<i>Haloactinopolyspora alkaliphila</i> sp. nov., and emended description of the genus <i>Haloactinopolyspora</i>

Zhang, Yong-Guang,Liu, Qing,Wang, Hong-Fei,Zhang, Dao-Feng,Zhang, Yuan-Ming,Park, Dong-Jin,Kim, Chang-Jin,Li, Wen-Jun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.6

<P>A facultatively alkaliphilic actinomycete strain, designated EGI 80088<SUP>T</SUP>, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088<SUP>T</SUP> formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained <SMALL>ll</SMALL>-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, summed feature 4 (iso-C<SUB>17 : 1</SUB>I/anteiso-C<SUB>17 : 1</SUB>B), iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The predominant menaquinone was MK-9(H<SUB>4</SUB>). The G+C content of the genomic DNA of strain EGI 80088<SUP>T</SUP> was 70.6 mol%. EGI 80088<SUP>T</SUP> showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour <I>Haloactinopolyspora alba</I> YIM 93246<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088<SUP>T</SUP> and <I>H. alba</I> YIM 93246<SUP>T</SUP> was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088<SUP>T</SUP> represents a novel species of the genus <I>Haloactinopolyspora</I>, for which the name <I>Haloactinopolyspora alkaliphila</I> sp. nov. (type strain EGI 80088<SUP>T</SUP> = BCRC 16946<SUP>T</SUP> = JCM 19128<SUP>T</SUP>) is proposed. The description of the genus <I>Haloactinopolyspora</I> has also been emended.</P>

Melatonin Attenuates Noise Stress-induced Gastrointestinal Motility Disorder and Gastric Stress Ulcer: Role of Gastrointestinal Hormones and Oxidative Stress in Rats

( Lei Zhang ),( Ji T Gong ),( Hu Q Zhang ),( Quan H Song ),( Guang H Xu ),( Lei Cai ),( Xiao D Tang ),( Hai F Zhang ),( Fang E Liu ),( Zhan S Jia ),( Hong W Zhang ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims There are increasing evidences for gastrointestinal motility disorder (GIMD) and gastric stress ulcer induced by noise stress. The present study was to investigate the reversed effect of melatonin on GIMD and gastric stress ulcer induced by noise stress and potential mechanism. Methods Noise stress was induced on rats, and melatonin (15 mg/kg) was administered to rats by intraperitoneal injection. Differences were assessed in gastric residual rate (GRR), small intestine propulsion rate (SPR), Guth injury score, cortisol, gastrointestinal hormones (calcitonin-gene-related peptide and motilin) and oxidative stress markers (superoxide dismutase and malondialde hyde) in blood plasma as well as gastric mucosa homogenate with or without melatonin. The pathological examination of gastric mucosa was also performed. Results The GRR and SPR were improved by noise stress compared with control (P < 0.05). The pathological examination and Guth injury score revealed gastric stress ulcer. Moreover, the levels of cortisol, motilin and malondialdehyde in blood plasma and malondialdehyde in gastric mucosa homogenate were increased by noise stress (P < 0.05). CGRP and superoxide dismutase activity in both of blood plasma and gastric mucosa homogenate were significantly decreased (P< 0.05). Furthermore, melatonin reversed changes in GRR, SPR, pathological examination, Guth injury score, cortisol, motilin, CGRP, superoxide dismutase activity and malondialdehyde (P < 0.05). Conclusions Melatonin is effective in reversing the GIMD and gastric stress ulcer induced by noise stress. The underlying mechanism may be involved in oxidative stress and gastrointestinal hormones. (J Neurogastroenterol Motil 2015;21:189-199)

Two New Species of the Genus Allozelotes Yin & Peng, 1998 (Araneae, Gnaphosidae) from China

Yang Zi-Zhong, Zhang Zi-Sheng, Zhang Yao-Guang, Joo Pil Kim(김주필) 한국거미연구소 2009 한국거미 Vol.25 No.1-2

중국 Yunnan 지역에서 채집한 수리거미과의 Allozelotes 속의 2 신종을 기재하여 보고하는 바이다. 즉 Allozelotes songi sp. nov.와 A. microsaccatus sp. nov. 이다. 표본은 SWUC와 CDLC에 보관한다. 측정치는 millimeters이다. Two new species of the genus Allozelotes collected from Yunnan, China, are described and illustrated: Allozelotes songi sp. nov. and A. microsaccatus sp. nov. Type specimens are respectively deposited in School of Life Science, Southwest University(SWUC) and College of Life Science and Chemistry, Dali University(CDLC). All measurements given are in millimeters.

