Statistical Optimization of Medium Components for Milk-Clotting Enzyme Production by Bacillus amyloliquefaciens D4 Using Wheat Bran-an Agro-Industry Waste

( Wei Bing Zhang ),( Xiao Ling He ),( Hong Na Liu ),( Hui Yuan Guo ),( Fa Zheng Ren ),( Wei Dong Gao ),( Peng Cheng Wen ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8

In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett?Burman experimental design. Subsequently, an optimal medium was obtained using the Box?Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4?7H2O, 0.1 g/l of MgSO4?7H2O, 0.1 g/l of MnSO4?2H2O, 0.1 g/l of ZnSO4?7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett?Burman design and Box?Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.

A large-area free-standing graphene oxide multilayer membrane with high stability for nanofiltration applications

Chen, Long,Li, Yanhui,Chen, Lina,Li, Na,Dong, Chenglong,Chen, Qiong,Liu, Beibei,Ai, Qing,Si, Pengchao,Feng, Jinkui,Zhang, Lin,Suhr, Jonghwan,Lou, Jun,Ci, Lijie Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.345 No.-

<P><B>Abstract</B></P> <P>A flexible and free-standing graphene oxide and nylon 6 (GO@nylon 6) multilayer nanofiltration membrane was prepared by a layer-by-layer assembly process. The combination of electrospinning and electrospraying technique was employed, which can facilely prepare large-area membrane with size of 20 × 30 cm. The mechanical stability of multilayer membrane has enhanced significantly due to the tightly locked structure achieved by nylon 6 nanofibers network. The novel GO@nylon 6–13 multilayer nanofiltration membrane demonstrated a pure water flux up to 11.15 L m<SUP>−2</SUP> h<SUP>−1</SUP> bar<SUP>−1</SUP>, while keeping high organic dye rejection rate (>95% for methylene blue, and >99% for methyl orange). The rejections rate of the Na<SUB>2</SUB>SO<SUB>4</SUB>, NaCl, CuSO<SUB>4</SUB>, and Pb(NO<SUB>3</SUB>)<SUB>2</SUB> were 56.5%, 27.6%, 36.7%, and 18.9%, respectively. Furthermore, GO@nylon 6–13 multilayer nanofiltration membrane also demonstrated a high flux of some common organic solvents (8.4, 5.3, and 0.8 L m<SUP>−2</SUP> h<SUP>−1</SUP> bar<SUP>−1</SUP> for methanol, ethanol, and NMP, respectively), showing excellent chemical stability for separation process in those solvents.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Large-area GO@nylon 6 multilayer nanofiltration membrane was prepared. </LI> <LI> The multilayer structure enhances the mechanical stability. </LI> <LI> The multilayer membrane demonstrates a high water flux. </LI> <LI> The multilayer membrane shows high rejection rate for organic dyes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Photograph and cross-section SEM image of GO@nylon 6 multilayer nanofiltration membrane, the inset shows the water contact angle.</P> <P>[DISPLAY OMISSION]</P>

A Multi-center Survey of HPV Knowledge and Attitudes Toward HPV Vaccination among Women, Government Officials, and Medical Personnel in China

Zhao, Fang-Hui,Tiggelaar, Sarah M.,Hu, Shang-Ying,Zhao, Na,Hong, Ying,Niyazi, Mayinuer,Gao, Xiao-Hong,Ju, Li-Rong,Zhang, Li-Qin,Feng, Xiang-Xian,Duan, Xian-Zhi,Song, Xiu-Ling,Wang, Jing,Yang, Yun,Li, Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Objectives: To assess knowledge of HPV and attitudes towards HPV vaccination among the general female population, government officials, and healthcare providers in China to assist the development of an effective national HPV vaccination program. Methods: A cross-sectional epidemiologic survey was conducted across 21 urban and rural sites in China using a short questionnaire. 763 government officials, 760 healthcare providers, and 11,681 women aged 15-59 years were included in the final analysis. Data were analyzed using standard descriptive statistics and logistic regression. Results: Knowledge of HPV among the general female population was low; only 24% had heard of HPV. Less than 20% of healthcare providers recognized sexually na$\ddot{i}$ve women as the most appropriate population for HPV vaccination. There was high acceptance of the HPV vaccine for all categories of respondents. Only 6% of women were willing to pay more than US $300 for the vaccine. Conclusions: Aggressive education is necessary to increase knowledge of HPV and its vaccine. Further proof of vaccine safety and efficacy and government subsidies combined with increased awareness could facilitate development and implementation of HPV vaccination in China.

