of the orthogonal array method and the structural optimization.<br/>
이광원(K.W.Lee),황광현(K.H.Hwang),박경진(G.J.Park),송운정(W.J.Song),이용호(Y.H.Lee) 한국자동차공학회 1998 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1998 No.5_2
An automobile outside rearview mirror system has been analyzed and designed, to reduce the vibration, A finite element model is defined for the analysis with a commercial software. Modal analysis has been conducted for the calculation of natural frequencies. Harmonic analysis is utilized to estimate the displacements of the glass surface under dynamic loads, the model is verified with the vibration experiment for parts and the assembled body.<br/> The structure of the mirror system has been optimized for the robustness defined by the Taguchi concept. At first, many design variables are identified. Input design variables are selected due to the amount of contribution, the SN ratio in the Taguchi method is replaced by an objective function with mean and standard deviation, the defined objective function is appropriate in the structural design. The selected design minimized the vibration which it is insensitive to noise.<br/>
5ml 스트로에 보존한 돼지 액상정액의 생존성과 수정능력에 관한 연구
정행기(H . K . Chung),김학규(H . K . Kim),고문석(M . S . Ko),김인철(I . C . Kim),최진성(J . S . Choi),이광원(K . W . Lee),손동수(D . S . Son),김현(H . Kim),지설하(S . H . Chee),박창식(C . S . Park) 한국축산학회 1989 한국축산학회지 Vol.31 No.3
This experiment was carried out to test the efficiency of BF5 diluent in improving the length of maintenance of the fertilizing capacity of liquid boar semen in 5ml maxi-straw. The sperm-rich fraction of each ejaculate was slowly cooled to room temperature by 2 h after collection. Semen was diluted with BF5 diluent to provide about 20 x 10` sperm/ml. Semen was cooled in a waterbath to 51℃ over a 2-h period and BF5(+2 % glycerol) diluent was added to cooled semen(1:1). Straws were imm ediately filled with 5ml of semen and steel or glass balls were used to seal the ends of the straws. The straws were horizontally placed on a metal rack and stored at 51c in a refrigerator. At insemination the straw was rediluted with 75ml BTS diluent. The BF5 diluent maintained boar sperm fertilizing capacity for 9 days of in vitro storage. Conception rate, farrowing rate and number of pigs born alive per litter of diluted semen stored at 51℃ for 9 days were 70%, 50% and 9.6, respectively.
소의 동결수정란이 수태에 미치는 영향 2 . 자당부유액에 의한 2 단계 평형의 영향
석호봉,이광원,신용식,김호중,조윤연,오대균,지설하,임경순,알디 베이커 ( H . B . Seok,K . W . Lee,Y . S . Shin,H . J . Kim,Y . Y . Cho,D . G . Oh,S . H . Chee,K . S . Im,R . D . Baker ) 한국축산학회 1983 한국축산학회지 Vol.25 No.5
Twenty frozen embryos obtained from high genetically potential donors were introduced by cooperation with the American Holstein Association. The 47 recipients at the National Livestock Breeding Station were intramuscularly injected once or twice with 25㎎ PGF₂α for synchronization of estrus cycle with donors. The frozen embryos were rehydrated in 2-steps sucrose media after thawing at 37℃ and embryo transfer was performed by both of surgical and non-surgical methods. In addition, fresh embryos collected from 3 donors of the Station were transferred. The results were as follows: 1. Twenty-two of 23 fresh embryos obtained from 3 superovulated donors showed good morphology and average 7.3 embryos per donor were collected. 2. Among 47 heads treated, rate of estrus induced by twice of PGF₂α was higher (87.5%) than by once (78.7%), and the rate of heifers was higher than cows. 3. The quality of corpus luteum of 45 recipients synchronized in estrus was composed of 15 cows (33.3%) in excellent or good, 23cows (51.1%) in fair or little poor and 7 cows (15.6%) in no corpus luteum or cystic follicle, respectively. Most of recipients showed fair or little poor corpus luteum. 4. All frozen embryos except seven showed geneally good qualities in results of examination before transfer. 5. Twelve (40%) among 30 embryos were impregnated and all of them were Holstein breeds. 6. Two of 12 frozen and 10 of 18 fresh embryos were concepted, respectively. The conception rate of fresh embryo was higher than that of frozen embryo. 7. Forty-five percentage (rune of 20 recipients) of conception rate by non-surgical were higher than 30% (three of 10 recipients) by surgical method. 8. The surgical conception rates (66.7%) of fresh embryos were higher than non-surgical method (53.3%). However, surgical method (14.3%) of the frowns were lower than non-surgical (20.0%).
임재문,이광원,Lim, J.M.,Lee, K.W. 한국자동차안전학회 2012 자동차안전학회지 Vol.4 No.2
Prasad and Mertz published head injury risk curves for skull fracture and for Abbreviated Injury Scale (AIS) ${\geq}4$ brain injury due to forehead impacts based on the 15 ms HIC criterion. KNCAP adopted the HIC36 criterion for the male dummy and the HIC15 criterion for the female dummy. In this paper, it was studied that which of the HIC15 and HIC36 was more effective for the male dummy head injury evaluation. The frontal US-NCAP data for the 7 vehicles from the NHTSA test database were used to evaluate the head injuries. In the case of using the HIC15 and evaluation range 250~700, the discrimination of the rating for the occupant head injury was increased.
