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Plasma treatment of multi-walled carbon nanotubes for lipase immobilization
Xun Cao,Rui Zhang,Wei-min Tan,Ce Wei,Jing Wang,Ze-meng Liu,Ke-quan Chen,Ping-Kai Ouyang 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.5
Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTslipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters.
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Development of Integrated Electro-Hydraulic Braking System and Its ABS Application
Zhi-Hui Tan,Zhen-Fu Chen,Xiao-Fei Pei,Xue-Xun Guo,Shuang-Hong Pei 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.3
This paper presented a new type of integrated EHB system. The EHB consisted of a compact three-chamber structure of master cylinder and mode switching valves. This allowed for the easy implementation of three different modes: normal mode, failsafe mode and ABS mode. In normal mode, a PWM control method was proposed for pressure regulation that took into account overshoot and hysteresis. To provide a favorable pedal feeling, a pedal stroke simulator was designed and integrated with the master cylinder. The failsafe and ABS modes were verified and the performance of the EHB in the three modes was evaluated using co-simulation and a bench test.
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High-Strength Model Material Production for Structural Plane Replica and Its Shear Testing
Feng Ji,Changjiang Liu,Yu Zhang,Luobing Zheng,Kai Pan,Xun Tan 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.1
Shear strength parameters (c, φ) of a structural plane are the key factors for the stability assessment of rock masses. The shear strength parameters are obtained by a structure plane shear test carried out in laboratory. However, it is difficult to obtain test samples with the same surface morphology and to remove the effect of surface irregularity from test results. Based on the similarity principle and orthogonal test, this paper presents a new model preparation method for structural planes using a high-strength mold mixture material. Firstly, the original morphology of the structural plane is recorded by in situ measurement using a laser device and then the mold of the structural plane is reproduced using a 3D printer. Subsequently, a mix proportion test of the high-strength mold material is conducted using the orthogonal test, and the model of the structural plane is prepared by pouring this high-strength mixture material into the 3D mold. Ultimately, the shear strength parameters of this high-strength structural plane replica are obtained using a shear box test in the laboratory. The proposed method has particular advantages such as the preparation of multiple replicas for structure planes and the ability to obtain repeatable results.
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