Ginseng improves cognitive deficit via the RAGE/NF-kB pathway in advanced glycation end product-induced rats

Xiaobin Tan,Junfei Gu,Bingjie Zhao,Shuyuan Wang,Jiarui Yuan,Chunfei Wang,Juan Chen,Jiping Liu,Liang Feng,Xiaobin Jia 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.2

Background: Ginseng, the root of Panax ginseng (PG), is used widely as a herbal medicine to prevent andtreat various diseases. Panax ginseng has pharmacological effects on neurodegenerative diseases such asAlzheimer’s disease (AD). The present study evaluated the neuroprotective effects of PG and its possibleneuroprotective mechanisms in advanced glycation end product (AGE)-induced AD in a rat model. Methods: Advanced glycation end products were injected bilaterally into the CA3 region of the rats’brains. The Morris water maze test and step-down type passive avoidance test were performed toevaluate their memory and cognitive abilities. The oxidation indexes in the hippocampus were detected. Immunohistochemistry was conducted to visualize the receptors for advanced glycation end products(RAGEs) and nuclear factor-kappa-light-chain-enhancer of activated B cell (NF-kB). Results: Behavioral results showed that PG (1 g/kg, 0.5 g/kg, and 0.25 g/kg) significantly shortened theescape latency, remarkably increased the number of crossing times, significantly decreased the numberof errors, and prolonged the latency in rats with AGE-induced AD. Panax ginseng also significantlyreduced the malondialdehyde level, increased the glutathione content, and increased superoxide dismutaseactivity in the hippocampus. Panax ginseng significantly decreased the expression of RAGE andNF-kB. The blockade of anti-RAGE antibody could significantly reduce AGE-induced impairments andregulate these expressions. Conclusion: Our results demonstrated that PG significantly inhibits AGE-induced memory impairmentand attenuates Alzheimer-like pathophysiological changes. These neuroprotective effects of PG may beassociated with the RAGE/NF-kB pathway. Our results provided the experimental basis for applying PG inpreventing and treating AD.

Ginseng improves cognitive deficit via the RAGE/NF-κB pathway in advanced glycation end product-induced rats

Tan, Xiaobin,Gu, Junfei,Zhao, Bingjie,Wang, Shuyuan,Yuan, Jiarui,Wang, Chunfei,Chen, Juan,Liu, Jiping,Feng, Liang,Jia, Xiaobin The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.2

Background: Ginseng, the root of Panax ginseng (PG), is used widely as a herbal medicine to prevent and treat various diseases. Panax ginseng has pharmacological effects on neurodegenerative diseases such as Alzheimer's disease (AD). The present study evaluated the neuroprotective effects of PG and its possible neuroprotective mechanisms in advanced glycation end product (AGE)-induced AD in a rat model. Methods: Advanced glycation end products were injected bilaterally into the CA3 region of the rats' brains. The Morris water maze test and step-down type passive avoidance test were performed to evaluate their memory and cognitive abilities. The oxidation indexes in the hippocampus were detected. Immunohistochemistry was conducted to visualize the receptors for advanced glycation end products (RAGEs) and nuclear factor-kappa-light-chain-enhancer of activated B cell (NF-${\kappa}B$). Results: Behavioral results showed that PG (1 g/kg, 0.5 g/kg, and 0.25 g/kg) significantly shortened the escape latency, remarkably increased the number of crossing times, significantly decreased the number of errors, and prolonged the latency in rats with AGE-induced AD. Panax ginseng also significantly reduced the malondialdehyde level, increased the glutathione content, and increased superoxide dismutase activity in the hippocampus. Panax ginseng significantly decreased the expression of RAGE and NF-${\kappa}B$. The blockade of anti-RAGE antibody could significantly reduce AGE-induced impairments and regulate these expressions. Conclusion: Our results demonstrated that PG significantly inhibits AGE-induced memory impairment and attenuates Alzheimer-like pathophysiological changes. These neuroprotective effects of PG may be associated with the RAGE/NF-${\kappa}B$ pathway. Our results provided the experimental basis for applying PG in preventing and treating AD.