소의 동결수정란이 수태에 미치는 영향 1 . 그리세롤부유액에 의한 육단계평형의 영향
석호봉,이광원,신용식,김호중,조윤연,지설하,오대균,임경순,알피 엘스덴 ( H . B . Seok,K . W . Lee,Y . S . Shin,H . J . Kim,y . y . Cho,S . H . Chee,D . K . Oh,K . S . Im,R . P . Elsden ) 한국축산학회 1983 한국축산학회지 Vol.25 No.4
Nineteen frozen embryos with high genetic-potency were imported from Holstein Registration Association of America and transferred to recipients of the National Livestock Breeding Station. The synchronization was induced by twice intramuscular injections of 25㎎ PGF₂α with 14 days interval. The frozen embryos thawed at 37℃ were rehydrated within 6 steps in order to remove glycerol of the medium. The embryos were transferred to 19 recipients with non-surgical method and the results were as follows. 1. On 1st and 2nd intramuscular injection of PGF₂α, twentyeight (68.3%) and thirty-six (87.8%) among 41 treated showed estrus within 4 days after treatment. 2. The rate of the standing heat on day 3 after injection of PGF₂α was 53.7 and 73.6% at 1st arid 2nd injection, respectively. 3. The rate of the heifers showing standing heat after 1st and 2nd injection was 79.2 and 91.7%, but that of the cows was 52.9 and 82.4%. The rate was higher in heifer than cow. The rate of heifer selected for embryo transfer among synchronized recipients was also higher (62.5%) than that of cow (23.5%). 4 The evaluation of 19 frozen embryos (12 Holsteins and 7 Limousins) exception of 4 eggs with broken zona pellucida was generally good quality blastocysts as pre-freezing embryos. The pregnancy was obtained only from blastocyst. 5. At the examination of pregnancy at 60 days after nonsurgical transfers, three (15.8%) of 19 recipients were concepted. As the results, 2 female Holsteins (16.7%) and 1 male Limousin (14.3%) were delivered in terms of normal pregnant period.
수정란의 급속동결융해법에 관한 연구 1 . 마우스 동결수정란에 대한 1단계 Straw 법이 난자생존성에 미치는 영향
석호봉(H . B . Seok),이광원(K . W . Lee),전대규(D . K . Jeon) 한국축산학회 1991 한국축산학회지 Vol.33 No.1
This is a preliminary study to simplify freezing, thawing and transfer procedure of cattle embryos using mouse embryos. The experiments were designed to investigate the effective concentration of glycerol and sucrose by one-step straw method, and to determine the optimum thawing temperature. Effects of viability among permeable cryoprotec-tants and impermeable sugar dilutions was also studied. Results obtained were summarized as followed; 1. A total of 1,340 embryos were cryopreserved among which 1,390 embryos collected from 136 mice. 2. Post-thaw development to expanded blastocysts, was highest when embryos were frozen in 1.0 M glycerol+0.5 M sucrose. However, proportions of morphologically normal embryos was highest when they were frozen in 0.25 M glycerol+0.5M sucrose. 3. Post-thaw development to expanded blastocysts was highest when embryos were frozen in a lower concentrated sucrose compared to higher concentration. 4. The temperature thawed at 20℃ and 30℃ resulted in a significantly higher embryos survival rate after 72 hr. in culture than did at 35℃. However no significant differences was denoted between 20℃ and 30℃ (p$lt;0.05). 5. In effects of non-permeable sugar dilutions on in-vitro development of frozen-thawed mouse embryos, raffinose and trehalose were compared favorably with sucrose dilution in morphological recovery and survival rates. 6. When the embryos were frozen-thawed rapidly in 1.0 M glycerol and trehalose concentration, the proportions of embryos developed to expended blastocyst were significantly better in 1.25 M and 0.5 M than other higher concentration (p$lt;0.01). 7. In effects of permeable cryoprotectants and sucrose concentration, 1.5 M of permeable freezing media were significantly higher survival rate than that of 2.0 M and propanediol was resulted in slightly higher survival rate than glycerol. However, no significant differences showed among sucrose concentrations exception of null dilution(p$lt; 0.01). The results from this experiment suggested that the use of propanediol and raffinose in the rapid freezing technique offers slightly better results than does the use of glycerol and sucrose.
박창식(C . S . Park),이봉덕(B . D . Lee),지설하(S . H . Chee),이광원(K . W . Lee),이성호(S . H . Lee) 한국축산학회 1992 한국축산학회지 Vol.34 No.4
In order to solve the urgent manure disposal problem facing Korean swine industry, the wood sawdust fermentation floor system (WSF), was compared with the conventional concrete floor system (CCF) in a series of feeding trials. Trials 1 through 4 were carried out in a year, with 36 to 40 finishing pigs per trial, to investigate the performances during 4 different seasons on both CCF (4m×7m) and WSF (4m ×9m or 6m×8m). Pigs were group-fed with ad libitum feeding until they reached about 110㎏ BW. Initial and final BW were measured individually. The backfat thickness and loineye area were also measured at the final day of each trial using an ultrasonic instrument. Regardless of the season, no significant differences (P$gt;0.05) were found in daily gain between CCF and WSF. However, the daily gain of barrows showed that WSF was more advantageous than CCF during winter, and vice versa during summer. Except during winter, no significant differences (P$gt;0.05) were found in backfat thickness and loineye area between CCF and WSF. It turned out that WSF could be a way to solve the problems of manure disposal and malodours throughout a year without spending extra money or labor.