Moutan Cortex Extract Inhibits Amyloid ${\beta}$ Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons

김주연,주현수,반주연,송경식,성연희,Kim, Joo-Youn,Ju, Hyun-Soo,Ban, Ju-Yeon,Song, Kyung-Sik,Seong, Yeon-Hee The Korean Society of Medicinal Crop Science 2008 한국약용작물학회지 Vol.16 No.6

Moutan cortex, the root bark of Paeonia suffruticosa Andrews (Paeoniaceae), has pharmacological effects such as anti-inflammatory, antiallergic, analgesic and antioxidant activities. We investigated a methanol extract of Moutan cortex for neuroprotective effects on neurotoxicity induced by amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to $10\;{\mu}M\;A{\beta}$ (25-35) for 24 h induced neuronal apoptotic death. Moutan cortex inhibited $10\;{\mu}M\;A{\beta}$ (25-35)-induced neuronal cell death at 30 and $50\;{\mu}g/m{\ell}$, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Moutan cortex inhibited $10\;{\mu}M\;A{\beta}$ (25-35)-induced elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) which were measured by fluorescent dyes. Moutan cortex also inhibited glutamate release into medium induced by $10\;{\mu}M\;A{\beta}$ (25-35), which was measured by HPLC. These results suggest that Moutan cortex prevents $A{\beta}$ (25-35)-induced neuronal cell damage by interfering with the increase of $[Ca^{2+}]_i$, and then inhibiting glutamate release and ROS generation. Moutan cortex may have a therapeutic role in preventing the progression of Alzheimer's disease.

Curcuma longae Radix, Phellinus linteus 및 Scutellariae Radix 혼합추출물의 $A{\beta}$ (25-35) 유도 배양신경세포독성 및 마우스기억손상 억제효과

김주연,정하연,반주연,유재국,배기환,성연희,Kim, Joo-Youn,Jeong, Ha-Yeon,Ban, Ju-Yeon,Yoo, Jae-Kuk,Bae, Ki-Hwan,Seong, Yeon-Hee 한국약용작물학회 2009 한국약용작물학회지 Vol.17 No.6

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid $\beta$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $10\;{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-50\;{\mu}g/m{\ell}$, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with HS0608 (25, 50 and 100 mg/kg, p.o. for 7 days) as measured by a passive avoidance test. From these results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer's disease.

백내장 수술 후 생긴 낭포황반부종에서의 유리체강내 트리암시놀론 주입술 일차치료 효과

김주연,김정목,유영주,김철구,조성원,이태곤.Joo Yeon Kim. MD. Joung Mok Kim. MD. Young Ju Lew. MD. PhD. Chul Gu Kim. MD. Sung Won Cho. MD. PhD. Tae Gon Lee. MD 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To report the clinical manifestations of intravitreal triamcinolone injection as a primary treatment method for patients with cystoid macular edema after cataract surgery. Methods: The present retrospective study was comprised of patients diagnosed with cystoid macular edema after cataract surgery performed between March 2006 and March 2008. To treat the edema, intravitreal triamcinolone injection was administered. Best corrected visual acuity (BCVA) and central macular thickness were measured, and complications as well as efficacy of the treatment were evaluated. Results: Twelve eyes were selected for the present study with a mean patient age of 67.3 years. The average period between cataract surgery and diagnosis of cystoid macular edema was 7.6 weeks. All 12 eyes received an intravitreal triamcinolone injection, and the patients were followed up for an average of 6.7 months. BCVA (log MAR) was changed from 0.29 ± 0.12 to 0.11 ± 0.09, showing statistically significant improvement (p = 0.000). The OCT showed that the average CMT was statistically decreased from 390.58 ± 94.98 μm to 165.17 ± 39.95 μm (p = 0.001). No complications were reported after the procedure, and vision and edema improved after one month in all 12 eyes. Conclusions: After performing intravitreal triamcinolone injection as a primary treatment method for cystoid macular edema which developed after cataract surgery, rapid recovery and improvement of vision and macular thickness were confirmed in all 12 eyes. J Korean Ophthalmol Soc 2012;53(3):428-433

