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      • KCI등재

        Research on air diversion channel of air-cooled gasoline engine cylinder head and simulation of fluid- solid coupling heat transfer

        Du B. C.,Tang G. Z.,Zhang L.,Zhong L.,Deng T.,Yi Y. L. 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.11

        In view of the problem that local temperature of the original cylinder head is on the high side, an innovative cooling air diversion channel is proposed. The cooling air diversion channel introduces cool air into the cylinder head to forcedly cool the cylinder head. Furthermore, the multiple guide plates are set to reasonably distribute cold air in the air diversion channel. In addition, appropriately increase the length of the fins to cooling the whole cylinder head. The fluid-solid coupling three-dimensional simulation technology is adopted to analyze the impact of the new air diversion channel on the heat dissipation of cylinder head. Finally, the optimal scheme is defined to fabricate the prototype. Simulations results show that the above improvements make the temperature in the high temperature region effectively decrease, and the cooling performance of whole cylinder head is enhanced. In the optimal improvement scheme, the highest temperature of cylinder head decrease by 23 K. Furthermore, the prototype was fabricated according to the optimal scheme. The experimental results show that the temperature of the improved cylinder head decreased effectively, and the highest temperature decreased by 20.4 K. The simulation results are in agreement with the experimental results, and the maximum error is less than 5 percent.

      • SCIESCOPUSKCI등재

        Effect of Dietary Energy Level on Nutrient Utilization, Insulin-like Growth Factor-I and Insulin-like Growth Factor Binding Protein-3 in Plasma, Liver and Longissimus dorsi Muscle in Growing-finishing Pigs Using Soybean Oil as an Energy Source

        Du, W.,Li, Y.J.,Zhao, G.Y.,Yin, Y.L.,Kong, X.F. Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.8

        Two experiments were carried out to study the effects of dietary energy level on nutrient digestion, nitrogen (N) utilization, growth performance, insulin-like growth factor-I (IGF-I), and insulin-like growth factor binding protein-3 (IGFBP-3) in plasma, liver and longissimus dorsi muscle in growing-finishing pigs. In experiment 1 (Exp 1), 15 castrated male pigs (Duroc${\times}$Landrace${\times}$Large White) (Body weight, BW, 55.6${\pm}$1.8 kg) were divided into three groups and fed rations containing 13.33, 14.87 and 17.35 MJ digestible energy (DE)/kg as treatments I, II and III, respectively, using soybean oil as an energy source. The experiment lasted 8 days and faecal and urinary samples were collected during the last 3 days. The results showed that the digestibility of dry matter (DM), energy and N was increased from treatments I to III (p<0.01). N-retention and N-retention rate were not influenced by dietary DE level (p>0.05). In experiment 2 (Exp 2), 36 female pigs (Duroc${\times}$Landrace${\times}$Large White) (BW 41.5${\pm}$3.8 kg) were divided into three groups. The pigs were fed with the same three rations used in Exp 1 for 60 days. At the end of Exp 2, eight pigs were selected from each group for blood sampling and 4 pigs for slaughter trial. The results indicated that average daily feed intake (ADFI) and N-intake were significantly decreased (p<0.01), and DE intake (p<0.01) and average daily gain (ADG) (p<0.05) were increased. IGF-I and IGFBP-3 in plasma were increased (p<0.05). No significant differences in IGF-I and IGFBP-3 in liver and longissimus dorsi muscle were found between different treatments. It was concluded that higher dietary DE level improved nutrient digestibility, ADG and feed/gain ratio when soybean oil was used as an energy source in the ration of growing-finishing pigs. No significant differences were found in Nretention and IGF-I and IGFBP-3 in liver and longissimus dorsi muscle between different treatments.

