Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps Sinensis in the Bronchoalveolar Lavage Fluids Cells

Kuo, Yuh-Chi,Wu, Chia-Lin,Tasi, Wei-Jern,Wang, Jir-Yenn,Chang, Shi-Chung,Lin, Ching-Yuang,Shiao, Ming-Shi 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang₃,Ching-Yung Lin⁴,and Ming-Shi Shiao⁴¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan Yuh-Chi Kuo¹,Chia-Lin Wn¹,Wei-jern Tasi¹,Jir-Yenn Wang²,Shi-Chung Chang²,Ching-Yung Lin³,and Ming-Shi Shiao⁴.¹National Research Institute of Chinese Medicine, ²Department of Biology,Fu Jen University, ³Department of Chest, ⁴Department of Pediatrics, □De[art,amt of Medical Research and Education. Taipei Veterans General Hospital, Taipei, Taiwan. Blocking of Cell Proliferation, Cytokines Production and Genes Expression Following Administration of Cordyceps sinensis in the Bronchoalveolar Lavage Fluids Cells. Proceedings of international Symposium on East-West Medicine, Seoul. 44-64, 1999.-Cordyceps sinensis is a major parasitic fungus on larva of Lepidoptera. It is one of the well know fungi used in traditional Chinese medicine for treatment asthma, lung and kideney diseases. Many fungi belonging to the genus Cordyceps have been demonstrated to produce natural products with various biological activities. Reported biological activities of genus cordyceps include:(a)inhibition of DNA and RNA synthesis;(b)enhancement of cell differentiation;(c)restruction of cytoskeleton;(d)inhibition of protein kinase activity;(e)antitumor activity on bladder, colon, lung carcinoma as well as fibroblastoma;(f)inhibition of the infection and revers transciptase activity of human immunodeficiency virus type 1; and(g) inhibition of methylation of nucleic acid. Thus, it is suggested that the extracts of Cordyceps sp. Contain antitumor, antiviral, and many other biological activities. In our previous studies, we proved that C. sinensis contains the antitumor and immunomodulatory agents. In the present studies, effects of C. sinensis on bronchoalveolar lavage fluids(BAL)cells were demonstrated. The crude methanolic extracts of C. sinensis were fractionated into 15 fractions by silica gel column chromatography. Effects of various fraction on BAL cells proliferation and lipopolysaccharide(LPS) stimulated interleukin-1β(IL-8),platelet activating factor(PAF), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α),and interleukin-8(IL-8) production on BAL cells were determined. The result indicated that the CS-19-22 fraction suppressed BAL cells proliferation activated by LPS. The median inhibitory concentration(IC50) was 6㎍/ml. The CS-19-22 fraction also decreased IL-1β,PAF,IL-6, TNF-αand IL-8 production.The results of revers transcription-polymerase chain reaction(RT-PCR) demonstrated that the CS-19-22 fraction did not affect IL-1β,PAF,IL-6, TNF-α,and IL-8 mRNAs expression in BAL cells activated by LPS. By contrast, it inhibited the IL-10 mRNA expression but enhanced IFN-γand IL-12 mRNAs expression in activated BAL cells. Moreover, the CS-19-22 fraction blocked PAF-induced platelet aggregation. It is unlikely that cytotoxicity was involved. Because no cell deths were observable. We hypothesize that inhibitory mechanisms of CS-19-22 on BAL cells proliferation may be related to the impairments of gene expression and production of cytokines in BAL cells. The explanation of C. sinensis antiasthma function may involved the following(1)C.sinenesis contained immunomodulatory agents that stimulated IFN-γand IL-12 expression in TH1cells.(2)Enhanced secretion of IFN-γand IL-12 will inhibit the Tγcells immune responses Ex. Decreasing of IL-6 and IL-10 production.(3) The impairments of cytokines production in TH2 cells suppressed th BAL cells proliferation then more cytokines production Ex.IL-1β,PAF,IL-6,TNF-αand IL-8 in activated BAL cells were decreased. (4)The suppression of TH2cells immune responses will inhibit the growth and differentiation of B cells then IgE production will be decreased. (5)Reduced production of IgE will decreased asthma attack occurring in individuals. However, the results of the present study suggested that C. sinensis may also have acted to treat asthma in part by inhibiting BAL cells proliferation and cytokine gene expression and production. Future, experiments with treatment of animals with asthma with C. sinensis will be necessary to defined whether C. sinensis can reduce asthma injury. Plans are underway for the isolation of pure principal growth inhibitors and their mechanisms of action are subjected for further study.