Effect of the opening of a butterfly valve on the dynamic evolution of cavitation

Guang Zhang,Ze Yong Wu,Ke Xin Wu,Yu Qiong Ou,Heuy Dong Kim,Zhe Lin 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

As the key control equipment for the transmission of fluid medium, processing valves are widely used in the transmission systems of fluid medium in energy, chemical industry, metallurgy and other fields, which play important roles in the stability and reliability of the system operation. When the flow cross-section is operated in a sudden change, the pressure decreases rapidly at the downstream, which leads to the cavitation in the processing valves. Cavitation makes serious erosion and damage on the valve core and pipeline surface, which makes the leakage and noise problems in processing valves. This seriously affects the regulation performance and lifetime of processing valves. In this article, numerical simulations were carried out to investigate the transient cavitation in a model butterfly valve. By considering effects of local pressure on formation of cavitation, a modified model for calculating the diameter of cavitation bubbles was derived. Effects of valve opening degree were investigated on the dynamic evolution of cavitation by analyzing formation, development and collapse of cavitation. The generation, development and collapse of single cavitation bubble was obtained and discussed in details to state the interaction between vortices and cavitation. Attached and quasiperiodic cavitation were observed and analyzed at different valve opening degrees in detail as well.

Numerical analysis of impulse waves discharged at the exit of a model tunnel

Guang Zhang,김동현,김희동 대한기계학회 2018 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.32 No.5

Impulse waves are micro-pressure waves, which always occur at the tunnel exit when a high-speed train is moving inside a train tunnel. The air around the train nose is compressed and compression waves are induced. The impulse wave is discharged at the exit of a train tunnel when a compression wave propagates outside of the tunnel exit. Impulse waves are weak-strength pressure waves, which lead to noise and other environmental problems. In order to efficiently control the impulse wave at the exit of a train tunnel, numerical studies on investigating the generation and propagation of the impulse wave were carried out. A 2-D axisymmetric model tunnel was simulated at different operating conditions. Different Mach numbers of compression waves were varied to induce different magnitudes of impulse waves at the tunnel exit. In addition, compression waves with different pressure gradients were assumed at the tunnel entry to check their effects on the generation of impulse waves. In order to observe impulse waves at far field, five monitor points were installed behind the tunnel exit to record pressure histories as impulse waves moved through these locations. The detailed magnitudes and characteristics of impulse waves were obtained in the present studies.

Oxidized Acetate Starch as a New Surface Sizing Agent

Zhang Guang-hua,Lai Zhi-chao,Li Hui,Wang Zhou-ni 한국펄프·종이공학회 2009 펄프.종이기술 Vol.41 No.5

SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin–Rho GTPase–Hippo Pathways

Zhang, Yan-Li,Li, Qing,Yang, Xiao-Mei,Fang, Fang,Li, Jun,Wang, Ya-Hui,Yang, Qin,Zhu, Lei,Nie, Hui-Zhen,Zhang, Xue-Li,Feng, Ming-Xuan,Jiang, Shu-Heng,Tian, Guang-Ang,Hu, Li-Peng,Lee, Ho-Young,Lee, Su-J American Association for Cancer Research 2018 Cancer research Vol.78 No.9

<P>Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P>Tumor-associated macrophages (TAM) represent key regulators of the complex interplay between cancer and the immune microenvironment. Matricellular protein SPON2 is essential for recruiting lymphocytes and initiating immune responses. Recent studies have shown that SPON2 has complicated roles in cell migration and tumor progression. Here we report that, in the tumor microenvironment of hepatocellular carcinoma (HCC), SPON2 not only promotes infiltration of M1-like macrophages but also inhibits tumor metastasis. SPON2-α4β1 integrin signaling activated RhoA and Rac1, increased F-actin reorganization, and promoted M1-like macrophage recruitment. F-Actin accumulation also activated the Hippo pathway by suppressing LATS1 phosphorylation, promoting YAP nuclear translocation, and initiating downstream gene expression. However, SPON2-α5β1 integrin signaling inactivated RhoA and prevented F-actin assembly, thereby inhibiting HCC cell migration; the Hippo pathway was not noticeably involved in SPON2-mediated HCC cell migration. In HCC patients, SPON2 levels correlated positively with prognosis. Overall, our findings provide evidence that SPON2 is a critical factor in mediating the immune response against tumor cell growth and migration in HCC.</P><P><B>Significance:</B> Matricellular protein SPON2 acts as an HCC suppressor and utilizes distinct signaling events to perform dual functions in HCC microenvironment.</P><P><B>Graphical Abstract:</B> http://cancerres.aacrjournals.org/content/canres/78/9/2305/F1.large.jpg. <I>Cancer Res; 78(9); 2305–17. ©2018 AACR</I>.</P><P><B>Graphical Abstract</B></P><P> [Figure]</P>