      • SCIESCOPUSKCI등재

        Associations of T→A Mutation in the Promoter Region of Myostatin Gene with Birth Weight in Yorkshire Pigs

        Jiang, Y-L,Li, N,Fan, X-Z,Xiao, L-R,Xiang, R-L,Hu, X-X,Du, L-X,Wu, C-X Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.11

        A T$\longrightarrow$A mutation in the promoter region of porcine myostatin (MSTN) gene has been identified in our previous work. This study analyzed the associations of the myostatin genotypes (TT, TA) caused by this mutation with birth weigh in Yorkshire pigs. Data from 211 unrelated individuals were collected three times from one breeding farm. Detections of the mutation were carried out by PCR-RFLPs approach. The effects of MSTN genotypes (TT and TA) on birth weight were compared by least square means. The results showed that for birth weight of Yorkshire pigs, individuals with TA genotype were significantly higher than those with TT genotype (p<0.05), and the birth weight for pigs with TA genotype were 1.37 kg in average but only 1.25 kg for pigs with TT genotype, indicating a positive effect of birth weight for A allele.

      • SCIESCOPUSKCI등재

        Detection of 881<sup>A</sup>→881<sup>G</sup> Mutation in Tyrosinase Gene and Associations with the Black Ear Coat Color in Rabbits

        Jiang, Y.L.,Fan, X.Z.,Lu, Z.X.,Tang, H.,Xu, J.-Q.,Du, L.-X. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.10

        The tyrosinase gene was selected as a candidate for uncovering genetic mechanism causing 'black ear' coat color in rabbits. A PCR-SSCP detection method was established for the $881^A{\rightarrow}881^G$ mutation located in the central region of the tyrosinase gene between the CuA and CuB binding region signatures, and this was confirmed by sequencing and alignment. Fully consistent associations between the SNP and 'black ear' coat color were observed by analysis in a "black ear" pedigree and on 61 unrelated individuals. This SNP can serve as a molecular marker for use in "back ear" wool rabbit breeding.

      • SCISCIESCOPUS

        In vitro reconstitution of the cyclosporine specific P450 hydroxylases using heterologous redox partner proteins

        Sun, Y.,Ma, L.,Han, D.,Du, L.,Qi, F.,Zhang, W.,Sun, J.,Huang, S.,Kim, E. S.,Li, S. SPRINGER 2017 Journal of industrial microbiology & biotechnology Vol.44 No.2

        <P>The cytochrome P450 enzymes (CYPs) CYP-sb21 from Sebekia benihana and CYP-pa1 from Pseudonocardia autotrophica are able to hydroxylate the immunosuppressant cyclosporin A (CsA) in a regioselective manner, giving rise to the production of two hair-stimulating agents (with dramatically attenuated immunosuppressant activity), gamma-hydroxy-N-methyl-l-Leu4-CsA (CsA-4-OH) and gamma-hydroxy-N-methyl-l-Leu9-CsA (CsA-9-OH). Recently, the in vitro activity of CYP-sb21 was identified using several surrogate redox partner proteins. Herein, we reconstituted the in vitro activity of CYP-pa1 for the first time via a similar strategy. Moreover, the supporting activities of a set of ferredoxin (Fdx)/ferredoxin reductase (FdR) pairs from the cyanobacterium Synechococcus elongatus PCC 7942 were comparatively analyzed to identify the optimal redox systems for these two CsA hydroxylases. The results suggest the great value of cyanobacterial redox partner proteins for both academic research and industrial application of P450 biocatalysts.</P>

      • SCIESCOPUSKCI등재

        Optimal Design for Marker-assisted Gene Pyramiding in Cross Population

        Xu, L.Y.,Zhao, F.P.,Sheng, X.H.,Ren, H.X.,Zhang, L.,Wei, C.H.,Du, L.X. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.6

        Marker-assisted gene pyramiding aims to produce individuals with superior economic traits according to the optimal breeding scheme which involves selecting a series of favorite target alleles after cross of base populations and pyramiding them into a single genotype. Inspired by the science of evolutionary computation, we used the metaphor of hill-climbing to model the dynamic behavior of gene pyramiding. In consideration of the traditional cross program of animals along with the features of animal segregating populations, four types of cross programs and two types of selection strategies for gene pyramiding are performed from a practical perspective. Two population cross for pyramiding two genes (denoted II), three population cascading cross for pyramiding three genes(denoted III), four population symmetry (denoted IIII-S) and cascading cross for pyramiding four genes (denoted IIII-C), and various schemes (denoted cross program-A-E) are designed for each cross program given different levels of initial favorite allele frequencies, base population sizes and trait heritabilities. The process of gene pyramiding breeding for various schemes are simulated and compared based on the population hamming distance, average superior genotype frequencies and average phenotypic values. By simulation, the results show that the larger base population size and the higher the initial favorite allele frequency the higher the efficiency of gene pyramiding. Parents cross order is shown to be the most important factor in a cascading cross, but has no significant influence on the symmetric cross. The results also show that genotypic selection strategy is superior to phenotypic selection in accelerating gene pyramiding. Moreover, the method and corresponding software was used to compare different cross schemes and selection strategies.

