도축폐수에서 분리된 Staphylococcus lentus에 의한 도축폐수의 성상 변화

손연주,박재림 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-

2000년 4월부터 7월까지 3차례예 걸쳐 채취한 도축폐수에서 세균을 분리, 동정하였다. 동정된 미생물의 차점종 S. lentus에 적합한 배양조건을 파악하고 도축폐수의 성상변화를 실험하여 아래와 같은 결과를 얻었다. S. lentus의 최적 배양조건은 nutrient broth에서 35℃, 120 rpm이였다. 도축폐수의 DO는4.14 mg/l, BOD는 1731.21 mg/l, BOD/COD_(cr),비는 0.53~0.64, T-P/T-N의 비는 1.00~1.41, 생균수는 5.47×10^(7) CFU로 나타났다. T-N의 변화는 배양 36시간까지 17% 감소에 그쳤고, NH^(4+)-N은 배양 36시간까지 모든 시료에서 증가하였고, nutrient broth에 도축폐수 원액 10%를 첨가한 시료에서 초기 29.19 mg/l에서 36시간에 570.36 mg/l으로 19.5배가 증가하였으나 원액 3%에서 초기 9.7 mg/l에서 403 mg/l로 41.6배의 가장 큰 효능을 나타냈다. NO_(3)^(-)-N은 nutrient broth에 원액 10%를 첨가한 시료에서 S. lentus에 의하여 배양초기에 73.31 mg/l에서 24시간까지 33.56 mg/l로 급격하게 감소되었다. T-P는 nutrient broth에 원액 10%를 첨가했을 때 S. lentus에 의하여 24시간동안 193.81 mg/l에서 101.41 mg/l까지 지속적으로 감소되었으며,5% 첨가는 배양 12시간 동안 188.74 mg/l에서92.59 mg/l로 급격하게 감소되었다 This study was carried out to check changes of components in the slaughter waste by the bacteria isolated from slaughter wastes from Gyungnam Province from May to June 2000, and to find useful organism for treatment of the waste. Bacteria used in this study were Staphylococcus lentus, as the second dominant of the waste. Optimum conditions for bacterial culture were obtained as the temperature of 35℃, pH 6.5, and shaking of 120 rpm in nutrient broth. The mean values of dissolved oxygen was 4.14 mg/l; biochemical oxygen demand, 1731.21 mg/l; ratio of BOD/COD, 0.53-0.64; ratio of T-P/T-N, 1.0-1.41; and viable counts of the waste, 5.47x10^(7) CFU. Little change in total nitrogen observed by 36 hr of the culture. The largest amount of increasing NH_(4)^(+)-N(19.5 times) was observed in the sample that 10% of the waste added in nutrient broth with S. lentus showing the value of 29.19 mg/l at the beginning to 570.36 mg/l by 36 hr of culture. However, the highest increasing ratio between initial amount and finals at 36 hr of culture showed as 41.6 times(from 9.7 to 403 mg/I) when 3% of the waste added. NO_(3)-N was decreased showing the value of 73.31 mg/I to 33.56 mg/I by 24 hr of culture with the organism when 10% of the waste added in nutrient broth. Total phosphorus was decreased showing the value from 188.74 mg/I to 92.59 mg/I after 12 hr of culture with the organism when 5% of the waste added in nutrient broth, while T-P was decreased gradually by 24 hr of culture from 193.8 to 101.4 mg/l when 10% of the waste added.

낙동강 하류(물금)와 세 지류에서 분리된 우점 세균의 증식에 미치는 금속(Cd & Zn) 및 그 질소 화합물의 영향

박재림,손연주,하경,박중철 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

1999년 5월부터 12월까지 한달 간격으로 부산·경남 인근의 주요 취수원인 물금취수장과 낙동강 중·하류에 위치한 지류 세 지점(남강, 황강, 금호강)에서 생균수와 우점종을 밝혀내고, 배양을 통해 우점종의 증식에 미치는 중금속의 영향을 실험하였다. 조사기간동안 지류별 생균수의 평균값(log_10)은 물금에서 가장 낮았고(3.4 CFU), 황강(3.709 CFU), 남강, 금호강 순으로 나타났다. 특히, 금호강 지점은 다른 지점보다 월등하게 높은 값을 보여주었다. 전 지점에서 Staphylococcus가 가장 우점하는 것으로 나타났으며, P. pneumotropica, S. lentus, Micrococcus spp.가 차점종으로 나타났다. 전 조사 지점의 공통 우점종을 배양하면서 이들의 증식에 미치는 중금속의 영향을 실험한 결과 남강에서 분리된 Micrococcus spp.는 Cd와 Zn분말 및 질소화합물에 대해 전반적으로 증식이 억제되는 것으로 나타났다. P. pneumotropica는 Cd과 Zn분말 첨가했을 때 증식억제가 뚜렷하게 일어났으나, 각각의 질소 화합물에 대해서는 증식이 대조군과 유사하거나, 보다 낮은 증식억제력을 나타내었다. A. hydrophilla도 Cd과 Zn분말, Zn(NO_3)_26H_2O에 대해 증식이 크게 억제되었으나, Cd(NO_3)_24H_2O 첨가시 대조군 증식 곡선에 비해 증식력이 ⅓~⅔에 불과했다. S. lentus는 분리된 시기마다 증식억제력에 차이를 보였으나, Cd과 Zn분말 그리고 그 화합물에 대해 증식억제력이 대체로 낮은 것으로 나타났다. Investigation was carried out to observe the dominant bacteria and the effect of metals(Cd & Zn) and its nitrate compound on growth of bacteria isolated from the three tributaries and lower reach of the Nakdong River. Mean CFU(log_10) level was highest in Kumho River(8.30 CFU), Nam River, Hwang River, and Mulgum followed. Staphylococcus xylosus, Staph. lentus, Pasteurella pneumotropica, Aeromonas hydrophilla were dominant species in each study site. Cadmium powder and Zinc powder showed strong effect to inhibit the growth of Micrococcus spp., Pasteurella pneumotropica, Aeromonas hydrophilla. But, nitrate compoundes of Cd and Zn(Cd(NO_3)_24H_2O, Zn(NO_3)_26H_2O) did not clearly show the strong effect to inhibit the growth of dominants.

