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- 저자 : Yu Honglian (검색결과 2 건)
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Wang Xinzhe,Li Siqi,Yu Honglian,Lv Jianzhi,Fan Minglun,Wang Ximing,Wang Xin,Liang Yanting,Mao Lingna,Zhao Zhankui 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.3
BACKGROUND: Nowadays, biological tissue engineering is a growing field of research. Biocompatibility is a key indicator for measuring tissue engineering biomaterials, which is of great significance for the replacement and repair of damaged tissues. METHODS: In this study, using gelatin, carboxymethyl chitosan, and sodium alginate, a tissue engineering material scaffold that can carry cells was successfully prepared. The material was characterized by Fourier transforms infrared spectroscopy. In addition, the prepared scaffolds have physicochemical properties, such as swelling ratio, biodegradability. we observed the biocompatibility of the hydrogel to different adult stem cells (BMSCs and ADSCs) in vivo and in vitro. Adult stem cells were planted on gelatin-carboxymethyl chitosan-sodium alginate (Gel/SA/CMCS) hydrogels for 7 days in vitro, and the survival of stem cells in vitro was observed by live/died staining. Gel/SA/CMCS hydrogels loaded with stem cells were subcutaneously transplanted into nude mice for 14 days of in vivo culture observation. The survival of adult stem cells was observed by staining for stem cell surface markers (CD29, CD90) and Ki67. RESULTS: The scaffolds had a microporous structure with an appropriate pore size (about 80 lm). Live/died staining showed that adult stem cells could stably survive in Gel/SA/CMCS hydrogels for at least 7 days. After 14 days of culture in nude mice, Ki67 staining showed that the stem cells supported by Gel/SA/CMCS hydrogel still had high proliferation activity. CONCLUSION: Gel/SA/CMCSs hydrogel has a stable interpenetrating porous structure, suitable swelling performance and degradation rate, can promote and support the survival of adult stem cells in vivo and in vitro, and has good biocompatibility. Therefore, Gel/SA/CMCS hydrogel is a strong candidate for biological tissue engineering materials.
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Xiaoying Li,Shuang Yu,Pibo Ma,Gaoming Jiang,Honglian Cong 한국섬유공학회 2019 Fibers and polymers Vol.20 No.9
In order to study the compression property of curved three-dimensional(3D) flat-knitted spacer fabric compositesand to provide experimental data for the study of subsequent low speed impact and residual strength, 3D flat-knitted spacerfabrics were knitted into flat and curvilinear shapes, then 3D flat-knitted spacer fabric composites curved in different angleswere developed. Compression tests on various curvilinear composites were carried out using the universal material testingmachine after which stress-strain curves are compared. The results revealed that when other experimental conditions are thesame, the coursewise compression peak was significantly higher than waleswise, and compression peak also graduallyincreased with the increase of curvature, no matter the surface of 3D spacer fabric reinforced composites is flat or curvilinear. This research will lay the foundation for the further mechanical property study of curved 3D spacer fabric composites.
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