Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters

Lee, Eun-Sol,Lee, Dong-Sung,Pandeya, Prakash Raj,Kim, Youn-Chul,Kang, Dae-Gil,Lee, Ho-Sub,Oh, Byung-Chul,Lee, Dae Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

Sodium butyrate (SB) has various metabolic actions. However, its effect on dipeptidyl peptidase 4 (DPP-4) needs to be studied further. We aimed to evaluate the metabolic actions of SB, considering its physiologically relevant concentration. We evaluated the effect of SB on regulation of DPP-4 and its other metabolic actions, both in vitro (HepG2 cells and mouse mesangial cells) and in vivo (high fat diet [HFD]-induced obese mice). Ten-week HFD-induced obese C57BL/6J mice were subjected to SB treatment by adding SB to HFD which was maintained for an additional 16 weeks. In HepG2 cells, SB suppressed DPP-4 activity and expression at sub-molar concentrations, whereas it increased DPP-4 activity at a concentration of $1,000{\mu}M$. In HFD-induced obese mice, SB decreased blood glucose, serum levels of insulin and $IL-1{\beta}$, and DPP-4 activity, and suppressed the increase in body weight. On the contrary, various tissues including liver, kidney, and peripheral blood cells showed variable responses of DPP-4 to SB. Especially in the kidney, although DPP-4 activity was decreased by SB in HFD-induced obese mice, it caused an increase in mRNA expression of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. The pro-inflammatory actions of SB in the kidney of HFD-induced obese mice were recapitulated by cultured mesangial cell experiments, in which SB stimulated the secretion of several cytokines from cells. Our results showed that SB has differential actions according to its treatment dose and the type of cells and tissues. Thus, further studies are required to evaluate its therapeutic relevance in metabolic diseases including diabetes and obesity.

체육수업의 재미요인이 학생들의 심리적 유인성향에 미치는 영향

이종철(Lee Jong-Chul),조홍식(Cho Hong-Sik),박윤혁(Park Yoon-Hyuk),이상섭(Lee Sang-Sub) 한국체육과학회 2006 한국체육과학회지 Vol.15 No.3

The purpose of this study was to explore ways to at identifying what factors made students felt enjoyment during physical education class and how much those factors influence on psychological of inducement inclination. To this end, Cluster Random Sampling was used to set up the population with boys and girls middle school students in Kyunggi-do and Inchon-si area. The survey tool was questionnaire and Collected data hereof were analyzed by one way ANOVA analysis and Multiple Regression Analysis with SPSS WIN Ver 11.5. The aforementioned method and procedure led this research to the conclusion as follows. Firstly, out of sub-factors of physical education class enyoyment by middle schoolers' individual characteristics(gender, result), boy students showed more than girl students, in which there were statistically significant difference. Meanwhile, statistically significant difference was not found in free time, explanation and modeling. Secondly, out of sub-factors of psychological of inducement inclination by middle schoolers' individual characteristics(gender, result), boy students' response was higher in senses of confidence, enthusiasm, accomplishment and indulgence than girl students' in which all considers appeared. Thirdly, the effect enjoymant factors of physical education class had statistically significant on sub-factors of psychological of inducement inclination senses of confidence, enthusiasm, accomplishment and indulgence, free time that is a sub-factor of enjoymant which did not had statistically significant influenced on sense of accomplishment.

체육수업에 대한 교과 흥미도가 학생들의 학업열의에 미치는 영향

이상섭(Lee, Sang-Sub),조홍식(Cho, Hong-Sik) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.1

The purpose of this study was to investigate the effects of subject interest in physical education classes on academic engagement among students. For that purpose, the investigator found the population in middle and high school students in Gyeonggi Province and sampled 420 students through convenience sampling. Collected data were put to multiple regression analysis with the PASW Version 18 .0 statistical program. The findings were as follows: First, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(efficacy). Second, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(commitment) . Third, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (vitality). Finally, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (absorption)

