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Congcong Guo,Xiaoyun Bai,Mamun Abdullah Al,Mohammad Nurul Azim Sikder,Henglong Xu 한국해양과학기술원 2019 Ocean science journal Vol.54 No.1
Biological trait analysis (BTA) of protozoan communities has been used a useful bioindicator of environmental quality status in marine ecosystems. To determine the optimal water depths for bioassessment using multiple functional traits of periphytic protozoa, community functioning was studied at different water depths in the Yellow Sea, northern China. The community-weighted means (CWM) of bacterivores with small sizes showed an increasing trend from surface layers to the deeper ones, while those of algivores with medium and large sizes revealed an opposite trend, i.e., a decreasing trend from surface layers to deeper ones. However, with those of predators were distributed at depths of 2 to 3.5 m. There was a significant difference in the functional patterns, except for those at depths of 2 to 3.5 m (P > 0.05). All diversity indices except FDiv generally peaked and leveled off at either 2 or 3.5 m although they decreased with depth. The results suggest that sampling at depths of 2 and 3.5 m may be an optimal strategy for assessing water quality using the functional pattern of protozoa in coastal ecosystems.
A novel and effective approach to enhance the interfacial interactions of meta-aramid fibers
Hui Zhang,Xiaoyun Du,Jiawei Liu,YunHong Bai,Jingyi Nie,Jiaojun Tan,Zhibin He,Meiyun Zhang,Jinbao Li,Yonghao Ni 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
Meta-aramid paper exhibits some unique properties, including high temperature resistance, electricalinsulation. However, due to the inherent chemical inertness of aramid fibers, the interfacial bondingbetween fibers is weak, which negatively affect the properties of aramid paper. Herein, we report a simple,effective and scalable process for substantially improving the interfacial bonding between aramidfibers, thus, mechanical and insulating properties of the meta-aramid composite paper, and it wasachieved by surface coating of aramid paper with meta-aramid stock solution that contains N, N dimethylacetamide(DMAc)-CaCl2 and high molecular weight poly (m-phenylene isophthalamide) polymers. Results show that the tensile strength, modulus, internal bond strength and tearing index of the resultantall-PMIA paper increased by 83 %, 58 %, 173 % and 89 %, respectively, in comparison with those of the control. The breakdown strength of the improved aramid paper is 190 % higher than that of the control paper,attaining 26.46 KV/mm. This work provides a simple, economical, efficient, and scalable method toimprove the overall performance of meta-aramid paper, which has great potential to be implementedat the industrial scale.
RNAi-Mediated Gene Silencing of Trcot1 Induces a Hyperbranching Phenotype in Trichoderma reesei
Fei Gao,Mengzhu Li,Weiquan Liu,Yingguo Bai,Tu Tao,Yuan Wang,Jie Zhang,Huiying Luo,Bin Yao,Huoqing Huang,Xiaoyun Su 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.2
Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of the Neurospora crassa COT1 kinase was discovered in T. reesei and designated TrCOT1, which is of 83.3% amino acid sequence identity. Functional disruption of Trcot1 in T. reesei by RNAi-mediated gene silencing resulted in retarded sporulation on potato dextrose agar and dwarfed colonies on minimal medium agar plates containing glucose, xylan, lactose, xylose, or glycerol as the sole carbon source. The representative mutant strain, SUS2/Trcot1i, also displayed reduced mycelia accumulation but hyperbranching in the MM glucose liquid medium, with hyphal growth unit length values decreased to 73.0 μm/tip compared to 239.8 μm/tip for the parent strain SUS2. The hyperbranching phenotype led to slightly but significantly increased cellulase secretion from 24 to 72 h in a batch culture. However, the cellulase production per unit of mycelial biomass was much more profoundly improved from 24 to 96 h.