독일문학의 맛에 매료된 세월

김주연,Kim, Ju-Yeon 대한출판문화협회 1997 출판저널 Vol.216 No.-

바라건데 언젠가는 18세기부터 오늘에 이르는 독일비평사 한권쯤 내 손으로 썼으면 한다. 이들을 읽을 때마다 그들의 예감과 통찰력에 나 자신 부끄러움을 느끼면서 언젠가 이들 수준에 이를 것인지 아득한 동경에 빠진다.

세기말 문학에 대한 안타까운 사랑-평론집 "상황과 인간"에서 "가짜의 진실, 그 환상"까지

김주연,Kim, Ju-Yeon 대한출판문화협회 1998 출판저널 Vol.237 No.-

나의 평론집에는 '인간'이라는 말이 늘 화두로 등장한다. 무엇 때문에 그토록 '인간'에 매달렸는지 모르겠는데, 결국 그것은 '인간'을 벗어나기 위한 몸부림의 과정이었다. 총체적 인간학인 문학의 보다 높은 원리를 갈구하던 나의 평론은 세기말 문학현상에 대한 안타까운 사랑이다.

흰쥐에서 녹차의 섭취가 ¹⁴C-Benzo[a]pyrene의 조직 분배 및 잔류에 미치는 영향

김주연,노상규,Kim, Ju-Yeon,Noh, Sang-K. 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.6

잔류성이 강하고 높은 지용성질을 가지는 식품오염물질인 benzo[a]pyrene(BaP)은 녹차 섭취에 의해서 소장에서 흡수가 억제되고 총담관으로의 배출이 증가되는 것으로 최근에 보고하였다. 이번 연구는 흰쥐를 이용해서 $^{14}C$-BaP를 복강주사한 후, 사람과 비교해서 1잔에 해당하는 녹차추출물을 4주간 매일 지속적으로 공급하였을 때, $^{14}C$-BaP의 조직분배 및 잔류에 미치는 영향을 조사하였다. 27.4 kBq $^{14}C$-BaP를 복강주사한 후 표준식이와 증류수만 공급된 동물군을 대조군, 표준식이와 녹차추출물(4.7 mg catechins/day)을 공급받은 동물군을 녹차군으로 하여 4주간 사육하였다. 그리고 4주째에 심장, 간장, 뇌, 비장, 신장, 후복막지방, 고환, 부고환지방 조직을 적출하여 무게를 측정하고 각 조직에 잔류하는 $^{14}C$-BaP의 방사선 활성도를 측정하였다. 대조군과 녹차군 모두 연구 시작부터 종료 시까지 지속적으로 체중이 증가하였으나 동물군 간에 유의적인 차이는 없었다. 장기의 무게 또한 각 조직 모두 동물군 간에 유의적인 차이가 없었다. 조직 g당 $^{14}C$-BaP의 잔류량은 심장에서 가장 높았고 다음으로 뇌, 부고환지방, 비장, 신장, 간장, 후복막지방, 고환 순으로 높았으며, 조직 전체의 $^{14}C$-BaP의 잔류량은 부고환지방에서 가장 높았고 다음으로 간장, 후복막지방, 심장, 뇌, 신장, 고환, 비장 순으로 나타났다. 녹차 섭취에 의해서 조직 g당 $^{14}C$-BaP의 방사선 활성도와 조직 전체 당 $^{14}C$-BaP 방사선 활성도 모두 심장, 간장, 뇌, 비장, 부고환지방에서 급격하게 감소된 것으로 나타났다. 이상의 실험결과들을 종합해 볼 때, 4주 동안 하루 1잔에 해당하는 녹차추출물 섭취가 BaP의 조직 분배를 유의적으로 감소시켜 조직 잔류에 영향을 미치는 것을 이 실험을 통해서 확인할 수 있었다. Recently, we showed that green tea extract (GTE) markedly lowers the intestinal absorption of $^{14}C$-benzo[a]pyrene ($^{14}C$-BaP) and enhances its secretion into the biliary route, suggesting a protective role for GTE against body burden. These findings indicate that green tea could be used as an effective dietary means against the toxicity of BaP. The present study, therefore, was designed to investigate if green tea intake could affect the tissue distribution and deposition of $^{14}C$-BaP in rats. Male Sprague-Dawley rats had free access to a nutritionally adequate AIN-93G diet and deionized water. At ~340 g of weight, the rats were injected intraperitoneally with 27.4 kBq of [4-$^{14}C$]-BaP and 5.0 mg of BaP dissolved in $300\;{\mu}L$ of olive oil and then assigned randomly to the following two groups: one group (GTE) of rats was fed the AIN-93G diet with GTE via drinking water at approx. 4.7 mg of catechins/d, whereas the other was fed the same diet but without GTE (control). At 4 wk of dietary treatment with GTE, animals were euthanized and heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat were collected, weighed, and analyzed for tissue $^{14}C$-BaP. Both the control and GTE groups continuously gained weight throughout the study, but there was no significant difference between the groups. No significant differences were observed in the weights of heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat. However, the radioactivities of $^{14}C$-BaP, expressed in dpm/g, were significantly lower in the heart, liver, brain, spleen, and epididymal fat of rats receiving GTE as compared to their respective controls. These data indicate that green tea intake markedly lowers tissue accumulation of $^{14}C$-BaP. Taken together, these findings suggest that the decreased tissue levels of BaP by GTE intake may be associated with lowered intestinal absorption of BaP and its enhanced secretion into the bile.