Perovskite-based mixed protonic–electronic conducting membranes for hydrogen separation: Recent status and advances

Huina Wang,Xiaobin Wang,Bo Meng,Xiaoyao Tan,Kee Shyuan Loh,Jaka Sunarso,Shaomin Liu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.60 No.-

Hydrogen share in the energy market has increased significantly in line with greater demand for zero emission fuel and the development of novel production routes via renewable resources. The application of mixed protonic–electronic conducting (MPEC) ceramic membrane within the hydrogen production process is an innovative route that enables high purity hydrogen production with low cost. This review provides readers a brief summary of the research efforts on MPEC ceramic membrane for hydrogen separation as well as the membrane reactor for hydrogen production and dehydrogenation or hydrogenation reactions. Most of the existing MPEC ceramic membranes come from either a single-phase or a dual-phase membrane. We discuss the working principles, the performances, the advantages and disadvantages, and the main issues of all these membranes. Major emphasis of the review is to cover the literature published in the last ten years since the earlier progress has been well documented by the previously existing reviews. We also put forward recommendations for future research direction in this topic.

A simple seed-embedded method to prepare ZIF-8 membranes supported on flexible PESf hollow fibers

Hangliao Zhang,Xiaobin Wang,Luyang Wei,Bo Meng,Xiaoyao Tan,Wanqin Jin,Shaomin Liu 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-

With intrinsic porous structure, unique chemical versatility and abundant application opportunities,MOF membranes have attracted substantial research enthusiasm. Most of these supported MOFmembranes are prepared on the rigid ceramic substrates. If the MOF membrane possessesflexibility, theapplication opportunities will be expanded. This paper presents the synthesis of ZIF-8 membranes on theflexible polyethersulfone (PESf) hollowfibers by using a facile seed-embedded method. The resultantmembranes were characterized by scanning electron microscopy, energy dispersive X-ray, X-raydiffraction and fourier transform infrared spectroscopy. The prepared membranes were in a continuousand compact structure and showed good molecular sieve performance of H2 over CO2, N2 and CH4 with H2permeance up to 1.110 5 mol m 2 s 1 Pa 1. Due to the preferential adsorption, ZIF-8/PESf compositemembrane exhibited high permeance of H2, N2, and CH4 compared to CO2, showing potential applicationsin efficient CO2 capture from industrial mixtures. The association between the gas permeation, theamount of embedded ZIF-8 crystals and the membrane thickness was also clarified. The reproducibilitywas significantly improved compared to the conventional secondary seeding method. Attributed to thestrong adhesion between the ZIF-8 and the PESf, the membranes displayed excellent stability andflexibility which could sustain bending up to 90 without breaking the ZIF-8 membrane. This seedembeddedmethod is not only simple, but also has good reproducibility and scalability.

Ginsenoside Rg3 nanoparticles with permeation enhancing based chitosan derivatives were encapsulated with doxorubicin by thermosensitive hydrogel and anti-cancer evaluation of peritumoral hydrogel injection combined with PD-L1 antibody

Wu Hao,Wei Guoli,Luo Lixia,Li Lingchang,Gao Yibo,Tan Xiaobin,Wang Sen,Chang Haoxiao,Liu Yuxi,Wei Yingjie,Song Jie,Zhang Zhenhai,Huo Jiege 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Combination of chemotherapy and immune checkpoint inhibitor therapy has greatly improved the anticancer effect on multiple malignancies. However, the efficiency on triple-negative breast cancer (TNBC) is limited, since most patients bear “cold” tumors with low tumor immunogenicity. Doxorubicin (DOX), one of the most effective chemotherapy agents, can induce immunogenic cell death (ICD) and thus initiating immune response.In this study, to maximize the ICD effect induced by DOX, chitosan and cell-penetrating peptide (R6F3)-modified nanoparticles (PNPs) loaded with ginsenoside Rg3 (Rg3) were fabricated using the self-assembly technique, followed by co-encapsulation with DOX based on thermo-sensitive hydrogel. Orthotopic tumor model and contralateral tumor model were established to observe the antitumor efficacy of the thermo-sensitive hydrogel combined with anti-PD-L1 immunotherapy, besides, the biocompatibility was also evaluated by histopathological.Rg3-PNPs strengthened the immunogenic cell death (ICD) effect induced by DOX. Moreover, the hydrogel co-loading Rg3-PNPs and DOX provoked stronger immune response in originally nonimmunogenic 4T1 tumors than DOX monotherapy. Following combination with PD-L1 blocking, substantial antitumor effect was achieved due to the recruitment of memory T cells and the decline of adaptive PD-L1 enrichment.The hydrogel encapsulating DOX and highly permeable Rg3-PNPs provided an efficient strategy for remodeling immunosuppressive tumor microenvironment and converting immune “cold” 4T1 into “hot” tumors.