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김상주(Sang-Joo Kim),신준호(Joonho Shin),김윤재(Yun-Jae Kim) 대한기계학회 2002 대한기계학회 춘추학술대회 Vol.2002 No.3
A two-dimensional finite element model for polarization switching in ferroelectric materials developed by Kim and Jiang<SUP>(1)</SUP> and Sang-Joo Kim<SUP>(2)</SUP> is used to predict the formation of domains in a ferroelectric thin film. Initially the ferroelectric film is poled upward and the bottom electrode is grounded. A strong downward de field is imposed on a point of the top surface of the film. A region of switched domains with downward polarization is nucleated and is grown with time. It is found that initially the shape of the nucleated domains is that of a bulgy dagger, but later it turns into the shape of a reversed cup with the lower part wider than the upper part. It can also be concluded that the size of nucleated domains increases with the period of applied electric potential. The present results are qualitatively consistent with experimental observations.
원주방향 관통균열이 존재하는 배관의 J-적분 및 COD 계산을 위한 새로운 공학적 계산식
김윤재,허남수,김영진,Kim, Yun-Jae,Heo, Nam-Su,Kim, Yeong-Jin 대한기계학회 2001 大韓機械學會論文集A Vol.25 No.3
A new method to estimate the elastic-plastic J-integral and the crack tip opening displacement (COD) for circumferential through-wall cracked pipes under tension and under bending is proposed for Leak-Before-Break (LBB) analysis. Being based on the reference stress method with further modifications, the proposed method is simple to use and easy to be generalised in practice. Comparison of the CODs, predicted using the proposed method with published pipe test data show overall excellent agreement.
초고온 고세균 Thermococcus pacificus P-4로부터 내열성 β-glucosidase의 새로운 기질 특이성
김윤재,이재은,이현숙,권개경,강성균,이정현,Kim, Yun Jae,Lee, Jae Eun,Lee, Hyun Sook,Kwon, Kae Kyoung,Kang, Sung Gyun,Lee, Jung-Hyun The Microbiological Society of Korea 2015 미생물학회지 Vol.51 No.1
Thermococcus pacificus P-4의 유전체 서열 분석을 통하여 예측되는 GH1 ${\beta}$-glucosidase를 암호화하는 유전자를 동정하였다. 그 유전자는 487 아미노산들을 암호화하는 1,464 bp 나타내었으며, 그 아미노산 서열은 Pyrococcus furiosus ${\beta}$-glucosidase와 77% 상동성을 나타내었다. 그 유전자는 Escherichia coli 시스템 내에서 복제 및 발현하였다. 재조합 된 단백질은 금속 친화크로마토그래피를 통하여 정제하고 특성을 분석하였다. 정제된 단백질(Tpa-Glu)은 pH 7.5와 $75^{\circ}C$에서 최적활성을 나타내었으며, 열안정성은 $90^{\circ}C$에서 약 6시간의 반감기를 보였다. Tpa-Glu는 pNP-${\beta}$-glucopyranoside, pNP-${\beta}$-galactopyranoside, pNP-${\beta}$-mannopyranoside, 그리고 pNP-${\beta}$-xylopyranoside 순으로 우수한 $k_{cat}/K_m$ 값을 나타내었다. 또한, Tpa-Glu는 ${\beta}$-1,3-linked polysaccharide (laminarin) 그리고 ${\beta}$-1,3-와 ${\beta}$-1,4-linked oligosaccharides에 대하여 exo-hydrolyzing 활성을 보였다. 본 연구는 초고온 고세균으로터 ${\beta}$-glucosidase가 exohydrolyzing 활성을 처음 확인한 것으로 이 효소는 laminarin의 당화공정에 ${\beta}$-1,3-endoglucanase와 함께 적용할 수 있을 것으로 기대된다. Based on the genomic analysis of Thermococcus pacificus P-4, we identified a putative GH1 ${\beta}$-glucosidase-encoding gene (Tpa-glu). The gene revealed a 1,464 bp encoding 487 amino acid residues, and the deduced amino acid residues exhibited 77% identity with Pyrococcus furiosus ${\beta}$-glucosidase (accession no. NP_577802). The gene was cloned and expressed in Escherichia coli system. The recombinant protein was purified by metal affinity chromatography and characterized. Tpa-Glu showed optimum activity at pH 7.5 and $75^{\circ}C$, and thermostability with a half life of 6 h at $90^{\circ}C$. Tpa-Glu exhibited hydrolyzing activity against various pNP-glycopyranosides, with kcat/Km values in the order of pNP-${\beta}$-glucopyranoside, pNP-${\beta}$-galactopyranoside, pNP-${\beta}$-mannopyranoside, and pNP-${\beta}$-xylopyranoside. In addition, the enzyme exhibited exo-hydrolyzing activity toward ${\beta}$-1,3-linked polysaccharide (laminarin) and ${\beta}$-1,3- and ${\beta}$-1,4-linked oligosaccharides. This is the first description of an enzyme from hyperthermophilic archaea that displays exo-hydrolyzing activity toward ${\beta}$-1,3-linked polysaccharides and could be applied in combination with ${\beta}$-1,3-endoglucanase for saccharification of laminarin.
