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Lactobacillus spp. 와 Bifidobacterium spp. 의 Pulsed Field Gel Electrophoresis 에 의한 Genomic DNA 의 특성
윤영호,백영진,이재후 ( Y . H . Yoon,Y . J . Baek,J . H . Lee ) 한국축산학회 1998 한국축산학회지 Vol.40 No.1
Characteristics of genomic DNA of Lactobacillus spp. and Bifidobacterium spp. were determined by pulsed field gel electrophoresis and the following results were obtained; Four enzymes, Sma I, Apa I Not I and Sfi I were identified as suitable for generation of relatively few numbers of distinct fragments from the Lactobacillus spp. genomes, whereas five enzymes, Sma I, Apa I, Sfi I and Dra I were suitable for Bifidobacterium spp. genomes. The genome sizes of Lactobacillus spp. ranged from 1.S8Mb to 3.31Mb, the genome sizes of L. brevis ATCC 8287, L. fermentum ATCC 14931, L. casei YIT 9018, L. plantarum KCTC 1048 and L. delbruekii subsp. delbruekii ATCC 9469 were estimated to be 1.93Mb, 1.98Mb, 2.84Mb, 2.81M6 and 1.82Mb, respectively. Those of Bifidobcxterium spp. ranged from 1.85Mb to 2.83Mb. The genome sizes of B. bifrdum ATCC 29521, B. longum ATCC 15707, B. infantis ATCC 15697 and B. bifidum CU 1370 were estimated to be approximately 1.85Mb, 2.83Mb, 1.86Mb and 1.94Mb, respectively.
허정원,김정호,백영진,정기철,이용규 ( J . W . Huh,J . H . Kim,Y . J . Baek,K . C . Chung,Y . K . Lee ) 한국축산학회 1990 한국축산학회지 Vol.32 No.5
This reseach was performed to estabilish gene marker and elucidate lactose utilization system of Lactobacillus casei YIT 9018. Lactose negative mutants(lac) were isolated by curing agent such as acriflavin(20 ㎍/ml), acridine orange(80 ㎍/ml), ethidium bromide(10 ㎍/ml) and elevated temperature(42℃). Frequencies of lac mutants of L. casei YIT 9018 on these curing agents were 3.03, 1.41, 3.22 and 0.35%, respectively. The lactose negative mutants isolated had no ability of lactose utilization. L. casei YIT 9018 was found to have one plasmid of approxymately 45 Mdal, which was lost readily by curing agent and its ability to utilize lactose was lost silmultaneously.
Lactobacillus casei LM -1 의 Bacteriophage 저항성 기작에 관한 연구
임광세(K . S . Lim),장영호(Y . H . Jang),백영진(Y . J . Baek),김현욱(H . U . Kim) 한국축산학회 1991 한국축산학회지 Vol.33 No.10
This study was carried out to elucidate the phage defense mechanism of Lactobacillus casei LM-1, the phage-resistant mutant of L. cased S-1, and to obtaine the basic knowledge for developing a better lactic starter bacteria resistant to the phages. The results obtained are summarized as follows ; 1. No differences were found in growth, acid production, and carbohydrate fermentation between L. casei S-1 and L. casei LM-1 in the MRS broth. and transmission electron microscopic studies of L. case; LM-1 revealed that cells were covered with the hairly capsular layer, that is presumed to cover up the phage receptor sites on the surface of the cells. 2. When the cells of L. casei S-1 were treated with mutanolysin, the adsorption rate of phage J-I and ø-I was decreased rapidly. In case of L. casei LM-l, the adsorption rate of phage J-1 and ø-1 was increased around 30 minutes after addition of mutanolysin and then decreased. 3. Based upon the knowledge obtained from enzymatie treatment; and electron micrographs of L. casei LM-I, it is safe to say that the phage-resistant harrier of L. case; LM-1 is the extension of the modified pep-tidoglycan layer of the normal cell wall. 4. Treatment of L. casei LM-1 with 3 kinds of enzymes (subtilisin. α-amylase, β-amylase) did not affect the low adsorption of phage J-I and ø-1 on the host cells. which tells the capsular layer of L. casei LM-1 was not hydrolyzed by these enzymes. 5. No plasmids of L. casei S-1 and L. easei LM-1 were found on agarose gel electrophoresis, which suggests that the gene responsible for the formation of capsular layer was possibly induced due to the chromosomal mutation.