LC-MS/MS를 이용한 육류 중 페니실린계 항생제 8종의 동시분석 및 적용성 검증

김명애,윤수진,김미경,조윤제,최선주,장문익,이상목,김희정,정지윤,이규식,이상재 한국식품위생안전성학회 2014 한국식품위생안전성학회지 Vol.29 No.2

The objective of this study was to develop a simultaneous method of 8 penicillin antibiotics includingamoxicillin, ampicillin, cloxacillin, dicloxacillin, nafcillin, oxacillin, penicillin G and penicillin V in meat usingLC-MS/MS. The procedure involves solid phase extraction with HLB cartridge and subsequent analysis by LC-MS/MS. To optimize MS analytical condition of 8 compounds, each parameter was established by multiple reaction monitoringin positive ion mode. The chromatographic separation was achieved on a C18 column with a mobile phase of0.05% formic acid and 0.05% formic acid in acetonitrile at a flow rate of 0.2 mL/min for 20 min with a gradient elution. The developed method was validated for specificity, linearity, accuracy and precision in beef, pork and chicken. The recoveries were 71.0~106%, and relative standard deviations (RSD) were 4.0~11.2%. The limit of detection(LOD) and the limit of quantification (LOQ) were 0.003~0.008 mg/kg and 0.01~0.03 mg/kg, respectively, that arebelow maximum residue limit (MRL) of the penicillins. This study also performed survey of residual penicillin antibioticsfor 193 samples of beef, pork and chicken collected from 9 cities in Korea. Penicillins were not found in all thesamples except a sample of pork which contained cloxacillin (concentration of 0.08 mg/kg) below the MRL (0.3 mg/kg).

간호관리료 차등제 주요 개정 내용 및 기대 효과

김명애,Kim, Myeong-Ae 대한간호협회 2007 대한간호 Vol.46 No.1

우유의 처리과정

김명애,Kim, Myeong-Ae 한국유가공협회 1983 牛乳 Vol.4 No.3

유탕처리된 쌀엿강정용 팽화쌀의 조직학적 연구

김명애 한국식생활문화학회 2001 韓國食生活文化學會誌 Vol.16 No.4

쌀엿강정은 쌀을 호화·건조하여 유탕처리하거나 가압 등으로 팽화시켜서 제조한다. 이때 호화과정에서 가열기구나 호화의 정도가 쌀의 팽화에 영향을 미칠 것으로 판단되어 이들 처리에 따라 팽화량(크기, 비중), 사진촬영, 주사형 전자현미경(SEM)으로 관찰·비교하였다. 1. 전기밥솥이나 압력솥, 혹은 증기로 찌는 등 밥의 형태로 쌀을 호화시킨 것은 팽화쌀이 서로 부착된 것이 많아 좋지 않았다. 전기밥솥이나 압력솥의 경우는 팽화쌀의 표면에 파괴된 부분이 나타났다. 압력솥이나 증기고 찌는 경우는 쌀의 팽화량이 작았다. 2. 쌀을 세정하여 바로 많은 양의 물로 끓이는 방법이 좋았으며, 쌀을 불려서 끓이는 방법과 팽화량에 차이를 보이지 않았다. 쌀을 불리더라도 익을 정도로만 단시간에 끓일 경우에는 오히려 팽화량이 작았다. 3. 쌀을 끓이는 시간이 길수록 팽화는 컸으나 너무 지나칠 정도로 끓일 경우에는 쌀의 표면이 파괴되거나 균열이 생겨서 외관이 좋지 않고 팽화량도 작았다. This study was carried out to observe the character change of expanded rice grain for Salyeotgangjung at various gelatinization methods. The expansion and structure change of rice grain was different at conditions of heating methods and gelatinization, respectively. The boiling showed good expansion compared to other heating methods by electric rice-cooker, pressure cooker, and steam cooker. There was not difference in expansion of rice grain between the boiling and the boiling after soaking. Rice grain did greatly expand as the boiling time was extended. But the quality for Salyeotgangjung would be not good because of crack of expanded rice grain surface when the boiling time was too long.

당뇨환자를 위한 콩가루와 보리가루 혼합찐빵의 품질

김명애,윤석권 동덕여자대학교 2003 생활과학연구 Vol.8 No.-

This study was conducted to investigate useful mixing amount of soybean and barley flour in making steam bread. The high adding amount of soybean and barley flour resulted from low expansion and high hardness of steam bread. Steam bread expanded well and gained high sensory evaluation score as 10% of soybean flour was added to the kneading of bread compared to the non-added treatment. But there were not significant difference in the quality of steam bread such as expansion, hardness and preference between non-added treatment and added treatment to 30% of soybean flour or 10% of barley flour.