Susceptibility Loci Associations with Prostate Cancer Risk in Northern Chinese Men

Wang, Na-Na,Xu, Yong,Yang, Kuo,Wei, Dong,Zhang, Yao-Guang,Liu, Ming,Shi, Xiao-Hong,Liang, Si-Ying,Sun, Liang,Zhu, Xiao-Quan,Yang, Yi-Ge,Tang, Lei,Zhao, Cheng-Xiao,Wang, Xin,Chen, Xin,Hui, Juan,Zhang, Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Background: KLK3 gene products, like human prostate-specific antigen (PSA), are important biomarkers in the clinical diagnosis of prostate cancer (PCa). G protein-coupled receptor RFX6, C2orf43 and FOXP4 signaling plays important roles in the development of PCa. However, associations of these genes with PCa in northern Chinese men remain to be detailed. This study aimed to investigate their impact on occurrence and level of malignancy. Methods: All subjects were from Beijing and Tianjin, including 266 cases with prostate cancer and 288 normal individuals as controls. We evaluated associations between clinical covariates (age at diagnosis, prostate specific antigen, Gleason score, tumor stage and aggressive) and 6 candidate PCa risk loci, genotyped by PCR- high resolution melting curve and sequencing methods. Results: Case-control analysis of allelic frequency of PCa associated with PCa showed that one of the 6 candidate risk loci, rs339331 in the RFX6 gene, was associated with reduced risk of prostate cancer (odds ratio (OR) = 0.73, 95% confidence interval (CI) =0.57-0.94, P = 0.013) in northern Chinese men. In addition, subjects with CX (CC+TC) genotypes had a decreased risk for prostrate cancer compared to those carrying the TT homozygote (OR =0.64, 95% CI = 0.45- 0.90, P = 0.008). The TT genotype of 13q22 (rs9600079, T) was associated with tumor stage (P=0.044, OR=2.34, 95% CI=0.94-5.87). Other SNPs were not significantly associated with clinical covariates in prostate cancer (P > 0.05). Conclusions. rs339331 in the RFX6 gene may be associated with prostate cancer as a susceptibility locus in northern Chinese men.

Transcriptome analysis of the pheromone glands in Noorda blitealis reveals a novel AOX group of the superfamily Pyraloidea

Zhang Zu-Bing,Yin Ning-Na,Long Ji-Ming,Zhang Yong-Ke,Liu Nai-Yong,Zhu Jiaying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Noorda blitealis (Lepidoptera: Pyraloidea: Crambidae) is a major defoliating pest of Moringa trees. Focusing on its mating and reproduction, here we sequenced and analyzed the transcriptome of its pheromone glands (PGs) with a combination of Illumina sequencing, bioinformatics and phylogenetics approaches, coupled with a genomebased analysis. Transcriptome sequencing led to the yields of approximately 162 million clean reads, which were assembled into 60,578 unigenes and 121,692 transcripts, respectively. From the transcriptome, totally 117 genes encoding eight pheromone biosynthesis enzymes and one pheromone degradation enzyme were identified, 90 of which had complete open reading frames. A comparative analysis between PGs and bodies (removing PGs) revealed a large number of differentially expressed genes, including 79 pheromone biosynthesis and degradation related genes. Of the identified genes, NbliDES12 belonging to the △11 desaturase group was likely to a strong candidate for the desaturation of sex pheromones in N. blitealis, as implied by phylogenetic analyses and expression profiles. Finally and most notably, through genome and transcriptome analyses we discovered, for the first time, a novel aldehyde oxidase 6 (AOX6) group of the superfamily Pyraloidea that have been slightly expanded by gene duplications. Moreover, each orthologous AOX group shared highly conserved gene structure. Together, this current study has characterized the genes associated with sex pheromone biosynthesis and degradation from the PG transcriptome of N. blitealis, and more importantly, identifies a novel AOX group of the Pyraloidea.

기숙사 가구의 인간 중심적 리디자인 연구 - H 대학을 중심으로 -

( Zhang Na ),( Ko Jeong Hoon ) 한국상품문화디자인학회(구 한국패키지디자인학회) 2020 상품문화디자인학연구 Vol.61 No.-

