사모로 살아가는 기쁨

양미희 두란노 2004 목회와 신학 Vol.- No.183

어떤 사모님은 목사 사모가 제일 힘든데 사모는 열 가지 일하면 열 가지 흉이고, 일 안하고 가만히 있으면 일 안 한다는 한 가지 흉을 잡힌다고 얘기를 하시더라고요. 그래서 그 말도 일리는 있구나 생각했어요. 하지만 너무 그렇게 소극적으로 앉아 있기보다는 교회 상황에 맞춰서 일을 적당히 하는게 좋은 것 같고 다만 사모가 너무 일선에 나서서 교회 일에 좌지우지하는 것은 좋지 않은 것 같아요. 사모는 있는 듯이 없고, 없는 듯이 있어야 한다고 생각해요. 사모가 나서서 적극적인 일을 하기 보다는 교회의 보이지 않는 부분들, 환자들이라든지, 고난을 당한 사람들을 도우는게 좋은 것같아요. 우리 교회만 해도 중보기도실에 가보면 기도 제목이 다 올라와 있거든요. 거기에서 왠만한 성도들의 다 알 수 있으니까 저는 알게 되면 저 성도가 아프구나. 어렵구나. 그러면 주일날 가서 아무 소리하지 않고 딱 손잡아 주면 성도들이 위로를 받는 것 같아요. 그게 효과가 있는 것같아요.

물 중 미세플라스틱 분석방법 표준화 연구

양미희,최인철,손보영,정아름,정지혜,이수정,서민정,박상민,박준원,윤정기,이수형 한국분석과학회 2023 학술대회논문집 Vol.2023 No.5

한국인에서 CYP2A6 유전자다형과 흡연 및 SCE의 상관성 연구

양미희 대한암예방학회 2007 Journal of cancer prevention Vol.12 No.2

The genetic polymorphism of cytochrome P450 (CYP) 2A6 has been emphasized to induce variations in smoking behavior, and in susceptibility to smoking-related cancer. However, there were a few studies concerning the CYP2A6 polymorphism in Korean populations. Therefore, focusing on presence of the CYP2A6 null allele (CYP2A6*4), I studied distribution of the CYP2A6 polymorphism in a Korean population (n=100) and its effects on sister chromatid exchange (SCE), a responsive biomarker for smoking or carcinogens. As results, the subjects who had the homozygous mutant type, the CYP2A6*4/*4, occupied 5.4% of the all subjects and most of them belonged to nonsmokers. However, the CYP2A6 polymorphism was not associated with the SCE levels (p=0.25), which were related to sex, smoking, and alcohol drinking. Therefore, presence of the CYP2A6 null allele may influence smoking behavior, however, it may be not involved in SCE-medicated cancer process. (Cancer Prev Res 12, 75-78, 2007)

DNA 마이크로어레이를 이용한 내분비장애 물질 노출지표 개발

양미희 환경독성보건학회 2005 환경독성보건학회지 Vol.20 No.4

장기간 노출 시 발암 등 인체 유해성을 갖는 환경유래 내분비장애물질(endocrine disrupting chemicals,EDCs)에 대한 선택적이고 민감한 노출지표를 개발하기 위하여 본 연구에서는 DNA microarray를 이용하였다. 피험자는 아직 특별한 질환을 갖지 않는 18세 이 상 연 령, 성을 맞춘 EDCs 고농도 노출군(N=16)과저농도군(N=16)으로 구성되었다. 노출정도 구분은 10년 이상 거주지가 K산업폐기물 소각장과 2.5km 반경 내, 외 인지에 따라 고노출군, 저노출군으로 구분하였다. 피험자의 말초혈에서 total RNA를 분 리, 각 군당 3인씩 pool로 cDNA를 합성하여 oligonucleotide DNA 칩에 적용하였다. 유전자발현의 차이를GenePixPro 4.0 software를 이용하여 분석하였다. 총 3장의 칩을 이용하여 공통적으로 저노출군보다 고노출군에서 2배 이상 발현의 증가를 보인 유전자는 plasminogen activator(PLAT) 등 12종이 관찰되었고, 1/2이하로 발현의 감소를 보인 유전자는 kallikrein 3(KLK3) 등 29종이었다. 이 들 유전자는 PLAT 등 면역계반응에 관여하는 유전자 및 암화, 발생(development), 대사 등에관여하는 유전자들이었다. 그러므로 KLK3 등 본 연구에서 발굴한 유전자는 향후 확대된 인구에서 본 연구 결과의 확인을 통하여 EDCs 특이적 노출지표로써, 나아가 암 등 EDCs관련 질병의 기전 및 병인학을구명하는데 이용가치가 높다고 사료된다.

