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양희천(Hee-Cheon Yang),손희숙(Hee-Suk Son),심규광(Kyu-Kwang Shim),오찬호(Chan-Ho Oh),최동성(Dong-Seong Choi) 한국식품영양과학회 1992 한국식품영양과학회지 Vol.21 No.1
아그배로 부터 아세톤 침전, DEAE-cellulose 칼럼 크로마토그라피, Ultro-AcA 54겔여과의 과정을 거쳐 peroxidase를 분리 정제하고, 그의 특성을 조사하였다. 아그배peroxidase의 반응 최적 pH는 4.5, 반응 최적 온도는 80℃이었고, 30℃ 이하의 온도와 pH 5.0에서 안정하였으며, 80℃에서 15분간 보존했을 때 거의 불활성화되었다. OPDA에 높은 활성을 나타내었으나, phenol류 기질에 대해서는 약간의 활성을 나타내었으며, OPDA와 H₂O₂에 대한 Km치는 각각 1. 65mM, 7.97mM이었다. 아그배 peroxidase에 대한 저해작용은 L-ascorbic acid와 sodium L-ascorbate가 가장 컸고, 금속이온 중 Mn^(2+)만이 5mM 농도에서 효소의 활성을 증가시켰다. Peroxidase in the fruit of Malus sieboldii (Regel) Rehder was partially purified by DEAE-cellulose column chromatography and Ultro-AcA 54 gel filtration. The optimum pH of peroxidase was 4.5 and optimum temperature was 80℃. The enzyme was stable at pH 5.0 and below 30℃, and inactivated by heat treatment at 80℃ for 15min. In the presence of 30mM H₂O₂ Km value on o-phenylenediamine as substrate was 1.65mM, and in the presence of 10mM o-phenylenediamine Km value on H₂O₂ was 7.97mM. L-Ascorbic acid and sodium L-ascorbate greatly inhibited the enzyme activity and among several metal ions Mn^(2+) only increased the activity at 5mM.
食鹽濃度에 따른 간장덧 熟成過程中 化學成分의 變化에 대하여 ― 揮發性 有機酸을 中心으로 ―
양희천(Hee Cheon Yang),김병용(Byung Yong Kim),이태규(Tae Kyoo Lee) 한국식품영양과학회 1982 한국식품영양과학회지 Vol.11 No.3
食鹽濃度를 달리하여 在來式 메주와 改良植 메주를 이용한 간장덧 熟成過程중 色度, pH, 總窒素量, 還元糖量 및 揮發性 有機酸量의 消長 관계를 살펴본 바 다음과 같은 結果를 얻었다.<br/> (1) 色度와 pH는 在來式 간장덧보다 改良式 간장덧이더 낮았다.<br/> (2) 總窒素量을 改良式 간장덧이 在來式 간장덧보다 높았으며, 食鹽濃度가 낮을 수록 많았다.<br/> (3) 還元糖量은 改良式 간장덧이 在來式 간장덧보다 많았으며, 4주째에 最高値를 나타냈다.<br/> (4) 總揮發酸量은 高農度食鹽구에서는 초기에 점차 감소하다가 4주 이후부터 증가하였고 低濃度食鹽구에서는 처음보다 월등히 많아졌으며, 改良式 간장덧의 경우 揮發酸 중 acetic acid가 가장 많았다.<br/> (5) 在來式 간장덧에서는 改良式 간장덧에서 보다 butyric acid가 18배, propionic acid가 19배 많이 검출되었으며 食鹽濃度가 낮을 수록 두 有機酸의 생성이 많았다. With different salt concentration (18, 22, 26 & 30%), the changes of the chemical components for fermentation of the Korean native and modified soy sauce were investigated. Color intensity (absorband and pH were lower in the modified soysauce than in the native one. Total nitrogen content was high in the modified soysauce and with the lower salt concentration. Reducing sugar and total nitrogen were produced more in the modified soysauce. Reducing sugar attained to the maximum content at 4 wee and thereafter decreased. Total volatile acid in the higher salt concentration was produced much in the initial stage and decreased until 4 weeks, but slowly increased thereafter. Acetic acid showed the higher yield in the modified soysauce. Butyric acid and propionic acid were produced 18 and 19 times more the native soysauce than in the modified soysauce, and those were produced much with the lower sa concentration.
