Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang, Chul Hak,Jun, Gyo,Lee, Dug Keun,Lee, Sang Kyou,Park, Se Yeon,Hahm, Eun Ryeong 생화학분자생물학회 1998 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the conventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Yang,Chul-Hak,Jun,Gyo,Hahm,Eun-Ryeong,Lee,Sangkyou,Park,Seyeon,Lee,Dug-Keun The Korea Science and Technology Center 1999 BMB Reports Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 uncleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

Quantitative Assay for the Binding of Jun-Fos Dimer and Activator Protein-1 Site

Lee, Sang-Kyou,Park, Se-Yeon,Jun, Gyo,Hahm, Eun-Ryeong,Lee, Dug-Keun,Yang, Chul-Hak Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.6

The Jun and Fos families of eukaryotic transcription factors form heterodimers capable of binding to their cognate DNA enhancer elements. We are interested in searching for inhibitors or antagonists of the binding of the Jun-Fos heterodimer to the activator protein-1 (AP-1) site. The basic-region leucine zipper (bZIP) domain of c-Fos was expressed as a fusion protein with glutathione S-transferase, and allowed to form a heterodimer with the bZIP domain of c-Jun. The heterodimer was bound to glutathione-agarose, to which were added radiolabeled AP-1 nucleotides. After thorough washing, the gel-bound radioactivity was counted. The assay is faster than the coventional electrophoretic mobility shift assay because the gel electrophoresis step and the autoradiography step are eliminated. Moreover, the assay is very sensitive, allowing the detection of picomolar quantities of nucleotides, and is not affected by up to 50% dimethylsulfoxide, a solvent for hydrophobic inhibitors. Curcumin and dihydroguaiaretic acid, recently known inhibitors of Jun-Fos-DNA complex formation, were applied to this Jun-GST-fused Fos system and revealed to decrease the dimer-DNA binding.

原價管理시스템을 利用한 綜合病院의 經營戰略

秋敎完,李永準 昌原大學校 産業經濟硏究所 1992 産經硏究 Vol.9 No.-

교복자율화가 성격특성에 미치는 영향

정준교,이선경 한국의류학회 1999 한국의류학회지 Vol.23 No.3

This study examined the effect of abolition of school uniform on personality. The subjects were 317 boys(mean age: 18) and 312 girls(mean age: 18) in third grade of high school. The subjects were selected from 4 uniform high schools and 4 free-choice wearing clothes high school, in which were situated at north and south part of Han-River in Seoul. They were administered with the following questionnaires to measure the creativity, individuality, self-efficacy, conformity of behaviour, conformity of attitude, autonomy and responsibility. Results indicated that (1) in many personality variables, there were no effect of abolition of school uniform on personality, (2) the effect of abolition of school uniform on personality must be considered in the moderator effect of the preferred clothes of students. These results were discussed and its implication and following study were suggested.

19세기 후반기 미국 노동(조합)운동의 재조명 : 노동과정(통제)및 여타 사회운동과의 관련에서 Labor Process & other Social Labor Movements

