담도폐쇄증 환아의 내인성 착색치아에 대한 증례보고

이창희,이상대,김영재,김정욱,한세현,이상훈 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.3

담도페쇄증은 간장외 담관의 부분적이거나 완전한 파괴 또는 부재로 인한 담즙흐름의 완전한 폐쇄로 정의된다. 이 질환은대략 10,000명당 1명 정도 비율로 발생하며 여성에서 약간 높은 빈도를 보인다. 원인인자는 명확히 밝혀지지 않았으며 증상은 과빌리루빈혈증과 함께 황달, 점토색변, 지방설사, 어두운 갈색뇨, 간비대 등을 보인다. 최근의 담도폐쇄증의 치료는 간문장문합술 단독 혹은 간이식술을 병행하는 경향이다. 본 증례의 환자에서는 다양한 정도의 치아변색을 보였으며 색상은 노란색에서부터 짙은 초록색까지 다양했다. 법랑질의 저형성증이 모든 치아에 나타났고 구강위생이 좋지 않았으며 다발성 우식증을 가지고 있었다. Biliary atresia is defined as a complete obstruction of bile flow owing to destruction or absence of all or part of the extrahepatic bile ducts. This disease is occurring in approximately 1:10,000 live births and moderate pre-dominance of female is noted. The etiology of biliary atresia remained unsolved. The signs and symptoms are hyperbilirubinemia, jaundice, clay-colored stools, steatorrhea, dark yellow urine and hepatomegaly. Currently biliary atresia is best managed by hepatic portoenterostomy with or without liver transplantation. Biliary atresia patients with these cases showed staining of the teeth. The stains ranged in color from yellowish-brown to deep green. Enamel hypoplasia was all erupted teeth present. Patients had poor oral hygiene and rampant caries.

농산물중의 잔류농약 실태에 관한 연구(Ⅰ) : 고체상 추출에 의한 농산물중 잔류농약 분석에 관한 연구 Analysis of Residual Pesticides in Agricultural Products by Solid-Phase Extraction

허옥순,이재관,이정성,서정혁,주인선,허수정,김세은,김정근,신영희,유미자,김지연,심규창,김성환 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

신속 · 정밀하고 효율적인 짠류농약 검출을 위하여 극싱칼럼 (Extrelut-3')을 사옹한 SPE법을 시도하였다. 시료는 채소류체 사용하는 농약중 잔류성이 있고 식품공전상 시험 전처리가 각각 다른 Chlorothalonil등 6종의 농약을 선정하고 그들 표준액에 대한 PPIR수준의 농도로추출용라의 종류 및 용매량 등의 추출조건을 달리하여 회수율 등을 측정한 결과 최적의 분석조건을 얻었다. 1) 추출 용매량은 증가할수록 추출 수율이 높았고,용매 종류에 대한 추출수율은 각 농약별로 다소 차이는 있었으나 극성 정도가 비교적 높은 Ethyl acetate애서 평균 추출수율이 가장 높았다 2) 최대 평균회수율은 Ethyl acetate 60mL를 사웅할 경우로서 93.6%였다. 3)표준액을 시료에 첨가한 시험에서도 역시 Ethyl ace늘to가 푼출수율이 가장 높았으며 불순물 분리제거 효과도 LLE법 보다 월등히 우수하였다. 4) 각 농약별 검출한계는 치소 0.001ppin으로 농산물 중 미량 잔류하는 농약을 검출할 수 있을 것으로 사료된파. 그러므로 SPE법은 LLE법보다 경제적인 방법으로 판명되었으며, 앞으로 농산물 중 잔류농약 분석에서 LEE법을 대치할 쑤 있을 것으로 기대된다. Solid-phase extraction by polar column(Extrelut-3') was attempted to develope the fast and efficient method of detecting pesticide of farm product.5·ix kinds of pesticide used in farming fieldfrequently and different in pretreatment, were chosen from Korea Food Cord. Optfmal analysis condi-tions were determiBed from t31e recovery rate of standard pesticides according to extraction solvent andextraction volurae. Extraction yietd was increased as solvent polarity and extraction volume. Maximumrecovery rate was acquired at 93.6% when 60mL of ethyl acetate was used as eluent. Tllis method wasmore effective than liquid-liquid partition extract·ion method to eliminated the impurity and had 0.001ppm of detection limit. Therefore. solid-phase eEl=raction was expected to be economical substitute fortraditional liquid-liquid extT·action method.