대학생 개인정보보호 인식조사를 통한 교육 개선방안 연구

김주연,Kim, Ju-yeon 한국정보통신학회 2019 한국정보통신학회논문지 Vol.23 No.3

개인정보의 수집과 사용이 지속적으로 증가하고 있는 추세에서, 개인정보를 보호하기 위한 법적 근거가 마련되고 각종 정책과 제도가 시행되고 있지만, 개인정보보호에 관한 교육과 그 효과에 대한 분석이 부족한 상황이다. 이에 본 연구에서는 대학생들의 개인정보보호에 인식정도와 실제 인지수준에 대해 분석하고, 개인정보보호 교육의 만족도를 조사하였다. 분석결과에서 학생들의 개인정보보호에 대한 관심이나 개인정보 노출 염려는 매우 높은 편이었으나, 실제 개인정보보호를 위한 노력이나 개인정보보호를 위한 권리 및 제도에 대한 인지수준은 매우 낮은 것으로 분석되었다. 또한 정보보호 교육을 받은 경험 및 만족도도 매우 낮은 것으로 조사되었다. 학생들은 개인정보보호 교육의 필요성은 크게 공감하였고, 일회성 교육보다는 정기적이고 지속적인 교육을 선호하는 것으로 분석되었다. In the trend of continuously increasing collection and use of personal information, there are legal grounds for protecting personal information and various policies and systems are implemented, but there is insufficient analysis about education and its effect of personal information protection. In this study, we surveyed and analyzed the degree of awareness and actual perception level in the protection of personal information of college students, and investigated the satisfaction of personal information protection education. The results of the analysis showed that students' interest in privacy protection and concern about personal information exposure was very high, but the efforts to protect personal information and the perception level of rights and systems for protecting personal information was very low. In addition, the experience and satisfaction of education for personal information protection were found to be very low. Students felt that the necessity of education for personal information protection was strongly agreed and preferred regular and continuous education rather than one-time education.