[서평 (3)] 성숙한 신앙으로 삶을 살아 내기 위한 한 이성적 접근

양미희 대한기독교서회 2015 基督敎 思想 Vol.676 No.-

소아 중환자실에서의 진정제 사용 현황과 금단현상과의 상관관계 분석

양미희,배혜정,박지언,정애희,정선회,한현주,조윤희,조윤숙,최유현,박준동 한국병원약사회 2020 병원약사회지 Vol.37 No.1

Background : Although sedatives are commonly used in intensive care units, they may cause side effects such as delayed weaning of mechanical ventilation and withdrawal syndrome. There is no adequate assessment tool for evaluating the level of sedation in children and there is a lack of studies related to the use of sedatives in children. The purpose of this study was to investigate the use of sedatives in the pediatric intensive care unit (PICU) and to analyze the frequency and related factors of withdrawal syndrome. Methods : From January 1 through June 30, 2016, we retrospectively reviewed the electronic medical records of pediatric patients admitted to the PICU at the Seoul National University Children’s Hospital. The use of sedatives, tapering status, and frequency of withdrawal syndrome was investigated. Results : Ninety-three patients were treated with sedatives in the PICU at the Seoul National Children’s Hospital. Sixty-six were surgical patients, and 27 were medical patients. A combination of midazolam and fentanyl were the most commonly used sedative and analgesic (n=78, 83.9%). The median period of sedation was 60.1 hours (interquartile range (IQR) 75.8) in the surgical patients and 117.5 hours (IQR 121.2) in the medical patients. Compared to the surgical patients, long-term sedation was applied in medical patients. Tapering off of sedatives was performed in 40 patients (43.0%). The median total duration of tapering of sedatives was 16.0 hours (IQR 26.5) in the surgical patients and 30.2 hours (IQR 62.0) in the medical patients. The withdrawal syndrome occurred in 33 patients (37.1%) among 89 patients using midazolam as the main sedative. The duration of midazolam infusion was significantly associated with the frequency of withdrawal syndrome (Spearman’s rho 0.345, p=0.049). Conclusion : The withdrawal syndrome of sedatives was associated with the duration of sedation. Thus, more attention should be focused on tapering and monitoring the occurrence of withdrawal syndrome in patients requiring prolonged sedation.

한국인에서 DPD 및 TS, MTHFR의 유전자다형

양미희 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

In-order to do efficient treatment of 5-Fluorouracil (5-FU) in Koreans, we investigated genetic polymorphisms of dihydropyrimidine dehydrogenase (DPD; gene, DPYD), thymidylate synthase (75), and methyltetrahydrofolate reductase (MTHFR), which are involved in 5- FU metabolism or efficacy in a Korean population. As results, we found genetic polymorphisms in DPYD-85, -1627, and -1896 site, TS-5'ER, and -3'U'TR, and MTHFR-222, 429 site among the Koreans (normal, N=105; head and neck cancer patients, N=28). 5-FU efficacy was determined into PR(partial response, decrease of cancer size over 50%) and SD (stable disease). Each or combination of the above 7 genetic polymorphisms were not associated with 5-FU efficacy. Therefore, the studied 7 genotypes can not be considered to result in individual variation in 5-FU efficacy.

