질적지표를 활용한 국내 지적장애 대상 비디오 모델링 중재특성 분석

안예지 ( An¸ Yeji ),이미지 ( Lee¸ Miji ) 한국지적장애교육학회(구 한국정신지체아교육학회) 2020 지적장애연구 Vol.22 No.4

연구목적: 본 연구는 국내 지적장애 대상 비디오 모델링 중재연구를 종합하여 그 특성을 파악하고, 질적지표에 근거한 분석을 통해 증거기반실제의 기초를 마련함으로써 향후 연구와 실제를 위한 시사점을 얻는 것을 목적으로 한다. 연구 방법: 이를 위해 2000년도 이후 국내 학술지에 발표된 논문 중, 선정기준을 충족시키는 연구 총 21편을 선정하고 질적지표를 활용하여 중재특성을 분석하였다. 연구 결과: 2000년 이후 지적장애인을 대상으로 한 비디오 모델링 중재연구는 2006년과 2015년 사이에 가장 많이 이루어졌으며, 연구의 형태는 단일대상 연구의 비율이 높게 나타났다. 비디오 모델링 중재는 초등학교 급에서 일상생활기술 증진을 위해 단독중재형태로 학교에서 가장 많이 이루어졌다. (준)집단설계연구의 질적지표 분석 결과, 3편 중 1편만이 충분한 질적 수준을 보인 반면, 단일대상연구 18편 중 17편이 평균 2.0점 이상으로 질적지표 충족 수준이 상대적으로 높게 분석되었다. 결론: 본 연구의 결과를 바탕으로 지적장애 대상 비디오 모델링 중재연구의 질적 수준을 확보하고 효과적인 증거기반 중재 제공을 위한 함의를 논하였다. Purpose: This study aims to synthesize video modeling(VM) intervention studies using quality indicators to examine effects of VM for individuals with intellectual disabilities. Method: To this end, a total of 21 Korean articles published between 2000 and 2020 were selected for analysis. Results: (a) about 70% of VM intervention studies for people with intellectual disabilities were conducted between 2006 and 2015; (b) majority of studies were practiced in single subject design; (c) VM interventions were often provided in school settings to improve independent living skills at the primary school level; (d) findings from the analysis of quality indicators also concluded that only 1 out of 3 quasi-group-design studies showed sufficient level, whereas 17 out of 18 single-subject-design studies showed a relatively high level of satisfaction with an average of 2.0 points or higher. Conclusion: Based on the results, implications for securing the quality level of VM intervention studies for people with intellectual disabilities and providing effective evidence-based interventions were discussed.

조리 방법에 따른 고구마줄기의 Phytochemical 함량과 항산화 활성 비교

양세희, 정혜진, 성예지, 이하나, 이세윤, 양진우, 이준수 忠北大學校 農業科學硏究所 2017 農業科學硏究 Vol.33 No.1

Vegetable consumption is associated with health benefits. Recently, the interest in these food groups has grown among due to the identification of new compound in their chemical composition. Most of the vegetables are cooked by blanching, boiling, steaming, microwaving before consumed. These cooking processes may change not only food physical characteristics but also chemical composition. The present study was undertaken to investigate the effect of different cooking methods on phytochemical content and antioxidant capacity in Ipomoea batatas (L.) Lam stem. The result of study, Total polyphenol of phytochemical in Ipomoea batatas (L.) Lam stem was 6.88-12.65 mg GAE/100 g and flavonoid was 3.16-10.46 mg CE/100 g. Compared with the raw of Ipomoea batatas (L.) Lam stem, all cooking methods were found to significant correlations between the antioxidant activity assessed by ABTS, DPPH and polyphenol(p<0.05). These results can be provide the basic data how to prevent lose of nutrition in Ipomoea batatas (L.) Lam stem when cooking.

Transient exposure to hydrogen peroxide inhibits the ubiquitination of phosphorylated IκBα in TNFα-stimulated HEK293 cells

Yeji Lee,Jin Choi,Kyung-Ho Ha,주대명 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.8

During ischemia-reperfusion injury, brief pre-exposure to oxidative stress renders organs resistant to subsequent severe damage. NF-κB is a transcription factor that is involved in reperfusion-induced inflammatory and immune responses. The activity of NF-κB has been shown to be modulated by oxidative stress in various cell types through different pathways. We studied the effect of pre-exposure to oxidative stress on subsequent NF-κB activation in TNFα-stimulated HEK293 cells. The cells were transiently exposed to 0.5 mM H2O2 for 20 min, prior to stimulation with TNFα, and the subsequent expression of NF-κB-dependent genes and the levels of NF-κB signaling molecules were measured. Pre-exposure to H2O2 significantly delayed the TNFα-induced expression of an NF-κB reporter gene and inflammatory proteins (intercellular adhesion molecule-1 and IL-1β). The degradation of inhibitor of NF-κB α (IκBα) and the nuclear translocation of NF-κB were also delayed by H2O2 treatment,whereas IκBα phosphorylation and IκB kinase activity were not changed. When we examined the ubiquitin/proteosome pathway in H2O2-treated cells,we could not detect significant changes in proteosomal peptidase activities, but we were able to detect a delay of IκBα poly-ubiquitination. Our results suggest that transient exposure to oxidative stress temporally inhibits NF-κB-dependent gene expression by suppressing the poly-ubiquitination of phosphorylated IκBα in HEK293 cells.