출판저널 칼럼

김주연,Kim, Ju-Yeon 대한출판문화협회 1991 출판저널 Vol.85 No.-

전해도금법으로 증착한 Cu-Sn 합금막의 배선특성에 관한 연구

김주연,배규식,Kim, Ju-Yeon,Bae, Gyu-Sik 한국재료학회 2002 한국재료학회지 Vol.12 No.3

Sn was selected as an alloying element of Cu. The Cu-Sn thin layers were deposited on the Si substrates by the electroplating method and their properties were studied. By rapidly thermal annealing(RTA) up to 40$0^{\circ}C$ after electroplating, sheet resistance decreased and adhesion strength increased, but that trend was reversed at the 50$0^{\circ}C$ RTA. Cu-Sn particles grew dense and the surface was uniform up to 40$0^{\circ}C$, but at 50$0^{\circ}C$, empty area was introduced and the surface became rough owing to oxidation and particle coarsening and agglomeration. Deposited layer contained significant amount of Si, while pure Cu-Sn layer with the composition ratio of 90:10 was present only on the top surface. However, no significant change in the Cu composition within alloy layers occured by the RTA regardless of its temperature. This indicates that the Cu diffusion into the Si was suppressed by the presence of Sn.

한파에 따른 표층수온의 지연시간 고찰 - 서해, 남해 -

김주연,박명희,이준수,안지숙,한인성,권미옥,송지영,Kim, Ju-Yeon,Park, Myung-Hee,Lee, Joon-Soo,Ahn, Ji-Suk,Han, In-Seong,Kwon, Mi-Ok,Song, Ji-Yeong 해양환경안전학회 2021 海洋環境安全學會誌 Vol.27 No.6

본 연구에서는 강한 한파가 발생했던 2018년과 온난 한파가 발생했던 2019년의 기온에 따른 수온의 반응 및 지연시간과 북풍계열 바람 빈도와의 상관관계를 분석하였다. 사용된 시간 자료는 국립수산과학원에서 제공하는 7개 지점 해역별 수온자료와 수온관측소 인근 7개 지점 AWS 기온자료를 이용하였다. 관측되지 못한 자료는 내삽법으로 근사값을 계산하였고, FIR Filter를 이용하여 자료의 주기성을 파악하였다. 그 결과, 강한 한파가 발생했던 2018년은 북풍계열 바람을 통해 차가운 공기가 남하하면서 기온을 하강시켜 전 해역에 저수온을 유발한 반면 온난 한파가 발생했던 2019년은 평년 수준의 기온으로 하강하였지만 수온은 크게 변화하지 않았다. 강한 한파가 발생했던 2018년 기온 하강에 따른 수온의 지연시간은 평균 14시간으로 0.7 이상의 높은 상관성을 나타냈고 온난 한파가 발생했던 2019년은 평균 지연시간이 20시간으로 0.44-0.67 사이의 상관성을 보였다. 본 연구를 통해 해역별로 기온 하강에 따른 표층수온의 반응을 해석하였고 지연시간을 파악함으로써 양식생물의 피해를 최소화하고 한파 피해의 신속한 대응에 기여할 수 것으로 기대한다. In this study, we examined the sea surface temperature (SST), air temperature (AT), and their time lag in response to an extreme cold wave in 2018 and a weak cold wave in 2019, cross-correlating these to the northern wind direction frequency. The data used in this study include SST observations of seven ocean buoys Real-time Information System for Aquaculture Environment provided by the National Institute of Fisheries Science and automatic weather station AT near them recorded every hour; null data was interpolated. A finite impulse response filter was used to identify the appropriate data period. In the extreme cold wave in 2018, the seven locations indicated low SST caused by moving cold air through the northern wind direction. A warm cold wave in 2019, the locations showed that the AT data was similar to the normal AT data, but the SST data did not change notably. During the extreme cold wave of 2018, data showed a high correlation coefficient of about 0.7 and a time lag of about 14 hours between AT and SST; during the weak cold wave of 2019, the correlation coefficient was 0.44-0.67 and time lag about 20 hours between AT and SST. This research will contribute to rapid response to such climate phenomena while minimizing aquaculture damage.