상수원수 전처리를 위한 생물여과의 막 특성에 관한 연구
강용태(Yong Tae Kang),양희천(Hee Cheon Yang),김승진(Seung Jin Kim) 한국수처리학회 2001 한국수처리학회지 Vol.9 No.2
This Paper evaluates the characteristics of the biofilm attached in biofiltration process for a pretreatment of surface water. The removal rate and activity of microbe was tested with respected to the variation of EBCT during start up period and stead-state period. The test was conducted with EBCT value varying -30% to +10% from the stead-state EBCT of 42 minutes. In start up period, removal rate was improved by decreasing EBCT to 20%(33.6 minutes, batch 2) from stead-state period EBCT(42 minutes, batch 4). The efficiency of KMnO₄ Consumption was improved to 22% and was maintained over 13 days. Turbidity was improved to 13.4% and was maintained over 11 days while Color and UV_(254) were improved to 12.5% and 23.6%, respectively. Color and UV_(254) were maintained over 15 days. The result of experiment indicated that adequate acclimation period under 20% reduced EBCT condition is 3 days when the biofilm formed media is used. In this experiment estimate activity for the attached microbe was found that the batch 2(EBCT 33.6 minutes) had 1.96 folds as many protein as batch 4 because decrease of EBCT led to improve activity of attached microbe.
쇄석여재를 이용한 생물막여과 공법에 의한 상수원수 전처리
강용태(Yong Tae Kang),양희천(Hee Cheon Yang),송근관(Kune Kwan Song),현길수(Kil Soo Hyun) 한국수처리학회 1999 한국수처리학회지 Vol.7 No.2
The Nakdong river being used as drinking water source for Pusan and Kyungnam province was over third grade in water quality criteria as BOD at lower reaches of the river. It is due to high organic loading from the each tributary of the river which had high nutrient concentration such as phosphorus and nitrogenous organic matter in the effluent from existing wastewater treatment plants, which depends on conventional activated sludge process. Thus, this research is to investigate and evaluate the characteristics of biological aerated filter process(BAF) as alternative pretreatment process in water treatment system. The results show that the BAF process was very effective in removing ammonia nitrogen in water, which the removal averaged more than 90%. The thermal coefficient of nitrification was 1.068 on the BAF.
강용태(Yong Tae Kang),양희천(Hee Cheon Yang) 한국수처리학회 1996 한국수처리학회지 Vol.4 No.4
The Nakdong river being used as drinking water sources for Pusan metropolitan city has contaminated due to inflow of inadequately treated wastewater from the tributaries into the river. In order to predict the characteristics of the river according to the variations of the flow and contaminants loading, water quality of the Nakdong river for a year was simulated by QUAL2E. The intensive surveys for the characteristics of the river section from Dalsung to Mulgum the downstream end of the Nakdong river were conducted during December 1995 to November 1996. The results of this study were summarized as follows. 1. In the lower reaches of the Nakdong river under Hanam the velocity of flow was quite slow like a lake. In case of COD the levels in Mulgum was from 7.0㎎/ℓ to 11.2㎎/ℓ and these values turned out to be fifth grade in the water quality criteria of the lake water. 2. The Nakdong river is a river in which the coefficient of riverbed that is presented as the ratio of maximum flow to minimum flow is too large. During the period of investigation the maximum value for the instance of a branch stream was 1:487.3 in the point of Milryang river and for a main stream, 1:124.6 in the point of Samryangjin. Therefore, the phenomenon of water shortage was too heavy in a period of dry season. 3. In order to improve the quality of the river the Master Plan of the all area of the river basin needs over all. 4. T-N, T-P levels ranged from 4.123㎎/ℓ to 25.361㎎/ℓ and from 0.475㎎/ℓ to 2.458㎎/ℓ, respectively, in the middle and upper part of Nakdong river and due to these effects Chlorophyll-a in Mulgum area that is clean water resource of Pusan metropolitan city was very significant and ranged from 57.1㎎/㎥ to 209.5㎎/㎥. Accordingly the results have it that existing wastewater, livestock wastewater and urgently industrial wastewater treatment systems should be converted to advanced wastewater treatment system or wastewater reuse system. 5. In a wide water supply scheme of abstrating on the lower of Hapchun Dam, the usual abstraction of 100 million ㎥/day aggravates the water quality of Mulgum that is the water resource of Pusan metropolitan city. Therefore, in order to settle these problems, the optimum operating method after hanging Hapchun Dam into water supply exclusive dam should be examined so that water quality improvement could be achieved by supplying water for river maintenance. 6. It could be realized that the creation of Wichun industrial complex would make much worse the water quality in Mulgum in the value of BOD: 0.2㎎/ℓ, COD: 0.3㎎/ℓ. And also it will not be feasibable to introduce a perfect non-discharge system of industrial wastewater in current domestic situations because the development of water treatment technology for 100 percent of treated effluent reuse and the rise of cost of production were not under consideration. 7. It is very urgent as a short term counter measure to introduce pretreatment facility by way of Aerated Biofilm filtration for the water quality improvement of clean raw water of Pusan metropolitan city because a long period is needed to regenerates all the water of river. 8. Non-point sources in the area of the Nakdong river and contaminated sludge layer on riverbed should be analyized as soon as possible, and then the counter plan for the treatment removal scheme, which is included in the content of general Master Plan, also should be settle by the prior orders.
