과학적인 보호지방 사용 전략!

박덕섭 한국종축개량협회 2000 種畜改良 Vol.22 No.1

Aspergillus oryzae 및 Formitella flaxinea 첨가제의 급여가 착유우의 유생산 및 반추위 발효특성에 미치는 영향

박덕섭,김용국 한국축산학회 1998 한국축산학회지 Vol.40 No.6

The effects of fungal additives, Aspergillus oryzae fermentation extract (AO) and Fomitella flaxinea culture (FF), on milk production and ruminal fermentation characteristics, pH, VFA concentration, and NH₃-N concentration, in lactating cows were compared. Nine Holstein cows in mid-lactation (140 days in lactating period) were assigned to one of three diets; control, AO and FF for 12 days for each three replication in a switchback design. Cows were housed in a stanchion barn and fed individually twice a daily for restricted feeding. Daily milk production from the cows fed AO (19.32㎏) and FF (20.89㎏) were higher than those from the cows fed control diet (17.64㎏), (P$lt;0.05). Daily 4% FCM production from the cows fed FF (20.67㎏) was higher than that from the cows fed control (17.95㎏) and AO (17.65㎏), (P$lt;0.05). Milk fat, milk protein and solid-non-fat contents of milk from the control cows (3.92%, 3.29% and 9.66%) were higher than those of milk form the cows fed AO (3.53%, 3.10% and 9.05?b) and lower than those from the cows fed FF (3.93%, 4.13% and 10.64%), (P$lt;0.05). Fat acid composition of milk fat from the cows fed fungal additives tended to be varied compared to that from the cows fed control diet. Ruminal pH from cows fed FF (6.58) was higher than those from the cows fed control diet (6.41) and AO (6.38), (P $lt; 0.05). Total VFA concentration for FF group (340.87 mM/ ℓ ) was higher than those for the control group (286.37 mM/ℓ ) and AO group (239.27 mM/ℓ ), ( P $lt; 0.05). Ruminal NH₃-N concentrations were similar between the control (15.35 ㎎/㎗) and additive groups (15.39 and 16.25 ㎎/㎗).

폭등하는 사료원료가격, 어떻게 대처할 것인가?

박덕섭,Park, Deok-Seop 한국사료협회 2004 飼料 Vol.3 No.1

TMR 사료의 국내산 원료 이용 극대화 방안

박덕섭,Park, Deok-Seop 한국단미사료협회 2004 사료산업 Vol.1 No.1

국제분과 : 이스라엘 낙농산업과 젖소의 TMR 사양에 관한 연구

박덕섭(D . S . Park),김영호(Y . H . Kim) 한국동물자원과학회 ( 구 한국축산학회 ) 2001 낙농산업과 기술 Vol.1 No.1

