Human a-Synucleins을 위한 형광표지 리포좀 결합분석법의 개발

김시경,최웅 충북대학교 의과대학 충북대학교 의학연구소 2004 忠北醫大學術誌 Vol.14 No.2

Purpose : To understand the mechanism of interaction between a-Synuclein and phospholipid membrane, a quantitative method analyzing the binding of a-Synuclein to the phospholipid membrane was invented with fluorescence-labelled phospholipid liposome and this method was applied to the human a-Synuclein mutants. Materials and Methods : The phospholipid liposome was made of cholesterol, phosphatidylcholine and phosphatidylserine. Wild type and pathogenic mutnats (A30P, E46K. A53T) of human a-Synuclein was expressed and purified as GST-fusion proteins. Liposome binding assay was done with phospholipid liposome and GST-fusion human a-Synucleins. Results : Liposome binding assay was optimised for several parameters such as the concentration of protein and liposome, incubation time and temperature. At this optimised condition, the strength of binding to the phospholipid liposome was observed as such A30P < A53T .wild type < E46K. Conclusion : Liposome binding assay invented and optimized in this study is expected to be a useful tool for examining the relationship between the structure and the function of a-Synucleins. 연구목적: Human a-Synuclein과 phospholipid membrane과의 상호작용기전을 이해하고자, fluorescence-labelled phospholipld liposome을 이용하여 a-Synuclein 의 phospholipid membrane bmding을 정량분석하는 기술블 개발하고, 이를 human a-Synuc1ein 의 mulant 에 적용 하고자 히였다. 대상 및 방법: Cholesterol. phosphatidylcholine, phosphatidyserine으로 구성된 phospholipid liposome을 만들어 GST-융합 재조합 단백질로서 발현된 human a-Synuclein의 wild type 및 pathogenic mutant (A30P, E46K, A53T)와의 binding을 측정하였다. 결과: 형광표지를 이용한 liposome binding assay를 개발하고, 단백질 및 phospholipid 농도, 반응시간 및 온도 등 여러 가지 조건을 최적화 하였다. 이 조건에서 human a-Synuclein의 phospholipid liposome에 대한 binding 강도는 A30P < A53T ~ wild tupe M < E46의 순서였다. 결론: 본 연구에서 개발한 liposome binding assay는 앞으로 a-Synuclein의 구조와 기능을 연구하는데 유용한 연구수단으로 이용될 수 있을 것으로 예상된다.