Perceived Stress and Frailty in Older Adults

Se Hui Lee,신진영,Sarang Um,Hye Ri Shin,Young Sun Kim,최재경 대한노인병학회 2023 Annals of geriatric medicine and research Vol.27 No.4

Background: Individuals with frailty are susceptible to adverse events. Although a psychological correlation with frailty has been observed, few studies have investigated the link between stress and frailty. This study examined the association between perceived stress and frailty in older adults. Methods: This cross-sectional observational study included participants recruited between September 2021 and January 2022. The Korean version of the Perceived Stress Scale-10 was used to measure stress levels, while the frailty status was assessed using the Korean Frailty Index. Loneliness, depression, and satisfaction were measured using the UCLA Loneliness Scale, Centre for Epidemiological Studies Depression Scale, and Satisfaction with Life Scale, respectively. We used multinomial logistic regression to compare the variables between frail and robust participants. Results: Among 862 study participants (mean age, 73.62 years; 65.5% women), the mean PSS-10 score was 15.26, 10.8% were frail, 22.4% were pre-frail, and 66.8% were robust. Perceived stress was significantly associated with pre-frailty (crude odds ratio [OR]=1.147; 95% confidence interval [CI], 1.093–1.204) and frailty (crude OR=1.417; 95% CI, 1.322–1.520). After adjusting for sociodemographic factors, we examined the associations between perceived stress and prefrailty (adjusted OR=1.140; 95% CI, 1.084–1.199) and frailty (adjusted OR=1.409; 95% CI, 1.308–1.518). After adjusting for all variables, including loneliness, depression, and satisfaction, perceived stress was significantly associated with frailty (adjusted OR=1.172; 95% CI, 1.071–1.283), however, insufficient statistical evidence was observed for pre-frailty (adjusted OR=1.022; 95% CI, 0.961–1.086). Conclusion: Higher levels of perceived stress were associated with frailty in older adults. Stress management efforts may help improve frailty in this population.

Citrus unshiu Peel water extract suppresses both acute gastritis and ulcerative colitis via the regulation of PI3K/Akt signaling pathway

( Se Hui Lee ) 대구한의대학교 제한동의학술원 2021 제한동의학술원논문집 Vol.19 No.1

Gastrointestinal (GI) diseases are caused by drugs, inflammation, and external stimuli, starting with digestive and absorption dysfunction of the digestive system, including both stomach and intestines. Other factors include alcohol, acid reflux, smoking, stress, and eating habits. GI diseases, including gastritis and colitis, start with mild symptoms and cause intractable or severe symptoms. Since the treatment of these gastrointestinal diseases requires a long period of time, side effects due to long-term use of the therapeutic agent are a problem. Recently, as an alternative to minimize these side effects, the focus is on the development of natural therapeutics. Therefore, in this study, we tried to verify the effect of Citrus unshiu Peel water extract (CP) on acute gastritis (AG) and ulcerative colitis (UC) mice. First, in order to investigate the antioxidant capacity of CP, total polyphenol and total flavonoid contents and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2’-azinobis-3-ethyl-enzothiazoline-6-sulfonic acid (ABTS) radical scavenging activity were analyzed. After confirming the antioxidant activity of CP, animal experiments were conducted. The effect of CP on gastric mucosal damage was analyzed in the AG model, and the effect of CP on changes in body weight and colon length was analyzed in the UC model. In addition, the levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) and myeloperoxidase (MPO), an inflammatory biomarker, were confirmed using the serum. Reactive oxygen species (ROS) and malondialdehyde (MDA), which are oxidative stress index analysis, were identified in serum and tissue, respectively. As a result, the CP administration reduced the levels of GOT, GPT, MPO, and ROS in the serum and MDA in tissue. Furthermore, CP treatment reduced NADPH oxidase proteins that produce ROS. In addition, the expression of MAPK induced by c-Fos and c-Jun transcription factors and the expression of inflammatory mediators and pro-inflammatory cytokines induced by NF-κBp65 activation was significantly reduced through inactivation of the PI3K/Akt signaling pathway. Besides, CP administration inhibited the apoptotic proteins Bax and caspase-3 in DSS-induced ulcerative colitis mice, while increasing the expression of the anti-apoptotic proteins Bcl-2 and survivin. Taken together, these results suggest that CP administration can alleviate acute gastritis and ulcerative colitis by regulating the expression of inflammatory proteins through the inactivation of the PI3K/Akt signaling pathway.

