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      • KCI등재

        Flavonoid Production Is Effectively Regulated by RNAi Interference of Two Flavone Synthase Genes from Glycine max

        Yi Na Jiang,Biao Wang,Hui Li,Lu Ming Yao,Tian Long Wu 한국식물학회 2010 Journal of Plant Biology Vol.53 No.6

        Flavonoids are a group of secondary metabolites found in many higher plants. The multiple roles of their flavone subclass include protection against UV damage,regulation of auxin transport, and modulation of flower color. In soybean (Glycine max), flavone synthase II (FNS II) is the key enzyme responsible for flavone biosynthesis. Two FNS II genes from soybean cultivar Hefeng 47 were cloned according to basic local alignment search tool (BLAST) contexts using flavone synthase sequences reported in other species. These were named GmFNSII-1and GmFNSII-2. Sequence alignments showed that the cDNA of GmFNSII-1 was identical to that of CYP93B16,whereas GmFNSII-2 was clearly distinct. Functional assays in yeast (Schizosaccharomyces pombe) suggested that these two enzymes could convert (2S)-naringenin into apigenin. The two GmFNSII genes had similar tissue-specific expression patterns, but GmFNSII-2 was significantly expressed in the roots after treatment with 0.4 M glucose. This demonstrates that the gene plays an important role in the response to defense signals in soybean. RNA interference-mediated suppression of those GmFNSII genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes (ATCC15834). Our study also highlights some of the challenges associated with metabolic engineering of plant natural products.

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        Fabricating Fe₃O₄ and Fe₃O₄&Fe Flower-like microspheres for Electromagnetic Wave Absorbing in C and X Bands

        Na Chen,Dan Li,Xin‑Yi Wang,Zhen‑Jie Guan,Jian‑Tang Jiang,Kang‑Jun Wang 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.4

        Magnetic composites have received increasing attention for electromagnetic wave absorption (EMA) applications. However,the practical EMA performance of the materials is severely hampered by mismatching impedance characteristics and finiteelectromagnetic attenuation capacity. Controlling the components and building the architecture fabrication is necessary tosolve these issues. Herein, a series of Fe3O4,Fe3O4&Fe and Fe microspheres with flower-like hierarchical structures wereconstructed through a solvothermal method followed by an annealed process. This hierarchical structure and the synergyeffect of dielectric dissipation and magnetic loss capacity offer Fe3O4a perfect impedance matching, providing an excellentEMA performance of an effective absorption bandwidth (EAB) of 4.0 GHz and a reflection loss (RL) of 67.9 dB. Meanwhile,the coordination of the hierarchical structures and the multiple components endow Fe3O4&Fe composites with an EAB aswide as 5.7 GHz (9.0–14.7 GHz) and a RL as strong as 78.7 dB at 1.88 mm, which covers 75% X and 45% Ku bands. Sucha remarkable lightweight and broad properties is due to the decent X band impedance matching and appropriate attenuationcapacity. Therefore, this work highlights the significant of regulating the hierarchical structure and components to enhancethe EMA performances.

      • KCI등재

        Triterpenoid saponins from Clinopodium chinense (Benth.) O. Kuntze and their biological activity

        Yin-Di Zhu,Jing-Yi Hong,Feng-Da Bao,Na Xing,Ling-Tian Wang,Zhong-Hao Sun,Yun Luo,Hai Jiang,Xudong Xu,Nai-Liang Zhu,Hai-Feng Wu,Gui-Bo Sun,Jun-Shan Yang 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.12

        Four new ursane-type triterpenoid saponins, clinopoursaponins A–D (1–4), six new oleanane-type triterpenoid saponins, clinopodiside VII–XII (5–10), as well as eight known triterpene analogues (11–18), were isolated from the aerial parts of Clinopodium chinense (Benth.) O. Kuntze. The structures of the new compounds were determined based on extensive spectral analyses, including 1D (1H and 13C) and 2D NMR experiments (COSY, NOESY, HSQC, 2D TOCSY, HSQC-TOCSY and HMBC), HR-ESI-MS and chemical methods. Compounds 1–18 were evaluated for their protective effects against anoxia/reoxygenation-induced apoptosis in H9c2 cells and cytotoxicities against murine mammary carcinoma cell line 4T1. Compounds 8, 9 and 18 exhibited significant protective effects, while compound 1 exhibited cytotoxic activity with IC50 value of 7.4 μm compared to 7.6 μm for the positive control 10-hydroxycamptothecin.

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