[지역취재보도 부문] 제178회 〈오송 참사 부실 대응 단독·심층 연속보도〉 KBS청주 이만영, 송근섭, 최승원, 박준규, 강사완 기자

송근섭 (사)방송기자연합회 2023 방송기자 Vol.74 No.-

Bacillus subtilis가 생산하는 비특이적 $\beta$-fructofuransoidase의 부분정제 및 특성

송근섭,엄태붕 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.5

Bacillus subtilis의 세포내 이눌라아제가 부분정제되고 그의 작용 모드와 일반적 특성이 조사되었다. 이 효소는 gel filtration에 의하여 분자량을 추정하였을 때 49,000이었고, 등진점은 5.2 이었다. 기질에 대한 친화성의 지표인 Km값은 설탕에 대해서는 10mM, 라피노오스에 대해서는 18mM 이었다. 이 효소는 산성쪽에서는 불안정한 단백질로서 pH6.6에서 최대 활성을 보였으며 최적온도는 10분간 반응시켰을 때 50'C였다. 이 효소의 작용모든는 이눌린같은 구조를 가지는 과당 중합체를 과당 끝부분으로부터 하나씩 잘라가는 exo-cleavage 형이었다. An intracellular inulase ( fJ-fructofuranoside fructohydrolase, EC 3.2.1.26) from Bacillus subtilis has been partially purified and its mode of action and general properties were studied. The enzyme had an apparent molecular weight of 49,000 as estimated by gel filtration and its pI point was 5.2. Substrate concentration studies showed an apparent Km of 10 mM for sucrose and of 18 mM for raffinose. The enzyme was an acid-labile protein with a pH optimum of 6.6. The optimum temperature was 50$^{\circ}$C. The enzyme acts on straight chain oligo- and poly-fructosides of the inulin series via a exo-wise cleavage mechanism, as well as on sucrose.

Kategorischer Imperativ Kants und die moderne Wertethik

송근섭 한국동서철학회 1993 동서철학연구 Vol.10 No.1

都市·農村地域 幼兒의 營養狀態와 運動能力에 관한 調査硏究

宋根燮 又石大學校 1988 論文集 Vol.10 No.-

The purpose of this study was to investigate and analyze the nutritional condition and physical fitness of korean children four to six ago. 984 children were evaluated according to Rohrer's index, kaup's index and performance in the standing broad jump and body support children were placed in groups by age and residency in either a large city, a mediumsized a small city of a rural area. Residency groups of the same age were compared to determine if there were diffrence in nutritional condition and physical fitness levels among the groups. An addition, the existence of a relationship between the nutritional condition and physical fitness was investigate. Student's t-test methods were used for all statistical analysis. The conclusion of the study were;large city children of age 5 and 6 had a significantly better nutritional condition than did 5 and 6 year old children from the other areas in male and female;the body mass of 5 year old large city children was significantly greater than 5 year olds from medium and small city in male and female, but no difference were found among the other age groups;no difference existed among the individual age groups in muscular strength; the power of 6 year old large city children was significantly greater than 6 year olds from med-ium and small city in male and female, but no difference existed among other age grups; and no relationship was found between nutritional condition and physical fitness.