Causes and classification of male infertility in Korea

Lee, Hui Dai,Lee, Hyo Serk,Park, Se Hwan,Jo, Dae Gi,Choe, Jin Ho,Lee, Joong Shik,Seo, Ju Tae The Korean Society for Reproductive Medicine 2012 Clinical and Experimental Reproductive Medicine Vol.39 No.4

Objective: The aim of this study is to investigate the various causes of male infertility using multiple approaches. Methods: Nine-hundred-twenty infertile male patients were analyzed at their first visit with one physician between January 1 and December 31, 2009. All patients were subjected to physical examination and semen analysis and azoospermic patients underwent hormonal testing, chromosomal tests, and testicular biopsy. Semen analysis was based on the definition of the World Health Organization. Results: Among the 920 patients, 555 patients (60.3%) had semen results within the normal range, 269 patients (29.2%) within the abnormal range, and 96 (10.5%) were diagnosed with azoospermia. Varicoceles were diagnosed in 84 of the 555 normal-range patients (15.1%) and in 113 of the 269 abnormal-range patients (42.0%). Of the 96 patients with azoospermia, 24 patients (25%) were diagnosed with obstructive azoospermia, 68 patients (71%) with non-obstructive azoospermia, and 4 patients (4%) with retrograde ejaculation. Conclusion: Various causes of male infertility have been reported and diverse treatment methods can be adopted for each cause. In this regard, research must be conducted on a larger number of patients to accurately assess the various causes of infertility in Korean patients and to investigate various infertility treatment methods.

Diversity and community analysis of fermenting bacteria isolated from eight major Korean fermented foods using arbitrary-primed PCR and 16S rRNA gene sequencing

Lee, Se-Hui,Ahn, Min-Ju,Hong, Ji-Sang,Lee, Ju-Hoon The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.3

Korean fermented foods are known to be beneficial for human health. Bacterial community studies have been conducted to figure out what roles the bacteria used to ferment these foods play in food fermentation. The metagenomic approach identifies both culturable and unculturable bacterial compositions, but this technique is limited in its ability to accurately determine the bacterial species from short 16S rRNA PCR products. In this study, we revisited the culture-dependent method using a relatively large number of bacterial isolates in an attempt to overcome the problem of bacterial identification, accepting that the unculturable bacterial population in each fermented food would be undetectable. Eight Korean fermented foods including kimchi, jeotgal, and meju were collected, and 1589 fermenting bacterial strains were randomly isolated. Bacteria were grouped by banding pattern using arbitrary-primed (AP) PCR prior to bacterial identification and sorted into 219 groups; 351 strains were not grouped because there was no identical AP-PCR band pattern. 16S rRNA sequence analysis identified the bacterial compositions of the fermented foods. As dominant genera, Lactobacillus and Leuconostoc strains were detected in four kimchi samples, Staphylococcus in three jeotgal samples, and Enterococcus and Bacillus in the meju sample. Interestingly, S. Equorum was most dominant in saeu-jeotgal, indicating that it is halophilic and may enhance the fermentation flavor. Further comparative analysis of this study with previous metagenomic results revealed that bacterial communities in fermented foods are highly similar at the genus level but often differ at the species level. This bacterial community study is useful for understanding the roles and functional properties of fermenting bacteria in the fermentation process of Korean fermented foods.