Microbiota-derived lactate promotes hematopoiesis and erythropoiesis by inducing stem cell factor production from leptin receptor+ niche cells

Lee Yong-Soo,Kim Tae-Young,Kim Yeji,Kim Seungil,Lee Su-Hyun,서상욱,Zhou Bo O.,Eunju O.,김광순,권미나 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Although functional interplay between intestinal microbiota and distant sites beyond the gut has been identified, the influence of microbiota-derived metabolites on hematopoietic stem cells (HSCs) remains unclear. This study investigated the role of microbiota-derived lactate in hematopoiesis using mice deficient in G-protein-coupled receptor (Gpr) 81 (Gpr81 − /− ), an established lactate receptor. We detected significant depletion of total HSCs in the bone marrow (BM) of Gpr81 −/− mice compared with heterogenic (Gpr81 +/− ) mice in a steady state. Notably, the expression levels of stem cell factor (SCF), which is required for the proliferation of HSCs, decreased significantly in leptin receptor-expressing (LepR + ) mesenchymal stromal cells (MSCs) around the sinusoidal vessels of the BM from Gpr81 −/− mice compared with Gpr81 +/− mice. Hematopoietic recovery and activation of BM niche cells after irradiation or busulfan treatment also required Gpr81 signals. Oral administration of lactic acid-producing bacteria (LAB) activated SCF secretion from LepR + BM MSCs and subsequently accelerated hematopoiesis and erythropoiesis. Most importantly, LAB feeding accelerated the self-renewal of HSCs in germ-free mice. These results suggest that microbiota-derived lactate stimulates SCF secretion by LepR + BM MSCs and subsequently activates hematopoiesis and erythropoiesis in a Gpr81-dependent manner.

Factors Related to Smoking Status Among Young Adults: An Analysis of Younger and Older Young Adults in Korea

Lee, Yeji,Lee, Kang-Sook The Korean Society for Preventive Medicine 2019 Journal of Preventive Medicine and Public Health Vol.52 No.2

Objectives: Young adulthood represents a critical developmental period during which the use of tobacco may begin or cease. Furthermore, differences in smoking behaviors between younger (aged 18-24 years) and older (aged 25-34 years) young adults may exist. This study aimed to characterize patterns related to current smoking in younger and older young adults. Methods: This study used data acquired from the Sixth Korea National Health and Nutrition Examination Survey conducted from 2013 to 2014. A total of 2069 subjects were categorized as younger (712 subjects) and older (1357 subjects) young adults. The chi-square test was used to assess the relationships between smoking status and socio-demographic, health-related, and smoking-related factors. Multivariable logistic regression models were constructed to assess the factors affecting current smoking in these age groups. Results: The current smoking prevalence was 18.3% among the younger young adults and 26.0% among the older young adults. Sex, education level, occupation, perceived health status, alcohol consumption, and electronic cigarette use were related to current smoking in both age groups. Secondhand smoke exposure at home and stress levels showed significant relationships with smoking in younger and older young adults, respectively. Conclusions: Strong correlations were found between the observed variables and smoking behaviors among young adults. Determining the factors affecting smoking and designing interventions based on these factors are essential for smoking cessation in young adults.

Immunogenicity of lipid nanoparticles and its impact on the efficacy of mRNA vaccines and therapeutics

Lee Yeji,Jeong Michaela,Park Jeongeun,Jung Hyein,Lee Hyukjin 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Several studies have utilized a lipid nanoparticle delivery system to enhance the effectiveness of mRNA therapeutics and vaccines. However, these nanoparticles are recognized as foreign materials by the body and stimulate innate immunity, which in turn impacts adaptive immunity. Therefore, it is crucial to understand the specific type of innate immune response triggered by lipid nanoparticles. This article provides an overview of the immunological response in the body, explores how lipid nanoparticles activate the innate immune system, and examines the adverse effects and immunogenicity-related development pathways associated with these nanoparticles. Finally, we highlight and explore strategies for regulating the immunogenicity of lipid nanoparticles.