항생제 대체제(앤타시드-100)의 급여가 젖소 송아지 육성에 미친 영향

명윤아,박덕섭,이인덕,남명수,이형석,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was carried out to develope an antibiotic substitute with some feed additive ingredients; activated charcoal, microbial products( Saccharomyces cerevisiae), sodium bentonite and pyroligneous. Sixty Holstein male calves(control 30 and tested 30 calves) were assigned to one of two diets, control(containing commercial antibiotic) and treatment diet (containing antibiotic substitute) with three replicates(10 calves each). The experiment were carried out for 30 days. The daily weight gains were similar between control(1.01kg/d) and treatment groups(1.01kg/d), however feed requirement were lower for treatment calves (2.80kg) than control calves (3.24kg) (P<0.05). Also calves were more health for treatment calves than control calves for diarrhea and respiratory diseases occurrences. The ammonia concentration of feces were lower for treatment calves(2.67 ppm) than for control calves(6.33 ppm) (P<0.05). No statistical difference were found in blood substances between control and treatment calves(P>0.05). In conclusion, the calve performances were improved by substitute without commercial antibiotic additive. 본 연구는 항생제를 대체할 목적으로 동물체의 활성을 가져오는 원료로 알려져 있는 활성탄과 목초액, sodium bentonite, 홍삼박, 생균제를 선별하여 이들 원료로 구성된 항생물질 대체제를 개발하여 홀스타인 수송아지에 급여하였을 때 생산성에 미치는 효과를 규명하고자 수행하였다. 송아지의 일당증체량은 대조구(1.011kg), 처리구(l.0lkg)간에 유의적인 차이가 없었고(P>0.05), 사료 요구율은 대조구 3.24에 비하여 처리구 2.80로 처리구에서 유의적인 개선효과를 나타냈다(P<0.05). 설사 발생률은 동일하였으나 분 상태는 처리구가 양호하였고, 호흡기 질병은 처리구에서는 발생하지 않았다. 분 중 NH₃ 농도는 대조구(6.33 ppm)에 비하여 처리구(2.67 ppm)에서 뚜렷한 개선 효과를 보였고(P<0.05), 혈액 수치는 대조구와 처리구가 유사하였다. 항생물질 대체제를 급여한 처리구가 시판 항생제 첨가사료급여군(대조구)에 비하여 증체에서는 다소 낮은 성적을 나타냈지만 질병 발생 없이 유사한 성적을 낸 것은 대체물질이 가축 소화기관의 활성화, 사료의 소화율 증진 등에 영향을 나타내어 동물의 활력이 증진되므로 항생제의 사용 없이도 양질의 축산물을 생산할 수 있는 결과를 얻었다.

활성탄의 급여가 젖소 송아지의 성장 및 배설물의 Ammonia 함량에 미치는 영향

유선일,박덕섭,명윤아,이인덕,남명수,김용국 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

This study was carried out to investigate the influences of feed containing activated charcoal on the performances of calves The daily weight gain, feed intake, feed requirement and the content of ammonia in the excreta were measured. The experiment was conducted with 10 male calves for 30 days. Each were equally divided with treatment group and control group and for the treatment group's animal fed diets containing 1.0% activated charcoal. The daily weight gain of calves were decreased by 1.92% when they were fed activated charcoal(1.02㎏/day) compared to that of control calves(1.04㎏/day) and the daily feed intake of treatment group(2.86㎏/day) was 10.06% lower comparing to the control group(3.18㎏/day). In case of feed requirement, the result showed 8.2% lower in the treatment group(2.80㎏) than control group(3.06㎏). The content of ammonia in excreta was significantly lower for treatment group(0.33ppm) than control group (2.67ppm)(p<0.05), which showed more improvement for reducing ammonia content in the treatment group with 87.6%(p<0.05). 본 연구는 사료에 활성탄을 첨가했을 때 송아지의 일당 증체량, 사료 섭취량, 사료 요구율 및 배설물의 ammonia 함량에 미치는 영향을 규명하기위해 실시하였다. 홀스타인 수송아지 10두를 30일 동안 대조구, 시험구(활성탄 1%첨가)로 각5두 공시하여 시험하였으며, 실험 결과를 요약하면 다음과 같다. 송아지의 일당 증체량은 대조구(1.04kg/일)에 비하여 활성탄을 급여했을 때(l.02kg/일) 1.92% 감소하였고, 일당 사료 섭취량은 시험구(2.86kg/일)가 대조구(3.18kg/일)에 비해서 10.06% 낮았으나, 사료 요구율은 대조구(3.06kg/kg증체)에 비해 시험구(2.80kg/kg증체)에서 8.2% 낮은 결과를 나타내었다. 송아지 배설물의 ammonia 함량은 대조구의 2.67ppm에 비하여 시험구가 0.33ppm으로 87.6% 감소되는 개선효과를 보였다(p<0.05).