Screening for anti-inflammation and anti-oxidant effects of mongolian plant extracts

Se Hui Lee,Mi-Rae Shin,Seong-Soo Roh 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The purpose of this study is to find anti-inflammatory candidates by screening sample of 40 Mongolian medicinal crops. All the samples were tested the anti-inflammatory activity, such as production of nitric oxide (NO) in lipopolysaccharide (LPS)-induced Raw264.7 macrophages. In addition, the anti-oxidant activity of all the samples were analyzed by DPPH and ABTS-free radical scavenging assays (inhibition ratio at 50 μg/ml concentration), total polyphenol, and total flavonoid contents. Of the 40 samples, the inhibition of NO production showed excellent efficacy in the order of Paeonia anomala L, Crataegus sanguinea Pall, Betulla phlatyphilla, Parnassia palustris L, and Veronica incana. However, when considering the NO inhibition rate and anti-oxidant effects totally, the top three species were Artemisia frigida Willd, Cotoneaster Мongolicus (Rosaceae), and Pinus sylvestris (Pinaceae). Especially, 3 samples were significantly suppressed NO production in a concentration-dependent manner in Raw 264.7 cells. Therefore, 3 samples were showed outstanding effects in the analysis of anti-oxidant activity, such as DPPH and ABTS free radical assays, total polyphenol, and total flavonoid contents. Taken together, these results suggest that Artemisia frigida Willd, Cotoneaster Мongolicus (Rosaceae), and Pinus sylvestris (Pinaceae) could alleviated the inflammatory response through NO suppression and anti-oxidant activity, and may be used for the prevention and treatment of inflammatory diseases including osteoarthritis. But, further research should be accomplished the efficacy and the underlying mechanism in vivo experiment to use safely.

Physicochemical and Sensory Properties of Baikseolgi Substituted with Strawberry Powders

( Se Ri Lee ),( Ji Eun Jeon ),( Hui Jeong Kim ),( Jun Ho Lee ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2008 한국식품저장유통학회 학술대회 Vol.2008 No.-

The benzodiazepine anesthetic midazolam prevents hyperglycemia‐induced microvascular leakage in the retinas of diabetic mice

Lee, Yeon-Ju,Kim, Minsoo,Lee, Jee-Yeon,Jung, Se-Hui,Jeon, Hye-Yoon,Lee, Seung Ah,Kang, Seongsik,Han, Eun-Taek,Park, Won Sun,Hong, Seok-Ho,Kim, Young-Myeong,Ha, Kwon-Soo Federation of American Society for Experimental Bi 2018 The FASEB Journal Vol.32 No.11

Rosa hybrida extract suppresses vascular smooth muscle cell responses by the targeting of signaling pathways, cell cycle regulation and matrix metalloproteinase-9 expression

LEE, SE-JUNG,WON, SE YEON,PARK, SUNG LYEA,SONG, JUN-HUI,NOH, DAE-HWA,KIM, HONG-MAN,YIN, CHANG SHIK,KIM, WUN-JAE,MOON, SUNG-KWON UNKNOWN 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.4

<P>The pharmacological effects of Rosa hybrida are well known in the cosmetics industry. However, the role of Rosa hybrida in cardiovascular biology had not previously been investigated, to the best of our knowledge. The aim of the present study was to elucidate the effect of water extract of Rosa hybrida (WERH) on platelet-derived growth factor (PDGF)-stimulated vascular smooth muscle cells (VSMCs). VSMC proliferation, which was stimulated by PDGF, was inhibited in a non-toxic manner by WERH treatment, which also diminished the phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and AKT. Treatment with WERH also induced G1-phase cell cycle arrest, which was due to the decreased expression of cyclins and cyclin-dependent kinases (CDKs), and induced p21WAF1 expression in PDGF-stimulated VSMCs. Moreover, WERH treatment suppressed the migration and invasion of VSMCs stimulated with PDGF. Treatment with WERH abolished the expression of matrix metalloproteinase-9 (MMP-9) and decreased the binding activity of nuclear factor-kappa B (NF-kappa B), activator protein-1 (AP-1), and specificity protein 1 (Sp1) motifs in PDGF-stimulated VSMCs. WERH treatment inhibited the proliferation of PDGF-stimulated VSMCs through p21WAF1-mediated G1-phase cell cycle arrest, by decreasing the kinase activity of cyclin/CDK complexes. Furthermore, WERH suppressed the PDGF-induced phosphorylation of ERK1/2 and AKT in VSMCs. Finally, treatment with WERH impeded the migration and invasion of VSMCs stimulated by PDGF by downregulating MMP-9 expression and a reduction in NF-kappa B, AP-1 and Sp1 activity. These results provide new insights into the effects of WERH on PDGF-stimulated VSMCs, and we suggest that WERH has the potential to act as a novel agent for the prevention and/or treatment of vascular diseases.</P>