강용태(Yong Tae Kang),양희천(Hee Cheon Yang),김덕진(Duk Jin Kim) 한국수처리학회 1997 한국수처리학회지 Vol.5 No.2
In the water treatment plant, collected water was treated by precipitation, sedimentation -rapid sand filtration-chlorine disinfection it is supplied to each home by passing the water distribution system. But, the containment which was not completely removed in the existent water treatment process, is likely to cause the qualitative decline of treated water such as the multiplication of the microorganism. And, aging of the pipe is an important problem as an cause of the treated water qualitative decline. The investigation of the water quality change in the distribution system is important for control of treated water. This research reported the water quality change in distribution system, especially, about the place, pipe diameter change, pump station, distribution reservoir, and pre and post site of the storage tank on apartments or houses from H water treatment plant to SJ beach. The results of this study were summarized as follows. Chlorine injection quantify must be adjusted so that residual chlorine concentration may be become necessary minimum concentration, which prevent rising of THM and multiplication of the microorganism in the distribution system.
강남콩(phaseolus vulgaris L.)의 지방질 성분
권용주,엄태붕,송근섭,김충기,이태규,양희천,Kwon, Yong-Ju,Uhm, Tai-Boong,Song, Geun-Seoup,Kim, Choong-Ki,Lee, Tae-Kyoo,Yang, Hee-Cheon 한국식품과학회 1987 한국식품과학회지 Vol.19 No.6
강남콩의 지방질을 chloroform-methanol-water (1:2:0.8, v/v)의 용매로 추출하고 이를 silicic acid column chromatography에 의하여 중성지방질, 당지방질 및 인지방질로 분획한 다음 이들의 지방질 조성과 지방산 조성을 TLC, GLC에 의하여 분리 정량하였다. 강남콩의 총지방질 함량은 1.9%이었으며 이중 중성지방질은 48.2%. 당지방질은 7.5%, 인지방질은 44.3%이었다. triglyceride는 중성지방질중 64.6% 함유된 주성분이었으며 가장 함량이 높은 인지방질과 당지방질로서 phosphatidyl choline은 인지방질중 32.9%, esterified steryl glycoside는 당지방질중 38.8% 함유되어 있었다. 강남콩의 주요 지방산은 linolenic, linoleic, oleic, palmitic acid이었으며 특히 linolenic acid는 그 함량이 높아 총지방질의 경우 37.1%, 중성지방질의 경우 50.4% 함유되어 있었다. Lipids of kidney bean were extracted by the mixture of chloroform-methanol-water (1:2:0.8 v/v), fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography, and the composition of these lipid classes were determined by TLC and GLC. The lipid content of kidney bean was 1.9%, and the lipid was consisted of 48.3% neutral lipids. 7.5% glycolipids and 44.2% phospholipids. Triglyceride was the major component of neutral lipids (64.6%). The major glycolipid and phosphlolipid were esterified steryl glycoside (38.3%) and phosphatidyl choline (32.9%). The major fatty acids of kidney bean lipid were linolenic, linoleic, palmitic and oleic acid. Linolenic acid contents were very high to be 37.1% in total lipid and 50.5% in neutral lipid.