화학적 변이에 대한 Cinnamaldehyde의 항돌연변이 작용기구

송근섭,한상배,최동성 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.3

Cinnamaldehyde의 항돌연변이 작용기구를 밝히기 위하여 E. coli B/r 및 K-12 계열의 다양한 DNA 수복결손주를 이용하여 4-NQO 및 MNNG에 대한 돌연변이 억제 효과 및 생존율 변화에 대하여 조사하였다. SOS response가 항상 발현되는 균주인 GW1107의 β-Gal 활성과 41℃에서 β-Gal을 합성하는 균주인 GW1060 및 GW1103의 β-Gal 활성에도 cinnamaldehyde는 효과를 나타내지 못하였다. 이와 같은 결과는 cinnamaldehyde가 LexA와 같은 repressor로서 작용하지 못함은 물론 SOS response를 positive하게 조절하는 RecA 기능에 대하여 아무런 변화를 주지 못한다는 사실을 제시한다. 또한 Trp^+ 복귀돌연변이주의 생육 및 세포생장 속도에도 영향을 미치지 못하였다. 4-NQO 처리된 균주(WP2s, ZA159 및 TK603)에서 cinnamaldehyde의 첨가로 돌연변이율이 감소되었음에도 불구하고 생존율은 크게 향상되었다. 그러나 절제수복기능이 결여되지 않은 WP2 및 lexA 유전자 결손주(CM561 및 CM611)에서는 그와 같은 효과가 나타나지 않았으며, 상보적 DNA의 gap을 연결시켜주는 polymerase I이 결여된 균주인 WP67에서도 생존율이 증가되지 않았다. 위와 같은 결과를 종합하여 볼 때 cinnamaldehyde는 오류없는 재조합 수복계를 향상시킴으로써 항돌연변이 효과를 나타내는 것으로 추정되었다. The antimutagenic mechanism of cinnamaldehyde on mutagenesis induced by 4-nitroquinoline-1-oxide (4-NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was investigated in various DNA repair-deficient strains, E. coli B/r and K-12 series. Cinnamaldehyde did not show any effects not only on the β-galactosidase activities of GW1060 and GW1103(recA441) which synthesizes β-galactosidase consitutively at 41℃ but also on that of GW1107[lexA51 (Def)] in which the SOS response always occur. These results suggest that cinnamaldehyde does not change the function of RecA which positively controls the SOS response as well as not acting as the repressor like LexA. In addition, no inhibitory effect of cinnamaldehyde was observed on the growth of Trp^+ revertant and the delay of viable cell growth was also not found by adding cinnamaldehyde. Despite the decrease in the number of revertants, a significant increase in survival of 4-NQO treated cells was observed in E. coli WP2s (uvrA), ZA159 (△uvrB) and TK603 (uvrA). But these effects disappeared in excision-proficient strain WP2 (uvrA^+) and lexA-deficient strains(CM561 and CM611). The enhancement of survival was not found in WP67 (uvrA polA) deficient in polymerase I which ligates the gap between complementary DNA. From the above results, we assume that cinnamaldehyde might show antimutagenic effect by enhancing an error-free recombinational repair system.

종교철학의 가치론적 연구

송근섭 한국동서철학회 1989 동서철학연구 Vol.6 No.1

Studies on Fungal Lipids Containing γ - Linolenic Acid - 1. Fatty Acid Composition of Mucor sp.

송근섭(Geun-Seoup Song),김충기(Choong-Ki Kim),권용주(Yong-Ju Kwon),양희천(Hee-Cheon Yang),이태규(Tae-Kyoo Lee) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.6

감마-리놀렌산 생산능이 있는 새로운 곰팡이 균주를 탐색하기 위하여 토양으로부터 감마-리놀렌산 생산능이 있는 3개의 곰팡이 균주를 분리하였으며, 이들 균주를 진탕 배양을 행하여 균체성장, 지질생성량 및 지방산조성을 비교 조사하였다. 3가지 균주중에서 FA-007 균주성장도 양호하였고, 지질함량이 건조균체중 21.1%, 감마-리놀렌산 함량이 총지방산중 15.6%로 높은 특성을 나타내었다. 이 균주는 형태학적 특성을 조사한 결과 Mucor sp.로 동정되었다. 이 균주가 생산하는 지질은 중성지질이 75.2%, 당지질이 5.3% 및 인지질이 19.5%로 구성되어 있었고, 중성지질과 인지질에서의 감마-리놀렌산 함량은 각각 15.5% 및 18.3%를 차지하였다. For a study on the production of y-linolenic acid(GLA) by fungi, 3 fungal strains were isolated from soil. Their cell growth, lipid content and fatty acid composition were compared in shake flask culture. Among these fungi, the fungus, designated as FA-007, has high lipid content(21.1%) and GLA content(15.6% of total fatty acids). The fungal strain FA-007 was tentatively identified as Mucor sp. on the basis of morphological characteristics. Fungal oil produced by this fungus was composed of 75.2% neutral lipid, 5.3% glycolipid and 19.5% phospholipid. Although the GLA content in phospholipid was higher than it in neutral lipid, the GLA content in neutral lipid was high as 15.5%.

간접변이원의 돌연변이원성에 대한 생약재 열수 추출물의 효과

송근섭(Geun-Seoub Song),안병용(Byung-Yong Ahn),이갑상(Kap-Sang Lee),맹일경(Il-Kyung Maeng),최동성(Dong-Seong Choi) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.6

