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Lactobacillus casei AHU 1055 및 Lactobacillus acidophilus JAM 1043의 凍結乾燥와 生存率에 關한 硏究
金昌漢,金明浩,朴芝賢,李明燮,朴相瑨 건국대학교 동물자원연구센터 1992 動物資源硏究誌 Vol.17 No.-
In order to find the most suitable conditions of freeze-drying for the long term preservation of lacto-bacillus casei AHU 1055 and Lactobacillus acidophilus JAM 1043, the growth phase, the cryoprotection medium and inoculum size have been studied. The results obtained in this study are summerized as follows : 1. L. casei AHU 1055 and L.acidophilus JAM 1043 reached to stationary phase after incubating at 37˚C for 16hrs and 12hrs in MRS broth, respectively. 2. The highest viability of each strains was obtained in 11% skim-milk for L. casei AHU 1055 and 9% skim-milk for L. acidophilus JAM 1043 as basic cryoprotection medium. 3. The complex medium contained 11% skim milk powder, 2.5% arginine and 3% glucose was the best suspending midium for freeze-drying of L. casei AHU 1055, and the medium contained 9% skim milk powder, 7% glycerol, 9% lactose, 0.5% arginine was the best suspending medium for freeze-drying of L. acidophilus JAM 1043. 4. The strong positive correlation was found between inoculum size and the survival rate in both strains. 5. When the tested strains were cultured and freeze-dried under the best optimal conditions, the survival rates of freeze- dried L. casei AHU 1055 and L. acidophilus JAM 1043 were 85.8% and 84.0%, respectively.
이정규,전지현,김민정,이규재,김한겸,조현득,채양석 大韓法醫學會 1999 대한법의학회지 Vol.23 No.2
Although it is known that hypothyroidism can cause cardiac dysfunction, it is very hard to find a case report on sudden death due to hypothyroidism. There are only two reported cases on mediline; one is about a 15-year-old girl who died suddenly of Hashimoto's thyroiditis and the other is about a 31-year-old man who also died suddenly of hypothyroidism associated with chronic lymphocytic thyroiditis. Recently we found a young woman who unexpectedly died while she was sleeping. She was diagnosed as hypothyroidism when she went to hospital about a month before she died. At autopsy there were roughly four abnormal findings; Hashimoto's thyroiditis, mild chronic active hepatitis, diverticulosis and accessory spleen. Trace of chlorpheniramine was detected in gastric remains and blood alcohol level was 0.37 percent. To our knowledge, this is the first case reporting sudden and unexpected natural death associated with hypothyroidism in Korea.
Kim, Ju Yeon,Cho, Jeong Hyun,Kim, Eun Mi,Shin, Hyun-Jin,Hwang, Sang-Gu,Song, Jie-Young,Um, Hong-Duck,Park, Jong Kuk Elsevier 2019 BIOMEDICINE AND PHARMACOTHERAPY Vol.113 No.-
<P><B>Abstract</B></P> <P><B>Aims</B></P> <P>In this study, we examined whether β-apopicropodophyllin (APP) could act as a radiosensitizer in non-small cell lung cancer (NSCLC) cells.</P> <P><B>Main methods</B></P> <P>The <I>in vitro</I> radiosensitizing activity of APP was demonstrated with clonogenic assay, immunoblotting, Annexin V-Propidium iodide (PI) assay, BrdU incorporation, detection of mitochondrial ROS/intracellular of H<SUB>2</SUB>O<SUB>2</SUB>, mitochondrial membrane potential detection, and performing of isolation of mitochondrial and cytosolic fractions. The <I>in vivo</I> radiosensitizing activity of APP was determined in xenografted mice with co-treatment of APP and IR based on measurement of tumor volumes and apoptotic cell death.</P> <P><B>Key findings</B></P> <P>The results of a clonogenic assay indicated that a combination of APP and γ-ionizing radiation (IR) inhibits cell growth and increases cell death in NSCLC cells. Several signal transduction pathways were examined for their potential involvement in the apparent radiosensitization effect of APP, as assessed by immunoblotting analyses and mitochondrial potential determination <I>in vitro</I>. Treatment of NCI-H460 cells with 15 nM APP and NCI-H1299 cells with 10 nM APP yielded dose-enhancement ratios of 1.44 and 1.24, respectively. Enhanced ER stress, disrupted mitochondrial membrane potential, and increased reactive oxygen species (ROS) were observed in cells co-treated with APP and IR, and this was followed by the cytosolic release of cytochrome <I>c</I> and consequent activation of caspase-3 and -9. Notably, inhibition of JNK, which prevents caspase activation, blocked the APP/IR-induced activations of ER stress and apoptotic cell death. In NCI-H460 or NCI-H1299 cell-xenografted mice, APP/IR treatment delayed the time it took tumors to reach a threshold size by 22.38 and 16.83 days, respectively, compared with controls, to yield enhancement factors of 1.53 and 1.38, respectively.</P> <P><B>Significance</B></P> <P>APP has a radiosensitizing function derived from its ability to induce apoptotic cell death <I>via</I> activation of ER stress, disruption of mitochondrial membrane potential, and induction of the caspase pathway.</P> <P><B>Highlights</B></P> <P> <UL> <LI> β-Apopicropodophyllin is a candidate drug for treatment of non-small cell lung cancer. </LI> <LI> Novel function of β-Apopicropodophyllin as a radiosensitizer was confirmed <I>in vitro</I> and <I>in vivo.</I> </LI> <LI> Radiosensiting effect of β-Apopicropodophyllin is exerted by ER stress-mediated apoptosis <I>via</I> JNK activation. </LI> </UL> </P>
Strategy for Curative Endoscopic Resection of Undifferentiated-Type Early Gastric Cancer
Jie-Hyun Kim 대한소화기내시경학회 2019 Clinical Endoscopy Vol.52 No.1
Endoscopic resection (ER) of undifferentiated-type early gastric cancer (UD-EGC) has a lower curative resection (CR) rate thandifferentiated-type EGC. However, if UD-EGC is curatively resected using ER, long-term outcomes can be favorable. Thus, the strategyfor CR by ER is important in UD-EGC. To achieve CR in UD-EGC, biological behaviors including tumor growth patterns must beconsidered. This review aims to describe what is important for curative ER of UD-EGC.
Kim, Sun Jie,Kim, Wook Hyun,Lee, Chang-Hyun,Kim, Hwa-Min,Kang, Cheon Su,Han, Yoon Soo Informa UK (TaylorFrancis) 2017 MOLECULAR CRYSTALS AND LIQUID CRYSTALS - Vol.651 No.1
<P>Effects of sodium ethanesulfonate (SES) adsorbed on photoelectrode surface on the performance of dye-sensitized solar cells (DSSCs) was investigated. SES-modified photoelectrode was prepared by soaking the TiO2 film to an aqueous SES solution for 20 min. The DSSC with SES-modified TiO2 layer showed an increase in open circuit voltage (V-oc), short circuit current (J(sc)) and fill factor (FF), resulting in a power conversion efficiency of 11.20%, compared to that of reference device with bare TiO2 (10.21%). Electrochemical impedance spectroscopy and open circuit voltage decay measurements revealed that the SES modification of TiO2 surface led to a longer electron lifetime by the suppression of the interfacial charge recombination between photo-injected electrons and I-3(-) ions, resulting in an increase in J(sc) and V-oc, compared with those of a reference device without surface modification.</P>