Morus alba L. Root Reduced MIFT Expression by Inhibiting Cell Melanin Accumulation in B16F10 Cells

Yeji Lee,So-Yeon Lee,Yulong Zheng,Il-Jun Kang 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Transient exposure to hydrogen peroxide inhibits the ubiquitination of phosphorylated IκBα in TNFα-stimulated HEK293 cells

Lee, Yeji,Choi, Jin,Ha, Kyung-Ho,Jue, Dae-Myung Korean Society for Biochemistry and Molecular Biol 2012 Experimental and molecular medicine Vol.44 No.8

<P>During ischemia-reperfusion injury, brief pre-exposure to oxidative stress renders organs resistant to subsequent severe damage. NF-κB is a transcription factor that is involved in reperfusion-induced inflammatory and immune responses. The activity of NF-κB has been shown to be modulated by oxidative stress in various cell types through different pathways. We studied the effect of pre-exposure to oxidative stress on subsequent NF-κB activation in TNFα-stimulated HEK293 cells. The cells were transiently exposed to 0.5 mM H<SUB>2</SUB>O<SUB>2</SUB> for 20 min, prior to stimulation with TNFα, and the subsequent expression of NF-κB-dependent genes and the levels of NF-κB signaling molecules were measured. Pre-exposure to H<SUB>2</SUB>O<SUB>2</SUB> significantly delayed the TNFα-induced expression of an NF-κB reporter gene and inflammatory proteins (intercellular adhesion molecule-1 and IL-1β). The degradation of inhibitor of NF-κB α (IκBα) and the nuclear translocation of NF-κB were also delayed by H<SUB>2</SUB>O<SUB>2</SUB> treatment, whereas IκBα phosphorylation and IκB kinase activity were not changed. When we examined the ubiquitin/proteosome pathway in H<SUB>2</SUB>O<SUB>2</SUB>-treated cells, we could not detect significant changes in proteosomal peptidase activities, but we were able to detect a delay of IκBα poly-ubiquitination. Our results suggest that transient exposure to oxidative stress temporally inhibits NF-κB-dependent gene expression by suppressing the poly-ubiquitination of phosphorylated IκBα in HEK293 cells.</P>

Hydrogen production from the photocatalytic hydrolysis of sodium borohydride in the presence of In–, Sn–, and Sb–TiO₂s

Lee, Yeji,Kim, Youngmi,Jeong, Harim,Kang, Misook Elsevier 2008 Journal of industrial and engineering chemistry Vol.14 No.5

<P><B>Abstract</B></P><P>To enhance the evolution of hydrogen from the photocatalytic hydrolysis of sodium borohydride solution, three photocatalysts; 0.5mol% In, Sn, and Sb, incorporated into TiO<SUB>2</SUB>, were prepared using a hydrothermal method. The particle sizes of well-developed anatase were about 5.0nm, except those for Sb–TiO<SUB>2</SUB>, which were above 5.0nm, with closed to cubic form shapes, except for pure TiO<SUB>2</SUB> that was spherical. In the X-ray photoelectron spectra (XPS) for Ti2p<SUB>3/2</SUB>, a band at 460.34eV, attributed to partial MTiO<SUB>3</SUB>, was seen in Sn–TiO<SUB>2</SUB>. Additionally, two bands of Sn3d<SUB>5/2</SUB> at 484.5 and 486.8eV, assigned to the Sn and SnO or SnO<SUB>2</SUB>, respectively, appeared over Sn–TiO<SUB>2</SUB>, with the ratios of metal–OH/metal–O in the O1s bands, imply a hydrophilic property, also increased in this compared with the other catalysts. The hydrogen evolution from sodium borohydride (NaBH<SUB>4</SUB>) hydrolysis was enhanced in the Sn–TiO<SUB>2</SUB> photocatalytic system compared to those for the other catalysts; hydrogen was evolved at 140ml/gh over Sn–TiO<SUB>2</SUB>, which was different from the 100ml/gh for the hydrolysis of pure NaBH<SUB>4</SUB>, and increased to 1350ml/g after 5h. In particular, the evolved hydrogen increased with increasing amounts of Sn–TiO<SUB>2</SUB>, and the production continued for 150h.</P>

Rapid and real-time diagnosis of hypoalbuminemia using an extraordinary optical transmission biosensor

Lee, Yeji,Song, Hyerin,Ahn, Heesang,Choi, Jong-ryul,Kim, Kyujung Elsevier 2018 Sensors and actuators. B Chemical Vol.274 No.-

<P><B>Abstract</B></P> <P>A hypoalbuminemia diagnosis kit, based on an extraordinary optical transmission sensor, for accurate and fast measurements of albumin concentrations in human serum was demonstrated. First, using nanohole arrays, the sensitivity of the sensor was confirmed to be approximately 460 nm/RIU by theoretical simulations and using standard solutions with specific refractive indices. In the preliminary study of albumin measurements, non-labeled detection of a difference in albumin concentration of approximately 10 mg/ml was achieved with a spectral peak shift of 0.8972 nm and proved to be capable of diagnosing hypoalbuminemia without a surface modification process. Additionally, we successfully classified 100 samples with different concentrations into normal and hypoalbuminemia groups with a probability greater than 98%. According to the results, the hypoalbuminemia diagnosis kit has a potential to be practically employed for real-time, preclinical, label-free diagnosis with integration of advanced microfluidics and miniaturized optics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A hypoalbuminemia diagnosis kit based on an EOT sensor was demonstrated. </LI> <LI> The sensitivity of the sensor with nanohole arrays is 463.6 nm/RIU. </LI> <LI> Label free detections for the difference of albumin concentrations about 10 mg/ml was achieved. </LI> <LI> Normal and hypoalbuminemia groups was distinguished with a probability of over 98%. </LI> </UL> </P>