Tax is Involved in Up-regulation of HMGB1 Expression Levels by Interaction with C/EBP

Zhang, Chen-Guang,Wang, Hui,Niu, Zhi-Guo,Zhang, Jing-Jing,Yin, Ming-Mei,Gao, Zhi-Tao,Hu, Li-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

The high mobility group box 1 (HMGB1) protein is a multifunctional cytokine-like molecule that plays an important role in the pathogenesis of tumors. In this study, real-time polymerase chain reactions and Western blot assays indicated that HMGB1 transcriptional activity and protein level are increased in $Tax^+$-T cells (TaxP). To clarify the mechanisms, a series of HMGB1 deletion reporter plasmids (pHLuc1 to pHLuc6) were transfected into $Tax^-$-T cells (TaxN, Jurkat) and $Tax^+$-T cells (TaxP). We found that promoter activity in $Tax^+$-T cells to be higher than that in $Tax^-$-T cells, indicating a significant increase in pHLuc6. Bay11-7082 (NF-${\kappa}B$ inhibitor) treatment did not block the enhancing effect. Chromatin immunoprecipitation assays revealed that Tax was retained on a HMGB1 promoter fragment encompassing -1163 to -975. Bioinformatics analysis showed six characteristic cis-elements for CdxA, AP-1, AML-1a, USF, v-Myb, and C/EBP in the fragment in question. Mutation of cis-elements for C/EBP reduced significant HMGB1 promoter activity induced by Tax. These findings indicate that Tax enhances the expression of HMGB1 gene at the transcriptional level, possibly by interacting with C/EBP.

Lack of Association of Common Polymorphisms in MUC1 Gene with H. pylori Infection and Non-cardia Gastric Cancer Risk in a Chinese Population

Zhang, Bin,Hao, Guang-Yu,Gao, Fang,Zhang, Jian-Zu,Zhou, Cheng-Jiang,Zhou, Li-She,Wang, Ying,Jia, Yan-Bin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Several lines of evidence support the notion that MUC1 is often aberrantly expressed in gastric cancer, and it is a ligand for Helicobacter pylori. Genetic variation in MUC1 gene may confer susceptibility to H. pylori infection and gastric cancer. We assessed the association of common polymorphisms in MUC1 gene with H. pylori infection and non-cardia gastric cancer using an LD-based tag SNP approach in north-western Chinese Han population. A total of four SNPs were successfully genotyped among 288 patients with non-cardia gastric cancer and 281 age- and sex-matched controls. None of the tested SNPs was associated with H. pylori infection. SNP rs9426886 was associated with a decreased risk of non-cardia gastric cancer, but lost significance after adjustment for multiple testing. Overall, our data indicated that common genetic variations in MUC1 gene might not make a major contribution to the risk of H. pylori infection and non-cardia gastric cancer in our studied population.

Experimental Study on the Shock Wave Propagation in a Drug Delivery Device

Guang Zhang,Ik In Lee,Heuy Dong Kim 한국가시화정보학회 2015 한국가시화정보학회 학술발표대회 논문집 Vol.2015 No.12

Recently, the needle-free delivery system has been widely used in medical fields due to its convenience in delivering drug particles into human body without any external needles. In order to penetrate through the outer layer of the skin, drug particles need to obtain enough momentum, which is achieved by accelerating drug particles in a Contoured Shock Tube (CST). The main component of the CST is a micro shock tube with two diaphragms. In the present study, experimental studies were carried out by pressure measurement in a micro shock tube. Five high sensitive pressure transducers were used for recording pressure changes as the shock wave moves through different locations along tube walls in the test section. From which, data on shock wave propagation can be obtained. Different diaphragm pressure ratios were conducted to demonstrate effects of initial diaphragm pressure ratios on shock wave propagation. Shilieren visualization was also performed to observe shock wave propagation and shock wave structure in the present experimental shock tube model. The characteristic of the internal flow and shock wave system have been studied and analyzed in details in the present shock tube model.