김윤재 ( Yun Jae Kim ),안경수 ( Kyeong Soo Ahn ),최진욱 ( Jin-wook Choe ),임진석 ( Jinseok Lim ),최성웅 ( Sung Woong Choi ) 한국복합재료학회 2024 Composites research Vol.37 No.2
지속적으로 증가하는 세계적인 전력수요로 인해 고전압 해저케이블 수요도 증가하고 있다. 해저케이블은 고가의 제작 비용과 높은 단가의 포설 비용 등이 해결되어야 할 부분 중 하나이다. 해양 환경에 노출된 해저케이블은 파랑 및 해류 등의 외력들에 의해 케이블 손상이나 사고 시에는 높은 유지 보수 비용 등의 문제가 발생할 수 있다. 따라서 해저케이블은 다양한 종류의 보호 기자재를 통해 해저 환경의 불확실성에 대비하고 있다. 본 연구에서는 대표적인 보호 기자재인 피기백 클램프(PBC)와 클램프에 사용되는 스트랩(strap)을 대상으로 해석적 방법을 통해 휨 성능을 살펴보았다. 피기백 클램프의 구조적 성능 검토를 위해 유연 보호 튜브의 최대 굽힘 모멘트 기준치인 15 kN·m 값을 토대로 휨 성능을 분석하였다, 그 결과, 피기백 클램프 및 strap의 굽힘 모멘트에 대한 휨 성능이 확보되었음을 확인할 수 있었다. Due to the continuously increasing global demand for electricity, the demand for high-voltage submarine cables is also increasing. One of the issues that need to be addressed for submarine cables is the high production cost and expensive laying costs. Submarine cables exposed to the marine environment encounter external forces such as wave and current, leading to issues such as cable damage due to external factors or high maintenance costs in the event of an accident. Therefore, we are preparing for the uncertainty of the submarine environment through many protective materials and protective equipment. In this study, we examined the bending performance of piggyback clamps (PBC) and strap, which are representative protective equipment, in response to the submarine environment through analytical methods. To examine the structural performance of PBC, the bending performance were assessed under the maximum bending moment criterion of 15 kN·m for the flexible protection tube. As a result, it was confirmed that the structural performance regarding the bending moment of both PBC and straps was ensured.
참조응력법의 표면균열의 비선형 파괴역학 해석 적용성 연구
김윤재(Yun-Jae Kim),김진수(Jin-Su Kim),심도준(Do-Jun Shim),김영진(Young-Jin Kim) 대한기계학회 2003 대한기계학회 춘추학술대회 Vol.2003 No.8
This paper discusses applicability of the enhanced reference stress method to estimate J along the semi-elliptical surface crack front. It is found that angular variations of normalised J are strongly dependent on the geometry, loading mode and loading magnitude. As application of the reference stress approach to semi-elliptical surface cracks implies proportional increases in the normalised J, the present results pose a question in applicability of the reference stress approach. However, it is also found that the reference stress approach can still provide reasonably accurate J results from an engineering point of view, provided that the maximum J along the crack front is accurately estimated. In this respect, investigation of the error in the estimated J in the present work suggests that the enhanced reference stress approach, recently proposed by authors, provides an effective engineering tool for estimating crack driving force along the semi-elliptical surface crack front.