Genome-wide identification, characterisation and expression profile analysis of DEAD-box family genes in sweet potato wild ancestor Ipomoea trifida under abiotic stresses

Rong Wan,Jingran Liu,Zhengmei Yang,Panpan Zhu,Qinghe Cao,Tao Xu 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background DEAD-box protein family is the largest subfamily of RNA helicases and plays an important role in RNA metabolism and plant growth, development, and stress responses. Although DEAD-box genes have been characterized in various major crop plants, their identification and characterization in Convolvulaceae is still in infancy. Sweet potato (Ipomoea batatas, in Convolvulaceae) is the seventh most important crop in the world. Ipomoea trifida is one of the ancestors of sweet potato and is an effective resource for sweet potato cross-breeding. Objective Identification and characterisation of DEAD-box transcription factors in sweet potato wild ancestor I. trifida genome. Method A systematic genome-wide analysis was conducted to identify the DEAD-box transcription factors in the I. trifida genome. Results We identified 17 ItfDEAD-box genes which distributed unevenly on the nine chromosomes of I. trifida and encoded 29 DEAD transcripts. The phylogenetic analysis classified the DEAD-box proteins into nine groups named I–IX. Homology model prediction of ItfDEAD-box proteins obtained 14 models which lay a preliminary foundation for the further functional exploration of the ItfDEAD-box proteins. The tissue-specific and abiotic stress-responsive expression profiles of ItfDEAD-box genes were analyzed in different tissues and under abiotic stress responses by RNA-seq data and confirmed by quantitative PCR analysis. Some genes were significantly up- or down-regulated by different abiotic stress, suggesting that ItfDEAD-box plays a crucial role in stress responses in I. trifida. Conclusion The identification and gene expression of the ItfDEAD-box gene family might shed light on the function exploration of DEAD-box gene in I. trifida and promote the molecular breeding of sweet potato.

온톨로지 기반 시맨틱 블로그 모델의 OWL 변환 및 관심 블로그 커뮤니티 추천 기법 (pp.385-389)

허영화(Rong-Hua Xu),양경아(Kyung-Ah Yang),양재동(Jae-Dong Yang),최완(Wan Choi) 한국정보과학회 2009 정보과학회 컴퓨팅의 실제 논문지 Vol.15 No.5

As a new community forming environment, the blog platform enables sharing of the resources in blogosphere through active information exchange. Many researches have been performed to recommend appropriate resources to users from vast amounts of blog resources. As one of the solutions OSEM defines theknowledgebase in the blogosphere with ontology for effectively modeling it. In this paper, we propose a technique of converting the knowledgebase into the OWL ontology for sharing it on the semantic web environment. An inference method is then applied to the OWL ontology for recommending interest blog communities. For this aim, a mapping method is offered and then SWRL inference and SPARQL query based on the ontology are employed to extract interest blog communities. 최근 블로그 사용이 급증하면서 방대한 양의 블로그 자원으로부터 적합한 자원을 추출하여 사용자에게 제공하기 위한 연구들이 시도되었다. 이들 연구 중 OSEM(Ontology-based Semantic Blog Model)은 블로그 공간(Blogosphere) 내 지식베이스를 효과적으로 모델링하기 위해 이를 온톨로지로 정의한 모델이다. OSEM을 시맨틱 웹환경에서 활용하기 위해 본 논문에서는 매핑 기법을 적용하여 OSEM의 지식베이스를 OWL로 변환하고, OWL로 변환된 온톨로지에 SWRL 추론과 SPARQL 질의를 적용하여 블로그 사용자에게 유용한 관심 커뮤니티를 추천하는 기법을 제안한다. 제안 기법은 OSEM 내 지식베이스를 OWL로 변환함으로써 시맨틱 웹 환경에서의 공유와 재사용을 가능하게 하고, 제안한 추론 기법을 적용하여 사용자 관점의 관심 블로그 커뮤니티 추천을 가능하도록 한다.