Transcatheter Embolization of a Ruptured Internal Pudendal Artery Pseudoaneurysm in a Patient with Neurofibromatosis Type 1

Chang-Wei Zhang,Zhi-Gang Yang,Xiao-Dong Xie,Chao-Hua Wang,Chao You,Wei Li 대한의학회 2010 Journal of Korean medical science Vol.25 No.4

Neurofibromatosis type 1 (NF-1) is an autosomal dominant disorder. Pseudoaneurysms formation and rupture is an unusual complication of neurofibromatosis. To date, pseudoaneurysm of the internal pudendal artery associated with NF-1 has not been reported. In this article, we present a 62-yr-old man with NF-1 suffering from spontaneous hematoma of the perinea and scrotum. A digital substraction angiography disclosed a ruptured pseudoaneurysm of the right internal pudendal artery, which was successfully managed with transcatheter embolization.

A thiazoline-containing cobalt(II) complex based colorimetric fluorescent probe: “turn-on” detection of fluoride

Wang, Jing,Liu, Hai-Bo,Wang, Wei,Kim, Il,Ha, Chang-Sik Royal Society of Chemistry 2009 Dalton Transactions Vol.2009 No.47

<P>A new thiazoline-containing cobalt(II) complex displayed colorimetric and fluorescent “turn-on” selectivity and sensitivity toward fluoride anions compared to the other anions investigated through color changes from pink to blue and changes in the emission spectra at approximately 570 nm when excited at 520 nm.</P> <P>Graphic Abstract</P><P>A new thiazoline-containing cobalt complex showing selectivity to fluoride anion was investigated and a significant light pink to dark blue color change was observed upon the addition of fluoride ions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b918887h'> </P>

Mitochondria-mediated Apoptosis in Human Lung Cancer A549 Cells by 4-Methylsulfinyl-3-butenyl Isothiocyanate from Radish Seeds

Wang, Nan,Wang, Wei,Huo, Po,Liu, Cai-Qin,Jin, Jian-Chang,Shen, Lian-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

4-Methylsulfinyl-3-butenyl isothiocyanate (MTBITC) found in the radish (Raphanus sativus L.), is a wellknown anticancer agent. In this study, the mechanisms of the MTBITC induction of cell apoptosis in human A549 lung cancer cells were investigated. Our PI staining results showed that MTBITC treatment significantly increased the apoptotic sub-G1 fraction in a dose-dependent manner. The mechanism of apoptosis induced by MTBITC was investigated by testing the change of mitochondrial membrane potential (${\Delta}{\Psi}m$), the expression of mRNAs of apoptosis-related genes by RT-PCR, and the activities of caspase-3 and -9 by caspase colorimetric assay. MTBITC treatment decreased mitochondrial membrane potential by down-regulating the rate of Bcl-2/Bax and Bcl-xL/Bax, and activation of caspase-3 and -9. Therefore, mitochondrial pathway and Bcl-2 gene family could be involved in the mechanisms of A549 cell apoptosis induced by MTBITC.

Drilling Temperature and Hole Quality in Drilling of CFRP/Aluminum Stacks Using Diamond Coated Drill

Chang-Ying Wang,Yu-Han Chen,Qinglong An,Xiaojiang Cai,Wei-Wei Ming,Ming Chen 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Composite/metal stack materials, most often consisting of carbon fiber reinforced polymer (CFRP) composites and aluminum or titanium alloy (Al or Ti), have been widely used in the aviation industry. It is still a challenge to make holes on the composite/metal stack materials with high quality. This paper aims to investigate the influence of drilling parameters on the drilling of stack materials consisting of T800/X850 CFRP and 7075-T651 Al with regard to drilling force, drilling temperature, hole diameter and hole surface quality. Diamond coated drill bits with double point angles were employed in the drilling tests. The drilling temperature was measured utilizing a rotational motion temperature measuring system. It is indicated that maximum drilling temperature increases with the increase of spindle speed, while, it decreases with increasing feed rate. The thrust force and drilling temperature increase abnormally during drilling of the Al layer, which can be attributed to the evacuation difficulty of the long and flexible Al chips. It is found that sudden drops in the surface profile of CFRP are caused by the cavities owning to the resin degradation effects. The influences of different stack sequences on the drilling temperature and hole surface quality have also been discussed.