離乳食에 대한 調査 : 濟州市內居住 영유아를 中心으로

金明愛 제주한라대학 1978 論文集 Vol.4 No.-

As random samples for this study, 150 married women were taken who lived in Jeju with their last live child aged under Five years. The Study was conducted during the period of July,1978.to August,1978. In the examination and analysis of the materials, it could be found that there were some tendencies and problems in infant feeding ways and weaning practice. The results are as follows: 1. 54.7 Percent of the infants were sufficiently fed by mothers milk. But, the other 45.3 percent were not enough to be breast fed. 2. 54.7 Percent of the mothers started the weaning before 6 months of age and the more the mothers' level of education is increased the time for weaning is fasted. 3. Reasons were dominant on the part of mother for taking artificial feeding (47.3%). They were, in detail, mothers job (26.0%), insufficient milk flow and mothers' health problem (20.7%) 4. Majorty of the mothers' preffered to give infants corns as the major diets. 5. The percentage of those who use nutritional supplements and nutritional medical preparations extremly low, and even more suprising is the fact that only 7.3% use iron preporation.

환자안전을 위한 투약간호원칙 준수활동

김명애,김영미,서영경,조정숙,유정숙,김금희,홍성은,최성혜,권원경,박승혜,서혜향,김옥수,한순묘,곽성숙,이숙경,김주희 한국의료QA학회 2006 가을학술대회 Vol.2005 No.2

Impaired Spindle Checkpoint Response of Brca1-deficient Mouse Embryonic Fibroblasts (MEFs) to Nocodazole Treatment

김명애,김현주,윤진호,Kim Myoung-Ae,Kim Hyunju,Yun Jeanho Korean Society of Life Science 2006 생명과학회지 Vol.16 No.1

항암유전자 Brca1의 변이는 유방암 및 난소암에 대한 감수성을 증가시키며, Brca1은 DNA손상신호후 세포주기 조절에 필수적인 역할을 한다. 연구결과, Brca1이 세포주기 S기와 G2/M 조절점에서 중요한 역할을 담당함이 밝혀졌다. 그러나, Brca1의 spindle checkpoint 관여여부는 알려져 있지 않다. 본 연구에서는 spindle checkpoint를 활성화시키는 nocodazole를 처리하여 야생형, $p53^{-/-}$ 그리고 $p53^{-/-}\;Brca1^{-/-}$ 세포주의 세포주기 변화를 조사하였다. 야생형과 $p53^{-/-}$ 세포주는 신속한 mitosis기 정지가 나타난 반면, $p53^{-/-}\;Brca1^{-/-}$ 세포주의 경우 모든 세포가 M기에서 정지하지 않았다. Double-thymidine block 기법에 의한 세포주기 동조화후 nocodazole 처리시에도 $p53^{-/-}\;Brca1^{-/-}$ 세포주에서는 일부세포가 M기 조절점을 통과하여 계속 G1기로 진행하였다. 형태학적 분석에서도 nocodazole 함유배지에서 계속 증식하는 세포형태가 관찰되었다. 이와 같은 결과들은 Brca1이 spindle checkpoint가 정상적으로 작동하는데 중요한 역할을 담당한다는 것을 의미하고 있다. Genetic alternation of Brca1 predispose of breast and ovarian cancer. Brca1 plays critical role in cell cycle regulation following DNA damage. Previous studies revealed that Brca1 plays an important role in S phase and G2/M checkpoint regulation. However, whether Brca1 involves in spindle checkpoint is unclear. In this study, the role of Brca1 in cell cycle response following nocodazole, which is a reagent that depolymerizes microtubules and activates the spindle checkpoint, has been examined using wild type $p53^{-/-}\;and\;p53^{-/-}Brca1^{-/-}$ mouse embryonic fibroblasts (MEFs). While wild type and Brca1-proficient MEFs showed an acute mitotic arrest, Brca1-deficient MEFs failed to arrest at mitotic phase in response to nocodazole treatment. In double-thymidine block and nocodazole treatment experiment, a portion of $p53^{-/-}\;Brca1^{-/-}$ MEFs were clearly by-passed nocodazole induced mitotic arrest. Consistent with this, in morphologic analysis, $p53^{-/-}\;Brca1^{-/-}$ MEFs showed growing cell morphology after nocodazole treatment. Taken together, these results suggest that Brca1 protein is an important component for normal induction of spindlecheckpoint and impairment of Brca1 function could induce dysregulation of mitotic cell cycle that ultimately results in genomic instability.