체육수업에 대한 교과 흥미도가 학생들의 기본적 심리욕구에 미치는 영향

이종철(Lee, Jong-Chul),이상섭(Lee, Sang-Sub) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.6

The purpose of this study is to identify effects of subject interest in physical education on basic psychological desires of students. For doing this, the authors established the students in middle and high schools in Gyeonggi Province as the population, selecting 340 students by using convenience sampling. The collected data was conducted of multiple regression analysis by using the PASW Ver 18.0 statistic program, and the results are as follows. First, the subject interest in physical education had statistically significant effects on the basic psychological desires of the students(competence), but among the sub-factors, teacher preference factors did not have significant effects on the basic psychological desires(competence). Second, all the sub-factors of the subject interest in physical education(teacher preference, subject content, values & efforts, and subject competence) had statistically significant effects on the basic psychological desires of the students(relation). Third, the subject interest in physical education had statistically significant effects on the basic psychological desires of the students(autonomy), but among the sub-factors, teacher preference factors did not have significant effects on the basic psychological desires(autonomy).

철도시스템분야 실무적용을 위한 RAM Guidance 개발에 관한 연구

이중섭(Joong Sub31 Lee),백영구(Young Goo Baek),이진(Jin Lee),한미정(Mi Jung Han),김성환(Sung Hwan Kim) 한국철도학회 2015 한국철도학회 학술발표대회논문집 Vol.2015 No.10

철도시스템 기술 고도화 및 복잡성의 증대와 더불어 발주기관으로부터 시스템의 성능보증과 안전성 확보가 요구되고 있는 실정이다. 또한 국내 철도안전법 강화로 인하여 시스템 보증활동의 중요성이 증가되고 있다. 본 논문에서는 국제철도관련 규격을 바탕으로 철도시스템의 RAM 업무에 대한 절차를 정의하고, 해당 철도 프로젝트에서 RAM 업무를 원활히 수행할 수 있도록 관련된 국제 규격 및 유사시스템 적용 사례에 기반하여 철도시스템 개발 특성을 고려한 RAM 업무 수행 지침(Guidance)을 개발하였다. Performance and safety assurances for the railway system are required by the client because of the high degree of technology and increased complexity. In addition, as a result of reinforced domestic railway safety laws, the importance of system assurance activity has been growing steadily. This paper defines the task of the RAM process based on international railway industry standards for RAMS engineers to work efficiently and develops the RAM guidance considering the railway system characteristics based on domestic standards and application cases of railway systems.

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

Anti-Cancer Effect of Ginsenoside F₂ against Glioblastoma Multiforme in Xenograft Model in SD Rats

Shin, Ji-Yon,Lee, Jung-Min,Shin, Heon-Sub,Park, Sang-Yong,Yang, Jung-Eun,KimCho, So-Mi,Yi, Tae-Hoo The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.1

The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside $F_2$ (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with $IC_{50}$ of 50 ${\mu}g/mL$ through apoptosis, confirmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 mg/kg weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer.

하천 복원을 위한 과거 및 현재 자료 기반의 하천지형학적 특성 분석: 미호천과 내성천을 중심으로

이찬주,김지성,김규호,신형섭,Lee, Chan Joo,Kim, Ji Sung,Kim, Kyu Ho,Shin, Hyoung Sub 한국수자원학회 2015 한국수자원학회논문집 Vol.48 No.3