Reformability evaluation of blasting-enhanced permeability in in situ leaching mining of low-permeability sandstone-type uranium deposits

Wang Wei,Liang Xuanyu,Niu Qinghe,Wang Qizhi,Zhuo Jinyi,Su Xuebin,Zhou Genmao,Zhao Lixin,Yuan Wei,Chang Jiangfang,Zheng Yongxiang,Pan Jienan,Wang Zhenzhi,Ji Zhongmin 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.8

It is essential to evaluate the blasting-enhanced permeability (BEP) feasibility of a low-permeability sandstone-type uranium deposit. In this work, the mineral composition, reservoir physical properties and rock mechanical properties of samples from sandstone-type uranium deposits were first measured. Then, the reformability evaluation method was established by the analytic hierarchy process-entropy weight method (AHP-EWM) and the fuzzy mathematics method. Finally, evaluation results were verified by the split Hopkinson Pressure Bar (SHPB) experiment and permeability test. Results show that medium sandstone, argillaceous sandstone and siltstone exhibit excellent reformability, followed by coarse sandstone and fine sandstone, while the reformability of sandy mudstone is poor and is not able to accept BEP reservoir stimulation. The permeability improvement and the distribution of damage fractures before and after the SHPB experiment confirm the correctness of evaluation results. This research provides a reformability evaluation method for the BEP of the low-permeability sandstone-type uranium deposit, which contributes to the selection of the appropriate regional and stratigraphic horizon of the BEP and the enhanced ISL of the low-permeability sandstone-type uranium deposit.

중일 도서 영유권 분쟁 원인에 대한 중국 국내 전문가 인식조사

왕유위 ( Wei-wei Wang ),남창희 ( Chang-hee Nam ) 한일군사문화학회 2016 한일군사문화연구 Vol.21 No.-

본 논문은 조어도/센카쿠에 대한 중국 해양 영유권 분쟁원인의 실체를 파악하기 위해 문헌자료 분석과 전문가 브레인스토밍 기법을 통하여 경합모델군을 도출하고 역사적 정체성 추적을 통해 분쟁의 정체성 구조와 맥락을 파악하고자 하였다. 이어서 현지 심층 면접을 통해 경합 모델군들에 대한 설득력을 비교 평가하기 위한 전문가 인식조사를 시도하였다. 센카쿠/댜오위다오 분쟁 원인을 밝히기 위해 세 가지 모델, ``중국포위망 마찰론``, ``중국해상 권익 정체성 확대론`` 그리고 ``관심전환이론``을 현지에서 5개월간 북경, 샤면, 광주, 무한 등 주요 도시에서 중국 국내 전문가를 심층면접하여 조사하였다. 주어진 샘플 내에서 중국인 안보전문가들에게 센카쿠/댜오위다오 분쟁에 있어서 세 가지 가설이론의 설득력 정도는 해양권익 정체성 확대론이 가장 높고, 가장 낮은 것은 관심전환이론이며 중국포위망 마찰론은 그 중간에 위치하였다. 현지 인터뷰에 응한 대부분의 중국학자들은 관심전환이론에는 공감하지 않았지만 일부 학자들은 관심전환이론이 중국을 설명하는데 일부 타당한 측면이 있다고 인식하였다. 이러한 조사결과는 미국과 일본의 전문가들과의 견해와는 편차가 있을 수 있으나 이 결론은 최소한 중국 전문가들의 인식구조에 대해서는 신뢰할 만한 것으로 보인다. 또한 이를 통해 향후 중국 정부가 이 문제에 접근하는 정체성과 정책방향을 가늠하는 데 도움이 될 것이다. This paper presents an expert survey research conducted to security experts in China on maritime territorial disputes over small islands located between China, Taiwan, and Japan. Three models explaining the increasingly volatile disputes over Senkaku/Diaodao islands were derived from the literature survey and expert brainstorming process. They include China containment friction model, maritime interest identity expansion model, and domestic tension diversion model. Maritime interest identity widely shared among the Chinese was detailed in the historical review of the birth, development and current status of dispute. Japan``s Abe cabinet has taken a series of security measures including reinterpretation of Clause 9 of the Constitution, which international observers see as also having something to do with growing territorial tension with Beijing. After an extensive field work in Zhengzhou, Beijing, Xiamen, Wuhan, and Gwangzhou, the survey result revealed a distinctively dominant support among Chinese security experts to the second model, namely, China``s maritime interest identity expansion model. The first, China containment friction model, won the second large number of supporters while the last model was not welcome by the Chinese researchers. The simple statistical table does not necessarily reflect the true, international view of the issues, yet it may at least represent the way Chinese security experts perceive as the causes of conflict. The significance of this type of study lies in that it may explain and predict Chinese policy orientation with respect to the maritime territorial dispute and even future Sino-Japanese bilateral relations.