모델-제품범주 일치여부와 광고출연 유형에 따른 광고효과

송근섭(Song, Keun-Sop),김관규(Kim, Kwan-Kyu) 동국대학교 사회과학연구원 2013 사회과학연구 Vol.20 No.3

경쟁적인 광고 환경 속에서 유명인 모델은 다양한 제품의 광고에 중복하여 출연한다. 본 연구는 모델-제품범주 간의 일치도(일치 vs. 불일치)와 모델이 추천하는 제품의 수(단일 vs. 중복)가 광고효과에 어떤 영향을 미치는지에 대해 체계적으로 검증하였다. 실험 1은 스포츠 스타 유명인이 여러 제품들을 중복하여 추천하는 상황에서, 모델-제품 범주가 일치할 때가 그렇지 않을 때 보다 더 높은 광고효과를 보인다는 것을 검증하기 위해 설계되었다. 실험 결과, 제품범주가 일치하는 조건에서 더 높은 모델 신뢰도, 전문성, 친숙도를 보였으나, 모델의 호감도는 통계적으로 유의한 차이가 없었다. 제품범주가 일치하는 조건에서 더 높은 광고태도, 제품태도, 구매의도를 보였다. 실험 2는 제품범주 일치 조건을 통제(즉, 모든 광고제품을 스포츠 제품으로)한 뒤, 한 명의 스포츠스타 모델이 여러 제품을 중복하여 추천하는 조건과 단 하나의 제품을 추천하는 조건이 광고효과에 어떠한 차이를 보이는지를 검증하기 위해 설계되었다. 실험 결과, 중복출연 조건은 단일출연 조건보다 더 높은 모델 신뢰도, 친숙성을 보였다. 그러나 모델의 전문성과 호감도는 통계적으로 유의한 차이가 없었다. 이와는 다르게, 단일출연 조건은 중복출연 조건보다 더 높은 광고태도, 제품태도, 구매의도를 보였다. 결론적으로, 유명인이 중복출연 하는 광고는 모델과 제품의 범주가 일치할 때 더 효과적이며, 단일 출연모델이 더 높은 광고효과를 가져오지만, 중복출연은 모델의 신뢰도와 친숙성을 높여줄 수 있다. Commercials featuring celebrities have long been popular and in a competitive marketplace like these days, some celebrities might appear in several commercials in different product categories. Taking this into consideration, this study mainly focused on the effect of celebrity endorsement in advertising. Specifically, it aimed on investigating the effect of commercial model-product’s categorical congruency (congruency vs. in-congruency) and the number of products that the commercial model is endorsing (single vs. multiple) on consumer’s general responses. Experiment 1 was designed to test whether the advertising is more effective when celebrity athlete endorses multiple products with categorical congruency than with in-congruency, meaning that commercials with athletes endorsing sports-related products are more appealing than non-sports related products. The results of experiment 1 revealed the higher scores for commercial model’s credibility, expertise, and familiarity in the categorical congruency condition than in the categorical in-congruency condition, however, the commercial model’s favorability scores did not meet the significant difference. In addition to this, it also showed higher scores in attitude towards the advertisement and product together with purchase intention in the categorical congruency condition. Expanding on these results, experiment 2 was aimed at investigating the advertising effect of the multiple product endorser and the single product endorser in product’s categorical congruent condition. Participants were exposed to equal number of commercials in both conditions. In the multiple product endorser condition, all commercials were featured by single athlete, while in the single product endorser condition, each commercials were featured by different athletes. The results from experiment 2 showed higher scores for model’s credibility, and familiarity in the multiple product endorser condition than the single product endorser condition, however, the scores for model’s expertise nor favorability did not reach statistical significance. In contrast to this, the scores for purchase intention, attitude towards the advertisement and the product were higher in the single product endorser condition than the multiple product endorser condition.

미나리(Oenanthe stolonifera DC.)의 향기성분

송근섭(Geun-Seoup Song),권용주(Yong-Ju Kwon) 한국식품영양과학회 1990 한국식품영양과학회지 Vol.19 No.4

미나리(Oenanthe stolonifera DC)의 향기성분을 상압 수증기 증류법으로 추출하여 GC 및 GCMS로 분석한 결과 63가지 성분이 분리되었고, 그중에서 탄화수소류 20종(61.94%), alcohol류 9종(8.76%), ester류 1종(1.34%), aldehyde류 1종(2.29%), ketone류 3종(11.52%) 및 miscellaneous 5종 (2.35%) 등 39가지 성분을 동정하였다. 미나리 향기성분은 주로 탄화수소류(61.94%)로 구성되어 있었으며, 주성분은 limonene(12.12%), pulegone(9.48%), germacrene D(8.34%) 및 β-pinene(7.68%)등이었다. The volatile fraction of Oenanthe stolonifera DC. was extracted by a steam distillation under the atmospheric pressure and analyzed by gas chromatography(GC) and gas chromatography/mass spectrometry(GC-MS). The experimental results revealed the presence of 63 volatile components. Among them 39 components identified were composed of 20 hydrocarbons (61.94%), 9 alcohols(8.76%), 3 ketones(11.52%), 1 ester(1.34%), 1 aldehyde(2.29%) and 5 miscellaneous(2.35%). The major volatile components of Oenanthe stolonifera DC. were limonene(12.12%), pulegone(9.48%), germacrene D(8.34%) and α-pinene(7.68%).