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Isolation and Transcriptional Expression of CuZn Superoxide Dismutase from Codonopsis lanceolata
Lee,Kang,In,Jun-Gyo,Yu,Chang-Yeon,Yun,Song-Joong,Min,Byung-Hoon,Rho,Yeong-Deok,Kim,Moo-Sung,Yang,Deok-Chun 한국자원식물학회 2004 Plant Resources Vol.7 No.3
To investigate the defense mechanism against the abiotic stress, a cDNA clone encoding a CuZn superoxide dismutase (CuZnSOD) protein was isolated from a cDNA library prepared from tabroot mRNAs of Codonopsis lanceolata. The eDNA, designated ClSODCc, is 799 nucleotides long and has an open reading frame of 459 bp with a deduced amino acid sequence of 152 residues. The deduced amino acid sequence of ClSODCc matched to the previously reported CuZnSODs. Consensus amino acid residues (His-45, -47, -62, -70, -79, -119 and Asp-82) were involved in Cu-, Cu/Zn-, and Zn- binding ligands. The deduced amino acid sequence of ClSODCc showed high homologies (82%-86%) regardless of species. Expression of ClSODCc by oxidative stress was increased up to 1 h after treatment and declined gradually. Much earlier and stronger expression of ClSODCc was observed in the cold stress treatment.
Agrobacterium tumefaciens C58에 依하여 形質轉換된 人蔘組織에서의 saponin 生産
梁德春,李政明 경희대학교 부설 식량자원개발연구소 1988 硏究論文集 Vol.9 No.-
The present study was conducted to obtain some basic information on the transformation of ginseng tissue, identification of opine compound and protein, and saponin production from ginseng tissue transformed with pTiC58. Ginseng tumor callus induced by pTiC58 could be continuously cultured on the phytohormone-free basic medium. The transformation was reconfirmed by the detection and identification of opine compounds from the tumor callus. The transformed ginseng calli contained higher amount of protein than normal calli and the protein pattern of transformed calli was quite different from that of normal callus. Higher saponin content was found in the transformed callus than the normal callus, and ginsenoside composition of the transformed callus was similar to that of cultivated ginseng root, but quite different from that of normal callus.
李允洙,全洋德 三陟大學校 1992 論文集 Vol.25 No.-
Beginning with the industrial revolution in England, spreaded rapidly into European countries, the design movement, new artistic concept, faced with a new phase because of more developed industrial technique and international age. Identity concept in design, with industrial diversity, specialization, internationalization, resulted from the work called 'Visual Identification Standards.' Samchok National University, elevated into four-years' academic system in 1991, has taken a new opportunity of making progress. Keeping pace with the new image, the introduction to Visual Identification Standard is followed necessarily according to the current trend. The V.I.S. Manual has been attempted by general concept. Some problems on the overall plan, design process, and management in the Manual are as follows; 1. It has not gone through the broad convergence of opinions. That is, the identity n ideal system has not ben established. But the Visual Standard of identity was committed. 2. The Visual Identification Standard has been published into a Manual. But it has been used missunderstandingly, or subjectivelly. 3. For the unified image, it should be widely used, and the visual unfamiliarization should be eliminated. Thus, V.I.S. Manual of Samchok National University should be sufficed gradually according to the various phases. In a broad view, the re-design concept should be examined and thoroughly.
Ti-plasmid에 依하여 形質轉換된 煙草組織의 特性과 再分化 樣相
梁德春,李政明 경희대학교 부설 식량자원개발연구소 1987 硏究論文集 Vol.8 No.-
Studies were conducted to examine the characteristics of teratoma shoots obtained from diploid and haploid tobacco crown gall tumor tissues induced by the inoculation of Agrobacterium tumefaciens and the effects of plant hormones and active carbon in the cultural media on the growth and regeneration patterns of the teratoma shoots. Results can be summarized as follows. 1. Teratoma shoots could be obtained when the crown gall tumor, induced by the inoculation of A. tumefuciens strain C-58 to the stem, was successively grown on hormone-free medium. 2. The growth of tumor callus induced by C-58 strain was inhibited when several plant hormones were added to the cultural media. Plant regeneration could not be obtained from the tumor callus or the teratomas. 3. Addition of active carbon and benzyl adenine to cultural media was effective for shoot elongation and reduction in multiple shoot formation. 4. The growth of tumor callus obtained from the haploid plant was promoted by the addition of plant hormones to the media. Whole plants were also regenerated from the teratoma shoots formed from tumor callus. 5. Normal or transformed plants were regenerated from the tumor callus formed by the inoculation of a strain of A. tumefaciens collected from field soil. The transformed plants are characterized by having twisted dark-green leaves, shorter internodes, and abundant axillary shoots.