육성초기 한국재래산양 사료로서 아까시나무 파쇄물의 영양가치

강병호,박덕섭,김건중,김용익,김용국 ( B . H . Kang,D . S . Park,K . J . Kim,Y . I . Kim,Y . K . Kim ) 한국축산학회 1998 한국축산학회지 Vol.40 No.2

Nine early growing Korean native goats were assigned in a randomized design to one of three diets containing of alfalfa hay(60%); alfalfa hay diet, alfalfa hay(30%) plus acacia wood chip(30%); acacia wood chip 50% diet, and acacia wood chip(60%); acacia wood chip 100% diet, of total ration to determine the nutritional value of acacia wood chip as a roughage source. Total mixed diet containing 60% roughages(alfalfa hay or acacia wood chip) and 40% concentrate were fed individually for 3 weeks. The daily gain, dry matter (DM) intake and apparent digestibilities of DM, energy and nutrients were determined. DM intake and daily gain were higher for goats fed alfalfa hay diet(637.8g/d and 54.1 g) than for goats fed acacia wood chip 50% diet(620.6g/d and 44.2g) and wood chip 100% diet(579.4g/d and 42.4g), however, those were not statistically different between groups(P$gt;0.05). Apparent digestibilities of DM, energy, and nitrogen were higher for goats fed alfalfa hay diet(73.00%, 72.03% and 77.32%) than for goats fed acacia wood chip 50% diet(64.75%, 61.90% and 64.75%) and wood chip 100% diet(58.33%, 54.77% and 58.77%)(P$lt; 0.05). Apparent digestibilities of fiberous components were higher for goats fed alfalfa hay diet(54.17%, 63.73% and 56.74%) than goats fed acacia wood chip 50% diet(42.08%, 51.79% and 44.64%) and acacia wood chip 100% diet (41.55%, 48.93% and 26.55%) (P$lt;0.05). However, those were not statistically different between acacia wood chip 50% diet and wood chip 100% diet. It could be concluded that acacia wood chip including leaves harvested during the summer could be a valuable roughage source for early growing Korean native goats.

착유우 사료에 대한 Aspergillus oryzae 발효물질 첨가가 in vitro 건물, 섬유소 및 단백질 소화율과 발효액의 pH에 미치는 영향

