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소의 동결수정란이 수태에 미치는 영향 2 . 자당부유액에 의한 2 단계 평형의 영향
석호봉,이광원,신용식,김호중,조윤연,오대균,지설하,임경순,알디 베이커 ( H . B . Seok,K . W . Lee,Y . S . Shin,H . J . Kim,Y . Y . Cho,D . G . Oh,S . H . Chee,K . S . Im,R . D . Baker ) 한국축산학회 1983 한국축산학회지 Vol.25 No.5
Twenty frozen embryos obtained from high genetically potential donors were introduced by cooperation with the American Holstein Association. The 47 recipients at the National Livestock Breeding Station were intramuscularly injected once or twice with 25㎎ PGF₂α for synchronization of estrus cycle with donors. The frozen embryos were rehydrated in 2-steps sucrose media after thawing at 37℃ and embryo transfer was performed by both of surgical and non-surgical methods. In addition, fresh embryos collected from 3 donors of the Station were transferred. The results were as follows: 1. Twenty-two of 23 fresh embryos obtained from 3 superovulated donors showed good morphology and average 7.3 embryos per donor were collected. 2. Among 47 heads treated, rate of estrus induced by twice of PGF₂α was higher (87.5%) than by once (78.7%), and the rate of heifers was higher than cows. 3. The quality of corpus luteum of 45 recipients synchronized in estrus was composed of 15 cows (33.3%) in excellent or good, 23cows (51.1%) in fair or little poor and 7 cows (15.6%) in no corpus luteum or cystic follicle, respectively. Most of recipients showed fair or little poor corpus luteum. 4. All frozen embryos except seven showed geneally good qualities in results of examination before transfer. 5. Twelve (40%) among 30 embryos were impregnated and all of them were Holstein breeds. 6. Two of 12 frozen and 10 of 18 fresh embryos were concepted, respectively. The conception rate of fresh embryo was higher than that of frozen embryo. 7. Forty-five percentage (rune of 20 recipients) of conception rate by non-surgical were higher than 30% (three of 10 recipients) by surgical method. 8. The surgical conception rates (66.7%) of fresh embryos were higher than non-surgical method (53.3%). However, surgical method (14.3%) of the frowns were lower than non-surgical (20.0%).
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