만성폐쇄성폐질환의 치료에서 서방형 Theophylline 의 1 일 1 회 제형과 1 일 2 회 제형의 비교
이양덕(Yang Deok Lee),이흥범(Heung Bum Lee),이용철(Yong Chul Lee),이양근(Yang Keun Rhee) 대한내과학회 2002 대한내과학회지 Vol.62 No.2
N/A Background: Theophylline is a weak bronchodilator with a narrow therapeutic window. Much of its clinical benefit derives from effects other than bronchodilaton; therapeutic doses of theophylline increase ventilatory drive, enhance diaphragmatic contractility and increase cardiac output. About 20% of COPD patients respond to theophylline with improved airflow, exercise tolerance and quality of life. Generally it is prescribed as a twice-daily equal-dose regimen so as to produce nearly constant blood level throughout each 24 hour. We compared 12-hour and 24-hour sustained-release theophyllines in the management of chronic obstructive pulmonary disease. Methods: A crossover study was conducted with 27 adult chronic obstructive pulmonary disease requiring daily bronchodilator therapy. The study group included nineteen males and eight females ranging in age from 48 to 71 years. The overall approach was to place the patients first on the twice-daily preparation (Etheophyl?) for 28 days at 8 AM and 8 PM and pulmonary function tests and theophylline level were measured on the 28th day. The patients were subsequently switched to the once-daily preparation (Uniphyl?) in the same daily dose at 8 PM on the 29th day and the same parameters were measured on the 56th day. Results : The mean serum levels of theophylline were 8.80±2.64 μg/mL in the Etheophyl?- treated and 8.48±2.08 μg/mL in the Uniphyl?-treated period. And the FEV1 showed 63.72±8.63% in the Etheophyl?-treated and 62.81±9.81% in the Uniphyl?-treated period. Conclusion: The results suggested that there was little clinical differences between the two medications. The two drugs were equally effective in clinically controlling COPD over the four weeks of treatment.(Korean J Med 62:182-186, 2002)
Yang, Seung-Hwan,Lee, Chun-Gu,Lee, Won-Kyu,Ashtiani, Alireza Araghi,Kim, Joon-Yong,Lee, Sang-Deok,Rhee, Joong-Yong Korean Society for Agricultural Machinery 2012 바이오시스템공학 Vol.37 No.1
Purpose: Utilizing air thermal energy during over-heated time in the greenhouse is a necessary component to save greenhouse heating costs for nighttime. However, there is no practical way to implement the related principles. Methods: In this study, a heating and cooling system which utilizes the surplus air thermal energy in a greenhouse was developed. Available air thermal energy and heating load for this experimental glasshouse were estimated based on temperature conditions of the plant growth and weather data. Results: Estimated values were 400 MJ/day for maximum surplus air thermal energy and 340 MJ/day for maximum heating energy which were target values of the design as well. The system consists of a heat pump, fan-coil units and heat storage tanks which are divided into low and high temperature tanks. Moreover, a new control logic was developed for surplus air thermal energy utilization. Conclusions: This paper explains the details of conceptual design process of the system. Results of test operations showed that the developed system performed the recovery and supply of the thermal energy according to design purposes.
Expression Profiling of Calcium Induced Genes in Cultured Human Keratinocytes
Lee, Jung-Suk,Kim, Mi-Rang,Kim, Nam-Soon,Kim, Yong Sung,Yang, Jun-Mo,Cho, Ah Young,Lee, Young,Kim, Chang Deok,Lee, Jeung-Hoon The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.4
<P>Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.</P>