하천 복원을 위한 기초 작업으로서 자연 상태에 가까운 하천의 지형학적 특성을 파악하기 위해 과거 및 현재 자료를 이용하여 하천지형학적 분석을 실시하였다. 대상 하천은 미호천과 내성천이다. 분석에 포함된 지형학적 변수는 하곡변수인 곡저폭, 하도 형상변수인 사행도, 강턱하폭, 하도경사, 구성재료 변수인 하상재료, 지배 변수인 강턱유량, 비유수력이며, 지배적인 프로세스가 함께 고려되었다. 분석 결과 두 하천은 비슷한 유역면적을 갖는 모래하천이지만, 곡저폭, 하도 형상변수, 종단적 위치에 따른 지배적인 프로세스 등에서 차이가 있는 것으로 나타났다. 또한 지형학적 변수들 사이의 상호 관계를 분석하였다. 그 결과 강턱하폭은 강턱유량에 비례하여 증가하였고, 그 경향성은 기존의 연구 결과와 대체로 일치하고 있다. 강턱유량과 하도경사를 분석한 결과 미호천은 사행하천과 망상하천의 특성을 나타내고 있는 반면, 내성천은 대부분의 구간에서 망상하천에 해당하는 것으로 나타났다. 하도경사와 하폭수심비 관계를 이용하여 지배적인 프로세스를 분석한 결과 미호천은 사구-연흔의 특성을 나타내고 있으나 내성천은 하류에서는 망상하천 특성이 지배적인 반면, 상류에서는 여울-소 내지는 평하상 특성이 지배적인 것으로 나타났다. 과거 자료를 이용한 분석 결과는 정비된 하천의 자료를 이용한 분석 결과에 비해 합리적인 것으로 나타났다. As a basic work for river restoration, analysis on fluvial geomorphological characteristics is made using past and present data to understand close-to-nature geomorphic status. The Miho and the Naesung Rivers are targets of this study. Fluvial geomorphic variables including valley-floor width, sinuosity, bankfull width, channel gradient, bed material size, bankfull discharge and unit stream power are evaluated with dominant processes. Though common sand-bed rivers with similar catchment area, the Miho and the Naesung Rivers are different in terms of valley-floor width, channel shape variables and dominant processes related with longitudinal location. In addition, analyses on interrelationship among the geomorphological variables are carried. Bankfull width is shown to be proportional to bankfull discharge, as is in a rough agreement with the previous studies. Relationship of bankfull discharge and channel gradient shows meandering and braiding are prevalent in the Miho River, whereas the most of the sub-reaches of the Naesung River fall to braiding. Relationship of channel gradient with width-depth ratio indicates dune-ripple processes are dominant in the Miho River, while the Naesung River shows longitudinal diversity from braiding in the downstream sub-reaches to riffle-pool and plane-bed along the upper ones. Analyses based on the past data on a river in a close-to-nature status are thought to be rather reasonable in comparison with those on the same river in a engineered condition.

Haplogroup Classification of Korean Cattle Breeds Based on Sequence Variations of mtDNA Control Region

Kim, Jae-Hwan,Lee, Seong-Su,Kim, Seung Chang,Choi, Seong-Bok,Kim, Su-Hyun,Lee, Chang Woo,Jung, Kyoung-Sub,Kim, Eun Sung,Choi, Young-Sun,Kim, Sung-Bok,Kim, Woo Hyun,Cho, Chang-Yeon Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Many studies have reported the frequency and distribution of haplogroups among various cattle breeds for verification of their origins and genetic diversity. In this study, 318 complete sequences of the mtDNA control region from four Korean cattle breeds were used for haplogroup classification. 71 polymorphic sites and 66 haplotypes were found in these sequences. Consistent with the genetic patterns in previous reports, four haplogroups (T1, T2, T3, and T4) were identified in Korean cattle breeds. In addition, T1a, T3a, and T3b sub-haplogroups were classified. In the phylogenetic tree, each haplogroup formed an independent cluster. The frequencies of T3, T4, T1 (containing T1a), and T2 were 66%, 16%, 10%, and 8%, respectively. Especially, the T1 haplogroup contained only one haplotype and a sample. All four haplogroups were found in Chikso, Jeju black and Hanwoo. However, only the T3 and T4 haplogroups appeared in Heugu, and most Chikso populations showed a partial of four haplogroups. These results will be useful for stable conservation and efficient management of Korean cattle breeds.

Guided formation of sub-5 nm interstitial gap between plasmonic nanodisks

Jin Gyeong Son,Sang Woo Han,Jung-Sub Wi,Tae Geol Lee 한국진공학회 2015 한국진공학회 학술발표회초록집 Vol.2015 No.2