대학생들은 편안한 집에서 여러 사람이 생활하는 기숙사로 이사를 하며, 개인의 생활 방식, 행동 습관 및 그들의 심리는 환경의 변화에 영향을 받을 것이다. 또한 기숙사 가구 사용은 학생 숙박 경험에 직접적인 영향을 미칠 것이다. 이에 본 연구는 하이난의 H 대학의 기숙사 가구를 대학생의 욕구를 충족시키기 위해, 숙소의 필수 요소와 라이프 스타일 및 행동 상태를 인간 중심의 관점에서 조사 분석하였다. 이에 이론적 연구 및 설계 실습을 통해 이 현재 가구의 유형을 정리하였다. 기숙사 가구의 현재 상태, 기능 요구 사항 및 결함에 따라 H 대학의 기숙사 가구 디자인 요소, 디자인 원칙 및 디자인 계획을 제안하였다. 본 연구를 통해 기숙사 가구는 그 구조와 기숙사를 이용하는 학생 및 게스트의 라이프 스타일과 행동 유형에 적지 않은 영향을 주었으며, 조사 및 분석을 중심으로 한 가구의 디자인이 필수적으로 나타났다. 또한 제안한 가구 디자인을 통한 결과물이 사용자의 니즈에 충분히 충족하는 결과가 도출되었다. College students move from comfortable homes to dormitories with multiple people. Individuals' lifestyle, behavioral habits, and their mentality will be affected by changes in the environment. Also the use of dormitory furniture will directly affect the student accommodation experience. This study takes the dormitory furniture of H University in Hainan, according to the accommodation requirements, lifestyles and behavioral status of dormitories of college students, from human centered perspective to explore and analyze the dormitory furniture that meets the needs of college students. Through theoretical research and design practice, the current types of furniture is summarized. According to the current status, functional requirements, and deficiencies of dormitory furniture, the design elements, design principles, and design schemes for the dormitory furniture of H universities are proposed. Through this study, dormitory furniture had a significant influence on the structure and lifestyle and behavioral patterns of students and guests using the dormitory, and design of furniture based on research and analysis was essential. Also, the result through the proposed furniture design satisfies the user's needs.

An Exploration of Interior Design of Rural Tourism of Folk Dwellings in Hainan Province of China

ZHANG NA 인문사회과학예술융합학회 2018 인문사회과학예술융합학회지 Vol.2 No.2

The paper studies the interior design of rural tourism of folk dwellings in Hainan Province of China, to Serving the construction of "Beautiful Village, Windy Town" in .Hainan International Tourism Island. It mainly discusses the current situation and existing problems of interior design of rural residential houses in Hainan, exploring the principles and methods of interior design of rural tourism houses in Hainan,and issues such as the expression of regional culture Key words: Hainan; Rural tourism; Residence; Interior Design

海南乡村旅游民居室内设计探索

( Zhang Na ) 인문사회과학예술융합학회 2018 인문사회과학예술융합학회지 Vol.2 No.2

The paper studies the interior design of rural tourism of folk dwellings in Hainan Province of China, to Serving the construction of "Beautiful Village, Windy Town" in .Hainan International Tourism Island. It mainly discusses the current situation and existing problems of interior design of rural residential houses in Hainan, exploring the principles and methods of interior design of rural tourism houses in Hainan,and issues such as the expression of regional culture.

Evaluation of storage period of fresh ginseng for quality improvement of dried and red processed varieties

Na Zhang,Xin Huang,Yun-Long Guo,Hao Yue,Chang-Bao Chen,Shu-Ying Liu 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.2

Background: Dried and red ginseng are well-known types of processed ginseng and are widely used as healthy food. The dried and red ginseng quality may vary with the storage period of raw ginseng. Therefore, herein, the effect of the storage period of fresh ginseng on processed ginseng quality was evaluated through multicomponent quantification with statistical analysis. Methods: A method based on ultrahigh performance liquid chromatography coupled to triple quadrupole mass spectrometry in multiple-reaction monitoring mode (UPLC-MRM-MS) was developed for quantitation of ginsenosides and oligosaccharides in dried and red ginseng. Principal component analysis and partial least squares discriminant analysis were conducted to evaluate the dynamic distributions of ginsenosides and oligosaccharides after different storage periods. Results: Eighteen PPD, PPT and OLE ginsenosides and nine reducing and nonreducing oligosaccharides were identified and quantified. With storage period extension, the ginsenoside content in the processed ginseng increased slightly in the first 2 weeks and decreased gradually in the following 9 weeks. The content of reducing oligosaccharides decreased continuously as storage time extending, while that of the nonreducing oligosaccharides increased. Chemical conversions occurred during storage, based on which potential chemical markers for the storage period evaluation of fresh ginseng were screened. Conclusion: According to ginsenoside and oligosaccharide distributions, it was found that the optimal storage period was 2 weeks and that the storage period of fresh ginseng should not exceed 4 weeks at 0 °C. This study provides deep insights into the quality control of processed ginseng and comprehensive factors for storage of raw ginseng.

Linear Optimal Estimation for Discrete-time and Continuous-time Systems with Multiple Measurement Delays

Na-Na Jin,Shuai Liu,Huan-Shui Zhang 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.3

In this paper, we investigate the linear optimal estimation problems of discrete-time and continuous-time systems with multiple state delays in measurements. For discrete-time systems, we obtain the linear optimal estimation of state by direct calculation of optimal gain in terms of the solution to a retarded Riccati-like difference equation instead of a group of Riccati difference equations. For continuous-time systems, we also obtain the analytical expression of linear optimal estimation without resorting to Riccati partial differential equations. All the Riccati equations are of the same dimension as the system to be estimated and the computational cost is much saved. Infinite horizon case is also studied by stability analysis. Kalman filter can be recovered from our result when delays disappear. A numerical example is provided to demonstrate the results.