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김근형 ( Geunhyung Kim ) 한국정보처리학회 2009 한국정보처리학회 학술대회논문집 Vol.16 No.2
All IP 기반 BcN(Broadband Convergence Network)의 발전과 단말 기술의 발전은 언제 어디에서나 임의의 단말을 통해 웹과 같은 데이터 서비스, 통화 서비스, 스트리밍 서비스를 동시에 이용하는 것을 가능하게 하였다. 주로 TV를 통한 멀티미디어 콘텐츠 서비스가 PC와 모바일 단말 상에서도 가능해 졌으며, PC를 중심으로 사용되던 웹 기반 서비스가 TV와 모바일 단말에서도 사용이 가능해지고 있다. 본 논문에서는 서비스 이용환경을 향상시키기 위해 특정 단말에서 이용 중인 서비스를 다른 단말에서 끊김 없이 이용할 수 있도록 하는 서비스 세션 이동성을 보장하는 메커니즘에 대해서 살펴보고, 웹 기반 서비스의 세션 이동성 보장에 관한 기존 연구를 비교 분석하고, 향후 연구 방향을 정립하고자 한다.
A 3D bioprinting system and plasma-surface modification to fabricate tissue engineering scaffolds
GeunHyung Kim(김근형) 한국표면공학회 2017 한국표면공학회 학술발표회 초록집 Vol.2017 No.5
The achievement of tissue engineering can be highly depending on the capability to generate complicated, cell seeded three dimensional (3D) micro/nano-structures. So, various fabrication techniques that can be used to precisely design the architecture and topography of scaffolding materials will signify a key aspect of multi-functional tissue engineering. Previous methods for obtaining scaffolds based on top-down are often not satisfactory to produce complex micro/nano-structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. However, a bioprinting method can be used to design sophisticated 3D tissue scaffolds that can be engineered to mimic the tissue architecture using computer aided approach. Also, in recent, the method has been modified and optimized to fabricate scaffolds using various natural biopolymers (collagen, alginate, and chitosan etc.). Variation of the topological structure and polymer concentration allowed tailoring the physical and biological properties of the scaffolds. In this presentation, the 3D bioprinting supplemented with a newly designed plasma treatment for attaining highly bioactive and functional scaffolds for tissue engineering applications will be introduced. Moreover, various in vivo and in vitro results will show that the fabricated scaffolds can carry out their structural and biological functionality.
WonJin Kim(김원진),JiUn Lee(이지운),Gi-Hoon Yang(양지훈),Hyeongjin Lee(이형진),YongBok Kim(김용복),Minseong Kim(김민성),YoungWon Koo(구영원),GeunHyung Kim(김근형) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Collagen- based cell-printing technology has provided a new strategy for tissue engineering. However, although collagen-based scaffolds can provide an outstanding biofunctional benefits, their low mechanical strength and poor controllability have been limitation for their usage as hard tissue regeneration. To overcome this limitation, α-tricalcium phosphate (α-TCP) has been used for biomedical scaffolds. α-TCP is biocompatible and soluble material, and hydrolyzed rapidly to calcium-deficient hydroxyapatite which makes α-TCP a useful material for bone tissue regeneration. in this study, we fabricate 3-dimensional (3D) scaffold based on α-TCP/collagen struts coated with collagen-based bioink. To compare the physical and cellular activities, we used a scaffold composed of α-TCP/collagen scaffold coated with cell-embedded collagen. Following fabrication of the cell (MC3T3-E1)-embedded a-TCP/collagen scaffold, the cellular activities were evaluated in vitro.
김예슬(YeSeul Kim),김민성(Minseong Kim),전호준(Hojun Jeon),김용복(YongBok Kim),이형진(Hyeongjin Lee),황헌(Heon Hwang),조재열(JaeYoul Cho),김근형(GeunHyung Kim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
The inherent properties of nanosized silica (sil), such as high biocompatibility, chemical and colloidal stability, and easy surface modification, have provided silica materials with a tremendous potential in biomedical applications. In this study, the biocomposites consisting of poly (ε-caprolactone) (PCL) and Sil fabricated by a melt-plotting/coating process can be applied as a potential scaffold for bone tissue regeneration. The pore size and strut diameter of the multi-layered biocomposites were fixed at approximately 300㎛ and 300㎛, respectively, and the morphology, hydrophilic properties, water-absorption, and mechanical strength of various compositions (1.8, 4.8, 9.4wt% of sil) in the composites were evaluated. Through the water-contact angle and water-absorption, the bio-composites displayed dramatically increased hydrophilic properties, and highly roughened surface compared to the pure PCL scaffold. The in vitro biocompatibilities (cell proliferation and mineralization) of the bio-composites wereexamined using pre-osteoblasts (MC3T3E1). Based on scanning electron microscope images, the cells were more easily adhered and grown on the surface of the bio-composites, showing enhanced mineral deposition compared to the pure PCL scaffold after 14 days of cell culture. These results were because the coated sil component in the bio-composites could induce the osteogensis of the composites. Based on the physical and biological activities, we believe that the biocomposite will be a potential biomaterial for enhancing bone tissue regeneration.
폴리카프로락톤/알지네이트로 구성된 바이오 용액코팅 공정의 하이브리드 세포담체 제작
김용복(YongBok Kim),이형진(Hyeongjin Lee),안승현(SeungHyun Ahn,),전호준(HoJun Jeon),여명구(MyungGu Yeo),이경호(KyoungHo Lee),김민성(Minseong Kim),김근형(GeunHyung Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
Alginate has been widely used in various tissue regenerations, and it has also recently been applied to bone tissue regeneration due to its ability to promote cell growth and bone tissue development. However, although alginate has excellent biocompatibility for the tissue regeneration, it suffers from low mechanical properties and low processability to obtain artificially controlled internal microstructures. In this study, to overcome the limitations, we propose a new cell-coating process supplemented with cells in alginate. The cell-coated PCL/alginate scaffold consisted of alginate with a mixture of cells coated on the PCL struts. The fabricated scaffold showed highly cellular behavior and uniform cell density.