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Lactobacillus spp. 와 Bifidobacterium spp. 의 세포벽 분해효소 Autolysin 의 특성
이재후,윤영호 ( J . H . Lee,Y . H . Yoon ) 한국축산학회 1998 한국축산학회지 Vol.40 No.1
Studies were carried out to investigate the nature of cell wall hydrolyzing enzymes of Lactobocillus spp. and Bifidobacterium spp. which were utilized as starter organisms for fermented dairy products, the following results were obtained; Thirteen out of twenty-one strains of Lactobacillus and Bifidobacterium strains showed intensive clear cell wall hydrolyzing zone around the colonies on MRS agar plate containing 0.2% lyophilized Micorcoccus luteus cells. Cell wall hydrolyzing enzymes were isolated by SDS-PAGE and the cell wall hydrolyzing bands were detected on M. luteus cell containing gels. Each strains varied in molecular weight of cell wall hydrolyzing enzyme and in the number of cell wall hydrolyzing enzyme. L. casei YIT 9018 possess a single cell hydrolyzing enzyme of 76kDa. Optimum temperature and pH of the cell wall hydrolyzing enzyme of L. casei YIT 9018 were 37℃ and pH 6.0. Monovalent cations inhibited the enzymatic activity at above 0.1M concentrations and divalent cations inhibited the enzymatic activity extensively.
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Lactobacillus spp. 와 Bifidobacterium spp. 의 Pulsed Field Gel Electrophoresis 에 의한 Genomic DNA 의 특성
윤영호,백영진,이재후 ( Y . H . Yoon,Y . J . Baek,J . H . Lee ) 한국축산학회 1998 한국축산학회지 Vol.40 No.1
Characteristics of genomic DNA of Lactobacillus spp. and Bifidobacterium spp. were determined by pulsed field gel electrophoresis and the following results were obtained; Four enzymes, Sma I, Apa I Not I and Sfi I were identified as suitable for generation of relatively few numbers of distinct fragments from the Lactobacillus spp. genomes, whereas five enzymes, Sma I, Apa I, Sfi I and Dra I were suitable for Bifidobacterium spp. genomes. The genome sizes of Lactobacillus spp. ranged from 1.S8Mb to 3.31Mb, the genome sizes of L. brevis ATCC 8287, L. fermentum ATCC 14931, L. casei YIT 9018, L. plantarum KCTC 1048 and L. delbruekii subsp. delbruekii ATCC 9469 were estimated to be 1.93Mb, 1.98Mb, 2.84Mb, 2.81M6 and 1.82Mb, respectively. Those of Bifidobcxterium spp. ranged from 1.85Mb to 2.83Mb. The genome sizes of B. bifrdum ATCC 29521, B. longum ATCC 15707, B. infantis ATCC 15697 and B. bifidum CU 1370 were estimated to be approximately 1.85Mb, 2.83Mb, 1.86Mb and 1.94Mb, respectively.
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