명윤아,박덕섭,이수기,박종수,김용국 충남대학교 농업과학연구소 2002 농업과학연구 Vol.29 No.2

본 연구는 Aspergillus oryzae 배양물이 in vitro 건물, 섬유소 및 단백질 소화율과 pH에 미치는 영향을 구명하고자 실시하였다. 균종 Aspergillus oryzae는 한국 생명공학연구원에서 분양받았으며, 본 연구에 사용된 사료는 시중 유통중인 착유우용 배합사료와 TMR 사료로서, 일반성분 분석은 (주)우성사료 중앙실험실에서 실시하였고, 건물, 섬유소 및 단백질 소화율 실험은 충남대학교 실험실에서 실시하였다. 배합사료와 TMR에 Aspergillus oryzae 배양물 0, 1.0, 2.0 및 3.0%를 첨가한 후, Holstein종 착유우로부터 채취한 반추위액에 시료 2.0g 내외를 넣어 진탕배양기에서 24, 48 및 72시간 소화시켰다. 침전물에 0.2% pepsin이 들어있는 0.1N HCl 30ml를 넣고 39℃ incubator에서 48시간 소화시키고 나서, 마지막으로 침전물을 60℃의 건조기에서 48시간 건조시켰다. 실험은 3회 반복하여 실시하였다. 건물 소화율은 Aspergillus oryzae 발효물을 첨가하지 않은 대조구에 비해 24시간 배양시킨 배합사료의 경우 2.1%(63.1%), 9.7%(68.5%) 및 9.0%(68%) 개선되었고, TMR은 4.8%(60.0%), 6.4%(61.1%) 및 2.9%(58.8%)의 개선효과가 있었다. 이와 대조적으로, 48시간 및 72시간 배양시킨 시험구의 건물 소화율에 Aspergillus oryzae 배양물이 미치는 영향은 24시간 배양시보다 상대적으로 낮은 경향을 보였다. 섬유소에서 소화율에서 Aspergillus oryzae가 조섬유, ADF 및 NDF 소화율을 유의적으로 개선시킨다는 것을 알 수 있었다. 배합사료에서 3% 첨가구의 경우 24시간 배양시 13.3%(53.3), 72시간 배양시 2.4%(54.6%)까지 증가되었다. 하지만, 첨가수준에 따라 소화율이 높아졌음에도 불구하고 첨가수준별 개선효과에는 유의적인 차이가 없었다. 단백질 소화율은 Aspergillus oryzae 배양물 첨가에 의해 배합사료에서 0.4%(79.7)에서 9.4%(71.8%)의 유의적인 개선효과를 나타내었지만, 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. TMR의 경우에는 4.0%(70.4%)에서 6.3%(65.1%)의 유의적인 소화율 향상을 나타내었지만 2.0%와 3.0% 첨가구간의 유의적인 차이는 인정되지 않았다. 본 연구에서, pH 처리구간 유의적인 차이는 없었으나, Aspergillus oryzae 배양물 첨가에 따라 pH가 약간 감소하는 경향을 보였다. 그러므로 본 발효물은 pH에는 영향을 미치지 않으나 건물, 섬유소 및 단백질 소화율 향상에는 다소 효과가 있는 것으로 결론을 내릴 수가 있다. This study was conducted to examine the effects of an Aspergillus oryzae fermentation culture on the in vitro digestibilities of dry matter, crude fiber, acid detergent fiber (ADF), neutral detergent fiber (NDF), crude protein and pH in vitro experiment of diets for dairy cows. A fungal species, Aspergillus oryzae was supplied by Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea (KCTC 1229). The experimental diets were commercial compound feed(concentrate) and total mixed ration (TMR) for lactating cows, of which chemical analyses were determined at Research and Development Institute, Woosung Feed Co.. Ltd.. while the digestibilities were done at the laboratory of Chungnam National University. Aspergillus oryzae culture products were added to compound feed and TMR at the rate 0, 1.0. 2.0, 3.0% respectively. The experimental diet with the rumen fluid sampled from Holstein fresian milking cows were used and digested for 24 hrs, 48hrs and 72hrs in the shaking incubator. The residues of the digesta were digested for 48hrs in the incubator in which put 30㎖ of 0.1N HCI with 0.2% pepsin at 39℃. The final precipitates were dried for 48hrs in the drier at 60℃. These experimental procedures were triplicated to determine the in vitro digestibility of dry matter. crude fiber. ADF, NDF, crude protein and pH. Compared to control diet, not added Aspergillus oryzae, the DM digestibility of fungal diets were improved 2.1%(63.1%), 9.7%(68.5%) and 9.0%(68.0%) for 24 hour fermentation in compound feed while 4.8%(60.0%), 6.4%(61.1%) and 2.9%(58.8%) in TMR. On the contrary. for 48 hour and 72 hour digestibilities, the effects of Aspergillus oryzae culture on the digestibility of dry matter were relatively lowered compared to 24 hour digestibility. Referring to the digestibility of dietary fiber, Aspergillus oryzae was believed to significantly improve digestibilities of crude fiber, ADF and NDF. Those were increased up to 13.3%(53.3%) for 24 hour fermentation while 2.4%(54.6%) for 3.0% added for 72 hour fermentation in compound feed. However, there were no signifiant differences among the treatments for the inclusion rate of Aspergillus oryzae, even though the more inclusion rate, the better digestibility. The protein digestibilities were significantly unproved from 0.4%(79.7%) to 9.4%(71.8%) by adding Aspergillus oryzae into compound feed. However, there were no significant differences between the two experimental diets. 2.0% and 3.0% Aspergillus oryzae included diets. In case of TMR, the protein digestibilities were significantly unproved from 4.0%(70.4%) to 6.3%(65.1%) by adding Aspergillus oryzae. However, there were no significant differences between the two experimental diets, 2.0% and 3.0% Aspergillus oryzae included diets. In this study, there were no significant differences among the treatments in pH. On the contrary, there were slightly decrease in pH by adding Aspergillus oryzae into experimental diets but not significant. Summarizing the results of this examination, Aspergillus oryzae fermentation culture is believed to improve the digestibilities of dry matter, fiber and crude protein in cattle diets. However, more detailed research for the mechanism of the fungal culture is required to improve ruminal environment.

수입 및 국내산 조사료의 사료가치 비교 연구

이형석,이인덕,박덕섭,금종수,박연진,김선균 한국초지조사료학회 2001 한국초지조사료학회 학술대회논문집 Vol.2001 No.6