한국산 배(梨)를 중심한 과수의 기능성 연구

양미희 한국원예학회 2006 한국원예학회 학술발표요지 Vol.2006 No.5

In my previous study, consumption of Korean pears had inverse association with level of urinary 1-hydroxypyrene (1-OHP), which is an exposure biomarker and metabolite of polycyclic aromatic hydrocarbons (PAHs). Bioproduction of reactive intermediate of PAHs or induction of oxidative stress have been suspected as the mechanisms of PAHs-related diseases such as lung cancer, asthma, etc. Thus, to clarify potential of functional food in the Korean pears against PAHs-exposure, I studied effects of the Ko rean pears on 1. PAHstoxicokinetics, 2. PAHs- induced oxidative stress in intervention trials (N=30; male, N=10; female, N=20), and 3. PAHs- related chronic obstructive pulmonary disease (COPD: N= 30). The intake of Bulgogi, Korean barbeque, was used as exposure to PAHs. After 3 Ieks trials for the same subjects (1 week for Bulgogi only, Bulgogi with the pears, and the pears only, respectively), I found rapid excretion of urinary 1-OHP with the pears during 24 hrs compared to that without pears (0.05<p<0.1). In addition, I analyzed urinary levels of malondialdehyde (MDA), a biomarker for oxidative stress, following time cmyse. As results, I found that the Bulgogi induced MDA and the pears reduced the Bulgogi- induced MDA. I also analyzed another biomarker for oxidative stress due to PAHs, i.e. change in dichlorodihydro fluorescein (DCF). As a result, the DCF levels Ire not affected by intake of Bulgogi or the pears. HoIver, intake of the pear (half of a pear per day for 1 month) did not show significant improvement of ‘the St George’s respiratory questionnaire score’ or ‘forced expiratory volume for 1 second (FEV). In conclusion, my results suggest that the Korean pears have chemoprevention effects on PAHs- induced carcinogenic mechanisms due to rapid excretion of PAHs- intermediates and reduction of PAHs- induced oxidative stress. However, the pear-chemopreventive effects against PAHs-related diseases should be further clarified in enlarged population studies.

한국인 다기관 암 코호트 시료의 DNA 생활성도 평가

양미희,유지현,김청식,신애선,강대희,장성훈,박수경,신해림,유근영,Yang, Mi-Hi,Yoo, Ji-Hyun,Kim, Cheong-Sik,Shin, Ae-Sun,Kang, Dae-Hee,Chang, Soung-Hoon,Park, Sue-Kyung,Shin, Hai-Rim,Yoo, Keun-Young 대한예방의학회 2003 예방의학회지 Vol.36 No.4

Objectives : Peripheral blood-buffy coat fractions (N=14,956) have been stored at $-70^{\circ}C$ in the headquarter of the Korean Multicenter Cancer Cohort (KMCC), since 1993. To study the future molecular etiology of cancers using specimens of the cohort, properly stored specimens are necessary, Therefore, the DNA-viability of the bully coat samples was investigated. Methods : Buffy coat fraction samples were randomly selected from various collection areas and years (N=100). The DNA viability was evaluate from the UV-absorbent ratios at 260/280nm and the PCH for $\beta$-globin was performed with genomic DNA isolated from the buffy coat. Results : PCR products were obtained from 85 and 98% of the C and H area-samples, respectively, using 50 or $100{\mu}l$ of the buffy coat. There were significant differences in the yields of the PCR-amplifications from the C and H areas (p<0.05), which was due to differences in the homogenization of the buffy coat fractions available as aliquots. The PCR-products were obtained from all of the samples (N=7) stored at the C area-local confer, but the other aliquots stored at the headquarter were not PCR-amplified, Therefore, the PCR products in almost all the samples, even including the DNA-degraded samples, were obtained. In addition, an improvement in the DNA isolation, i,e. approx. 1.6 fold, was found after using extra RBC lysis buffer. Conclusions : PCR products for $\beta$-globin were obtained from nearly all of the samples. The regional differences in the PCR amplifications were thought to have originated from the different sample-preparation and homogenization performance. Therefore, the long term-stored buffy coat species at the KMCC can be used for future molecular studies.

대학생의 결혼관에 대한 연구

양미희,권혜성 명지대학교 1986 家政學硏究 Vol.2 No.-