심포지움 : Chitosan Nanoaggregates for Tumor Targeting Drug Delivery System Using EPR Effect

(Yoen Ju Son),(Yong Woo Cho),(Hesson Chung),(Seo Young Jeong),(Chong Rae Park),(In San Kim),(Sang Bong Seo),(Ick Chan Kwon) 한국키틴키토산학회 2002 한국키틴키토산학회지 Vol.7 No.1

Transdermal Delivery of Hydrocortisone Using Self-Emulsifying Nanoemulsion System

Kang Heui-Il,Park Young-Joon,Son Yoen Ju 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.1

The Symptoms of Atopic Dermatitis in NC/Nga Mice Were Significantly Relieved by the Water Extract of Liriope platyphylla

Ji Eun Kim,Yoen Kyung Lee,So Hee Nam,Sun Il Choi,Jun Seo Goo,Min Ju Jang,Hee Seob Lee,Hong Ju Son,Chung Yeol Lee,Dae Youn Hwang 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.4

Atopic dermatitis is an well-known skin disease showing inflammatory, chronically relapsing, noncontagious and pruritic symptoms. The aims of this study were to investigate the effects of Liriope platyphylla (LP) on atopic dermatitis of NC/Nga mice. To achieve this, NC/Nga mice were treated with four different conditions including vehicle, phthalic anhydride (PA), PA+5% LP and PA+10% LP, and the changes of immune-related factors were detected after 2 weeks. The pathological phenotypes of atopic dermatitis such as erythema, ear thickness, edema, scab and discharge were significantly decreased in PA+10% LP cotreated groups compare to PA treated group. Also, the weight of lymph node and thymus in immune organs were gradually decreased in LP treated groups, while the weight of spleen was slightly increased in same group. Furthermore, toluidine blue staining analysis, a method used to specifically identify the mast cell, showed that the decrease of master cell infiltration into the dermis were statistically observed in PA+5% LP and PA+10% LP5% cotreated groups. Especially, the decrease of IgE concentration was detected only PA+10% LP cotreated group, although this level was maintained in PA+5% LP cotreated group. Therefore, these results suggested that the water extracts of LP may contribute the relieve of atopic dermatitis symptoms and be considered as an excellent candidate for a atopic dermatitis-therapeutic drug.

The Symptoms of Atopic Dermatitis in NC/Nga Mice Were Significantly Relieved by the Water Extract of Liriope Platyphylla

Ji Eun Kim,Yoen Kyung Lee,So Hee Nam,Sun Il Choi,Jun Seo Goo,Min Ju Jang,Hee Sup Lee,Hong Ju Son,Chung Yeol Lee,Dae Youn Hwang 한국실험동물학회 2011 한국실험동물학회 학술발표대회 논문집 Vol.2011 No.2

Topical Application of Selenium Can Significantly Relieve UV-induced Skin Aging in Hairless Mice

So Hee Nam,Seung Eun Jung,Yoen Kyung Lee,Ji Eun Kim,Eon Pil Lee,Hae Wook Choi,Hong Sung Kim,Jae Ho Lee,Young Jin Jung,Chung Yeol Lee,Hong Ju Son,Hyun Woong Lee,Jung Sik Cho,Byeong-Cheol Kang,Dae Youn 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.1

Ultraviolet (UV) irradiation is an environmental factor that causes skin aging, and is also a major factor leading to cumulative alterations of skin structure, function and appearance. To investigate the effects of Selenium (Sel) on UV-induced skin aging, hairless mice were treated for 4 weeks with UV irradiation and topical application of Sel. Then, the effects of Sel were measured in the skin of these mice via histological analysis and Western blotting. According to the results of wrinkle formation analysis, the topical application of Sel induced a reduction in wrinkling formation in the damaged skin of the UV-irradiated mice. Additionally, our histological analysis demonstrated that the skin thickness in the Sel-treated group was less than in the UV-irradiated group. Furthermore, in an effort to investigate the mechanisms underlying the effects of Sel, the expression levels of matrix-metalloproteinase (MMP) and MAPK protein were assessed in both groups. The application of Sel induced a reduction in MMP-1 expression levels to the levels observed in the non-irradiated group. However, the expression level of MMP-9 was increased slightly in the Sel application group as compared with the vehicle application group. Additionally, the levels of ERK phosphorylation were increased by the application of Sel, but the levels of JNK and p38 were not altered by Sel treatment. These results suggest the possibility that Sel should be considered as a skin aging-protective and therapeutic drug candidate, which functions via the regulation of MMP expression levels.