미국의 공해전투(AirSea Battle) 개념과 미일동맹

남창희 ( Chang Hee Nam ),왕유위 ( Wei Wei Wang ) 한일군사문화학회 2012 한일군사문화연구 Vol.14 No.-

A noticeably rapid missile force development of the PLA poses a new challenge to those all who benefit from open and free global commons (SLOC, air, and space) in the Pacific Ocean. Washington warily estimates that the PLA`s capabilities going beyond the Cross-Strait contingency aims at constraining America`s power projection capabilities in the West Pacific. Multi-dimensional force buildup, especially precisely guided intermediate and cruise missiles could impose a formidable anti-access and area-denial barrier for the US naval and air power. As a response, the US Pentagon has come up with a new doctrinal breakthrough, namely, AirSea Battle concept. In an effort to offset the numerical and geographical advantage of the PLA, the US has set about integrating its air and sea power components. By generating maximum cross-domain synergy effect, the Pentagon believes that China`s ambition to challenge the freedom of navigation would be discouraged. America`s regional allies as Japan and Korea will benefit from the US resolve to remain committed to the regional balance of power. Regional economies heavily dependent on trade and cross-national investment will continue to prosper as freedom of navigation in the sea and the air is guaranteed. Yet, the dissenters of the new doctrinal concept present various negative evaluation of America`s response to missile capabilities buildup of the PLA. The expensive program is seen to only end up accelerating the dangerous arms race in the West Pacific. Against this backdrop, careful cost-effect analyses as to AirSea battle concept are required to respond to the changing strategic environment surrounding the Korean peninsula, This study has investigated and introduced the assessments underway in Japan, and attempted to present an answer whether and how much the AirSea battle concept can contribute to the security of the ROK in the years to come.

REVIEW : Intestinal Stricture in Crohn`s Disease

( Chen Wang Chang ),( Jau Min Wong ),( Chien Chih Tung ),( I Lun Shih ),( Horng Yuan Wang ),( Shu Chen Wei ) 대한장연구학회 2015 Intestinal Research Vol.13 No.1

Crohn`s disease (CD) is a disease with chronic inflammation of unknown etiology involving any part of the gastrointestinal tract. The incidence and prevalence of CD are increasing recently in Asia. Half of the CD patients will have intestinal complica-tions, such as strictures or fistulas, within 20 years after diagnosis. Twenty-five percentage of CD patients have had at least one small bowel stricture and 10% have had at least one colonic stricture and lead to significant complications. Most of these pa-tients will require at least one surgery during their lifetime. Early diagnosis and evaluation with adequate managements for the patients can prevent disability and mortality of these patient. Here, we reviewed the current incidence of CD with stricture, the etiology of stricture, and how to diagnose and manage the stricture. (Intest Res 2015;13:19-26)

Bivariate GARCH-Jump Model

Chuang-Chang Chang,Hsiao-Wei Ho,Tzu-Hsiang Liao,Yaw-Huei Wang 한국재무학회 2011 한국재무학회 학술대회 Vol.2011 No.09

In this paper we consider the pricing of quanto derivatives with the bivariate GARCH-Jump model, in which jumps take place in the price kernel, and consequently in foreign asset returns and in exchange rates. In the empirical investigation, we use Dow Jones, NASDAQ and NIKKEI 225 indexes, exchange rates and corresponding index warrants data to examine the effects of jump on derivative pricing. The empirical results suggest that the unrestricted bivariate NGARCH-Jump model outperforms the other four models considered in this study. The evidence also reveals that the average pricing error is the smallest for the unrestricted bivariate NGARCH-Jump model. Hence, the nonlinear asymmetric model with jumps captures the dynamics of index return and exchange rate well.