뉴라운드의 登場과 韓國의 對應方案 : 인터넷 라운드와 多者間 投資協定을 중심으로

김시경 단국대학교 1999 論文集 Vol.34 No.-

韓國自動車産業의 海外進出 活性化 方案에 관한 硏究

김시경,김창원 단국대학교 경영대학원 1995 經院論叢(壇國大學校) Vol.14 No.-

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

마그레브( 구 불령 북아프리카) 제국의 통상 및 투자 환경에 관한 연구-우리나라와의 관계 개선을 중심으로

김시경 한국중동학회 1998 韓國 中東 學會 論叢 Vol.19 No.1

식품 원료 농산물의 위생안전관리 방안에 대한 연구

김시경,박주연,김건희 덕성여자대학교 자연과학연구소 2006 자연과학 논문집 Vol.12 No.-

굴참나무천연림의 생산구조 및 물질생산력에 관한 연구

김시경,정좌용 ( Si Kyung Kim,Jwa Yong Jeong ) 한국산림과학회 1985 한국산림과학회지 Vol.70 No.1

Growth and biomass production of natural stands of Quercus variabilis in relation to tree density were studied to obtain basic guide lines for future tending operation. Two natural stands of Quercus variabilis located at 900m (A stand: 6,600trees/㏊, 15.84㎡/㏊, 19/17-20) and 800m (B stand: 4,300trees/㏊, 16.65㎡/㏊, 20/17-21) elevation in Sancheong, Kyongnam Province were selected for the comparative study and following results were obtained through a sample plot method, After diameter of individual trees in the sample plots was measured, twelve average trees from each diameter class were cut felled to measure dry weight of W_S, W_B, W_L, W_(Ba), and standing biomass and biomass production rates by a allometrior regressions related to D²H. Vertical distribution of leaves along the stems indicated that photosynthesis was carried out 2.2m above the ground in Stand A and 1.2m in Stand B. Maximum photosynthesis was located 4.2m and 6.2m above the ground in Stand A and B, respectively. Leaf area index was 4.25㏊/㏊ for Stand A, and 3.89㏊/㏊ for Stand B. Above-ground standing biomass was 49.51 ton/㏊ for Stand A and 59.20 ton/㏊ and net annual production was 6.75 ton/㏊/yr. for Stand A and 8.99 ton/㏊/yr for Stand B. The ratio of net annual production to standing biomass was 17.5% for Stand A and 16.7% for Stand B. Net assimilation rate was 2.75㎏/㎏/yr. for Stand A and 3.58㎏/㎏/yr.for Stand B. Stem wood production rate was 1.46㎏/㎏/yr. for Stand A and 2.09㎏/㎏/yr. for Stand B. Bark production rate was 0.60 ㎏/㎏/yr. for Stand A and 0.34㎏/㎏/yr. for Stand B. Above data indicated that Stand B utilized growing spaces and sites more efficiently than Stand A. It is concluded chat productivity of natural stands of Quercus variabilis can be enhanced through optimization of basal areas and number of tree per hectare and that sound management of natural oak stands should be based on systematic: sampling of the area for periodic productivity estimation.

정신분열병 환자의 흡연 특성 (Tobacco Smoking Characteristics in Patients with Schizophrenia)

김시경 충북대학교 의학연구소 2009 忠北醫大學術誌 Vol.19 No.1

-본문참고-

정신보건심판위원회 계속입원치료 심사지침 평가

김시경,김연실 대한신경정신의학회 2011 신경정신의학 Vol.50 No.6

Objectives The aims of this study are to test the applicability of the continuing hospitalization judgement guidelines of The Primary Mental Health Review Board and to provide the basic data for the development of valid guidelines. Methods The request sheets submitted to Cheongju Primary Mental Health Review Board for continuing hospitalization from April 2009 to July 2001 were reviewed. The results according to the guidelines were compared with board’s final decision. The aim in making these comparison was to determine the factors affecting the board’s decisions. Results Cheongju Primary Mental Health Review Board assessed 1223 requests during the period, and declined 65 of those requests. The sensitivity and specificity for dissent of the algorithm in the guidelines are 51.6% and 97.0%, respectively. The patterns of data in the sheets from the mental facilities were different from the psychiatric hospital or the clinic. The presence of a guidance’s signature, mental status, and specialist’s opinion could be factors affecting the board’s decision. Conclusion The result suggests that distinguished guidelines are needed for mental facilities. Social support systems and functional impairments may be meaningless because they did not affect the board’s decision or could be replaced by other factors. The algorithm and judgement system should be revised to increase sensitivity for dissent. Further research is required concerning the development of standardized guidelines on the judgement for continuing hospitalization.

플래시를 이용한 아이콘 언어 설계 및 구현

김시경 이화여자대학교 교육대학원 2005 이화교육논총 Vol.15 No.-

This study proposed an icon language as a medium for communication using pictures. An icon language is useful in overcoming the diversity of languages and occupies much less space than texts in expressing the same concept. In addition, it may be valuable for the disabled who have difficulties in communication and for infant education related to the development of creativity. However, as an icon language expresses using visual signs without spoken words, it is extremely difficult to describe complicated concepts. That is, because there are too many objects that are unreasonable to express with icons, we may have to use a large number of icons. Furthermore, in order to communicate intentions using icons, there have to be various syntactic rules. In order to overcome such shortcomings of an icon language, this study defined the grammatical rules of an icon language, designed icon sentences based on the grammatical frame to express meanings, and implemented an icon language editor. The icon language was designed through sentence design by sentence component, and the editor was implemented using a flash program in order to support various file formats with a low capacity and to provide many people with easy access. In addition, icons were designed to be practical, intuitive and distinct so that meanings expressed by them are not ambiguous but predictable by users.