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        Luzhong mutton sheep: inbreeding and selection signatures

        Lin Tao,Xiaoyun He,Fengyan Wang,Yingjie Zhong,Linxiang Pan,Xiangyu Wang,Shangquan Gan,Ran Di,Ming-xing Chu 한국축산학회 2020 한국축산학회지 Vol.62 No.6

        Intense artificial selection has been imposed to Luzhong mutton sheep population in the past years. Improvements on growth and reproductive performance are two breeding goals in the present herd. Although some progresses were phenotypically observed possibly due to inbreeding induced by strong selection in terms of these traits, the genomic evaluation was poorly understood. Therefore, a high-density SNP array was used to characterize the pattern of runs of homozygosity (ROH), estimate inbreeding and inbreeding depressions on early growth performance and litter size based upon ROH, and scan positive selection signatures of recent population. Consequently, a low inbreeding level was observed which had negative effects on litter size, but not on early growth performance. And 160 genes were under selection, of which some were reported to be linked to several traits of sheep including body weight, litter size, carcass and meat quality, milk yield and composition, fiber quality and health, and the top genes were associated with growth (growth hormone [GH]- growth hormone receptor [GHR]- Insulin-like growth factor 1 [IGF1] axis) and litter size (bone morphogenic proteins [BMPs]-associated). The effectiveness of previous breeding measures was highlighted, but purging selection was proposed to alleviate the inbreeding depression on litter size, providing some genomic insights to breeding management of Luzhong mutton sheep.

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        A novel and effective approach to enhance the interfacial interactions of meta-aramid fibers

        Hui Zhang,Xiaoyun Du,Jiawei Liu,YunHong Bai,Jingyi Nie,Jiaojun Tan,Zhibin He,Meiyun Zhang,Jinbao Li,Yonghao Ni 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

        Meta-aramid paper exhibits some unique properties, including high temperature resistance, electricalinsulation. However, due to the inherent chemical inertness of aramid fibers, the interfacial bondingbetween fibers is weak, which negatively affect the properties of aramid paper. Herein, we report a simple,effective and scalable process for substantially improving the interfacial bonding between aramidfibers, thus, mechanical and insulating properties of the meta-aramid composite paper, and it wasachieved by surface coating of aramid paper with meta-aramid stock solution that contains N, N dimethylacetamide(DMAc)-CaCl2 and high molecular weight poly (m-phenylene isophthalamide) polymers. Results show that the tensile strength, modulus, internal bond strength and tearing index of the resultantall-PMIA paper increased by 83 %, 58 %, 173 % and 89 %, respectively, in comparison with those of the control. The breakdown strength of the improved aramid paper is 190 % higher than that of the control paper,attaining 26.46 KV/mm. This work provides a simple, economical, efficient, and scalable method toimprove the overall performance of meta-aramid paper, which has great potential to be implementedat the industrial scale.

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        Production and Optimization of a Kiwi Pectin Methylesterase Inhibitor in Pichia pastoris GS115

        Qian Liu,Wentao Xu,Shiwen Han,Dongyan Cao,Xiaoyun He,Kunlun Huang,Xiaohong Mei 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.6

        Gene sequence coding of a kiwi pectin methylesteraseinhibitor was optimized, synthesized, and expressedin Pichia pastoris GS115 based on P. pastoris preferredcodon usage. The expression level of the recombinantprotein (kwPMEI) increased by 89.74% after codonoptimization. Expression conditions of recombinant strainswere optimized. The highest production of kwPMEI wasachieved using 0.8% sorbitol (added every 24 h), 0.05%oleic acid (added at the beginning of induction), and 0.5%methanol (added every 12 h). kwPMEI was purified usingNi2+ chelating affinity chromatography and 17 mg of theprotein was harvested from 60 mL of a culture supernatant. Activity analysis showed that kwPMEI efficiently inhibitedthe activity of different plant PMEs. High expression levelsand purification of kwPMEI will promote applications infruit and vegetable juices.

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