Colorectal Cancer Incidence and Mortality in China, 2010

Zheng, Zhao-Xu,Zheng, Rong-Shou,Zhang, Si-Wei,Chen, Wan-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: The National Central Cancer Registry of China (NCCR) affiliated to the Bureau of Disease Control, National Health and Family Planning Commission of China is responsible for cancer surveillance in the entire country. Cancer registration data from each local registry located in each province are collected by NCCR annually to be analyzed and published to provide useful information for policy makers and cancer researchers. Materials and Methods: Until 1st June, 2013, 219 population-based cancer registries submitted data of 2010 to the National Central Cancer Registry of China covering about 207,229,403 population, and 145 cancer registries were selected after quality evaluation for this study. Colorectal cancer cases were selected from the database according to ICD-10 coded as "C18-C20". We calculated the crude incidence and mortality rates by sex, age groups and location (urban/rural). The China population in 2000 and Segi's population were used as standardized populations for the calculation of age-standardized rates. The 6th National Population Census data of China was used to combined with the cancer registries' data to estimate the colorectal cancer burden in China in 2010. Results: Colorectal cancer was the sixth most common cancer in China. It was estimated that there were 274,841 new cases diagnosed in 2010 (157,355 in males and 117,486 in females), with the crude incidence rate of 20.1/100,000, highest in males in urban areas. Age-standardized rates by China standard population of 2000 (ASRcn) and World standard population (Segi's population, ASRwld) for incidence were 16.1/100,000 and 15.9/100,000 respectively. There were 132,110 cases estimated to have died from colorectal cancer in China in 2010 (76,646 men and 55,464 women) with the crude mortality rate of 10.1/100,000. The ASRcn and ASRwld for mortality were 7.55/100,000 and 7.44/100,000 respectively, higher in males and urban areas than in females and rural areas. The incidence and mortality rates increased with age, reaching peaksin the 80-84 year old, and oldest age groups, respectively. Conclusions: Colorectal cancer is one of the most common incident cancers and cause of cancer death in China. Primary and secondary prevention, with attention to a health lifestyle, physical activity and screening should be enhanced in the general population.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Fa-Wu Dong,Huai-Rong Luo,Qin-Li Wan,Feng-Qing Xu,Weiwei Fan,Kai-Jin Wang,Ning Li,Jiang-Miao Hu 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-β-D-glucopyranoside (1) and 3,4,4',5- tetramethoxybibenzyl-3'-O-β-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).

Modeling of ion diffusion coefficient in saturated concrete

Xiao-Bao Zuo,Wei Sun,Cheng Yu,Xu-Rong Wan 사단법인 한국계산역학회 2010 Computers and Concrete, An International Journal Vol.7 No.5

This paper utilizes the modified Davis model and the mode coupling theory, as parts of the electrolyte solution theory, to investigate the diffusivity of the ion in concrete. Firstly, a computational model of the ion diffusion coefficient, which is associated with ion species, pore solution concentration, concrete mix parameters including water-cement ratio and cement volume fraction, and microstructure parameters such as the porosity and tortuosity, is proposed in the saturated concrete. Secondly, the experiments, on which the chloride diffusion coefficient is measured by the rapid chloride penetration test,have been carried out to investigate the validity of the proposed model. The results indicate that the chloride diffusion coefficient obtained by the proposed model is in agreement with the experimental result. Finally, numerical simulation has been completed to investigate the effects of the porosity, tortuosity, water-cement ratio, cement volume fraction and ion concentration in the pore solution on the ion diffusion coefficients. The results show that the ion diffusion coefficient in concrete increases with the porosity, water-cement ratio and cement volume fraction, while we see a decrease with the increasing of tortuosity. Meanwhile, the ion concentration produces more obvious effects on the diffusivity itself, but has almost no effects on the other ions.

Two New Bibenzyl Glucosides from Dendrobium chrysotoxum

Dong, Fa-Wu,Luo, Huai-Rong,Wan, Qin-Li,Xu, Feng-Qing,Fan, Wei-Wei,Wang, Kai-Jin,Li, Ning,Hu, Jiang-Miao Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.7

Two new bibenzyl glucosides, 3,3',4',5-tetramethoxybibenzyl-4-O-${\beta}$-D-glucopyranoside (1) and 3,4,4',5-tetramethoxybibenzyl-3'-O-${\beta}$-D-glucopyranoside (2), together with five known ones, chrysotobibenzyl (3), erianin (4), chrysotoxine (5), gigantol (6) and tristin (7) were isolated from the stems of Dendrobium chrysotoxum. The structures of those compounds were elucidated by extensive spectroscopic analysis. Moreover, compounds 1-7 were assessed for inhibitory activity of two enzymes-AChE (acetylcholine esterase) and BChE (butyrylcholine esterase).