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Axisymmetric deformation in transversely isotropic thermoelastic medium using new modified couple stress theory
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TEM studies of precipitates in creep deformed 9-10%Cr steels
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Lu Yunjun,Li Shu,Shen Xiaodong,Zhao Yan,Zhou Dongming,Hu Dan,Cai Xushen,Lu Lixia,Xiong Xiaohui,Li Ming,Cao Min 한국미생물학회 2021 The journal of microbiology Vol.59 No.10
Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that presents a significant threat both to pigs and to workers in the pork industry. The initial steps of S. suis 2 pathogenesis are unclear. In this study, we found that the type II histidine triad protein HtpsC from the highly virulent Chinese isolate 05ZYH33 is structurally similar to internalin A (InlA) from Listeria monocytogenes, which plays an important role in mediating listerial invasion of epithelial cells. To determine if HtpsC and InlA function similarly, an isogenic htpsC mutant (ΔhtpsC) was generated in S. suis by homologous recombination. The htpsC deletion strain exhibited a diminished ability to adhere to and invade epithelial cells from different sources. Double immunofluorescence microscopy also revealed reduced survival of the ΔhtpsC mutant after cocultivation with epithelium. Adhesion to epithelium and invasion by the wild type strain was inhibited by a monoclonal antibody against E-cadherin. In contrast, the htpsC-deficient mutant was unaffected by the same treatment, suggesting that E-cadherin is the host-cell receptor that interacts with HtpsC and facilitates bacterial internalization. Based on these results, we propose that HtpsC is involved in the process by which S. suis 2 penetrates host epithelial cells, and that this protein is an important virulence factor associated with cell adhesion and invasion.
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Shuang Qiu,Yunjun Yan 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
We describe a new Tinodes, Tinodes stamen sp. nov., collected in the Dabie Mountains, East-central China andreport four new records, T. ventralis Li & Morse, 1997; T. cryptophallicata Li & Morse, 1997; T. harael Malicky,2017; and T. sartael Malicky, 2017, from Dabie Mountains. Moreover, we illustrate infraspecific variability in T. ventralis, and the recently described T. harael, and T. sartael from the Dabie Mountains region.
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( Wenjuan Yang ),( Li Xu ),( Houjin Zhang ),( Yunjun Yan ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.11
In this study, Pseudomonas R0-14, which was isolated from Arctic soil samples, showed a clear halo when grown on M9 medium agarose plates containing olive oil-rhodamine B as substrate, suggesting that it expressed putative lipase(s). A putative lipase gene, lipR, was cloned from R0-14 by genome walking and Touchdown PCR. lipR encodes a 562-amino-acid polypeptide showing a typical α/β hydrolase structure with a catalytic triad consisting of Ser153-Asp202-His260 and one α-helical lid (residues 103-113). A phylogenetic analysis revealed that LipR belongs to the lipase subfamily I.3. LipR was successfully expressed in Escherichia coli, purified, and biochemically characterized. Recombinant LipR exhibited its maximum activity towards p-nitrophenyl butyrate at pH 8.5 and 60oC with a Km of 0.37 mM and a kcat of 6.42 s-1. It retained over 90% of its original activity after incubation at 50oC for 12 h. In addition, LipR was activated by Ca2+, Mg2+, Ba2+, and Sr2+, while strongly inhibited by Cu2+, Zn2+, Mn2+, and ethylenediaminetetraacetic acid. Moreover, it showed a certain tolerance to organic solvents, including acetonitrile, isopropanol, acetone, methanol, and tert-butanol. When algal oil was hydrolyzed by LipR for 24 h, there was an enrichment of n-3 long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (1.22%, 1.65-fold), docosapentaenoic acid (21.24%, 2.04-fold), and docosahexaenoic acid (36.98%, 1.33-fold), and even a certain amount of diacylglycerols was also produced. As a result, LipR has great prospect in industrial applications, especially in food and/or cosmetics applications.
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Resolution of Racemic Ketoprofen in Organic Solvents by Lipase from Burkholderia cepacia G63
Xiang-lin Zhang,Tao Liu,Li Xu,Xiaohua Gui,Feng Su,Yunjun Yan 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.6
A lipase from the Burkholderia cepacia strain G63 immobilized on resin was used for the resolution of ketoprofen. To study its catalytic properties in enantioselective esterication, different alcohols and solvents were tested to select the most suitable acyl acceptor and reaction medium. Compared with the low activity of the free lipase, the enzyme activity and E value of the immobilized lipase were significantly enhanced. The enantioselectivity of the immobilized lipase could also be markedly improved by adding a small amount of 18-crown-6. RSM was employed to optimize the reaction parameters. The optimal reaction conditions were: reaction time 22.50 h, additives dosage 0.4322 g (0.33 mmol/mL), and substrate molar ratio 54.11:1. Under optimal conditions, the maximal E value was up to 10.01, which exhibited a better enantioselectivity than some commercial lipases, such as Novozym 435,Lipozyme RM IM and LipozymeTL IM.
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