정준교 연세대학교 대학원 1987 원우론집 Vol.15 No.1

In this paper, I attempted to review American Labor(Union) Movement, in relation to Labor Process(control) theories and other Social Movements, in order to offer a frame of reference to understand the characteristics and change of Korean Labor(Union) Movement. Results of the study are as follows: Industrial Revolution in America began the late 18th century; however, America was industrialized with increasing speed after Civil War, Especially after the factory system was current around 1850, the Real Subsumption of labor to capital became universal. Industrialization led to monopoly of Capital and relative degradation of Labor So the laborers resisted against the industrialization and monopoly capital to recover their rights and interests and improve labor conditions with peasants, soialists, and middle classes seperatedly or in union The Union was typical and useful mechanism in the Labor Movement. Nation-wide unions were established around 1850 and National Unions such as National Labor union, National Colored Union, Knights of Labor, American Federation of Labor after Civil War as well. The relation of the labor process control strategies of capital to labor moveent was dialectical. First labor(union) movement was begun to struggle to the suppression of the capital. And capital used labor process control strategies such as craft control, divide & rule, simple or technical control before c 1870, technical or institutional control after that with other tactics to overturn the laborer's resistance. The organized laborer began struggle for autonomy guarantee, claim for humanitarion treatments through ethical code, and other countercontrols -expansion of union membership to, improvement of the working conditions of, company with unorganized laborers, or intensification of union's exclusiveness- , in respond to labor process control strategies. And again capital had responded with more elaborated strategy like the internal contracting system, or attempted to breakdown the Union like k.s of Labor. It is said that the characteristics of American Labor Movement has been the lack of class consciousness, business unionism(Pragmatism), etc.. But the results of this paper in relation to Marxian Labor Movement theories are as follows: 1) From the beginning, American Labor(Union) Movement attempted to take-off from the 3rd stage (formation of union and collective bargaining) to the 4th stage(political struggle). 2) But every trial of it did not succeeded for the weakness of union against crisis, the division & struggle with unions, the lack of opportunity cost concept of economic struggle, the lack of certain consiousness of marxian historical development. 3) The lack of class consiousness of labor union movement was not so much in false consiousness as in the ordeal state of consciousness then. 4) Knights of Labor got the concept of Labor Movement that the union movement must be with all laborers. This study have some limitations, such as 1) Lack of holistic recognition about soial formation and its transformation, Labor Process & Labor Movement, etc. 2) Failure of systematic review of State, middle classes, and other social movements.

고등학생의 학교몰입 증가와 중퇴의도 감소를 위한 교사의 지도성유형 검증

정준교 한국청소년복지학회 2001 청소년복지연구 Vol.3 No.1

This study investigates the teacher's leadership which could be increased the level of organizational commitment and decreased the level of intent to exit. The subjects were 239 boys (mean age: 17.83) and 294 girls (mean age: 17.94) in second grade of 4 academic high schools and 4 vocational high schools. Confirmatory factor analysis with LISREL 8 were used. Results indicate that (1) the type of teacher's leadership which could be increased the level of affective commitment AC) and decreased the level of continuance commitment (CC) are transformational leadership (charisma p<.05), (2) the type of teacher's leadership could be decreased EXIT are transactional leadership (p<0.05), (3) there is a positive relationship between CC and EXIT (p<.05). These results were discussed and its implication and following studies were suggested.

창의성, 개성, 동조성에 따른 고교생의 의복행동 연구

이선경,정준교 대한가정학회 2003 Family and Environment Research Vol.41 No.9

This study examined the clothing behavior in terms of creativity, individuality and conformity. The subjects were 317 boys(mean age: 18) and 312 girls(mean age: 18) in third grade of high school, in which were situated at north and south part of Han-River in Seoul. They were administered with the following questionnaires to measure the attitudinal and behavioral conformity, creativity, individuality, clothing conformity, clothing nonconformity, clothing confidence, aesthetic sense, clothing importance, fashionabdity. Results indicated that (1) high school students who have high creativity showed higher scores in clothing nonconformity, clothing confidence, aesthetic sew, clothing importance than those who have low creativity. But there were no significant differences between two groups in clothing conformity and fashionability(p<0.001). (2) High school students who have high individuality showed higher scores in clothing conformity clothing, nonconformity, clothing confidence, aesthetic sense, clothing importance and fashionability than those who have low individuality(p<0.001). (3) Students who have low attitudinal conformity showed higher scores in clothing nonconformity, clothing confidence and aesthetic sense(p<0.001) and lower score in information and norm specific clothing confirmity(p<0.05) than those who have high attitudinal conformity. And those who have high behavioral conformity showed higher score in clothing confirmity than those who have low behavioral confimity(p<0.05). From these findings, we could find that the importance of creativity and individuality was greater than that of conformity in clothing behavior. These results were discussed and following studies were suggested.

알레르기성 비염 환자의 기인 항원에 관한 고찰

고영한,박시영,이종환,구교준,구수권,이상화,김성원 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.1

Antidiabetic Effects of Wild Ginseng Cultured Root Extract in db/db mice

Jun-Gyo In,Deok-Chun Yang,Dae-Ik Kim,Ae-Kyung Lim,Young-Chul Kim,Mi-Ree Lee,Sung-Il Park,Dae-Whan Kim,Jae-Chan Song,Kil-Soo Kim 한국실험동물학회 2010 한국실험동물학회 학술발표대회 논문집 Vol.2010 No.8