갱년기 여성의 성생활에 대한 조사

김명애,박신애 여성건강간호학회 1999 여성건강간호학회지 Vol.5 No.2

Due to the ever increasing life-span of human beings, the average woman is living well into her late 70's. Henceforth, they spend at least one-third of their life after menopause. Climacteric encompasses the time proceeding, during, and proceeding menopause : a transitional period of shifting from the period of being fertile to the period of senescence. In other words, this is the time at which they lose the ability to reproduce. Menopause can influence a woman's sexual life immensely due to the physiological, social, and psychological changes that occur during that period. In Korea, where some women still live according to Confucian Culture in terms of sexology, nurses can play a vital role in the dissemination of sexual facts to women. This study was designed to clarify the characteristic and satisfaction of sexual life. This was done according to the classification of three types of climacteric women: pre-menopausal, menopausal, and post-menopausal. I studied 159 climacteric women between the ages of 45 & 59, living in or around the vicinity of Taegu City. This study utilized the investigative tools for sexual life patterns which were constructed with field experts' consultation and reference review by the author. The sexual satisfaction was translated from DSFI. The climacteric symptoms were invented by Kupperman. This research is descriptive. The data was collected between July 15, 1998 and October 14, 1998. Statistical analysis was performed using ANOVA and Pearson Correlations, and was computed with the SPSS program. The results obtained are summarized as follows ; The frequency of sexual activity was highest in pre-menopause and declined after menopause. The frequency of genital caressing declined after menopause. The difference between the frequency of masturbation and coital pain was not significant according to the classification set forth in this experiment. Congruently, the frequency of orgasms declined after menopause. The mean satisfaction of sexual life is 27.1 years. The range of expected score is 9-45 years of age. Satisfaction was highest in the pre-menopausal group and proved to be statistically significant. Some of the general characteristics that affected the satisfaction a woman's sexual life in the climacteric stage during this project were; age, income, the symptoms of climacteric, etc. There was higher satisfaction in younger pre-menopausal and the larger income group than the other experimental groups. The climacteric symptoms were conversely correlated with the satisfaction in one's sexual life.

서울 및 안동 일부 지역주민의 식단 조사 : 대표식단의 혈당반응 조사를 위한 For Investigation of Glycemic Responses to Typical Domestic Meals

김명애,윤석권,한민수 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.3

한국인이 가장 많이 이용하는 식단을 알기 위하여 45가지 항목으로 된 설문서를 만들어 20세 이상의 성인들을 대상으로 조사하였다. 조사대상자는 20대부터 50대까지 고루 분포하였으며 수입은 100∼200만원의 54%로 중류 생활자가 주로 조사대상이 되었다. 잡곡은 80%이상의 사람이 이용하였고 10%미만은 섞거나 먹지 않았으며, 잡곡은 보리와 콩이 주류였고, 가정에서 밥 이외에 먹는 식사로는 비빔밥, 김밥이 많았다. 면류는 라면류와 칼국수를 주로 먹었으며 50%정도는 약간의 밥을 동시에 섭취하였고, 가정에서 빵류는 식빵을 제일 많이 이용하였다. 국은 약 81% 정도가 자주 먹거나 거의 먹었으며 제일 많이 먹는 국은 채소된장국, 미역국, 쇠고기국 순서였다. 찌개와 국은 87% 정도가 둘 중 하나만 상에 오른다고 하였으며 찌개는 된장찌개나 김치찌개를 많이 이용하였다. 반찬은 3∼4종류가 제일 많고 제일 자주 먹는 육류는 돼지고기와 쇠고기 구이였다. 생선류는 주로 구워서 제일 많이 섭취하였고, 김치는 99%가 매일 먹으며 가지수는 2가지 종류가 제일 많고, 종류로는 배추김치가 많았다. 나물류로는 콩나물과 숙주나물, 시금치, 쑥갓, 미나리나물을 많이 이용하였다. 외식에서 가장 많이 이용하는 것은 밥류에서 갈비탕 등의 탕류와 비빔밥, 면류에서 짜장면, 양식에 서는 돈까스, 빵류에서는 햄버거가 많이 이용되었다. The general meal intake survey in two regional areas(Seoul and Andong in Kyungsangbuk province) was conducted to investigate what the Korean favorite dishes are, as a part of study on their blood glucose responses. The survey was carried out to fill up the 45 kinds of questionaires to adults over 20 years old. The age of subjects was evenly distributed from twenties to fifties and 54% of them was middle class, whose monthly income was between a milliom won and two million won. The 72% of subjects mixed cereals(mainly barthly, soybean) with rice but about 28% did not. Domestic meals except general Korean meals were bibimbab and kimbab. Major noodles at home was ramen and kalgugsu(home made noodle) and 50% of the subjects took the noodle with small amounts of steamed rice. The 41% of subjects took the gug(Korean soup) in every meal and the major soup was vegetable doengang soup, sea mustard soup, beef soup and soybean sprout soup in order. The 87% of subjects took either soup or pot stew, and doenjang pot stew and kimchi pot stew were mainly used. The number of side dishes except soup, pot stew and kimchi was 3∼4. The side dishes in meat was cooked mainly with beef and pork. The favorite cooking type of fish is roastion one. Two kinds of kimchi were served in every meal and the major kimchi was cabbage kimchi, kkagdugi(radish cube kimchi), yulmu kimchi in order. The major meal of eating out was galbitang and bibimbab in Korean style, jjajangmyon in Chinese one, pork cutlet in western one and hanmburger as a fast food.