      • SCIESCOPUS

        Multi-dimensional wind vibration coefficients under suction for ultra-large cooling towers considering ventilation rates of louvers

        Ke, S.T.,Du, L.Y.,Ge, Y.J.,Tamura, Y. Techno-Press 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.66 No.2

        Currently, the dynamic amplification effect of suction is described using the wind vibration coefficient (WVC) of external loads. In other words, it is proposed that the fluctuating characteristics of suction are equivalent to external loads. This is, however, not generally valid. Meanwhile, the effects of the ventilation rate of louver on suction and its WV are considered. To systematically analyze the effects of the ventilation rate of louver on the multi-dimensional WVC of ultra-large cooling towers under suctions, the 210 m ultra-large cooling tower under construction was studied. First, simultaneous rigid pressure measurement wind tunnel tests were executed to obtain the time history of fluctuating wind loads on the external surface and the internal surface of the cooling tower at different ventilation rates (0%, 15%, 30%, and 100%). Based on that, the average values and distributions of fluctuating wind pressures on external and internal surfaces were obtained and compared with each other; a tower/pillar/circular foundation integrated simulation model was developed using the finite element method and complete transient time domain dynamics of external loads and four different suctions of this cooling tower were calculated. Moreover, 1D, 2D, and 3D distributions of WVCs under external loads and suctions at different ventilation rates were obtained and compared with each other. The WVCs of the cooling tower corresponding to four typical response targets (i.e., radial displacement, meridional force, Von Mises stress, and circumferential bending moment) were discussed. Value determination and 2D evaluation of the WVCs of external loads and suctions of this large cooling tower at different ventilation rates were proposed. This study provides references to precise prediction and value determination of WVC of ultra-large cooling towers.

      • SCIESCOPUS

        A study on the average wind load characteristics and wind-induced responses of a super-large straight-cone steel cooling tower

        Ke, S.T.,Du, L.Y.,Ge, Y.J.,Zhao, L.,Tamura, Y. Techno-Press 2017 Wind and Structures, An International Journal (WAS Vol.25 No.5

        As a novel typical wind-sensitive structure, the wind load and wind-induced structural behaviors of super-large straight-cone cooling towers are in an urgent need to be addressed and studied. A super large straight-cone steel cooling tower (189 m high, the highest in Asia) that is under construction in Shanxi Power Plant in China was taken as an example, for which four finite element models corresponding to four structural types: the main drum; main drum + stiffening rings; main drum + stiffening rings + auxiliary rings (auxiliary rings are hinged with the main drum and the ground respectively); and main drum + stiffening rings + auxiliary rings (auxiliary rings are fixed onto the main drum and the ground respectively), were established to compare and analyze the dynamic properties and force transferring paths of different models. After that, CFD method was used to conduct numerical simulation of flow field and mean wind load around the cooling tower. Through field measurements and wind tunnel tests at home and abroad, the reliability of using CFD method for numerical simulation was confirmed. On the basis of this, the surface flow and trail characteristics of the tower at different heights were derived and the wind pressure distribution curves for the internal and external surfaces at different heights of the tower were studied. Finally, based on the calculation results of wind-induced responses of the four models, the effects of stiffening rings, auxiliary rings, and different connecting modes on the dynamic properties and wind-induced responses of the tower structure were derived and analyzed; meanwhile, the effect mechanism of internal suction on such kind of cooling tower was discussed. The study results could provide references to the structure selection and wind resistance design of such type of steel cooling towers.

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