http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Shin, D.Y.,Sung Kang, H.,Kim, G.Y.,Kim, W.J.,Yoo, Y.H.,Choi, Y.H. Masson Pub. USA, Inc 2013 Biomedicine & pharmacotherapy Vol.67 No.4
Decitabine (5-aza-2'-deoxycytidine), an inhibitor of DNA methyltransferases, has a wide range of anti-metabolic and anti-cancer activities. Decitabine also induces cell cycle arrest at G2/M phase and apoptosis in human cancer cells. However, the cellular and molecular mechanisms of this cell cycle arrest are poorly understood. In the present study, we investigated the roles of the tumor suppressor p53 and the cyclin-dependent kinase (Cdk) inhibitor p21 following decitabine-induced G2/M arrest in human cancer cells. DNA flow cytometric analyses indicated that decitabine induced a G2/M arrest in AGS gastric and A549 lung carcinoma cell lines, which have wild type p53. Western blot analyses using whole cell lysates from AGS cells demonstrated that decitabine treatment did not change the steady-state level of Cdks and Cdk inhibitor p27, but it partially inhibited expression of cyclin A, cyclin B1, and Cdc25C proteins. However, similar results were found using the A549 cell line, where decitabine induced a dramatic up-regulation of both p53 and p21 expression, and the increased levels of p21 were associated with increased binding of p21 with Cdks, cyclin A, and cyclin B1. Knockdown of p53 by small interfering RNA (siRNA) markedly abolished p53 induction by decitabine in AGS cells, yet p53 siRNA had no attenuating effect on p21 induction. In addition, depletion of p21 expression with siRNA, but not p53, significantly attenuated decitabine-induced G2/M arrest. We also observed that decitabine strongly induced G2/M arrest associated with p21 induction in both p53 allele-null (-/-) HCT116 and wild type p53 (+/+) HCT116 cell lines. Therefore, our data indicated that p21 plays a crucial role in decitabine-induced G2/M arrest and operates in a p53-independent manner.
Identification of Novel SNPs in Bovine Insulin-like Growth Factor Binding Protein-3 (IGFBP3) Gene
Kim, J.Y.,Yoon, D.H.,Park, B.L.,Kim, L.H.,Na, K.J.,Choi, J.G.,Cho, C.Y.,Lee, H.K.,Chung, E.R.,Sang, B.C.,Cheong, I.J.,Oh, S.J.,Shin, Hyoung Doo Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.1
The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene.
2종의 엽록소 측정기를 이용한 포장내 벼의 엽록소 함량 분포 측정
성제훈,서상룡,박우풍,정인규,김상철,이충근 한국농업기계학회 2003 바이오시스템공학 Vol.28 No.3
This study was investigated the possibility of measuring chlorophyll in paddy crops using two kinds of chlorophyll meters(SPAD-502, Field Scout). The results of correlation analysis showed the 0.7758(r^2) relationship between the chlorophyll contents of paddy leaves measured by SPAD and spectro-photometer. It indicates that chlorophyll content in paddy crops could be measured using the SPAD. Considering the data of the SPAD and Field Scout, there was significant difference between their chlorophyll contents measured by the SPAD and Field Scout. Likewise, such results were consistent with the corrected data using light intensity. According to the results, it can be concluded that it is difficult to describe the rice chlorophyll measured by the Field Scout more accurately than the SPAD when using one standard spectrum and another spectrum for reflectance measuring. The chlorophyll variance measured at a research institute and a farmer's field revealed that the output of SPAD was more reliable than that of the Field Scout.
홀로그램 회절격자의 이동에 의한 포토폴리며의 위상 변위측정
성기영,경천수,김휘성,이진국,곽종훈,최옥식 嶺南大學校 基礎科學硏究所 1998 基礎科學硏究 Vol.18 No.-
Holographic recording and erasure kinetics in a home-made photopolymer film are investigated as a function of monomer concentration and spatial frequency. We also measured phase shifts of index grating and absorption grating by moving holographic photopolymer gratings along a direction of grating wave vector.
성백섭,박창언,김일수,차용훈,이진구 한국공작기계학회 2002 한국공작기계학회 춘계학술대회논문집 Vol.2002 No.-
This paper begin a new approach in the water-gate controller using radio communication. The dissertation is on the controllable device development of water-gate in the remote distance from water-gate trough the transceiver by radio communication. The proposed water-gate control device is simple in structure, an suitable for implementation of water-gate control in the remote distance. The remote controller water-gate device inspected the up and down motion of water-gate through the LCD displayer, so that it was very safety driving about the surroundings information, over loading and position of water-gate, and so on.